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21. 发明申请

WO2012038450A1 MICROWAVE-DRIVEN RNA POLYMERIZATION BY RNA POLYMERASES OF CALICIVIRUSES 审中-公开
标题翻译：通过RNA聚合酶进行微波驱动的RNA聚合
公开(公告)号：WO2012038450A1
公开(公告)日：2012-03-29
申请号：PCT/EP2011/066362
申请日：2011-09-20
申请人： RIBOXX GMBH , ROHAYEM, Jacques
发明人： ROHAYEM, Jacques
IPC分类号： C12Q1/68
CPC分类号： C12P19/34 , C12Q1/6853 , C12Q2525/101 , C12Q2523/313 , C12Q2521/119
摘要： The present invention relates to a method for polymerising a complementary RNA strand on a single-stranded polynucleotide template comprising the step of irradiating a composition containing said template and an RNA polymerase of a virus of the Caliciviridae family under RNA polymerisation conditions in the presence or absence of a primer hybridised to the template, with an effective amount of microwave energy. Further subject matter of the invention relates to a method for transferring one or more ribonucleotides to the 3' end of a single-stranded polynucleotide template comprising the step of irradiating a composition containing an RNA polymerase of a virus of the Caliciviridae family in the presence of rATP or rGTP or rUTP or rCTP or a modified or labelled analogue thereof with an effective amount of microwave energy.
摘要翻译：本发明涉及用于在单链多核苷酸模板上聚合互补RNA链的方法，包括在RNA聚合条件下，存在或不存在下照射含有所述模板的组合物和Caliciviridae家族病毒的RNA聚合酶的步骤的引物与模板杂交，具有有效量的微波能量。本发明的另外的主题涉及用于将一个或多个核糖核苷酸转移到单链多核苷酸模板的3'末端的方法，包括以下步骤：在含有Caliciviridae家族病毒的RNA聚合酶的组合物的存在下， rATP或rGTP或rUTP或rCTP或其具有有效量的微波能量的经修饰或标记的类似物。

22. 发明申请

WO2011048198A2 METHOD AND RNA REACTOR FOR EXPONENTIAL AMPLIFICATION OF RNA 审中-公开
标题翻译： RNA的免疫扩增方法和RNA反应器
公开(公告)号：WO2011048198A2
公开(公告)日：2011-04-28
申请号：PCT/EP2010/065912
申请日：2010-10-21
申请人： RIBOXX GMBH , ROHAYEM, Jacques
发明人： ROHAYEM, Jacques
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q2521/119
摘要： The present invention relates to a method for exponential amplification of RNA using a primer independent RNA-dependent RNA polymerase (RdRp) wherein reactants are premixed cycle and then transferred into the reaction chamber in which the steps of polymerisation of the complementary strand and separation of the resulting double-stranded RNA occur. The invention also relates to a RNA reactor for carrying out the exponential RNA amplification.
摘要翻译：本发明涉及使用独立于引物的RNA依赖性RNA聚合酶（RdRp）对RNA进行指数扩增的方法，其中反应物是预混合循环，然后转移到反应室中，其中互补链聚合的步骤和产生双链RNA。本发明还涉及用于进行指数RNA扩增的RNA反应器。

23. 发明申请

WO2010055134A1 RNA DETECTION METHOD 审中-公开
标题翻译： RNA检测方法
公开(公告)号：WO2010055134A1
公开(公告)日：2010-05-20
申请号：PCT/EP2009/065131
申请日：2009-11-13
申请人： RIBOXX GMBH , ROHAYEM, Jacques
发明人： ROHAYEM, Jacques
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6844 , C12Q2521/119 , C12Q2531/119
摘要： The present invention relates to methods for the detection of target RNA sequences and to RNA amplification methods making use of strand displacement techniques employing RNA-dependent RNA polymerases having RNA-oligonucleotide duplex separation activity and being capable of de novo RNA synthesis in the absence of a primer. The present invention further relates to kits for carrying out such methods.
摘要翻译：本发明涉及用于检测靶RNA序列的方法和使用使用具有RNA-寡核苷酸双链体分离活性的RNA依赖性RNA聚合酶的链置换技术的RNA扩增方法，并且能够在没有底漆。本发明还涉及用于实施这些方法的试剂盒。

24. 发明申请

WO2022013324A2 POST-EXPOSURE VACCINATION AGAINST VIRAL RESPIRATORY INFECTIONS 审中-公开
公开(公告)号：WO2022013324A2
公开(公告)日：2022-01-20
申请号：PCT/EP2021/069673
申请日：2021-07-14
申请人： RIBOXX GMBH , TRENZYME GMBH , TER MEULEN, Jan
发明人： ROHAYEM, Jacques , HORLACHER, Reinhold
IPC分类号： A61K39/12 , A61P31/14 , A61P31/16
摘要： The present invention relates to pharmaceutical compositions, in particular vaccine compositions, for preventing or at least reducing the severity of, respectively, viral respiratory infections through application of said composition to a human subject post-exposure or at least presumed post-exposure of said subject to a virus causing said viral respiratory infections or pre-exposure of said subject to said virus. More particularly, in specific embodiments, the invention provides pharmaceutical compositions as such comprising at least one antigenic component of the infectious virus and a TLR-3 agonist. The invention also relates to methods of treatment and/or prevention of said viral respiratory infections through administration of the composition to the human subject post exposure or at least presumed post-exposure of said subject to the infectious virus or pre-exposure of said subject to said virus.

