专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

21. 发明授权

US09139861B2 Culture substrate comprising cellulose gel, solid medium using same, and cellulase activity assay method using medium 有权
标题翻译：包含纤维素凝胶的培养基质，使用其的固体培养基和使用培养基的纤维素酶活性测定方法
公开(公告)号：US09139861B2
公开(公告)日：2015-09-22
申请号：US13881756
申请日：2011-10-24
申请人： Kazunori Nagaki , Yasunori Kurosawa , Takuo Shiraishi , Shigeru Deguchi , Mikiko Tsudome
发明人： Kazunori Nagaki , Yasunori Kurosawa , Takuo Shiraishi , Shigeru Deguchi , Mikiko Tsudome
IPC分类号： C12Q1/34 , C12N1/22 , C12Q1/04
CPC分类号： C12Q1/34 , C12N1/22 , C12N2533/78 , C12Q1/045 , G01N2333/942
摘要： A cellulose gel medium having good visibility of microbial colonies, a cellulose gel culture substrate for manufacturing the cellulose gel medium, a method for manufacturing the cellulose gel culture substrate, a method for screening cellulase-producing microorganisms or cellulase activity with greater efficiency and rapidity, and a culture substrate, which includes a cellulose gel containing cellulose and water as medium-solidifying components, the cellulose has the viscosity of 12 to 35 mPa·S as measured at 26° C. with a solution prepared by dissolving the cellulose at a concentration of 2.5 mg/mL in dimethylacetamide containing 8% (W/V) lithium chloride.
摘要翻译：具有微生物菌落的良好可见性的纤维素凝胶介质，用于制造纤维素凝胶介质的纤维素凝胶培养基质，纤维素凝胶培养基质的制造方法，以及更高的效率和快速地筛选纤维素酶生产微生物或纤维素酶活性的方法，和含有纤维素和水作为中固化成分的纤维素凝胶的培养基材，纤维素在26℃下测定的粘度为12〜35mPa·S，通过以浓度溶解纤维素制备的溶液在含有8％（W / V）氯化锂的二甲基乙酰胺中的浓度为2.5mg / mL。

22. 发明申请

US20150050680A1 Measuring Method of Enzyme Activity 审中-公开
标题翻译：酶活性测定方法
公开(公告)号：US20150050680A1
公开(公告)日：2015-02-19
申请号：US14380040
申请日：2013-02-20
申请人： Japan Agency for Marine-Earth Science and Technoloy , Kyokuto Pharmaceutical Industrial Co., Ltd.
发明人： Shigeru Deguchi , Mikiko Tsudome , Kazunori Nagaki , Nemuri Todaka , Yasunori Kurosawa
IPC分类号： C12Q1/40
CPC分类号： C12Q1/40 , C12Q1/00 , G01N2333/942
摘要： Providing is a new enzyme assay method for enzymes having a water-insoluble or substantially water-insoluble substrate. In the method for measuring enzymatic activity, a prescribed amount of an enzyme is disposed on a part of the surface of a gel comprising dispersoids, at least some of which are the substrate of the enzyme. Recesses formed in the surface of the gel by the action of the enzyme are measured, and the enzyme activity is calculated on the basis of the measurement results and the amount of the enzyme. The measurement of the recesses formed in the surface of the gel is performed using a method for measuring the shape and the volume of the recesses, a method for measuring changes in the optical transmittance of the gel due to the formation of the recesses, or a method for measuring changes in the optical reflectance of the gel surface due to the formation of the recesses.
摘要翻译：提供一种用于具有水不溶性或基本上不溶于水的底物的酶的新的酶测定方法。在测定酶活性的方法中，将规定量的酶设置在包含分散体的凝胶表面的一部分上，其中至少一些是酶的底物。测定通过酶的作用在凝胶表面形成的凹处，并根据测定结果和酶量计算酶活性。使用用于测量凹部的形状和体积的方法来测量形成在凝胶表面中的凹部，测量由于形成凹部而导致的凝胶的光透射率的变化的方法，或用于测量由于形成凹部而导致的凝胶表面的光学反射率变化的方法。

