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    • 21. 发明申请
    • METHOD OF AND KIT FOR TESTING IN VITRO STIMULATION
    • 用于体外刺激测试的方法和试剂盒
    • WO1993004369A1
    • 1993-03-04
    • PCT/JP1992001045
    • 1992-08-19
    • SHISEIDO COMPANY, LTD.HAYASHI, ToshikatsuITAGAKI, HiroshiTAMURA, UheiKATO, Shinobu
    • SHISEIDO COMPANY, LTD.
    • G01N33/15
    • G01N33/5014
    • This invention provides a method of in vitro testing for evaluating a degree of stimulation imposed on an organism by an alien substance. The invention provides an in vitro stimulation testing method comprising such steps that, in aqueous solution, an alien substance (substance to be tested) is brought into contact with protein having absorption spectrum at its visible part, variation in absorption spectrum resulting from said contact is measured, and then an amount of said variation is evaluated as an index of stimulation on the organism caused by the substance to be tested. Further provided by this invention is a method of testing an organism stimulation by an alien substance which employs a combination of the abovesaid method with other in vitro stimulation testing methods. A kit for performing such testing methods is also provided. The methods of this invention show a satisfactory correlation with the Draze method as an in vivo testing method.
    • 本发明提供一种体外测试方法,用于评估外来物质对生物体施加的刺激程度。 本发明提供了一种体外刺激试验方法,其包括以下步骤:在水溶液中,外来物质(待测试物质)与其可见部分具有吸收光谱的蛋白质接触,所述接触引起的吸收光谱的变化为 测量,然后评估所述变化的量作为由待测物质引起的对生物体的刺激指数。 本发明进一步提供了一种通过使用上述方法与其它体外刺激测试方法组合的外来物质来测试生物体刺激的方法。 还提供了用于执行这种测试方法的套件。 本发明的方法表现出与Draze方法作为体内测试方法的令人满意的相关性。