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    • 22. 发明申请
    • METHOD FOR DETERMINING ACTIVITY OF CELL CYCLE REGULATORY FACTOR AND METHOD FOR DIAGNOSING CANCER USING THE SAME
    • 用于确定细胞周期调节因子的活性的方法和使用其诊断癌症的方法
    • US20070264624A1
    • 2007-11-15
    • US11747077
    • 2007-05-10
    • Hideki IshiharaTomokazu YoshidaMasatoshi YamasakiSachiyo Tada
    • Hideki IshiharaTomokazu YoshidaMasatoshi YamasakiSachiyo Tada
    • C12Q1/00
    • C07K16/40C12N9/1205C12Q1/48C12Q1/485G01N33/57407G01N33/57484G01N33/581G01N33/582G01N2333/9121
    • A method for determining the activity of a cell cycle regulatory factor comprising the steps of preparing a sample for measuring a cyclin-dependent kinase/cyclin complex from living cells; reacting adenosine 5′-O-(3-thiotriphosphate) (ATP-γ S) with a substrate for the cyclin-dependent kinase in presence of the sample in order to introduce a monothiophosphate group into a serine or threonine residue of the substrate; labeling the substrate by coupling a labeling fluorophore or a labeling enzyme with a sulfur atom of the introduced monothiophosphate group; measuring the amount of fluorescence from the labeling fluorophore labeling the substrate, or reacting the labeling enzyme labeling the substrate with a substance which generates an optically detectable product by reaction with the labeling enzyme and optically measuring the amount of the generated product; and calculating the activity of the cyclin-dependent kinase from the measured amount of fluorescence or the measured amount of the generated product with reference to a pre-produced reference curve.
    • 一种确定细胞周期调控因子活性的方法,包括以下步骤:制备用于从活细胞测量细胞周期蛋白依赖性激酶/细胞周期蛋白复合物的样品; 在样品存在下使腺苷5'-O-(3-硫代三磷酸)(ATP-γS)与用于细胞周期蛋白依赖性激酶的底物反应,以将一硫代磷酸酯基团引入底物的丝氨酸或苏氨酸残基中; 通过将标记的荧光团或标记酶与引入的一硫代磷酸酯基团的硫原子偶联来标记底物; 测量来自标记荧光团的荧光标记基质的荧光量,或使标记基质的标记酶与通过与标记酶反应产生可光学检测的产物并光学测量产生的产物的量的物质反应; 并且根据测量的荧光量或参考预先产生的参考曲线测量产生的产物的量计算细胞周期蛋白依赖性激酶的活性。
    • 23. 发明授权
    • Auto focus adjustment apparatus and image pickup apparatus
    • 自动对焦调整装置和摄像装置
    • US08952306B2
    • 2015-02-10
    • US12907278
    • 2010-10-19
    • Tomokazu Yoshida
    • Tomokazu Yoshida
    • G02B7/04G06M7/00G02B7/28
    • G02B7/28
    • An auto focus adjustment apparatus includes: a correction unit which corrects a defocus amount detected by a defocus amount detection unit, correspondingly to a focus area selected from a plurality of focus areas; a storage unit which stores a correction value to be used by the correction unit at a time of correcting the defocus amount; and a correction value changing unit configured to change the correction value, wherein the correction value changing unit is arranged to be capable of changing the correction value for a plurality of selected focus areas and at a plurality of zoom positions.
    • 自动对焦调整装置包括:校正单元,其对应于从多个焦点区域选择的对焦区域来校正由散焦量检测单元检测到的散焦量; 存储单元,其存储在校正所述散焦量时由所述校正单元使用的校正值; 以及校正值改变单元,被配置为改变校正值,其中校正值改变单元被布置为能够改变多个选择的聚焦区域和多个变焦位置处的校正值。
    • 25. 发明申请
    • EMULSIFIED COMPOSITION
    • 乳化组合物
    • US20120108661A1
    • 2012-05-03
    • US13382298
    • 2010-07-06
    • Masanori OritaTomokazu YoshidaChihiro Ueyama
    • Masanori OritaTomokazu YoshidaChihiro Ueyama
    • A61K8/68A61Q19/00A61K8/37
    • A61K8/68A61K8/06A61K8/342A61K8/345A61K8/37A61K8/375A61K8/41A61K8/60A61K8/86A61Q19/00
    • The emulsion composition of the present invention contains (A) 0.001 to 10 wt. % of an organic compound having two or more hydroxyl groups, an inorganic value of 220 to 450, and an organic value of 300 to 1,000; (B) 0.001 to 10 wt. % of an organic compound having one hydroxyl group, an inorganic value of 100 to 200, and an organic value of 280 to 700; (C) 0.001 to 10 wt. % of a compound represented by formula (2): wherein R1 is a C4 to C30 hydrocarbon group; Z is a methylene group, a methine group, or an oxygen atom; X1, X2, X3 is a hydrogen atom, a hydroxyl group, or an acetoxy group; X4 is a hydrogen atom, an acetyl group, or a glyceryl group; each of R2 and R3 a hydrogen atom, a hydroxyl group, a hydroxymethyl group, or an acetoxymethyl group; R4 is a C5 to C60 hydrocarbon group; and R5 is a hydrogen atom or a hydrocarbon group containing 1 to 30 carbon atoms in total; (D) 0.00012 to 10 wt. % of at least one compound selected from the group consisting of a nonionic surfactant having a polyoxyethylene group and an HLB of 10 or higher, an ionic surfactant, and a sphingosine salt; (E) 0.003 to 15 wt. % of at least one compound selected from the group consisting of a sugar alcohol selected from the group consisting of erythritol, threitol, xylitol, and mannitol, a disaccharide, and a trisaccharide; and (F) water.