23. 发明申请

US20150017670A1 Methods For Determining The Degradation Of A Biomass Material 审中-公开
标题翻译：确定生物质材料降解的方法
公开(公告)号：US20150017670A1
公开(公告)日：2015-01-15
申请号：US14367194
申请日：2012-12-20
申请人： Novozymes, Inc.
发明人： Jason Quinlan , Brett McBrayer , Elena Vlasenko , David Osborn
IPC分类号： C12Q1/34
CPC分类号： C12Q1/34 , B01L3/5085 , B01L3/5635 , B01L2200/0642 , B01L2300/0829 , G01N33/0098 , G01N33/5097 , G01N2333/942
摘要： The present invention relates to methods for analyzing degradation of a biomass material at a high dry weight percentage.
摘要翻译：本发明涉及以高干重百分比分析生物质材料的降解的方法。

24. 发明申请

US20140302543A1 STRATEGY FOR THE ASSAY OF ENZYME AND ENZYME MIXTURES FOR THE HYDROLYSIS LIGNO-CELLULOSIC BIOMASS 审中-公开
标题翻译：用于水解微生物生物量的酶和酶混合物的测定策略
公开(公告)号：US20140302543A1
公开(公告)日：2014-10-09
申请号：US14359484
申请日：2012-11-22
申请人： C5-6 Italy S.R.L
发明人： Laura Volpati , Stefano Paravisi , Beatriz Rivas Torres
IPC分类号： C12Q1/34
CPC分类号： C12Q1/34 , G01N2333/924 , G01N2333/938 , G01N2333/942
摘要： The invention is to a novel method for assaying most or all of the activities of an enzyme or enzyme solution that could be used to convert ligno-cellulosic material into monomeric sugars. The method characterizes enzymes and enzyme mixtures for the hydrolysis of ligno-cellulosic biomass by measuring the protein concentration of an enzyme or enzyme mixture, adding the enzyme or enzyme mixture to a plurality of test vials creating a liquid fraction in the plurality of test vials wherein each test vial in the plurality of test vials contains a different substrate, incubating each test vial for a period of time sufficient to establish the conversion of the substrate in each test vial of the plurality of test vials at the same pH and temperature, while, if necessary, providing at least enough agitation to keep the substrate in suspension, quenching the reaction, and analyzing the optical absorbance of the liquid fraction of at least one test vial of the plurality of test vials.
摘要翻译：本发明是用于测定可用于将木质纤维素材料转化为单体糖的酶或酶溶液的大部分或全部活性的新方法。该方法表征酶和酶混合物，用于通过测量酶或酶混合物的蛋白质浓度来水解木质纤维素生物质，将酶或酶混合物加入到多个测试小瓶中，在多个测试小瓶中产生液体部分，其中多个测试小瓶中的每个测试小瓶含有不同的底物，将每个测试小瓶孵育足够的时间以在相同的pH和温度下建立多个测试小瓶的每个测试小瓶中的底物转化，如果需要，提供至少足够的搅拌以保持基质悬浮，淬灭反应，并分析多个测试小瓶的至少一个测试小瓶的液体部分的光吸收。

25. 发明申请

US20170335412A1 System and Methods For The Detection of Multiple Chemical Compounds 有权
公开(公告)号：US20170335412A1
公开(公告)日：2017-11-23
申请号：US15613692
申请日：2017-06-05
申请人： FredSense Technologies Corp.
发明人： Robert Matthew Mayall , Emily Candice Hicks , Margaret Mary-Flora Renaud-Young , David Christopher Lloyd , Lisa Kara Oberding , Iain Fraser Scotney George
IPC分类号： C12Q1/68 , C12Q1/34
CPC分类号： C12Q1/6897 , C12Q1/34 , G01N2333/938 , G01N2333/942 , G01N2458/30
摘要： Methods that may be used for the electrochemical detection of multiple parameters, including chemical compounds. Further provided are cells that may be used in the electrochemical detection of multiple parameters, including chemical compounds, as well as a kit for the electrochemical detection of multiple parameters, including chemical compounds.

