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21. 发明授权

US07312062B2 Lipolytic enzyme variants 有权
标题翻译：脂解酶变体
公开(公告)号：US07312062B2
公开(公告)日：2007-12-25
申请号：US10779427
申请日：2004-11-22
申请人： Kirsten Bojsen , Allan Svendsen , Claus Crone Fuglsang , Shamkant Anant Patkar , Kim Borch , Jesper Vind , Andreas Petri , Sanne O. Schrøder Glad , Gitte Budolfsen
发明人： Kirsten Bojsen , Allan Svendsen , Claus Crone Fuglsang , Shamkant Anant Patkar , Kim Borch , Jesper Vind , Andreas Petri , Sanne O. Schrøder Glad , Gitte Budolfsen
IPC分类号： C12N9/16 , C12N9/18 , D06M16/00 , A21D10/00
CPC分类号： C12Y301/01003 , A21D8/042 , C11B3/003 , C11D3/38627 , C12N9/20 , C12P9/00
摘要： The substrate specificity of a lipolytic enzyme can be modified by making alterations to the amino acid sequence in a defined region of the lipolytic enzyme, so as to increase the level of a desired activity or to decrease the level of an undesired activity. Thus, the inventors have developed lipolytic enzyme variants with a modified amino acid sequence with a substrate specificity which can be tailored for specific uses.
摘要翻译：可以通过改变脂肪分解酶的限定区域中的氨基酸序列来修饰脂解酶的底物特异性，从而增加所需活性的水平或降低不需要的活性的水平。因此，本发明人已经开发了具有修饰的氨基酸序列的脂肪分解酶变体，其具有底物特异性，其可以针对特定用途进行定制。

22. 发明授权

US06967035B2 Method of improving dough and bread quality 失效
公开(公告)号：US06967035B2
公开(公告)日：2005-11-22
申请号：US10150429
申请日：2002-05-17
申请人： Kirsten Bojsen , Charlotte Horsmans Poulsen , Jorn Borch Soe
发明人： Kirsten Bojsen , Charlotte Horsmans Poulsen , Jorn Borch Soe
IPC分类号： A21D2/16 , A21D2/26 , A21D8/04 , A23L7/104 , A23L7/109
CPC分类号： A21D2/16 , A21D2/26 , A21D8/042 , A23L7/107 , A23L7/109 , C12Y101/03005 , C12Y301/01003 , C12Y301/01004 , C12Y301/01026
摘要： A method of preparing a flour dough, said method comprising adding to the dough components an enzyme that under dough conditions is capable of hydrolysing a glycolipid and a phospholipid, wherein said enzyme is incapable, or substantially incapable, of hydrolysing a triglyceride and/or a 1-monoglyceride, or a composition comprising said enzyme, and mixing the dough components to obtain the dough.

23. 发明授权

US06624129B1 Lipase variant 有权
标题翻译：脂肪酶变体
公开(公告)号：US06624129B1
公开(公告)日：2003-09-23
申请号：US09630250
申请日：2000-08-01
申请人： Kim Borch , Jesper Vind , Allan Svendsen , Dorte Aaby Halkier , Shamkant Anant Patkar , Kirsten Bojsen
发明人： Kim Borch , Jesper Vind , Allan Svendsen , Dorte Aaby Halkier , Shamkant Anant Patkar , Kirsten Bojsen
IPC分类号： C12N916
CPC分类号： C11D1/83 , C11D1/02 , C11D3/38627 , C12N9/20
摘要： The present invention relates to lipase variants having at least 90% identity to the wild-type lipase derived from Humicola lanuginosa strain DSM 4109 and having a certain distribution of electrically charged amino acids. The present invention also relates to detergents comprising such lipases.
摘要翻译：本发明涉及与源自Humicola lanuginosa菌株DSM 4109的野生型脂肪酶具有至少90％同一性且具有一定分布的带电氨基酸的脂肪酶变体。本发明还涉及包含这种脂肪酶的洗涤剂。

