专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

21. 发明授权

US06756204B2 Probe composition comprising a binding polymer and polymer chain and methods of use 失效
标题翻译：包含结合聚合物和聚合物链的探针组合物及其使用方法
公开(公告)号：US06756204B2
公开(公告)日：2004-06-29
申请号：US10170044
申请日：2002-06-10
申请人： Paul David Grossman , Steven Fung , Steven Michael Menchen , Sam Lee Woo , Emily Susan Winn-Deen
发明人： Paul David Grossman , Steven Fung , Steven Michael Menchen , Sam Lee Woo , Emily Susan Winn-Deen
IPC分类号： C12Q168
CPC分类号： C12Q1/6816 , C12Q1/6825 , C12Q1/6827 , C12Q1/686 , C12Q1/6862 , C12Q2600/156 , G01N27/447 , Y10T436/143333 , C12Q2525/197 , C12Q2525/203 , C12Q2537/143 , C12Q2565/131 , C12Q2565/125 , C12Q2565/107 , C12Q2521/501 , C12Q2563/155 , C12Q2563/119 , C12Q2549/119
摘要： Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In the method, a mixture of sequence-specific probes are reacted with the target polynucleotide under hybridization conditions, and the hybridized probes are treated to selectively modify those probes which are bound to the target polynucleotide in a base-specific manner. The resulting labeled probes include a polymer chain which imparts to each different-sequence probe, a distinctive ratio of charge/translational frictional drag, and a detectable label. The labeled probes are fractionated by electrophoresis in a non-sieving matrix, and the presence of one or more selected sequences in the target polynucleotide are detected according to the observed electrophoretic migration rates of the labeled probes in a non-sieving medium.
摘要翻译：用于检测靶多核苷酸中的一个或多个选择的多核苷酸区域的方法和组合物。在该方法中，将序列特异性探针的混合物与靶多核苷酸在杂交条件下反应，并对杂交的探针进行处理以选择性地修饰以碱基特异性方式结合靶多核苷酸的那些探针。所得到的标记探针包括赋予每个不同序列探针的聚合物链，电荷/平移摩擦阻力的独特比例和可检测标记。标记的探针通过在非筛选基质中的电泳分级分离，并且根据在非筛选培养基中标记的探针的观察到的电泳迁移速率来检测靶多核苷酸中一个或多个选定序列的存在。

22. 发明授权

US5807682A Probe composition containing a binding domain and polymer chain and method of use 失效
标题翻译：含有结合域和聚合物链的探针组合物及其使用方法
公开(公告)号：US5807682A
公开(公告)日：1998-09-15
申请号：US877460
申请日：1997-06-17
申请人： Paul David Grossman , Steven Fung , Steven Michael Menchen , Sam Lee Woo , Emily Susan Winn-Deen
发明人： Paul David Grossman , Steven Fung , Steven Michael Menchen , Sam Lee Woo , Emily Susan Winn-Deen
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6816 , C12Q1/6825 , C12Q1/6827 , C12Q1/686 , C12Q1/6862 , C12Q2600/156 , G01N27/447 , Y10T436/143333
摘要： Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In the method, a mixture of sequence-specific probes are reacted with the target polynucleotide under hybridization conditions, and the hybridized probes are treated to selectively modify those probes which are bound to the target polynucleotide in a base-specific manner. The resulting labeled probes include a polymer chain which imparts to each different-sequence probe, a distinctive ratio of charge/translational frictional drag, and a detectable label. The labeled probes are fractionated by electrophoresis in a non-sieving matrix, and the presence of one or more selected sequences in the target polynucleotide are detected according to the observed electrophoretic migration rates of the labeled probes in a non-sieving medium.
摘要翻译：用于检测靶多核苷酸中的一个或多个选择的多核苷酸区域的方法和组合物。在该方法中，将序列特异性探针的混合物与靶多核苷酸在杂交条件下反应，并对杂交的探针进行处理以选择性地修饰以碱基特异性方式结合靶多核苷酸的那些探针。所得到的标记探针包括赋予每个不同序列探针的聚合物链，电荷/平移摩擦阻力的独特比例和可检测标记。标记的探针通过在非筛选基质中的电泳分级分离，并且根据在非筛选培养基中标记的探针的观察到的电泳迁移速率来检测靶多核苷酸中一个或多个选定序列的存在。