26. 发明申请

US20160368894A1 SUBSTITUTED AMINO TRIAZOLES USEFUL AS ACIDIC MAMMALIAN CHITINASE INHIBITORS 审中-公开
标题翻译：取代的氨基三唑作为酸性乳清蛋白酶抑制剂有用
公开(公告)号：US20160368894A1
公开(公告)日：2016-12-22
申请号：US15254538
申请日：2016-09-01
申请人： OncoArendi Therapeutics Sp. z o.o.
发明人： Adam A. Golebiowski , Robert Koralewski , Wojciech J. Czestkowski , Krzysztof Matyszewski , Sylwia Olejniczak , Jacek P. Olczak , R. Paul Beckett
IPC分类号： C07D401/04 , G01N33/573
CPC分类号： C07D401/04 , C07D401/14 , G01N33/573 , G01N2333/942 , G01N2500/04 , G01N2800/122 , G01N2800/52
摘要： Disclosed are amino triazole compounds substituted by a carboxylate functional group or an bioisosteric polar functional group. Compounds having the carboxylate moiety or carboxylate bioisostere inhibit acidic mammalian chitinase. Also provided are methods of using the compounds to treat asthma reactions caused by allergens.
摘要翻译：公开了被羧酸官能团或生物疏水性极性官能团取代的氨基三唑化合物。具有羧酸酯部分或羧酸酯生物电子抑制剂的化合物抑制酸性哺乳动物几丁质酶。还提供了使用该化合物治疗由变应原引起的哮喘反应的方法。

27. 发明授权

US09440953B2 Substituted amino triazoles useful as acidic mammalian chitinase inhibitors 有权
标题翻译：用作酸性哺乳动物几丁质酶抑制剂的取代的氨基三唑
公开(公告)号：US09440953B2
公开(公告)日：2016-09-13
申请号：US14959369
申请日：2015-12-04
申请人： OncoArendi Therapeutics Sp z o.o.
发明人： Adam Golebiowski , Robert Koralewski , Wojciech J. Czestkowski , Krzysztof Matyszewski , Sylwia Olejniczak , Jacek Olczak , Paul Beckett
IPC分类号： C07D401/04 , A61K31/4196
CPC分类号： C07D401/04 , C07D401/14 , G01N33/573 , G01N2333/942 , G01N2500/04 , G01N2800/122 , G01N2800/52
摘要： Disclosed are amino triazole compounds substituted by a carboxylate functional group or an bioisosteric polar functional group. Compounds having the carboxylate moiety or carboxylate bioisostere inhibit acidic mammalian chitinase. Also provided are methods of using the compounds to treat asthma reactions caused by allergens.
摘要翻译：公开了被羧酸官能团或生物疏水性极性官能团取代的氨基三唑化合物。具有羧酸酯部分或羧酸酯生物电子抑制剂的化合物抑制酸性哺乳动物几丁质酶。还提供了使用该化合物治疗由变应原引起的哮喘反应的方法。

28. 发明申请

US20150174576A1 Microfluidic Device for Droplet Generation 审中-公开
标题翻译：用于产生液滴的微流体装置
公开(公告)号：US20150174576A1
公开(公告)日：2015-06-25
申请号：US14408800
申请日：2013-06-25
申请人： Imperial Innovations Limited , Cambridge Enterprise Limited
发明人： Liisa Darnell Van Vilet , Joshua Benno Edel , Andrew James Demello , Xize Niu , Sean Richard Anthony Devenish , Florian Hollfelder , Fabrice Matthieu Gielen
IPC分类号： B01L3/00 , B01L3/02 , G01N33/573
CPC分类号： B01L3/502784 , B01L3/0241 , B01L3/502715 , B01L2200/027 , B01L2200/0673 , B01L2300/0816 , B01L2300/0838 , B01L2400/0487 , G01N33/573 , G01N35/08 , G01N2035/1048 , G01N2333/942 , Y10T436/2575
摘要： The invention relates to a microfluidic system for generating droplets, the controlled merging of two or more droplets, and the analysis of droplets.
摘要翻译：本发明涉及用于产生液滴的微流体系统，两个或多个液滴的受控合并以及液滴的分析。