    • 本发明的乳液组合物含有(A)0.001〜10重量% %的具有两个或更多个羟基的有机化合物,无机值为220至450,有机值为300至1,000; (B)0.001〜10重量% %的具有一个羟基的有机化合物,无机值为100至200,有机值为280至700; (C)0.001〜10重量% %的由式(2)表示的化合物:其中R1是C4-C330烃基; Z是亚甲基,次甲基或氧原子; X 1,X 2,X 3为氢原子,羟基或乙酰氧基; X4是氢原子,乙酰基或甘油基; R 2和R 3各自为氢原子,羟基,羟甲基或乙酰氧基甲基; R4为C5〜C60烃基; R5为氢原子或总共含有1〜30个碳原子的烃基; (D)0.00012〜10重量% %的选自由聚氧乙烯基和HLB为10以上的非离子表面活性剂,离子表面活性剂和鞘氨醇盐组成的组中的至少一种化合物; (E)0.003〜15重量% %选自选自赤藓糖醇,苏糖醇,木糖醇和甘露糖醇的糖醇,二糖和三糖中的至少一种化合物; 和(F)水。
    • 27. 发明申请
    • CAMERA AND PHOTOGRAPHIC LENS
    • 相机和摄影镜头
    • US20110052176A1
    • 2011-03-03
    • US12940959
    • 2010-11-05
    • Tomokazu Yoshida
    • Tomokazu Yoshida
    • G03B7/095G02B9/00
    • G03B7/095
    • A camera is provided which is adapted to receive a photographic lens having a diaphragm. The camera includes a detection unit configured to execute electric charge accumulation for detecting a defocus amount when the diaphragm is in a state having a first aperture value, and a controller configured to, when causing the diaphragm to perform an aperture reducing operation from the first aperture value to a second aperture value, cause the diaphragm to start the aperture reducing operation without waiting for completion of the electric charge accumulation for detecting the defocus amount by the detection unit.
    • 提供了适于接收具有隔膜的摄影镜头的照相机。 摄像机包括:检测单元,被配置为执行电荷累积,用于当所述隔膜处于具有第一孔径值的状态时检测散焦量;以及控制器,被配置为当使所述隔膜从所述第一孔径执行孔径减小操作时 值到第二光圈值,使得光阑开始光圈减小操作,而不用等待完成由检测单元检测散焦量的电荷累积。
    • 30. 发明申请
    • CAMERA AND PHOTOGRAPHIC LENS
    • 相机和摄影镜头
    • US20080199174A1
    • 2008-08-21
    • US12028231
    • 2008-02-08
    • Tomokazu Yoshida
    • Tomokazu Yoshida
    • G03B7/095
    • G03B7/095
    • A camera is provided which is adapted to receive a photographic lens having a diaphragm. The camera includes a detection unit configured to execute electric charge accumulation for detecting a defocus amount when the diaphragm is in a state having a first aperture value, and a controller configured to, when causing the diaphragm to perform an aperture reducing operation from the first aperture value to a second aperture value, cause the diaphragm to start the aperture reducing operation without waiting for completion of the electric charge accumulation for detecting the defocus amount by the detection unit.
    • 提供了适于接收具有隔膜的摄影镜头的照相机。 摄像机包括:检测单元,被配置为执行电荷累积,用于当所述隔膜处于具有第一孔径值的状态时检测散焦量;以及控制器,被配置为当使所述隔膜从所述第一孔径执行孔径减小操作时 值到第二光圈值,使得光阑开始光圈减小操作,而不用等待完成由检测单元检测散焦量的电荷累积。