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    • 35. 发明申请
    • PRODUCTION OF MULTIMERIC GLYCOPROTEINS THROUGH CHEMICAL COUPLING
    • 通过化学偶联生产多种糖蛋白
    • WO1992005188A1
    • 1992-04-02
    • PCT/US1991003080
    • 1991-05-03
    • BIOGEN, INC.PEPINSKY, R., BlakeROSA, Joseph, J.CHEN, Ling-Ling
    • BIOGEN, INC.
    • C07K03/06
    • C07K1/1077A61K38/00C07K1/10C07K14/705C07K14/70514
    • The present invention relates to processes for producing multimeric glycoproteins involving the steps of: 1) oxidizing a solution including glycosylated polypeptides to produce aldehyde-functional glycoproteins; 2) reacting the aldehyde-functional glycoproteins with an aminothiol compound to produce thiol-functional glycoproteins; and 3) chemically coupling two or more thiol-functional glycoproteins through a direct disulfide bond or through a homobifunctional cross-linking agent. This invention also relates to multimeric glycoproteins having the general formula: P1G1-(N1)-R1-S-X-S-R2-(N2)-G2P2, wherein: P1 and P2 represent the same or different polypeptides; G1 and G2 represent the same or different glycosyl moieties on the polypeptides P1 and P2, respectively; (N1) and (N2) represent, independently, a linkage between G1 and R1 or G2 and R2, respectively, through a secondary or tertiary amine nitrogen atom, i.e., (N1) and (N2) may either represent an imino (-NH-) or anaza (=N- or -N=) linkage between the glycosyl moieties (G1, G2) and the R1 and R2 moieties; R1 and R2 represent the same or different organic radicals of 1-16 carbon atoms or polypeptides of up to 20 amino acids; S is sulfur; and X is a direct bond or is a divalent organic radical of from 1-16 carbon atoms. This invention also relates to multimeric forms of soluble CD4, soluble LFA3 and soluble VCAM-1b proteins.
    • 本发明涉及产生多聚糖蛋白的方法,包括以下步骤:1)氧化包括糖基化多肽的溶液以产生醛官能糖蛋白; 2)使醛官能糖蛋白与氨基硫醇化合物反应以产生硫醇功能性糖蛋白; 和3)通过直接二硫键或通过同双功能交联剂化学偶联两种或多种巯基功能性糖蛋白。 本发明还涉及具有以下通式的多聚糖蛋白:P1G1-(N1)-R1-S-X-S-R2-(N2)-G2P2,其中P1和P2表示相同或不同的多肽; G1和G2分别代表多肽P1和P2上相同或不同的糖基部分; (N1)和(N2)分别独立地表示通过仲胺或叔胺氮原子分别在G1和R1或G2和R2之间的连接,即(N1)和(N2)可以表示亚氨基(-NH - )或糖基部分(G1,G2)和R1和R2部分之间的anaza(= N-或-N =)键; R1和R2表示1-16个碳原子的相同或不同的有机基团或多达20个氨基酸的多肽; S是硫; X是直接键或是1-16个碳原子的二价有机基团。 本发明还涉及可溶性CD4,可溶性LFA3和可溶性VCAM-1b蛋白的多聚体形式。
    • 39. 发明申请
    • METHOD AND DEVICE FOR REASSEMBLING A DATA FILE
    • 重新组织数据文件的方法和装置
    • WO2012026884A1
    • 2012-03-01
    • PCT/SG2011/000291
    • 2011-08-24
    • AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCHTHING, Ling Ling Vrizlynn
    • THING, Ling Ling Vrizlynn
    • G06F17/30
    • G06F17/30135G06F17/30117
    • Embodiments provide a method for reassembling a data file from a starting file fragment and a plurality of file fragments stored on a digital storage device. The method includes determining, from the plurality of file fragments, one or more matched file fragments which match the starting file fragment based on a first predetermined criterion; associating the one or more matched file fragments with the starting file fragment; and determining one or more candidate data files based on the one or more matched file fragments. The method further includes checking if more than one file fragments have been determined to match the starting file fragment based on the first predetermined criterion. If more than one matched file fragments have been determined to match the starting file fragment based on the first predetermined criterion, the method further includes selecting a candidate data file from the candidate data files determined for the matched file fragments as the reassembled data file based on a second predetermined criterion.
    • 实施例提供了一种从起始文件片段重新组合数据文件的方法和存储在数字存储设备上的多个文件片段。 该方法包括:从多个文件片段中,基于第一预定标准确定与起始文件片段匹配的一个或多个匹配文件片段; 将一个或多个匹配的文件片段与起始文件片段相关联; 以及基于所述一个或多个匹配的文件片段来确定一个或多个候选数据文件。 该方法还包括根据第一预定标准来检查是否确定了多于一个文件片段以匹配起始文件片段。 如果基于第一预定标准确定了多于一个匹配的文件片段以匹配起始文件片段,则该方法还包括从为匹配的文件片段确定的候选数据文件中选择候选数据文件作为重组的数据文件,基于 第二预定标准。