24. 发明授权

US08679774B2 Lipolytic enzyme variants 有权
标题翻译：脂解酶变体
公开(公告)号：US08679774B2
公开(公告)日：2014-03-25
申请号：US12849911
申请日：2010-08-04
申请人： Kirsten Bojsen , Allan Svendsen , Claus Crone Fuglsang , Shamkant Anant Patkar , Kim Borch , Jesper Vind , Andreas Petri , Sanne O. Schroder Glad , Gitte Budolfsen
发明人： Kirsten Bojsen , Allan Svendsen , Claus Crone Fuglsang , Shamkant Anant Patkar , Kim Borch , Jesper Vind , Andreas Petri , Sanne O. Schroder Glad , Gitte Budolfsen
IPC分类号： C12Q1/34 , C12N9/20 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12Y301/01003 , A21D8/042 , C11B3/003 , C11D3/38627 , C12N9/20 , C12P9/00
摘要： The substrate specificity of a lipolytic enzyme can be modified by making alterations to the amino acid sequence in a defined region of the lipolytic enzyme, so as to increase the level of a desired activity or to decrease the level of an undesired activity. Thus, the inventors have developed lipolytic enzyme variants with a modified amino acid sequence with a substrate specificity which can be tailored for specific uses.
摘要翻译：可以通过改变脂肪分解酶的限定区域中的氨基酸序列来修饰脂解酶的底物特异性，从而增加所需活性的水平或降低不需要的活性的水平。因此，本发明人已经开发了具有修饰的氨基酸序列的脂肪分解酶变体，其具有底物特异性，其可以针对特定用途进行定制。

25. 发明授权

US08143048B2 Exo-specific amylase polypeptides, nucleic acids encoding those polypeptides and uses thereof 有权
标题翻译：特异性淀粉酶多肽，编码那些多肽的核酸及其用途
公开(公告)号：US08143048B2
公开(公告)日：2012-03-27
申请号：US10886903
申请日：2004-07-07
申请人： Karsten Matthias Kragh , Kirsten Bojsen , Casper Tune Berg , Patrick M. F. Derkx , Anja H. Kellett-Smith , Charlotte Refdahl Thoudahl , Carol Fioresi , Wei Liu , Andrew Shaw , Gijsbert Gerritse
发明人： Karsten Matthias Kragh , Kirsten Bojsen , Casper Tune Berg , Patrick M. F. Derkx , Anja H. Kellett-Smith , Charlotte Refdahl Thoudahl , Carol Fioresi , Wei Liu , Andrew Shaw , Gijsbert Gerritse
IPC分类号： C12N9/28 , C12N15/00 , C12N9/88
CPC分类号： C12N9/2417
摘要： This invention relates to amylase polypeptides, and nucleic acids encoding the polpypeptides and uses thereof. The amylases of the present invention have been engineered to have more beneficial qualities. Specifically, the amylases of the current invention show an altered exospecifity.
摘要翻译：本发明涉及淀粉酶多肽和编码多肽的核酸及其用途。本发明的淀粉酶已被设计成具有更多的有益品质。具体地说，本发明的淀粉酶表现出改变的促进作用。

26. 发明授权

US08129511B2 Modified amylases, nucleic acids encoding those amylases and uses thereof 有权
标题翻译：改性淀粉酶，编码这些淀粉酶的核酸及其用途
公开(公告)号：US08129511B2
公开(公告)日：2012-03-06
申请号：US13023061
申请日：2011-02-08
申请人： Karsten Matthias Kragh , Bo Spange Sørensen , Casper Tune Berg , Kirsten Bojsen , Patrick M. F. Derkx , Carol Fioresi , Anja H. Kellett-Smith , Charlotte Refdahl Thoudahl , Oene R. Veltman , Gijsbert Gerritse , Wei Liu , Andrew Shaw
发明人： Karsten Matthias Kragh , Bo Spange Sørensen , Casper Tune Berg , Kirsten Bojsen , Patrick M. F. Derkx , Carol Fioresi , Anja H. Kellett-Smith , Charlotte Refdahl Thoudahl , Oene R. Veltman , Gijsbert Gerritse , Wei Liu , Andrew Shaw
IPC分类号： C07H21/00 , C12N1/21 , C12N1/15 , C12N1/19
CPC分类号： C12N9/2417 , A21D8/042 , A23L29/06 , A23L29/35 , A23L33/17 , A23V2002/00 , A23V2300/21
摘要： This invention relates to nucleic acids encoding amylase polypeptides, the encoded polypeptides and uses thereof. The amylases of the present invention have been engineered to have more beneficial qualities. Specifically, the amylases of the current invention show an altered exo-specificity and/or thermostability. Some embodiments of the invention relate to said polypeptides and nucleic acids and their uses as non-maltogenic exoamylases in producing food products.
摘要翻译：本发明涉及编码淀粉酶多肽的核酸，编码的多肽及其用途。本发明的淀粉酶已被设计成具有更多的有益品质。具体地，本发明的淀粉酶显示出改变的外部特异性和/或热稳定性。本发明的一些实施方案涉及所述多肽和核酸及其在制备食品中的非麦芽糖胞外淀粉酶的用途。

