专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

21. 发明授权

US07790184B2 Cytochrome c synthesis inhibitors 有权
标题翻译：细胞色素c合成抑制剂
公开(公告)号：US07790184B2
公开(公告)日：2010-09-07
申请号：US11831273
申请日：2007-07-31
申请人： Robert Kranz
发明人： Robert Kranz
IPC分类号： A61K39/02
CPC分类号： G01N33/573 , C12Q1/025 , G01N2333/80
摘要： The invention provides methods for identifying a compound that inhibits cytochrome c synthesis. This invention further provides a method for the high throughput screening of compounds that inhibit cytochrome c synthesis.
摘要翻译：本发明提供了鉴定抑制细胞色素c合成的化合物的方法。本发明还提供了抑制细胞色素c合成的化合物的高通量筛选的方法。

22. 发明申请

US20090047655A1 Method Of Screening Candidate Drug 审中-公开
标题翻译：筛选候选药物的方法
公开(公告)号：US20090047655A1
公开(公告)日：2009-02-19
申请号：US11667855
申请日：2005-11-16
申请人： Chise Mukaidani , Katsutoshi Yoshizato , Yasufumi Nishikura , Toru Horie , Hiroshi Nakazawa , Hiroshi Kanamaru , Chiaki Ueda , Chiemi Hine
发明人： Chise Mukaidani , Katsutoshi Yoshizato , Yasufumi Nishikura , Toru Horie , Hiroshi Nakazawa , Hiroshi Kanamaru , Chiaki Ueda , Chiemi Hine
IPC分类号： C12Q1/00
CPC分类号： G01N33/5088 , G01N33/5067 , G01N2333/80
摘要： It is intended to provide a method of screening a candidate drug mainly metabolized by a drug metabolizing enzyme, CYP2D6, CYP2C9 or CYP2C19 with the use of a chimeric mouse carrying human hepatocytes transplanted thereinto, whereby it is determined whether or not the candidate drug is metabolized in a subject deficient in CYP2D6, CYP2C9 or CYP2C19. It is also intended to provide a method of determining whether or not a candidate drug induces or inhibits the activity of any of drug metabolizing enzymes, CYP1A2, CYP2C9, CYP2C19, CYP2D6 and CYP3A4 with the use of a chimeric mouse carrying human hepatocytes transplanted thereinto.
摘要翻译：本发明提供一种使用携带人肝细胞的嵌合小鼠筛选主要由药物代谢酶CYP2D6，CYP2C9或CYP2C19代谢的候选药物的方法，由此确定候选药物是否被代谢在CYP2D6，CYP2C9或CYP2C19缺乏的受试者中。还旨在提供一种使用携带人肝细胞的嵌合小鼠来确定候选药物是否诱导或抑制任何药物代谢酶CYP1A2，CYP2C9，CYP2C19，CYP2D6和CYP3A4的活性的方法。

23. 发明申请

US20070243577A1 Method for the detection of apoptosis by determining apoptosis-specific markers released into an extracellular medium through cellular release mechanisms 审中-公开
标题翻译：通过测定通过细胞释放机制释放到细胞外培养基中的凋亡特异性标记来检测凋亡的方法
公开(公告)号：US20070243577A1
公开(公告)日：2007-10-18
申请号：US11598199
申请日：2006-11-13
申请人： Marek Los , Andrea Renz , Klaus Schulze-Osthoff , Wolfgang Berdel
发明人： Marek Los , Andrea Renz , Klaus Schulze-Osthoff , Wolfgang Berdel
IPC分类号： C12Q1/18 , C12N5/00
CPC分类号： C12N9/0006 , A61K38/00 , C07K14/80 , G01N33/573 , G01N33/6896 , G01N2333/80 , G01N2333/904
摘要： A method for the detection of apoptosis by determining presence, amount, or activity of apoptosis-specific markers released from at least one cell undergoing apoptosis into an extracellular medium through cellular release mechanisms is disclosed. The extracellular medium includes body fluids, in particular urine, inflammatory fluid, serum, liquor, or cell culture medium, wherein samples are preferably taken from humans or animals. The method can be used for the diagnosis and/or for therapy control of diseases and/or processes associated with increased apoptosis. It can also be used for therapy control of diseases associated with decreased apoptosis. Additionally, a method is disclosed for the identification of apoptosis-modulating substances, characterized in that apoptosis-specific markers released from a cell culture sample into an extracellular cell culture medium through cellular release mechanisms are determined. The invention also concerns the use of an apoptosis detection kit for determining extracellular apoptosis-specific markers. It further relates to the use of cytochrome c and/or peptides derived thereof as a medicament, pharmaceutical compositions comprising cytochrome c and/or peptides derived thereof, and optionally a pharmaceutically acceptable carrier, and the use of cytochrome c and/or peptides derived thereof, for the preparation of a medicament for the treatment of diseases with inflammatory manifestation.
摘要翻译：公开了一种通过将细胞释放机制从至少一个经历细胞凋亡的细胞释放的细胞凋亡特异性标记物的存在，量或活性测定来检测细胞凋亡的方法。细胞外培养基包括体液，特别是尿液，炎性液体，血清，液体或细胞培养基，其中样品优选取自人或动物。该方法可用于与增加的凋亡相关的疾病和/或过程的诊断和/或治疗控制。它也可用于与凋亡减少相关的疾病的治疗控制。此外，公开了用于鉴定凋亡调节物质的方法，其特征在于确定通过细胞释放机制从细胞培养物样品释放到细胞外细胞培养基中的凋亡特异性标记。本发明还涉及凋亡检测试剂盒用于测定细胞外凋亡特异性标记物的用途。它还涉及使用其衍生的细胞色素c和/或肽作为药物，包含衍生自其的细胞色素c和/或肽的药物组合物，以及任选的药学上可接受的载体，以及衍生自其的细胞色素c和/或肽的用途，用于制备用于治疗具有炎性表现的疾病的药物。

