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21. 发明授权

US08502963B2 Method and apparatus for analyzing individual cells or particulates using fluorescent quenching and/or bleaching 有权
公开(公告)号：US08502963B2
公开(公告)日：2013-08-06
申请号：US13645075
申请日：2012-10-04
申请人： Robert A. Levine , Stephen C. Wardlaw , Niten V. Lalpuria , Darryn W. Unfricht
发明人： Robert A. Levine , Stephen C. Wardlaw , Niten V. Lalpuria , Darryn W. Unfricht
IPC分类号： G01N33/48
CPC分类号： G01N33/49 , G01N15/1463 , G01N15/1475 , G01N21/6428 , G01N21/6456 , G01N33/48 , G01N33/5091 , G01N2015/008 , G01N2015/1477 , G01N2021/6432 , G01N2021/6439 , G01N2201/062
摘要： A method for analyzing a blood sample is provided that includes the steps of: providing a blood sample having one or more of each first and second constituents; admixing a colorant with the sample, which colorant is operative to cause the first constituents and second constituents to fluoresce and absorb light; illuminating at least a portion of the sample; e) imaging a portion of the sample; determining a fluorescence value for each the first constituents and second constituents; determining an optical density value for each of the first constituents and second constituents; and identifying the first constituents and the second constituents using the determined fluorescence and optical density values.

22. 发明申请

US20120164719A1 METHOD AND APPARATUS FOR ANALYZING INDIVIDUAL CELLS OR PARTICULATES USING FLUORESCENT QUENCHING AND/OR BLEACHING 有权
标题翻译：使用荧光猝灭和/或漂白分析个体细胞或颗粒物的方法和装置
公开(公告)号：US20120164719A1
公开(公告)日：2012-06-28
申请号：US13330236
申请日：2011-12-19
申请人： Robert A. Levine , Stephen C. Wardlaw , Niten V. Lalpuria , Darryn W. Unfricht
发明人： Robert A. Levine , Stephen C. Wardlaw , Niten V. Lalpuria , Darryn W. Unfricht
IPC分类号： C12M1/34
CPC分类号： G01N33/49 , G01N15/1463 , G01N15/1475 , G01N21/6428 , G01N21/6456 , G01N33/48 , G01N33/5091 , G01N2015/008 , G01N2015/1477 , G01N2021/6432 , G01N2021/6439 , G01N2201/062
摘要： A method for analyzing a blood sample is provided that includes the steps of: providing a blood sample having one or more of each first and second constituents; admixing a colorant with the sample, which colorant is operative to cause the first constituents and second constituents to fluoresce and absorb light; illuminating at least a portion of the sample; e) imaging a portion of the sample; determining a fluorescence value for each the first constituents and second constituents; determining an optical density value for each of the first constituents and second constituents; and identifying the first constituents and the second constituents using the determined fluorescence and optical density values.
摘要翻译：提供了一种用于分析血液样本的方法，其包括以下步骤：提供具有每个第一和第二成分中的一个或多个的血液样品; 将着色剂与样品混合，该着色剂可操作以使第一组分和第二组分发光和吸收光; 照亮样品的至少一部分; e）对样品的一部分进行成像; 确定每个第一组分和第二组分的荧光值; 确定每个第一组分和第二组分的光密度值; 以及使用所确定的荧光和光密度值来识别第一成分和第二成分。

