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21. 发明申请

US20100330699A1 APPARATUS AND METHOD FOR THE DETECTION OF LIQUIDS OR SUBSTANCES FROM LIQUIDS, AND USE OF SAID APPARATUS 有权
标题翻译：用于检测液体中液体或物质的装置和方法以及使用装置
公开(公告)号：US20100330699A1
公开(公告)日：2010-12-30
申请号：US12867652
申请日：2009-02-09
申请人： Walter Gumbrecht , Peter Paulicka , Manfred Stanzel
发明人： Walter Gumbrecht , Peter Paulicka , Manfred Stanzel
IPC分类号： G01N33/566 , G01N33/50 , G01N27/00 , G01N15/04
CPC分类号： G01N27/27 , G01N15/04 , G01N27/30 , G01N27/40 , G01N33/50 , G01N33/566 , Y10T436/143333
摘要： The embodiments relate to an apparatus and a method for detecting liquids or substances from liquids in spatially separate reaction zones. The embodiments further relate to the use of the apparatus in a plug-in module or a chip card which can be used for rapid immunological tests, for example, with the help of a reading device. The liquids or substances from liquids are detected by means of a sensor array which includes at least two sensors and on which at least one diaphragm is arranged. Individual sensors are spatially separated from each other on a plane by means of walls. The diaphragm can be filled with liquid that is to be analyzed. Sealed reaction chambers are created when pressure is applied to the diaphragm. Pores in the diaphragm are completely closed in the zone of the walls while the pores are merely reduced in size and liquid can continue to be exchanged in zones directly above the sensors. No liquid can be exchanged between adjacent reaction chambers as long as the pressure is applied to and compresses the diaphragm. In the sealed reaction chambers, reactions can take place independently of reactions in adjacent reaction chamber. Reaction products or substances in the liquid can be measured with smaller measurement errors in sealed reaction chamber than when the liquid flows across the entire diaphragm.
摘要翻译：实施例涉及用于在空间上分离的反应区域中检测来自液体的液体或物质的装置和方法。实施例还涉及在插件模块或芯片卡中使用该装置，其可以用于例如借助于读取装置的快速免疫测试。通过包括至少两个传感器的传感器阵列检测来自液体的液体或物质，并且在该传感器阵列上布置有至少一个隔膜。单个传感器在空间上通过壁在空间上彼此分开。隔膜可以填充待分析的液体。当对隔膜施加压力时，产生密封的反应室。隔膜中的孔隙在壁的区域中完全封闭，而孔仅尺寸减小，并且液体可以在传感器正上方的区域中继续更换。只要施加压力并压缩隔膜，相邻反应室之间就不能更换液体。在密封反应室中，反应可以独立于相邻反应室中的反应进行。液体中的反应产物或物质可以在密封反应室中以比液体流过整个隔膜的更小的测量误差来测量。

22. 发明申请

US20100009864A1 Method for analysing amplified nucleic acids 有权
标题翻译：分析扩增核酸的方法
公开(公告)号：US20100009864A1
公开(公告)日：2010-01-14
申请号：US12308552
申请日：2007-05-31
申请人： Walter Gumbrecht , Peter Paulicka , Manfred Stanzel
发明人： Walter Gumbrecht , Peter Paulicka , Manfred Stanzel
IPC分类号： C40B30/04 , C12Q1/68 , C12M1/00
CPC分类号： C12Q1/6827 , B01L3/5027 , C12Q1/6846 , C12Q2565/629 , C12Q2527/125
摘要： An example embodiment of the present invention discloses a method for analyzing nucleic acids in a microfluidic device. The method includes the following steps: a) nucleic acids are amplified in a first chamber in the microfluidic device; b) the amplified nucleic acids are brought into contact with an additive including: i) monovalent cations and ii) an Mg2+ ion-binding agent, the additive being provided in a second chamber in the microfluidic device; and c) the amplified nucleic acids are hybridized on at least one probe oligonucleotide.
摘要翻译：本发明的示例性实施方案公开了一种用于分析微流体装置中的核酸的方法。该方法包括以下步骤：a）核酸在微流体装置的第一腔室中扩增; b）使扩增的核酸与添加剂接触，包括：i）一价阳离子和ii）Mg 2+离子结合剂，所述添加剂设置在微流体装置中的第二室中; 和c）扩增的核酸在至少一种探针寡核苷酸上杂交。