24. 发明申请

US20070154971A1 AGENTS THAT BIND TO AND INHIBIT HUMAN CYTOCHROME P450 2C19 有权
标题翻译：与人体细胞色素P450 2C19相结合的药剂
公开(公告)号：US20070154971A1
公开(公告)日：2007-07-05
申请号：US11683229
申请日：2007-03-07
申请人： Harry Gelboin , Kristopher Krausz , Frank Gonzalez
发明人： Harry Gelboin , Kristopher Krausz , Frank Gonzalez
IPC分类号： G01N33/53 , C12Q1/26
CPC分类号： G01N33/5735 , G01N33/573 , G01N2333/80 , G01N2333/90245 , Y10T436/105831
摘要： The invention provides monoclonal antibodies and other binding agents to human cytochrome P450 2C19 having advantageous properties, including capacity substantially to inhibit enzyme activity of human cytochrome P450 2C19 and lack of specific binding to other human cytochrome P450s. The binding agents of the invention are useful inter alia in methods for screening drugs for metabolism by cytochrome P450 2C19, and in methods of measuring P450 2C19 levels in individuals relative to P450 2C19 levels in a control population.
摘要翻译：本发明提供了具有有利性质的人细胞色素P450 2C19的单克隆抗体和其他结合剂，其包括基本上抑制人细胞色素P450 2C19的酶活性和缺乏与其它人细胞色素P450的特异性结合的能力。本发明的结合剂特别可用于筛选细胞色素P450 2C19代谢药物的方法，以及测量个体相对于对照群体中P450 2C19水平的P450 2C19水平的方法。

25. 发明申请

US20070092926A1 Analysis of protein isoforms using unique tryptic peptides by mass spectrometry and immunochemistry 审中-公开
标题翻译：通过质谱和免疫化学分析使用独特的胰蛋白酶肽的蛋白质异构体
公开(公告)号：US20070092926A1
公开(公告)日：2007-04-26
申请号：US11581013
申请日：2006-10-13
申请人： Michail Alterman , Boris Kornilayev
发明人： Michail Alterman , Boris Kornilayev
IPC分类号： C12Q1/37
CPC分类号： G01N33/6851 , C07K16/40 , C07K2317/76 , G01N2333/80
摘要： A method for qualitatively and quantitatively detecting a protein isoform (p450 isozyme) in a sample using MALDI-TOF mass spectrometry or immunochemistry using a unique proteolytic peptide for the isoform. Relative and absolute quantitation can be performed using calibration curves with P450 isozyme-specific peptide standards.
摘要翻译：使用MALDI-TOF质谱或使用独特的蛋白水解肽为同种型免疫化学定性和定量检测样品中的蛋白质同种型（p450同功酶）的方法。可以使用具有P450同功酶特异性肽标准品的校准曲线进行相对和绝对定量。