23. 发明授权

US08081303B2 Method and apparatus for analyzing individual cells or particulates using fluorescent quenching and/or bleaching 有权
标题翻译：使用荧光猝灭和/或漂白分析单个细胞或微粒的方法和装置
公开(公告)号：US08081303B2
公开(公告)日：2011-12-20
申请号：US12408448
申请日：2009-03-20
申请人： Robert A. Levine , Stephen C. Wardlaw , Niten V. Lalpuria , Darryn W. Unfricht
发明人： Robert A. Levine , Stephen C. Wardlaw , Niten V. Lalpuria , Darryn W. Unfricht
IPC分类号： G01N33/48
CPC分类号： G01N33/49 , G01N15/1463 , G01N15/1475 , G01N21/6428 , G01N21/6456 , G01N33/48 , G01N33/5091 , G01N2015/008 , G01N2015/1477 , G01N2021/6432 , G01N2021/6439 , G01N2201/062
摘要： A method for analyzing a blood sample is provided that includes the steps of: a) providing a blood sample having one or more first constituents and one or more second constituents, which second constituents are different from the first constituents; b) depositing the sample into an analysis chamber adapted to quiescently hold the sample for analysis, the chamber defined by a first panel and a second panel, both of which panels are transparent; c) admixing a colorant with the sample, which colorant is operative to cause the first constituents and second constituents to fluoresce upon exposure to predetermined first wavelengths of light, and which colorant is operative to absorb light at one or more predetermined second wavelengths of light; d) illuminating at least a portion of the sample containing the first constituents and the second constituents at the first wavelengths and at the second wavelengths; e) imaging the at least a portion of the sample, including producing image signals indicative of fluorescent emissions from the first constituents and the second constituents and the optical density of the first constituents and the second constituents; f) determining a fluorescence value for each the first constituents and second constituents using the image signals; g) determining an optical density value for each of the first constituents and second constituents, which optical density is a function of the colorant absorbed by the constituents, using the image signals; and h) identifying the first constituents and the second constituents using the determined fluorescence and optical density values.
摘要翻译：提供了一种用于分析血液样本的方法，其包括以下步骤：a）提供具有一个或多个第一成分和一个或多个第二成分的血液样品，所述第二成分与第一成分不同; b）将样品沉积到适于静态保持样品进行分析的分析室中，所述室由第一面板和第二面板限定，两个面板都是透明的; c）将着色剂与所述样品混合，所述着色剂可操作以使得所述第一组分和第二组分在暴露于预定的第一波长的光时发荧光，并且所述着色剂可操作地吸收一个或多个预定的第二波长的光的光; d）以第一波长和第二波长照射含有第一组分和第二组分的样品的至少一部分; e）对样品的至少一部分进行成像，包括产生指示来自第一组分和第二组分的荧光发射的图像信号和第一组分和第二组分的光密度; f）使用图像信号确定每个第一成分和第二成分的荧光值; g）使用所述图像信号确定所述第一成分和第二成分中的每一个的光密度值，所述光密度值是由所述成分吸收的着色剂的函数; 和h）使用确定的荧光和光密度值来识别第一成分和第二成分。

24. 发明授权

US07951599B2 Method and apparatus for determining the hematocrit of a blood sample utilizing the intrinsic pigmentation of hemoglobin contained within the red blood cells 有权
标题翻译：利用红细胞内含有的血红蛋白的内在色素沉淀来测定血液样品的血细胞比容的方法和装置
公开(公告)号：US07951599B2
公开(公告)日：2011-05-31
申请号：US12408256
申请日：2009-03-20
申请人： Robert A. Levine , Stephen C. Wardlaw , Darryn W. Unfricht , Niten V. Lalpuria
发明人： Robert A. Levine , Stephen C. Wardlaw , Darryn W. Unfricht , Niten V. Lalpuria
IPC分类号： G01N33/48 , G01N33/86 , G01N33/72
CPC分类号： G01N33/80 , A61B5/145 , G01N15/1463 , G01N15/1475 , G01N33/48 , G01N33/49 , G01N33/721 , G01N33/86
摘要： A method for determining the hematocrit of a blood sample is provided that includes the steps of: 1) depositing the sample into an analysis chamber operable to quiescently hold the sample for analysis, the chamber defined by the interior surfaces of first and second panels and a height extending there between, wherein both panels are transparent, and the height is such that at least some of the red blood cells within the sample contact both interior surfaces of the panels and one or more lacunae within the quiescent sample extend between the interior surfaces; 2) imaging at least a portion of the quiescent sample, which sample portion contains the red blood cells and one or more lacunae to determine an optical density of the imaged portion of the sample on a per image unit basis; 3) selecting and averaging the optical density values of the image units aligned with the red blood cells contacting the interior surfaces, and assigning an upper boundary value of 100% to the average optical density value of those image units; 4) selecting the optical density values of the image units aligned with the one or more lacunae, and assigning a lower boundary value of 0% to the optical density values of those image units; and 5) determining the hematocrit of the sample by assigning relative values to the optical density value of each image of the imaged sample portion as a function of the upper and lower boundary values, and averaging the relative values.
摘要翻译：提供了一种用于确定血液样本的血细胞比容的方法，其包括以下步骤：1）将样品沉积到可操作以静态保持样品进行分析的分析室中，由第一和第二面板的内表面限定的腔室和高度在其间延伸，其中两个面板是透明的，并且高度使得样品中的至少一些红细胞接触面板的两个内表面，并且静止样品内的一个或多个空隙在内表面之间延伸; 2）对静止样品的至少一部分进行成像，该样品部分包含红细胞和一个或多个空隙，以基于每个图像单位确定样品的成像部分的光密度; 3）选择和平均与内表面接触的红细胞对准的图像单元的光密度值，并将上限边界值分配给这些图像单元的平均光密度值; 4）选择与一个或多个空白对齐的图像单元的光密度值，并为这些图像单元的光密度值分配0％的下边界值; 以及5）通过将所述成像样本部分的每个图像的光密度值分配相对值作为上边界值和下边界值的函数来确定样本的血细胞比容，并平均相对值。