23. 发明授权

US07510839B2 Method for the combined isolation of magnet-beads from a liquid sample and subsequent thermocyclisation for the polymerase chain reaction (PCR) and associated arrangement 有权
标题翻译：用于从液体样品中组合分离磁珠的方法和用于聚合酶链式反应（PCR）和相关布置的随后的热循环
公开(公告)号：US07510839B2
公开(公告)日：2009-03-31
申请号：US11665376
申请日：2005-10-14
申请人： Walter Gumbrecht , Peter Paulicka , Manfred Stanzel
发明人： Walter Gumbrecht , Peter Paulicka , Manfred Stanzel
IPC分类号： C12Q1/68 , G01N33/557 , G01N33/553 , G01N33/536 , C12P19/34
CPC分类号： B01L7/52 , B01L3/502761 , B01L2200/0668 , B01L2300/0816 , B01L2300/0877 , B01L2300/1822 , B01L2400/0661 , G01N35/0098
摘要： Embodiments generally relate to a PCR wherein a magnetic field is focused in a localized area and also to the possibility of a cyclic transfer of heat. According to at least one embodiment of the invention, a magnetic field gradient is constructed along the direction of flow and the transfer of heat is carried out in a manner perpendicular to the direction of flow. Suitable things are provided therefore. The magnetic field is, in particular, focused by Mumetal® which is arranged on both sides of the flow channel and the temperature is controlled by way of Peltier elements, cooling bodies, and thermal coupling plates.
摘要翻译：实施方案通常涉及PCR，其中磁场聚焦在局部区域中，并且还涉及循环传递热的可能性。根据本发明的至少一个实施例，沿着流动方向构造磁场梯度，并且以垂直于流动方向的方式进行热传递。因此提供合适的东西。特别地，磁场被布置在流动通道两侧的Mumetal聚焦，并且温度通过珀耳帖元件，冷却体和热耦合板来控制。

24. 发明申请

US20070219366A1 Method and Assembly for Dna Isolation With Dry Reagents 有权
标题翻译：用干试剂进行Dna隔离的方法和装配
公开(公告)号：US20070219366A1
公开(公告)日：2007-09-20
申请号：US11587581
申请日：2005-04-28
申请人： Walter Gumbrecht , Peter Paulicka , Manfred Stanzel
发明人： Walter Gumbrecht , Peter Paulicka , Manfred Stanzel
IPC分类号： C12N15/10
CPC分类号： B01L3/502761 , B01J2219/00286 , B01J2219/00459 , B01J2219/005 , B01J2219/00648 , B01J2219/00722 , B01L7/52 , B01L2200/0668 , B01L2200/082 , B01L2200/10 , B01L2200/16 , B01L2300/0816 , B01L2400/0406 , B01L2400/0487 , C12N15/1013 , C12Q1/6806 , C12Q2563/143 , C12Q2531/113
摘要： A DNA isolation method by removal of constituents, interfering with a subsequent PCR is disclosed. According to an embodiment of the method, all substances and method steps are fully integrated into a closed unit (cartridge) for single use, which allows entry of a DNA-containing sample and DNA-binding substrates are used for isolating the released DNA. In particular, when the method is applied to DNA isolation from whole blood by disruption of white blood cells, the reagents, required for carrying out the cell disruption and other reactions, are stored in a form which is stable at room temperature. For disrupting the white blood cells and for DNA isolation, a dry stored lysis reagent is dissolved in an aqueous solution and brought into contact with the white blood cells. The corresponding assembly includes a unit, for housing DNA-containing biological containers and/or reagents, whereby at least one microchannel is provided to contain reagents, whereby the reagent is present in the microchannel as a dry mixture with a negligible vapour pressure, which forms a stable substance at room temperature.
摘要翻译：公开了通过去除组分，干扰后续PCR的DNA分离方法。根据该方法的一个实施方案，所有物质和方法步骤完全整合到用于单次使用的封闭单元（盒）中，其允许含DNA样品的进入和DNA结合底物用于分离释放的DNA。特别是，当将该方法应用于通过破坏白细胞从全血中分离DNA时，进行细胞破碎和其它反应所需的试剂以在室温下稳定的形式储存。为了破坏白细胞和进行DNA分离，将干燥储存的裂解试剂溶解在水溶液中并与白细胞接触。相应的组件包括用于容纳含有DNA的生物容器和/或试剂的单元，由此提供至少一个微通道以容纳试剂，由此所述试剂作为干燥的混合物存在于微通道中，所述干燥混合物具有可忽略的蒸气压，其形成在室温下稳定的物质。