25. 发明申请

WO2016112963A1 DELIVERY OF BIOMOLECULES INTO CELLS 审中-公开
标题翻译：生物分子进入细胞
公开(公告)号：WO2016112963A1
公开(公告)日：2016-07-21
申请号：PCT/EP2015/050519
申请日：2015-01-13
申请人： RIBOXX GMBH
发明人： ROHAYEM, Jacques
IPC分类号： C12N15/87 , C12N15/115
CPC分类号： C12N15/87 , C12N15/111 , C12N2310/151 , C12N2310/18 , C12N2320/32
摘要： The present invention generally relates to compositions or complexes, respectively, containing a biologically active substance or biomolecule and at least one nucleotide, oligonucleotide and polynucleotide forming G-quadruplexes and/or intramolecular and/or intermolecular structures and/or suprastructures under appropriate conditions, compositions containing the same, methods for their production and their use as delivery reagents and/or transfection reagents in vitro and in vivo . The present invention also relates to specific embodiments of G-quadruplex forming oligonucleotides or polynucleotides, respectively, per se .
摘要翻译：本发明通常涉及分别含有生物活性物质或生物分子的组合物或复合物，以及在合适条件下形成G-四链体和/或分子内和/或分子间结构和/或超结构的至少一种核苷酸，寡核苷酸和多核苷酸，组合物含有相同的生产方法及其在体外和体内作为递送试剂和/或转染试剂的用途。本发明还分别涉及G-四链体形成寡核苷酸或多核苷酸的具体实施方案。

26. 发明申请

WO2015144736A1 DOUBLE-STRANDED RNA CONJUGATES AND THEIR USE 审中-公开
标题翻译：双链RNA连接及其使用
公开(公告)号：WO2015144736A1
公开(公告)日：2015-10-01
申请号：PCT/EP2015/056318
申请日：2015-03-24
申请人： RIBOXX GMBH
发明人： ROHAYEM, Jacques , NAUMANN, Kai
IPC分类号： C12N15/117 , A61K31/713 , A61K47/48
CPC分类号： C12N15/117 , A61K39/00 , A61K39/39 , A61K47/54 , A61K47/545 , A61K47/551 , A61K47/554 , A61K47/60 , A61K47/6807 , A61K2039/55583 , C12N2310/17 , C12N2310/351 , C12N2310/3513 , C12N2310/3515 , C12N2320/32
摘要： The present invention relates to double-stranded ribonucleic acids (dsRNA) of at least 45 bp, preferably of at least 50 bp, which dsRNA comprises at least one 5'- triphosphate group and further comprises at least one chemical modification at a 5' end, at a 3' end and/or at a non-terminal nucleotide. The invention further provides pharmaceutical compositions containing such modified dsRNAs, methods for their production, and to their use in medicine, in particular for immunostimulation and treatment as well as prevention of infectious, autoimmune, degenerative, cancer and tumor diseases.
摘要翻译：本发明涉及至少45bp，优选至少50bp的双链核糖核酸（dsRNA），该dsRNA包含至少一个5'-三磷酸基团，并且还包含在5'末端的至少一个化学修饰在3'末端和/或非末端核苷酸处。本发明还提供含有这种修饰的dsRNA的药物组合物，其生产方法及其在医药中的用途，特别是用于免疫刺激和治疗，以及预防感染性，自身免疫性，退行性，癌症和肿瘤疾病。

27. 发明申请

WO2015091578A1 DOUBLE-STRANDED POLYC:POLY(G/I) RNA FOR IMMUNOSTIMULATION AND CANCER TREATMENT 审中-公开
标题翻译：双链多聚（POLYC）：聚（G / I）RNA用于免疫和癌症治疗
公开(公告)号：WO2015091578A1
公开(公告)日：2015-06-25
申请号：PCT/EP2014/078100
申请日：2014-12-16
申请人： RIBOXX GMBH
发明人： ROHAYEM, Jacques
IPC分类号： C12N15/117 , A61K31/713
CPC分类号： C12N15/117 , A61K39/39 , A61K45/06 , A61K2039/55561 , C12N2310/17 , C12N2310/331 , C12N2310/351 , C12N2310/53 , C12N2310/533 , C12N2330/50 , C12P19/34
摘要： The present invention relates to polyC:poly(G/l) dsRNAs for triggering innate immunity, in particular through toll-like receptor 3 (TLR-3) and, optionally, RIG-I or RIG-I— like receptors (RLRs), as well as compositions and medicaments containing such dsRNAs, methods for their production and their use in medicine, especially immunostimulation and prevention and/or therapy of infections and tumor diseases.
摘要翻译：本发明涉及用于触发先天免疫的polyC：poly（G / l）dsRNA，特别是通过toll样受体3（TLR-3）和任选的RIG-1或RIG-I-样受体（RLR），以及含有这种dsRNA的组合物和药物，其生产方法及其在医学中的应用，特别是免疫刺激和预防和/或治疗感染和肿瘤疾病。

