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21. 发明专利

ZA201407412B METHOD AND COMPOSITIONS FOR PREVENTING STEM CELL DISRUPTION AND AGGREGATION 未知
公开(公告)号：ZA201407412B
公开(公告)日：2016-09-28
申请号：ZA201407412
申请日：2014-10-13
申请人： RA JEONG CHAN
发明人： SHIN II SEOB , RA JEONG CHAN , KANG SUNG KEUN
IPC分类号： A61K35/28
摘要： There is provided a method for preventing disruption and aggregation of stem cells. In addition, there is provided a stem cell composition for intravascular administration, in which stem cells are suspended in a solution containing aspirin. Further, there is provided a composition for preventing disruption or aggregation of stem cells, in which stem cells are suspended in a solution containing aspirin. According to the present invention, disruption and aggregation of cells may be prevented during transport or storage, such that the administered stem cells may stably reach the target tissue and exhibit the activity thereof in a more efficient manner, thereby remarkably increasing the effect of cell therapy using stem cells.

22. 发明公开

KR20100096447A Cosmetic composition comprising matrials cultured adult stem cells derived from swine placenta tissue and proteins extracted therefrom 审中-公开
标题翻译：化学成分包含从SWINE PLACENTA组织和蛋白质提取的基质培养的成熟干细胞
公开(公告)号：KR20100096447A
公开(公告)日：2010-09-02
申请号：KR20090015330
申请日：2009-02-24
申请人： RNL BIO CO LTD
发明人： RA JEONG CHAN , KANG SUNG KEUN , WOO SANG KYU , YIM CHA OK
IPC分类号： A61K8/98 , A61Q19/08 , C12N5/02 , C12N5/07
CPC分类号： A61K8/98
摘要： PURPOSE: A cosmetic composition containing a porcine placenta-derived adult stem cell culture liquid or a protein thereof is provided to enhance the anti-wrinkle and anti-aging properties of cosmetics. CONSTITUTION: A functional cosmetic composition contains a porcine placenta-derived adult stem cell culture liquid as an active ingredient. The functional cosmetic composition additionally contains a protein extracted from the adult stem cells. peptides, or amino acids of the protein. The cosmetic composition is used in the form of a lotion, emulsion, cream, gel, essence, or pack. A method for manufacturing the culture liquid comprises: a step of isolating amnion from porcine placenta; a step of collecting adult stem cells from the amnion; and a step of culturing the adult stem cells in a medium containing bFGF.
摘要翻译：目的：提供含有猪胎盘来源的成体干细胞培养液或其蛋白质的化妆品组合物，以提高化妆品的抗皱和抗老化性能。构成：功能性化妆品组合物含有猪胎盘来源的成体干细胞培养液作为活性成分。功能性化妆品组合物另外含有从成体干细胞提取的蛋白质。肽或蛋白质的氨基酸。化妆品组合物以洗剂，乳液，乳膏，凝胶，精华液或包装的形式使用。培养液的制造方法包括：从猪胎盘中分离羊膜的步骤; 从羊膜采集成体干细胞的一个步骤; 以及在含有bFGF的培养基中培养成体干细胞的步骤。

23. 发明公开

KR20100081805A Method for producing cloned canidae using nuclear transfer of somatic cells or stem cells 审中-公开
标题翻译：使用原代细胞或干细胞的核转移生产克隆的癌症的方法
公开(公告)号：KR20100081805A
公开(公告)日：2010-07-15
申请号：KR20090001211
申请日：2009-01-07
申请人： SNU R&DB FOUNDATION , RNL BIO CO LTD
发明人： LEE BYEONG CHUN , JANG GOO , KOO OK JAE , OH HYUN JU , HONG SO GUN , PARK JUNG EUN , KIM MIN JUNG , RA JEONG CHAN
IPC分类号： C12N15/09 , A01K67/027 , C12N5/10
摘要： PURPOSE: A method for producing cloned dog using stem cells or somatic cells of canidae animal is provided to develop xenogeneic transplantation and disease model animal. CONSTITUTION: A method for preparing nuclear transplantation egg of canidae comprises a step of transplanting nuclear donor cell to enucleated oocytes of meiosis telophase. The nuclear donor cell is mitosis G2/M phase somatic cells or stem cells. The stem cells are derived embryo or adult. The adult stem cells are bone marrow, umbilical cord blood, blood, fat, skin, placenta, or uterin. The somatic cells of mitosis G2/M phase are obtained at a process of adding cell cycle synchronization inducing agent and culturing. The cell cycle synchronization inducing agent is roscovitine, cyclohexamide, DMSO, butyrolactone I, aphidicolin, demecolcine, mimosine, colchicines, or Hoechst 33342.
摘要翻译：目的：提供使用犬科动物的干细胞或体细胞产生克隆狗的方法，以开发异种移植和疾病模型动物。构成：一种用于制备犬蛔虫核移植卵的方法，包括将核供体细胞移植到减数分裂端粒酶的去核卵母细胞的步骤。核供体细胞是有丝分裂G2 / M期体细胞或干细胞。干细胞来源于胚胎或成体。成体干细胞是骨髓，脐带血，血液，脂肪，皮肤，胎盘或子宫。在添加细胞周期同步诱导剂和培养的过程中获得有丝分裂G2 / M期的体细胞。细胞周期同步诱导剂是roscovitine，环己酰胺，DMSO，丁内酯I，aphidicolin，demecolcine，mimosine，秋水仙碱或Hoechst 33342。

