会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 22. 发明申请
    • FLUOROIMMUNOASSAY METHOD
    • 荧光分光法
    • US20120270338A1
    • 2012-10-25
    • US13510105
    • 2010-11-19
    • Hiroshi UedaRyoji AbeMasaki IharaHiroaki Takagi
    • Hiroshi UedaRyoji AbeMasaki IharaHiroaki Takagi
    • G01N21/76C07K17/02
    • G01N33/582A61K49/0058C07K16/18C07K16/44C07K2317/56C07K2317/622C07K2317/92G01N21/6428G01N33/6857G01N2021/6439
    • An object is to provide an immunoassay method requiring neither a solid-phase immobilization step nor a washing step, enabling quick and simple quantitative measurement of a target substance in a liquid phase and capable of visualizing an antigen. Such an object is attained by measuring the concentration of a target antigen present in a test substance by sequentially performing a step (a) of bringing an antibody light-chain variable region polypeptide and an antibody heavy-chain variable region polypeptide labeled with a fluorescent dye into contact with an antigen in a test substance in a liquid phase; or bringing an antibody heavy-chain variable region polypeptide and an antibody light-chain variable region polypeptide labeled with a fluorescent dye into contact with an antigen in a test substance in a liquid phase; a step (b) of measuring the fluorescence intensity of the fluorescent dye; and a step (c) of computationally obtaining the level of the antigen contained in the test substance with reference to a positive correlation between the concentration of the antigen in a liquid phase and the fluorescence intensity of the fluorescent dye.
    • 本发明的目的是提供一种不需要固相固定步骤和洗涤步骤的免疫测定方法,能够快速和简单地定量测定液相中的目标物质并能够使抗原呈现。 通过依次进行使荧光染料标记的抗体轻链可变区多肽和抗体重链可变区多肽的步骤(a),测定待测物质中存在的靶抗原的浓度, 与液相中测试物质中的抗原接触; 或使用荧光染料标记的抗体重链可变区多肽和抗体轻链可变区多肽与液相中的测试物质中的抗原接触; 测量荧光染料的荧光强度的步骤(b); 和(c)参考在液相中的抗原浓度与荧光染料的荧光强度之间的正相关性来计算获得测试物质中含有的抗原水平的步骤(c)。