25. 发明授权

US09091645B2 Apparatus and method for the detection of liquids or substances from liquids, and use of said apparatus 有权
标题翻译：用于检测来自液体的液体或物质的装置和方法以及所述装置的使用
公开(公告)号：US09091645B2
公开(公告)日：2015-07-28
申请号：US12867652
申请日：2009-02-09
申请人： Walter Gumbrecht , Peter Paulicka , Manfred Stanzel
发明人： Walter Gumbrecht , Peter Paulicka , Manfred Stanzel
IPC分类号： G01N33/566 , G01N15/04 , G01N27/00 , G01N33/50 , G01N27/40 , G01N27/30
CPC分类号： G01N27/27 , G01N15/04 , G01N27/30 , G01N27/40 , G01N33/50 , G01N33/566 , Y10T436/143333
摘要： The embodiments relate to an apparatus and a method for detecting liquids or substances from liquids in spatially separate reaction zones. The embodiments further relate to the use of the apparatus in a plug-in module or a chip card which can be used for rapid immunological tests, for example, with the help of a reading device. The liquids or substances from liquids are detected by means of a sensor array which includes at least two sensors and on which at least one diaphragm is arranged. Individual sensors are spatially separated from each other on a plane by means of walls. The diaphragm can be filled with liquid that is to be analyzed. Sealed reaction chambers are created when pressure is applied to the diaphragm. Pores in the diaphragm are completely closed in the zone of the walls while the pores are merely reduced in size and liquid can continue to be exchanged in zones directly above the sensors. No liquid can be exchanged between adjacent reaction chambers as long as the pressure is applied to and compresses the diaphragm. In the sealed reaction chambers, reactions can take place independently of reactions in adjacent reaction chamber. Reaction products or substances in the liquid can be measured with smaller measurement errors in sealed reaction chamber than when the liquid flows across the entire diaphragm.
摘要翻译：实施例涉及用于在空间上分离的反应区域中检测来自液体的液体或物质的装置和方法。实施例还涉及在插件模块或芯片卡中使用该装置，其可以用于例如借助于读取装置的快速免疫测试。通过包括至少两个传感器的传感器阵列检测来自液体的液体或物质，并且在该传感器阵列上布置有至少一个隔膜。单个传感器在空间上通过壁在空间上彼此分开。隔膜可以填充待分析的液体。当对隔膜施加压力时，产生密封的反应室。隔膜中的孔隙在壁的区域中完全封闭，而孔仅尺寸减小，并且液体可以在传感器正上方的区域中继续更换。只要施加压力并压缩隔膜，相邻反应室之间就不能更换液体。在密封反应室中，反应可以独立于相邻反应室中的反应进行。液体中的反应产物或物质可以在密封反应室中以比液体流过整个隔膜的更小的测量误差来测量。

26. 发明授权

US08916035B2 Arrangement and method using microsensors for measuring cell vitalities 有权
标题翻译：微传感器用于测量细胞活力的布置和方法
公开(公告)号：US08916035B2
公开(公告)日：2014-12-23
申请号：US13499376
申请日：2010-09-29
申请人： Alexander Frey , Walter Gumbrecht , Peter Paulicka , Meinrad Schienle , Daniel Sickert , Manfred Stanzel
发明人： Alexander Frey , Walter Gumbrecht , Peter Paulicka , Meinrad Schienle , Daniel Sickert , Manfred Stanzel
IPC分类号： G01N21/41 , C12M1/34 , G01N27/42 , G01N27/416 , G01N27/404
CPC分类号： G01N27/416 , C12M41/38 , C12M41/46 , G01N27/404 , G01N27/423 , G01N33/48735
摘要： An arrangement and a method measures cell vitalities with a sensor array. The sensor array is formed on a surface of a semiconductor chip. The semiconductor chip has integrated circuits and an integrated circuit is associated with each sensor of the sensor array, for processing the measurement signals of the respective sensor. The integrated circuits are formed in the semiconductor chip spatially in each case below the associated sensor and neighboring sensors of the sensor array have a center-to-center in the range of micrometers. The pH and/or pO2 can be measured in the environment of a living cell.
摘要翻译：一种安排和方法用传感器阵列测量细胞活力。传感器阵列形成在半导体芯片的表面上。半导体芯片具有集成电路，并且集成电路与传感器阵列的每个传感器相关联，用于处理相应传感器的测量信号。集成电路在空间上形成在半导体芯片中，在每个情况下在相关传感器下方，并且传感器阵列的相邻传感器具有在微米范围内的中心到中心。 pH和/或pO2可以在活细胞的环境中测量。