24. 发明专利

AU2013250089B2 Method for manufacturing stem cell having appropriate size for intravascular administration 未知
公开(公告)号：AU2013250089B2
公开(公告)日：2016-08-18
申请号：AU2013250089
申请日：2013-04-17
申请人： R BIO CO LTD , RA JEONG CHAN
发明人： RA JEONG CHAN , KANG SUNG KEUN , SHIN IL SEOB
IPC分类号： C12N5/074 , C12N5/02 , C12N5/0775
摘要： The present invention relates to a method for manufacturing a stem cell having an appropriate size for intravascular administration, and preferably to a method for manufacturing a stem cell with a diameter ranging from 11‑16 µm. Additionally, the present invention relates to media for manufacturing a stem cell having an appropriate size for intravascular administration. According to the present invention, a stem cell having an appropriate size for intravascular administration can be manufactured such that a stem cell administered into a vein can stably reach a target tissue, and thus can more efficiently increase efficacy displaying activity, thereby innovatively enhancing the efficacy of cell therapy using stem cells.

25. 发明专利

ZA201407585B METHOD FOR MANUFACTURING STEM CELL HAVING APPROPRIATE SIZE FOR INTRAVASCULAR ADMINISTRATION 未知
公开(公告)号：ZA201407585B
公开(公告)日：2016-01-27
申请号：ZA201407585
申请日：2014-10-17
申请人： RA JEONG CHAN , K-STEMCELL CO LTD
发明人： KANG SUNG KEUN , SHIN II SEOB , RA JEONG CHAN
摘要： The present invention relates to a method for preparing stem cells having a size suitable for intravascular administration, and preferably to a method for preparing stem cells with a diameter ranging from 11-16 µm. Additionally, the present invention relates to media composition for preparing stem cells having a size suitable for intravascular administration. According to the present invention, stem cells having a size suitable for intravascular administration can be prepared such that stem cells administered into a vein can stably reach a target tissue, and thus can more efficiently increase efficacy displaying activity, thereby innovatively enhancing the efficacy of cell therapy using stem cells.

26. 发明专利

AU2013247455A1 Method and composition for preventing stem cell disruption and aggregation 未知
公开(公告)号：AU2013247455A1
公开(公告)日：2014-11-20
申请号：AU2013247455
申请日：2013-04-15
申请人： R BIO CO LTD , RA JEONG CHAN
发明人： RA JEONG CHAN , KANG SUNG KEUN , SHIN IL SEOB
IPC分类号： C12N5/074 , A61K35/12 , A61K35/28 , C12N5/0775
摘要： The present invention relates to a method for preventing stem cell disruption and aggregation. The present invention also relates to a stem cell composition for intravascular administration, in which stem cells are suspended in a solution containing aspirin. The present invention also relates to a composition for preventing stem cell disruption or aggregation, in which stem cells are suspending in a solution containing aspirin. According to the present invention, cell disruption and aggregation can be prevented during transport or storage, thus enabling the administered stem cells to stably reach target tissue and exhibit the activity thereof in a more efficient manner, thereby remarkably increasing the effects of cell therapy using stem cells.