26. 发明授权

US06902918B1 Oxygenase enzymes and screening method 失效
标题翻译：加氧酶和筛选方法
公开(公告)号：US06902918B1
公开(公告)日：2005-06-07
申请号：US09246451
申请日：1999-02-09
申请人： Frances H. Arnold , Hyun Joo , Zhanglin Lin
发明人： Frances H. Arnold , Hyun Joo , Zhanglin Lin
IPC分类号： C12N9/02 , C12N9/08 , C12N15/10 , C12N15/67 , C12N15/81 , C12Q1/26 , C12Q1/28 , C12N9/00 , C07H21/04 , C12N1/20 , C12N15/00
CPC分类号： C12N9/0071 , C07K2319/02 , C12N9/0065 , C12N15/102 , C12N15/1034 , C12N15/1058 , C12N15/1086 , C12N15/67 , C12N15/815 , C12Q1/26 , C12Q1/28 , G01N2333/795 , G01N2333/80 , G01N2333/90245
摘要： A method for detecting the presence of an oxygenated compound which is produced when a substrate is reacted with an oxygenase for the substrate. The method involves reacting a coupling enzyme with the oxygenated compound to form a polymeric oxygenated compound which is fluorescent or luminescent. Measurement of the fluorescence or luminescence of the polymeric oxygenated compound provides indirect detection of the oxygenated compound produced by reaction of the oxygenase with the substrate. The method is carried out in a whole cell environment wherein the cell is transformed to express both the oxygen a set being screened and the coupling enzyme. The method can be used to measure the activity of monooxygenases and dioxygenases on aromatic substrates. The method is amenable to large scale screening of enzyme mutants to isolate those with maximum oxygenase activity.
摘要翻译：用于检测当底物与用于底物的加氧酶反应时产生的含氧化合物的存在的方法。该方法包括使偶联酶与含氧化合物反应以形成荧光或发光的聚合的氧化化合物。聚合物氧化化合物的荧光或发光的测量提供了通过加氧酶与底物的反应产生的氧合化合物的间接检测。该方法在整个细胞环境中进行，其中细胞被转化以表达被筛选的一组氧和偶联酶。该方法可用于测量单加氧酶和双加氧酶在芳香族底物上的活性。该方法适用于大规模筛选酶突变体以分离具有最大加氧酶活性的那些。

27. 发明申请

US20040077080A1 Compositions and methods for induction of proteins involved in xenobiotic metabolism 审中-公开
标题翻译：用于诱导异种生物代谢的蛋白质的组合物和方法
公开(公告)号：US20040077080A1
公开(公告)日：2004-04-22
申请号：US10642322
申请日：2003-08-15
发明人： Judy Raucy
IPC分类号： C12Q001/68 , C12N009/10
CPC分类号： G01N33/5044 , C12N15/1034 , C12N15/63 , C12N2503/00 , C12N2510/00 , C12Q1/26 , C12Q1/48 , G01N33/5008 , G01N33/5023 , G01N33/5067 , G01N2333/795 , G01N2333/80 , G01N2333/90245
摘要： The present invention provides improved cells and methods for identifying compounds that alter protein expression, such as xenobiotics, endobiotics, chemicals or drugs. The invention provides other benefits as well. One aspect of the present invention is a cell that includes a first nucleic acid molecule that includes: a promoter or enhancer operable for a nucleic acid molecule encoding a human or non-human protein involved in drug metabolism (such as an enzyme or transporter) and a reporter gene; and a second nucleic acid encoding a human or non-human intracellular receptor or transcription factor; so that when the intracellular receptor or transcription factor is bound with a compound, the intracellular receptor, transporter or transcription factor can operably bind with the promoter or enhancer resulting in the expression of said reporter gene. Another aspect of the present invention is a method for evaluating compounds for the property of inducing the expression of a gene encoding a protein involved in drug metabolism, including; providing a test compound; contacting the test compound with a cell of the present invention; and detecting the expression of said reporter gene. This method can be a high throughput method.
摘要翻译：本发明提供改进的细胞和用于鉴定改变蛋白质表达的化合物的方法，例如异生物，内生生物，化学物质或药物。本发明还提供了其它益处。本发明的一个方面是包含第一核酸分子的细胞，所述第一核酸分子包括：可用于编码参与药物代谢的人或非人蛋白质的核酸分子的启动子或增强子（例如酶或转运蛋白）和记者基因; 和编码人或非人细胞内受体或转录因子的第二核酸; 使得当细胞内受体或转录因子与化合物结合时，细胞内受体，转运蛋白或转录因子可以与启动子或增强子可操作地结合，导致所述报告基因的表达。本发明的另一方面是评价化合物诱导编码参与药物代谢的蛋白质的表达的性质的方法，包括：提供试验化合物; 使测试化合物与本发明的细胞接触; 并检测所述报告基因的表达。该方法可以是高通量的方法。