28. 发明申请

WO2012107538A1 METHOD FOR LABELLING DOUBLE-STRANDED DNA OR DNA/RNA HYBRIDS 审中-公开
标题翻译：用于标记双链DNA或DNA / RNA混合物的方法
公开(公告)号：WO2012107538A1
公开(公告)日：2012-08-16
申请号：PCT/EP2012/052247
申请日：2012-02-09
申请人： RIBOXX GMBH , ROHAYEM, Jacques
发明人： ROHAYEM, Jacques
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6846 , C12N9/127 , C12Y207/07048 , C12Q2521/119 , C12Q2521/131
摘要： The present invention relates to a method for adding at least one ribonucleotide to the 3 ' - terminal deoxyribnucleotide of a polynucleotide. The method uses the terminal transferase activity of an RNA- dependent RNA polymerase such as from the calciviruses.
摘要翻译：本发明涉及向多核苷酸的3'端脱氧核糖核苷酸添加至少一种核糖核苷酸的方法。该方法使用RNA依赖性RNA聚合酶的末端转移酶活性，例如来自钙化病毒。

29. 发明申请

WO2012038448A1 METHOD FOR SYNTHESIZING RNA USING DNA TEMPLATE 审中-公开
标题翻译：使用DNA模板合成RNA的方法
公开(公告)号：WO2012038448A1
公开(公告)日：2012-03-29
申请号：PCT/EP2011/066360
申请日：2011-09-20
申请人： RIBOXX GMBH , ROHAYEM, Jacques
发明人： ROHAYEM, Jacques
IPC分类号： C12N15/10 , C12P19/34 , C12N9/12 , C12N9/22
CPC分类号： C12P19/34
摘要： The present invention relates to a method of RNA synthesis by RNA-dependent RNA polymerases (RdRp) displaying an RNA polymerase activity on single-stranded DNA templates and to a kit for carrying out the method. The RdRp showing DNA-dependent RNA polymerase activity has a "right hand conformation" and the amino acid sequence of said RdRp comprises a conserved arrangement of the following sequence motifs: a. XXDYS, b. GXPSG, c. YGDD, d. XXYGL, e. XXXXFLXRXX (with the following meanings: D: aspartate, Y: tyrosine, S: serine, G: glycine, P: proline, L: leucine, F: phenylalanine, R: arginine, X: any amino acid). This class of RdRp is exemplified by the RdRp enzymes of viruses of the Caliciviridae family. The present invention also relates to a method for transferring at least one ribonucleotide (rC, rA, rU or rG) to the 3'-end of a single-stranded DNA by using the RdRp of the invention.
摘要翻译：本发明涉及通过在单链DNA模板上显示RNA聚合酶活性的RNA依赖性RNA聚合酶（RdRp）和用于实施该方法的试剂盒来RNA合成的方法。显示DNA依赖性RNA聚合酶活性的RdRp具有“右手构象”，并且所述RdRp的氨基酸序列包含以下序列基序的保守排列：a。 XXDYS，b。 GXPSG，c。 YGDD，d。 XXYGL，e。 XXXXFLXRXX（具有以下含义：D：天冬氨酸，Y：酪氨酸，S：丝氨酸，G：甘氨酸，P：脯氨酸，L：亮氨酸，F：苯丙氨酸，R：精氨酸，X：任何氨基酸）。该类RdRp的例子是Caliciviridae家族病毒的RdRp酶。本发明还涉及通过使用本发明的RdRp将至少一种核糖核苷酸（rC，rA，rU或rG）转移到单链DNA的3'-末端的方法。

30. 发明申请

WO2011048198A3 METHOD AND RNA REACTOR FOR EXPONENTIAL AMPLIFICATION OF RNA 审中-公开
标题翻译：用于指数扩增RNA的方法和RNA反应器
公开(公告)号：WO2011048198A3
公开(公告)日：2011-12-08
申请号：PCT/EP2010065912
申请日：2010-10-21
申请人： RIBOXX GMBH , ROHAYEM JACQUES
发明人： ROHAYEM JACQUES
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q2521/119
摘要： The present invention relates to a method for exponential amplification of RNA using a primer independent RNA-dependent RNA polymerase (RdRp) wherein reactants are premixed cycle and then transferred into the reaction chamber in which the steps of polymerisation of the complementary strand and separation of the resulting double-stranded RNA occur. The invention also relates to a RNA reactor for carrying out the exponential RNA amplification.
摘要翻译：本发明涉及使用不依赖于引物的RNA依赖性RNA聚合酶（RdRp）对RNA进行指数扩增的方法，其中反应物是预混合循环，然后转移到反应室中，其中互补链聚合和分离产生双链RNA。本发明还涉及用于进行指数RNA扩增的RNA反应器。

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