27. 发明申请

US20140106355A1 ARRANGEMENT AND PROCESS FOR OPTICAL ANALYSIS AND SPECIFIC ISOLATION OF BIOLOGICAL SAMPLES 有权
标题翻译：光学分析和生物样品特异性分离的安排和方法
公开(公告)号：US20140106355A1
公开(公告)日：2014-04-17
申请号：US14118723
申请日：2012-04-16
申请人： Joachim Bangert , Katja Friedrich , Walter Gumbrecht , Karsten HILtawsky , Peter Paulicka , Manfred Stanzel
发明人： Joachim Bangert , Katja Friedrich , Walter Gumbrecht , Karsten HILtawsky , Peter Paulicka , Manfred Stanzel
IPC分类号： G01N33/50
CPC分类号： G01N33/5091 , B01D63/08 , B01L3/508 , B01L2300/0681 , B01L2300/0822 , G01N1/2813 , G01N2001/045 , G01N2001/4088 , G01N2015/1006
摘要： A process is disclosed for detecting cells in a liquid sample, which includes: i) filtration of the liquid sample through a porous membrane which is suitable for retaining detectable cells, where at least one subregion of a support is configured as transparent supporting body and the membrane is arranged over its area on the transparent supporting body in such a way that detectable cells are retained on at least part of the surface of the membrane and that at least part of the sample liquid passes through the membrane, ii) application of a liquid optical medium which has essentially the same refractive index as the supporting body, and iii) optical measurement of at least a subarea of the membrane in order to detect detectable cells.
摘要翻译：公开了一种用于检测液体样品中的细胞的方法，其包括：i）通过适于保持可检测细胞的多孔膜过滤液体样品，其中支撑体的至少一个子区域被配置为透明支撑体，并且膜以透明支撑体上的区域布置，使得可检测的细胞保留在膜的至少部分表面上，并且至少部分样品液体通过膜，ii）施加液体光学介质，其具有与支撑体基本相同的折射率，以及iii）光学测量膜的至少一个子区域以便检测可检测的细胞。

28. 发明授权

US08088576B2 Method and assembly for DNA isolation with dry reagents 有权
标题翻译：用干试剂进行DNA分离的方法和装配
公开(公告)号：US08088576B2
公开(公告)日：2012-01-03
申请号：US11587581
申请日：2005-04-28
申请人： Walter Gumbrecht , Peter Paulicka , Manfred Stanzel
发明人： Walter Gumbrecht , Peter Paulicka , Manfred Stanzel
IPC分类号： C12Q1/68
CPC分类号： B01L3/502761 , B01J2219/00286 , B01J2219/00459 , B01J2219/005 , B01J2219/00648 , B01J2219/00722 , B01L7/52 , B01L2200/0668 , B01L2200/082 , B01L2200/10 , B01L2200/16 , B01L2300/0816 , B01L2400/0406 , B01L2400/0487 , C12N15/1013 , C12Q1/6806 , C12Q2563/143 , C12Q2531/113
摘要： A DNA isolation method by removal of constituents, interfering with a subsequent PCR is disclosed. According to an embodiment of the method, all substances and method steps are fully integrated into a closed unit (cartridge) for single use, which allows entry of a DNA-containing sample and DNA-binding substrates are used for isolating the released DNA. In particular, when the method is applied to DNA isolation from whole blood by disruption of white blood cells, the reagents, required for carrying out the cell disruption and other reactions, are stored in a form which is stable at room temperature. For disrupting the white blood cells and for DNA isolation, a dry stored lysis reagent is dissolved in an aqueous solution and brought into contact with the white blood cells. The corresponding assembly includes a unit, for housing DNA-containing biological containers and/or reagents, whereby at least one microchannel is provided to contain reagents, whereby the reagent is present in the microchannel as a dry mixture with a negligible vapor pressure, which forms a stable substance at room temperature.
摘要翻译：公开了通过去除组分，干扰后续PCR的DNA分离方法。根据该方法的一个实施方案，所有物质和方法步骤完全整合到用于单次使用的封闭单元（盒）中，其允许含DNA样品的进入和DNA结合底物用于分离释放的DNA。特别是，当将该方法应用于通过破坏白血细胞从全血中分离DNA时，进行细胞破碎和其它反应所需的试剂以在室温下稳定的形式储存。为了破坏白细胞和进行DNA分离，将干燥储存的裂解试剂溶解在水溶液中并与白细胞接触。相应的组件包括用于容纳含有DNA的生物容器和/或试剂的单元，由此提供至少一个微通道以容纳试剂，由此所述试剂作为干燥的混合物存在于微通道中，所述干燥混合物具有可忽略的蒸气压，其形成在室温下稳定的物质。