28. 发明授权

US06643591B1 Use of computational and experimental data to model organic compound reactivity in cytochrome P450 mediated reactions and to optimize the design of pharmaceuticals 有权
标题翻译：使用计算和实验数据来模拟细胞色素P450介导的反应中的有机化合物反应性并优化药物的设计
公开(公告)号：US06643591B1
公开(公告)日：2003-11-04
申请号：US09368511
申请日：1999-08-05
申请人： Kenneth R. Korzekwa , Jeffrey P. Jones , Lee Ann Higgins
发明人： Kenneth R. Korzekwa , Jeffrey P. Jones , Lee Ann Higgins
IPC分类号： G06N700
CPC分类号： G01N33/6803 , C12Q1/26 , G01N2333/795 , G01N2333/80 , G01N2333/90245 , G06F19/12 , G06F19/16
摘要： The present invention is directed to the use of computational and other experimental data in the design of new pharmaceuticals, and in predicting the metabolism and toxicological profiles thereof. Computational and other information is used to further understand drug metabolism and toxicology, particulary in relation to monooxygenase enzymes, such as those of the CYP system, that are involved in drug metabolism. Information derived according to the practice of the invention is useful in determining the clearance or half-life of drugs, and the nature and toxicity of byproducts resulting from their metabolism. The invention provides novel and powerful new approaches to drug design.
摘要翻译：本发明涉及计算和其他实验数据在新药物设计中的应用，以及在预测其新陈代谢和毒理学特征。计算和其他信息用于进一步了解药物代谢和毒理学，特别是与单加氧酶相关的，例如参与药物代谢的CYP系统的那些。根据本发明的实践得到的信息可用于确定药物的清除率或半衰期，以及由其代谢产生的副产物的性质和毒性。本发明提供了新的和强大的药物设计新方法。

29. 发明授权

US06632630B2 Monooxygenase assays 失效
标题翻译：单加氧酶测定
公开(公告)号：US06632630B2
公开(公告)日：2003-10-14
申请号：US09820289
申请日：2001-03-28
申请人： Sharat Singh , Ahmed Chenna , Hossein Salimi-Moosari , Ian Gibbons
发明人： Sharat Singh , Ahmed Chenna , Hossein Salimi-Moosari , Ian Gibbons
IPC分类号： C12Q126
CPC分类号： C12Q1/26 , G01N2333/795 , G01N2333/80 , G01N2333/90245 , Y10S435/968
摘要： Cytochrome P-450 assay methods and kits for the methods are provided employing a cytochrome P-450 enzyme, substrates characterized by having an oxidizable methylene group oxidized to an aldehyde and a fluorescent hydrazine. A fluorescent hydrazine is added to the reaction mixture and the resulting hydrazone analyzed by capillary electrophoresis. The method finds use in evaluating compounds for enzyme modulating activity.
摘要翻译：使用细胞色素P-450酶提供细胞色素P-450测定方法和用于该方法的试剂盒，其特征在于具有氧化成醛的可氧化亚甲基和荧光肼。向反应混合物中加入荧光肼，通过毛细管电泳分析得到的腙。该方法可用于评价化合物的酶调节活性

30. 发明授权

US5213971A Process for inducing cytochrome P-450 enzymes in Streptomyces bacteria and determining the mutagenicity of chemicals 失效
标题翻译：在链霉菌中诱导细胞色素P-450酶的方法，并测定化学物质的致突变性
公开(公告)号：US5213971A
公开(公告)日：1993-05-25
申请号：US751046
申请日：1991-08-28
申请人： Daniel A. Kunz , Fateme S. Sariaslani
发明人： Daniel A. Kunz , Fateme S. Sariaslani
IPC分类号： C12N9/02 , C12N15/53 , C12N15/76 , C12P21/04 , C12Q1/02 , C12Q1/10 , C12Q1/26
CPC分类号： C12N9/0077 , C12N15/76 , C12Q1/025 , C12Q1/10 , C12Q1/26 , G01N2333/255 , G01N2333/36 , G01N2333/795 , G01N2333/80 , G01N2333/902 , G01N2333/90245 , Y10S435/886 , Y10S435/897
摘要： A process for inducing cytochrome P-450 enzyme production in bacteria of the genus Streptomyces using inducers such as soybean flour, genistein or genistin is described. Uses for the cytochrome P-450 enzymes produced are also discussed as is a process for using genetically engineered Streptomyces to determine the mutagenicity of chemicals.
摘要翻译：描述了使用诱导剂如大豆粉，染料木素或染料木素在链霉菌属细菌中诱导细胞色素P-450酶产生的方法。还讨论了生产的细胞色素P-450酶的用途，是使用遗传工程化链霉菌来确定化学品的致突变性的方法。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式