29. 发明申请

US20130323768A1 CULTURE SUBSTRATE COMPRISING CELLULOSE GEL, SOLID MEDIUM USING SAME, AND CELLULASE ACTIVITY ASSAY METHOD USING MEDIUM 有权
标题翻译：包含纤维素凝胶的培养基底，使用其的固体培养基和使用培养基的纤维素酶活性测定方法
公开(公告)号：US20130323768A1
公开(公告)日：2013-12-05
申请号：US13881756
申请日：2011-10-24
申请人： Kazunori Nagaki , Yasunori Kurosawa , Takuo Shiraishi , Shigeru Deguchi , Mikiko Tsudome
发明人： Kazunori Nagaki , Yasunori Kurosawa , Takuo Shiraishi , Shigeru Deguchi , Mikiko Tsudome
IPC分类号： C12Q1/34 , C12N1/22
CPC分类号： C12Q1/34 , C12N1/22 , C12N2533/78 , C12Q1/045 , G01N2333/942
摘要： A cellulose gel medium having good visibility of microbial colonies, a cellulose gel culture substrate for manufacturing the cellulose gel medium, a method for manufacturing the cellulose gel culture substrate, a method for screening cellulase-producing microorganisms or cellulase activity with greater efficiency and rapidity, and a culture substrate, which includes a cellulose gel containing cellulose and water as medium-solidifying components, the cellulose has the viscosity of 12 to 35 mPa·S as measured at 26° C. with a solution prepared by dissolving the cellulose at a concentration of 2.5 mg/mL in dimethylacetamide containing 8% (W/V) lithium chloride.
摘要翻译：具有微生物菌落的良好可见性的纤维素凝胶介质，用于制造纤维素凝胶介质的纤维素凝胶培养基质，纤维素凝胶培养基质的制造方法，以及更高的效率和快速地筛选纤维素酶生产微生物或纤维素酶活性的方法，和含有纤维素和水作为中固化成分的纤维素凝胶的培养基材，纤维素在26℃下测定的粘度为12〜35mPa·S，通过以浓度溶解纤维素制备的溶液在含有8％（W / V）氯化锂的二甲基乙酰胺中的浓度为2.5mg / mL。

30. 发明申请

US20100240082A1 METHODS FOR DETECTING AND MEASURING POLYSACCHARIDE-HYDROLYZING ENZYMES 审中-公开
标题翻译：检测和测量多糖 - 水解酶的方法
公开(公告)号：US20100240082A1
公开(公告)日：2010-09-23
申请号：US12726424
申请日：2010-03-18
申请人： Tricia Moser , Shib Sankar Basu , Rogerio T.N. Prata , Hangsik Moon , Xuejun Zhong , Myoung Kim
发明人： Tricia Moser , Shib Sankar Basu , Rogerio T.N. Prata , Hangsik Moon , Xuejun Zhong , Myoung Kim
IPC分类号： C12Q1/40 , C12Q1/34
CPC分类号： C12Q1/34 , C12Q1/40 , G01N2333/924 , G01N2333/928 , G01N2333/942 , G01N2333/944
摘要： Methods are disclosed for detecting and measuring polysaccharide-hydrolyzing enzyme activity or concentration by partial hydrolysis using a pre-determined, yet short, incubation time and a pre-determined temperature. The resulting reaction mixture has unique chemical (i.e., reaction products) and physical (i.e., viscosity) properties that can be used to detect or measure the polysaccharide-hydrolyzing enzyme activity or concentration.
摘要翻译：公开了通过使用预定的但短的孵育时间和预定温度通过部分水解来检测和测量多糖水解酶活性或浓度的方法。所得反应混合物具有独特的化学（即反应产物）和物理（即粘度）性质，可用于检测或测量多糖水解酶活性或浓度。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式