25. 发明申请

US20150097939A9 METHOD AND APPARATUS FOR DETERMINING A FOCAL POSITION OF AN IMAGING DEVICE ADAPTED TO IMAGE A BIOLOGIC SAMPLE 审中-公开
标题翻译：用于确定适用于图像生物样品的成像装置的焦点位置的方法和装置
公开(公告)号：US20150097939A9
公开(公告)日：2015-04-09
申请号：US13919666
申请日：2013-06-17
申请人： Stephen C. Wardlaw , Niten V. Lalpuria , Darryn W. Unfricht
发明人： Stephen C. Wardlaw , Niten V. Lalpuria , Darryn W. Unfricht
IPC分类号： G02B21/36
CPC分类号： G02B21/365 , G02B21/244
摘要： A method and apparatus for focusing a device for imaging a biologic sample is provided. A method aspect includes the steps of: disposing lenslets within a biologic sample, which lenslets have a height and a refractive index, which refractive index is different from that of the sample, wherein one or both of the imaging device and the sample are relatively locatable so a focal position of the imaging device can be moved along the height of the lenslets; imaging a portion of the sample including lenslets using transmittance at one or more wavelengths; determining an average light transmittance intensity of the sample at the wavelengths; determining an average light transmittance intensity of a region of each lenslet at the wavelengths; and determining the focal position of the imaging device using the average light transmittance intensity of the sample and the average light transmittance intensity of the region of the lenslets.
摘要翻译：提供了用于聚焦用于对生物样品进行成像的装置的方法和装置。方法方面包括以下步骤：在生物样品中设置小透镜，哪些小透镜具有高度和折射率，其折射率与样品的折射率不同，其中成像装置和样品中的一个或两个可相对定位因此成像装置的焦点位置可以沿着小透镜的高度移动; 使用一个或多个波长的透射率成像包括小透镜的样品的一部分; 确定所述样品在所述波长处的平均光透射强度; 确定每个小透镜在该波长处的区域的平均光透射强度; 以及使用样品的平均光透射强度和小透镜区域的平均透光强度来确定成像装置的焦点位置。

26. 发明申请

US20140368631A1 METHOD AND APPARATUS FOR DETERMINING A FOCAL POSITION OF AN IMAGING DEVICE ADAPTED TO IMAGE A BIOLOGIC SAMPLE 审中-公开
标题翻译：用于确定适用于图像生物样品的成像装置的焦点位置的方法和装置
公开(公告)号：US20140368631A1
公开(公告)日：2014-12-18
申请号：US13919666
申请日：2013-06-17
申请人： Stephen C. Wardlaw , Niten V. Lalpuria , Darryn W. Unfricht
发明人： Stephen C. Wardlaw , Niten V. Lalpuria , Darryn W. Unfricht
IPC分类号： G02B21/36
CPC分类号： G02B21/365 , G02B21/244
摘要： A method and apparatus for focusing a device for imaging a biologic sample is provided. A method aspect includes the steps of: disposing lenslets within a biologic sample, which lenslets have a height and a refractive index, which refractive index is different from that of the sample, wherein one or both of the imaging device and the sample are relatively locatable so a focal position of the imaging device can be moved along the height of the lenslets; imaging a portion of the sample including lenslets using transmittance at one or more wavelengths; determining an average light transmittance intensity of the sample at the wavelengths; determining an average light transmittance intensity of a region of each lenslet at the wavelengths; and determining the focal position of the imaging device using the average light transmittance intensity of the sample and the average light transmittance intensity of the region of the lenslets.
摘要翻译：提供了用于聚焦用于对生物样品进行成像的装置的方法和装置。方法方面包括以下步骤：在生物样品中设置小透镜，哪些小透镜具有高度和折射率，其折射率与样品的折射率不同，其中成像装置和样品中的一个或两个可相对定位因此成像装置的焦点位置可以沿着小透镜的高度移动; 使用一个或多个波长的透射率成像包括小透镜的样品的一部分; 确定所述样品在所述波长处的平均光透射强度; 确定每个小透镜在该波长处的区域的平均光透射强度; 以及使用样品的平均光透射强度和小透镜区域的平均透光强度来确定成像装置的焦点位置。