27. 发明授权

US07632669B2 Lipolytic enzyme variants 有权
标题翻译：脂解酶变体
公开(公告)号：US07632669B2
公开(公告)日：2009-12-15
申请号：US11951597
申请日：2007-12-06
申请人： Kirsten Bojsen , Allan Svendsen , Claus Crone Fuglsang , Shamkant Anant Patkar , Kim Borch , Jesper Vind , Andreas Petri , Sanne O. Schroder Glad , Gitte Budolfsen
发明人： Kirsten Bojsen , Allan Svendsen , Claus Crone Fuglsang , Shamkant Anant Patkar , Kim Borch , Jesper Vind , Andreas Petri , Sanne O. Schroder Glad , Gitte Budolfsen
IPC分类号： C12N9/20
CPC分类号： C12Y301/01003 , A21D8/042 , C11B3/003 , C11D3/38627 , C12N9/20 , C12P9/00
摘要： The substrate specificity of a lipolytic enzyme can be modified by making alterations to the amino acid sequence in a defined region of the lipolytic enzyme, so as to increase the level of a desired activity or to decrease the level of an undesired activity. Thus, the inventors have developed lipolytic enzyme variants with a modified amino acid sequence with a substrate specificity which can be tailored for specific uses.
摘要翻译：可以通过改变脂肪分解酶的限定区域中的氨基酸序列来修饰脂解酶的底物特异性，从而增加所需活性的水平或降低不需要的活性的水平。因此，本发明人已经开发了具有修饰的氨基酸序列的脂肪分解酶变体，其具有底物特异性，其可以针对特定用途进行定制。

28. 发明申请

US20090047384A1 LIPOLYTIC ENZYME VARIANTS 有权
公开(公告)号：US20090047384A1
公开(公告)日：2009-02-19
申请号：US12245082
申请日：2008-10-03
申请人： Kirsten Bojsen , Allan Svendsen , Claus Crone Fuglsang , Shamkant Anant Patkar , Kim Borch , Jesper Vind , Andreas Petri , Sanne O. Schroder Glad , Gitte Budolfsen
发明人： Kirsten Bojsen , Allan Svendsen , Claus Crone Fuglsang , Shamkant Anant Patkar , Kim Borch , Jesper Vind , Andreas Petri , Sanne O. Schroder Glad , Gitte Budolfsen
IPC分类号： A21D8/04
CPC分类号： C12Y301/01003 , A21D8/042 , C11B3/003 , C11D3/38627 , C12N9/20 , C12P9/00
摘要： The substrate specificity of a lipolytic enzyme can be modified by making alterations to the amino acid sequence in a defined region of the lipolytic enzyme, so as to increase the level of a desired activity or to decrease the level of an undesired activity. Thus, the inventors have developed lipolytic enzyme variants with a modified amino acid sequence with a substrate specificity which can be tailored for specific uses.
摘要翻译：可以通过改变脂肪分解酶的限定区域中的氨基酸序列来修饰脂解酶的底物特异性，从而增加所需活性的水平或降低不需要的活性的水平。因此，本发明人已经开发了具有修饰的氨基酸序列的脂肪分解酶变体，其具有底物特异性，其可以针对特定用途进行定制。