27. 发明申请

WO2012115488A2 TEABAG SALT FOR COOKING 审中-公开
标题翻译： TEABAG盐烹饪
公开(公告)号：WO2012115488A2
公开(公告)日：2012-08-30
申请号：PCT/KR2012001454
申请日：2012-02-27
申请人： RNL SAMMI CO LTD , RA JEONG CHAN
发明人： RA JEONG CHAN
IPC分类号： A23L27/40 , B65D85/808
CPC分类号： B65D85/812 , A23L27/40 , B65D75/002
摘要： The present invention relates to teabag salt for cooking, and more particularly, to teabag salt for cooking, which is manufactured as a teabag so as to enable users to easily adjust the amount of salt to be added during cooking and prevent salt from flowing or spilling when carried. The teabag salt of the present invention may advantageously prevent users from adding too much salt during cooking and enable users to conveniently carry salt for outdoor activities such as mountain climbing, fishing, camping, or the like.
摘要翻译：用于烹饪的茶袋盐技术领域本发明涉及一种用于烹饪的茶袋盐，更具体地涉及一种作为茶袋制造的用于烹饪的茶袋盐，以使用户能够容易地调节在烹饪过程中加入的盐的量并防止盐流动或溢出携带时。本发明的茶袋盐可以有利地防止使用者在烹饪过程中添加太多的盐，并且使用户能够方便地携带用于诸如登山，钓鱼，野营等的户外活动的盐。

28. 发明专利

PT2247285E AGENTE TERAPÊUTICO CELULAR PARA INCONTINÊNCIA DE URINA COMPREENDENDO CÉLULAS ESTAMINAIS ORIGINÁRIAS DE DECÍDUA 未知
公开(公告)号：PT2247285E
公开(公告)日：2014-07-25
申请号：PT08854636
申请日：2008-12-01
申请人： RNL BIO CO LTD
发明人： RA JEONG CHAN , LEE HANG YOUNG , JO JUNG YOUN , KIM YUN JUNG
IPC分类号： A61K35/28 , A61K9/00 , A61K35/12 , A61K35/50 , A61P13/00 , C12N5/00

29. 发明专利

ES2478678T3 Agente terapéutico celular para la incontinencia urinaria que comprende células madre originadas a partir de decidua 未知
公开(公告)号：ES2478678T3
公开(公告)日：2014-07-22
申请号：ES08854636
申请日：2008-12-01
申请人： RNL BIO CO LTD
发明人： RA JEONG CHAN , LEE HANG YOUNG , JO JUNG YOUN , KIM YUN JUNG
IPC分类号： A61K35/28 , A61K9/00 , A61K35/12 , A61K35/50 , A61P13/00 , C12N5/00 , C12N5/074
摘要： Un agente terapéutico celular que contiene células madre derivadas de la decidua de la placenta o fluidos menstruales, para uso en el tratamiento de la incontinencia urinaria, en donde las células madre derivadas de la decidua de la placenta tienen las siguientes características: (a) muestran inmunofenotipos positivos para CD29 y CD90, pero negativos para CD31 y CD45; (b) muestran inmunofenotipos positivos para Oct4, SSEA-4 y Cripto-1; (c) se desarrollan fijadas a plástico, mostrando características morfológicas de forma redonda o de husillo, y formando esferas en medio SFM de manera que sean capaces de ser mantenidas en un estado no diferenciado durante un largo periodo de tiempo; y (d) tienen la capacidad de diferenciarse en miocitos.

30. 发明专利

CA2692634C METHOD FOR ISOLATING AND CULTURING ADULT STEM CELLS DERIVED FROM HUMAN AMNIOTIC EPITHELIUM 未知
公开(公告)号：CA2692634C
公开(公告)日：2014-05-27
申请号：CA2692634
申请日：2008-01-10
申请人： RNL BIO CO LTD
发明人： RA JEONG CHAN , SHIN IL SEOB , KANG SUNG KEUN , WOO SANG KYU
IPC分类号： C12N5/074 , A61K35/12 , A61K35/50 , C12N5/071 , C12N5/073
摘要： The present invention relates to a method for isolating and culturing adult stem cells derived from human amniotic membrane in high yield, and more particularly to a method for obtaining a large amount of adult stem cells, the method comprising obtaining amniotic epithelial cells from human amniotic tissue in high yield by treatment with dithiothreitol (DTT) and a low concentration of trypsin and culturing the amniotic epithelial cells in a medium containing a Rho-associated kinase inhibitor. The human amniotic epithelial cell-derived stem cells are easily extracted compared to existing therapeutic stem cells such as umbilical cord blood stem cells and bone marrow stem cells, the yield and proliferation thereof are significantly increased by DTT treatment, the addition of the ROCK inhibitor or the replacement of medium. Thus, the method can be used to efficiently prepare adult stem cells.

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