27. 发明授权

US08045165B2 Method and apparatus for determining a focal position of an imaging device adapted to image a biologic sample 有权
标题翻译：用于确定适于对生物样本进行成像的成像装置的焦点位置的方法和装置
公开(公告)号：US08045165B2
公开(公告)日：2011-10-25
申请号：US12408479
申请日：2009-03-20
申请人： Stephen C. Wardlaw , Niten V. Lalpuria , Darryn W. Unfricht
发明人： Stephen C. Wardlaw , Niten V. Lalpuria , Darryn W. Unfricht
IPC分类号： G01N21/00
CPC分类号： G02B21/241
摘要： A method and apparatus for focusing a device for imaging a biologic sample is provided. A method aspect of the disclosure includes the steps of: 1) disposing lenslets within a field of a biologic sample, which lenslets have a height, and have a refractive index and which refractive index is different from that of the sample, wherein one or both of the imaging device and the sample are relatively locatable so a focal position of the imaging device can be moved along the height of the lenslets; 2) imaging at least a portion of the sample including a plurality of lenslets using transmittance at one or more predetermined wavelengths; 3) determining an average light transmittance intensity of the sample at the wavelengths; 4) determining an average light transmittance intensity of a region of each lenslet at the wavelengths; and 5) determining the focal position of the imaging device using the average light transmittance intensity of the sample and the average light transmittance intensity of the region of the lenslets.
摘要翻译：提供了用于聚焦用于对生物样品进行成像的装置的方法和装置。本公开的方法方面包括以下步骤：1）在生物样品的场内设置小透镜，哪些小透镜具有高度，并且具有折射率并且折射率不同于样品的折射率，其中一个或两个成像装置和样品相对定位，使得成像装置的焦点位置可以沿着小透镜的高度移动; 2）使用一个或多个预定波长的透射率对包含多个小透镜的样品的至少一部分进行成像; 3）确定样品在波长处的平均光透射强度; 4）确定每个小透镜在该波长处的区域的平均透光强度; 以及5）使用样品的平均光透射强度和小透镜区域的平均光透射强度来确定成像装置的焦点位置。

28. 发明申请

US20090237665A1 METHOD AND APPARATUS FOR DETERMINING A FOCAL POSITION OF AN IMAGING DEVICE ADAPTED TO IMAGE A BIOLOGIC SAMPLE 有权
标题翻译：用于确定适用于图像生物样品的成像装置的焦点位置的方法和装置
公开(公告)号：US20090237665A1
公开(公告)日：2009-09-24
申请号：US12408479
申请日：2009-03-20
申请人： Stephen C. Wardlaw , Niten V. Lalpuria , Darryn W. Unfricht
发明人： Stephen C. Wardlaw , Niten V. Lalpuria , Darryn W. Unfricht
IPC分类号： G01N21/59
CPC分类号： G02B21/241
摘要： A method and apparatus for focusing a device for imaging a biologic sample is provided. A method aspect of the disclosure includes the steps of: 1) disposing lenslets within a field of a biologic sample, which lenslets have a height, and have a refractive index and which refractive index is different from that of the sample, wherein one or both of the imaging device and the sample are relatively locatable so a focal position of the imaging device can be moved along the height of the lenslets; 2) imaging at least a portion of the sample including a plurality of lenslets using transmittance at one or more predetermined wavelengths; 3) determining an average light transmittance intensity of the sample at the wavelengths; 4) determining an average light transmittance intensity of a region of each lenslet at the wavelengths; and 5) determining the focal position of the imaging device using the average light transmittance intensity of the sample and the average light transmittance intensity of the region of the lenslets.
摘要翻译：提供了用于聚焦用于对生物样品进行成像的装置的方法和装置。本公开的方法方面包括以下步骤：1）在生物样品的场内设置小透镜，哪些小透镜具有高度，并且具有折射率并且折射率不同于样品的折射率，其中一个或两个成像装置和样品相对定位，使得成像装置的焦点位置可以沿着小透镜的高度移动; 2）使用一个或多个预定波长的透射率对包含多个小透镜的样品的至少一部分进行成像; 3）确定样品在波长处的平均光透射强度; 4）确定每个小透镜在该波长处的区域的平均透光强度; 以及5）使用样品的平均光透射强度和小透镜区域的平均光透射强度来确定成像装置的焦点位置。