29. 发明申请

US20100248243A1 METHOD AND ARRANGEMENT FOR CALIBRATING A SENSOR ELEMENT 审中-公开
标题翻译：用于校准传感器元件的方法和装置
公开(公告)号：US20100248243A1
公开(公告)日：2010-09-30
申请号：US12743316
申请日：2008-10-29
申请人： Katja Friedrich , Walter Gumbrecht , Peter Paulicka , Manfred Stanzel , Renee Weber
发明人： Katja Friedrich , Walter Gumbrecht , Peter Paulicka , Manfred Stanzel , Renee Weber
IPC分类号： C12Q1/68 , C12M1/40
CPC分类号： C12Q1/6837 , C12Q2527/107
摘要： A method is disclosed for calibrating a sensor element, which has an immobilized probe oligonucleotide, via which the bonding of a target nucleic acid (Z) can be detected by the sensor. In at least one embodiment, the method includes: a) bringing the sensor element into contact with a control nucleic acid (K), the melting temperature Tm(K) of which is less than the melting temperature Tm(Z) of the target nucleic acid (Z); b) hybridizing the control nucleic acid (K) to the probe oligonucleotide at a temperature T[p] Tm(K) and detecting a negative control signal at a temperature T[n]. According to a refinement of at least one embodiment of the invention, a measuring signal of the target nucleic acid (Z) is measured at a measuring temperature T [mess], where Tm(K)
摘要翻译：公开了一种用于校准具有固定化探针寡核苷酸的传感器元件的方法，通过该探针寡核苷酸可以通过传感器检测靶核酸（Z）的结合。在至少一个实施方案中，该方法包括：a）使传感器元件与对照核酸（K）接触，其熔解温度Tm（K）小于靶核酸的解链温度Tm（Z）酸（Z）; b）在温度T [p] Tm（K）并且在温度T [n]处检测负控制信号。根据本发明的至少一个实施方案的改进，在测量温度T [mess]测量目标核酸（Z）的测量信号，其中Tm（K）

30. 发明申请

US20080193926A1 Device and method for extracting a smear sample 有权
标题翻译：用于提取涂片样品的装置和方法
公开(公告)号：US20080193926A1
公开(公告)日：2008-08-14
申请号：US11599424
申请日：2006-11-15
申请人： Klaus Abraham-Fuchs , Thomas Ehben , Walter Gumbrecht , Peter Paulicka , Sebastian Schmidt , Manfred Stanzel , Christian Zilch
发明人： Klaus Abraham-Fuchs , Thomas Ehben , Walter Gumbrecht , Peter Paulicka , Sebastian Schmidt , Manfred Stanzel , Christian Zilch
IPC分类号： C12M1/26 , C12Q1/68
CPC分类号： B01L3/502715 , A61B10/0051 , A61B10/0096 , B01L3/502738 , B01L3/502761 , B01L3/5029 , B01L2200/027 , B01L2300/042 , B01L2400/0478 , B01L2400/0633 , G01N2001/028
摘要： A device is disclosed for extracting a smear sample. The device includes a cavity, into which a sample carrier carrying a smear sample can be introduced. Liquid can be introduced into the cavity through at least one liquid feed connected to the cavity, and the liquid can be removed from the cavity through at least one liquid discharge connected to the cavity.
摘要翻译：公开了一种用于提取涂片样品的装置。该装置包括可以引入携带涂片样品的样品载体的空腔。可以通过连接到空腔的至少一个液体进料将液体引入空腔中，并且可以通过连接到空腔的至少一个液体排出物从腔中去除液体。

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