29. 发明申请

US20080131951A1 Lipolytic Enzyme Variants 有权
标题翻译：脂解酶变体
公开(公告)号：US20080131951A1
公开(公告)日：2008-06-05
申请号：US11951597
申请日：2007-12-06
申请人： Kirsten Bojsen , Allan Svendsen , Claus Crone Fuglsang , Shamkant Anant Patkar , Kim Borch , Jesper Vind , Andreas Petri , Sanne O. Schroder Glad , Gitte Budolfsen
发明人： Kirsten Bojsen , Allan Svendsen , Claus Crone Fuglsang , Shamkant Anant Patkar , Kim Borch , Jesper Vind , Andreas Petri , Sanne O. Schroder Glad , Gitte Budolfsen
IPC分类号： C12N9/16
CPC分类号： C12Y301/01003 , A21D8/042 , C11B3/003 , C11D3/38627 , C12N9/20 , C12P9/00
摘要： The substrate specificity of a lipolytic enzyme can be modified by making alterations to the amino acid sequence in a defined region of the lipolytic enzyme, so as to increase the level of a desired activity or to decrease the level of an undesired activity. Thus, the inventors have developed lipolytic enzyme variants with a modified amino acid sequence with a substrate specificity which can be tailored for specific uses.
摘要翻译：可以通过改变脂肪分解酶的限定区域中的氨基酸序列来修饰脂解酶的底物特异性，从而增加所需活性的水平或降低不需要的活性的水平。因此，本发明人已经开发了具有修饰的氨基酸序列的脂肪分解酶变体，其具有底物特异性，其可以针对特定用途进行定制。

30. 发明授权

US06444878B1 Method of plant selection using glucosamine-6-phosphate deaminase 失效
标题翻译：使用葡糖胺-6-磷酸脱氨酶的植物选择方法
公开(公告)号：US06444878B1
公开(公告)日：2002-09-03
申请号：US09367293
申请日：1999-12-23
申请人： Iain A. Donaldson , Kirsten Bojsen , Kirsten Jorgensen , Morten Jorsboe
发明人： Iain A. Donaldson , Kirsten Bojsen , Kirsten Jorgensen , Morten Jorsboe
IPC分类号： C12N1582
CPC分类号： C12N15/8241 , C12N9/90 , C12N15/8209
摘要： A selection method for selecting from a population of plant cells one or more genetically transformed plant cells is described. In the method, the population of plant cells includes selectable genetically transformed plant cells and possible non-transformed plant cells. Each of the selectable genetically transformed plant cells comprises a first expressible nucleotide sequence and optionally a second expressible nucleotide sequence. In the method, a component or a metabolic derivative thereof when present in a low concentration in a medium is a nutrient for both the selectable genetically transformed plant cells and the non-transformed plant cells. In the method, the component or the metabolic derivative thereof when present in a high concentration in a medium is toxic to the non-transformed plant cells. The first nucleotide sequence codes for a gene product having glucosamine-6-phosphate deaminase activity which is capable of converting the component or the metabolic derivative thereof when present in a high concentration in a medium to a nutrient for the selectable genetically transformed plant cells. The method includes the step of introducing the population of plant cells to a medium, wherein the medium includes a high concentration of the component or the metabolic derivative thereof. In the method, the component or the metabolic derivative thereof is a source of both carbohydrate and nitrogen for the selectable genetically transformed plant cells.
摘要翻译：描述了从植物细胞群体中选择一个或多个遗传转化的植物细胞的选择方法。在该方法中，植物细胞群体包括选择性遗传转化的植物细胞和可能的未转化植物细胞。每个可选择的经遗传转化的植物细胞包含第一可表达核苷酸序列和任选的第二可表达核苷酸序列。在该方法中，当在培养基中以低浓度存在时，其组分或代谢衍生物是可选择的遗传转化的植物细胞和未转化的植物细胞的营养物。在该方法中，当在培养基中以高浓度存在时，组分或其代谢衍生物对未转化的植物细胞是有毒的。第一核苷酸序列编码具有葡糖胺-6-磷酸脱氨酶活性的基因产物，其能够在培养基中以高浓度存在时将组分或其代谢衍生物转化为可选择的遗传转化植物细胞的营养物。该方法包括将植物细胞群引入培养基的步骤，其中培养基包含高浓度的组分或其代谢衍生物。在该方法中，组分或其代谢衍生物是用于可选遗传转化的植物细胞的碳水化合物和氮源。

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