29. 发明授权

US08467063B2 Method and apparatus for determining a focal position of an imaging device adapted to image a biologic sample 有权
标题翻译：用于确定适于对生物样本进行成像的成像装置的焦点位置的方法和装置
公开(公告)号：US08467063B2
公开(公告)日：2013-06-18
申请号：US13279991
申请日：2011-10-24
申请人： Stephen C. Wardlaw , Niten V. Lalpuria , Darryn W. Unfricht
发明人： Stephen C. Wardlaw , Niten V. Lalpuria , Darryn W. Unfricht
IPC分类号： G01N21/00
CPC分类号： G02B21/241
摘要： A method and apparatus for focusing a device for imaging a biologic sample is provided. A method aspect includes the steps of: disposing lenslets within a biologic sample, which lenslets have a height and a refractive index, which refractive index is different from that of the sample, wherein one or both of the imaging device and the sample are relatively locatable so a focal position of the imaging device can be moved along the height of the lenslets; imaging a portion of the sample including lenslets using transmittance at one or more wavelengths; determining an average light transmittance intensity of the sample at the wavelengths; determining an average light transmittance intensity of a region of each lenslet at the wavelengths; and determining the focal position of the imaging device using the average light transmittance intensity of the sample and the average light transmittance intensity of the region of the lenslets.
摘要翻译：提供了用于聚焦用于对生物样品进行成像的装置的方法和装置。方法方面包括以下步骤：在生物样品中设置小透镜，哪些小透镜具有高度和折射率，其折射率与样品的折射率不同，其中成像装置和样品中的一个或两个可相对定位因此成像装置的焦点位置可以沿着小透镜的高度移动; 使用一个或多个波长的透射率成像包括小透镜的样品的一部分; 确定所述样品在所述波长处的平均光透射强度; 确定每个小透镜在该波长处的区域的平均光透射强度; 以及使用样品的平均光透射强度和小透镜区域的平均透光强度来确定成像装置的焦点位置。

30. 发明申请

US20120099108A1 METHOD AND APPARATUS FOR DETERMINING A FOCAL POSITION OF AN IMAGING DEVICE ADAPTED TO IMAGE A BIOLOGIC SAMPLE 有权
标题翻译：用于确定适用于图像生物样品的成像装置的焦点位置的方法和装置
公开(公告)号：US20120099108A1
公开(公告)日：2012-04-26
申请号：US13279991
申请日：2011-10-24
申请人： Stephen C. Wardlaw , Niten V. Lalpuria , Darryn W. Unfricht
发明人： Stephen C. Wardlaw , Niten V. Lalpuria , Darryn W. Unfricht
IPC分类号： G01N21/59 , G01N21/01
CPC分类号： G02B21/241
摘要： A method and apparatus for focusing a device for imaging a biologic sample is provided. A method aspect includes the steps of: disposing lenslets within a biologic sample, which lenslets have a height and a refractive index, which refractive index is different from that of the sample, wherein one or both of the imaging device and the sample are relatively locatable so a focal position of the imaging device can be moved along the height of the lenslets; imaging a portion of the sample including lenslets using transmittance at one or more wavelengths; determining an average light transmittance intensity of the sample at the wavelengths; determining an average light transmittance intensity of a region of each lenslet at the wavelengths; and determining the focal position of the imaging device using the average light transmittance intensity of the sample and the average light transmittance intensity of the region of the lenslets.
摘要翻译：提供了用于聚焦用于对生物样品进行成像的装置的方法和装置。方法方面包括以下步骤：在生物样品中设置小透镜，哪些小透镜具有高度和折射率，该折射率与样品的折射率不同，其中成像装置和样品中的一个或两个可相对定位因此成像装置的焦点位置可以沿着小透镜的高度移动; 使用一个或多个波长的透射率成像包含小透镜的样品的一部分; 确定所述样品在所述波长处的平均光透射强度; 确定每个小透镜在该波长处的区域的平均光透射强度; 以及使用样品的平均光透射强度和小透镜区域的平均透光强度来确定成像装置的焦点位置。

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