23. 发明授权

US5552471A Solid support reagents for the synthesis of 3'-Nitrogen containing polynucleotides 失效
标题翻译：用于合成含3'-氮的多核苷酸的固体支持试剂
公开(公告)号：US5552471A
公开(公告)日：1996-09-03
申请号：US293637
申请日：1994-08-17
申请人： Sam L. Woo , Steven Fung
发明人： Sam L. Woo , Steven Fung
IPC分类号： C12N15/09 , C07C217/20 , C07C271/10 , C07C271/16 , C07C271/66 , C07C317/18 , C07C333/04 , C07H21/00 , C07H21/04 , C03C12/00 , C08L25/06
CPC分类号： C07H21/00 , Y02P20/55
摘要： The compounds are exemplified by the class of sulfoethyl oxygen-substituted carbamates, such compounds being useful as support reagents for automated polynucleotide synthesis of 3'-nitrogen functionalized polynucleotides. The invention includes, in one aspect, a polynucleotide synthesis reagent having the structure: ##STR1## where T is an acid-cleavable hydroxyl protecting group, e.g., 4,4'-dimethoxytritil; Q is a linker connecting the nitrogen and oxygen, e.g., n-hexyl; R.sub.1 is a nitrogen substituent, e.g., hydrogen; R.sub.2 through R.sub.4 are separately hydrogen or lower alkyl; Y is an atom which is electronegative with respect to carbon, e.g., oxygen or sulfur; X.sub.1 is an atom which is electronegative with respect to carbon, e.g., sulfone; Z is a bond or spacer arm, e.g., ethylsuccinate; and W is a derivatized solid synthesis support capable of linking to Z, e.g., an amino-dirivitized controlled pore glass. In a second aspect, the present invention includes a polynucleotide synthesis reagent having the structure: ##STR2## where T, Q, R.sub.1, R.sub.2 -R.sub.3, X.sub.2, Z, and W are as defined above.
摘要翻译：所述化合物的例子是磺乙氧基取代的氨基甲酸酯类，这些化合物可用作3'-氮官能化多核苷酸的自动多核苷酸合成的载体试剂。在一个方面，本发明包括具有以下结构的多核苷酸合成试剂：其中T是酸可裂解的羟基保护基，例如4,4'-二甲氧基苦味; Q是连接氮和氧的接头，例如正己基; R1是氮取代基，例如氢; R2至R4分别为氢或低级烷基; Y是相对于碳例如氧或硫具有电负性的原子; X1是相对于碳电负性的原子，例如砜; Z是键或间隔臂，例如乙基琥珀酸盐; 并且W是能够与Z连接的衍生的固体合成载体，例如氨基 - 二硝基化的受控孔玻璃。在第二方面，本发明包括具有以下结构的多核苷酸合成试剂：其中T，Q，R 1，R 2，R 3，X 2，Z和W如上所定义。

24. 发明授权

US5514543A Method and probe composition for detecting multiple sequences in a single assay 失效
标题翻译：用于在单个测定中检测多个序列的方法和探针组合物
公开(公告)号：US5514543A
公开(公告)日：1996-05-07
申请号：US102372
申请日：1993-08-04
申请人： Paul D. Grossman , Steven Fung , Steven M. Menchen , Sam L. Woo , Emily S. Win-Deen
发明人： Paul D. Grossman , Steven Fung , Steven M. Menchen , Sam L. Woo , Emily S. Win-Deen
IPC分类号： C12N15/09 , B01D57/02 , C12Q1/68 , G01N27/447 , G01N30/88 , G01N33/566
CPC分类号： C12Q1/6869 , C12Q1/6816 , C12Q1/6827 , C12Q1/6858 , C12Q1/686 , C12Q1/6862 , C12Q1/6818 , Y10T436/143333
摘要： Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In one embodiment of the invention, a plurality of different-sequence probe pairs are added to a target polynucleotide, where each probe pair includes two polynucleotide probe elements which are complementary in sequence to adjacent portions of a selected one of the target sequences in the target polynucleotide. In each probe pair, one of the probe elements contains a non-polynucleotide polymer chain which imparts a distinctive mobility to the associated probe pair, when the elements in the pair are ligated. The other element in the pair contains a detectable reporter label. After the probe pairs have been allowed to hybridize with the target polynucleotide, the hybridized polynucleotides are treated under conditions effective to ligate the end subunits of target-bound probe elements when their end subunits are base-paired with adjacent target bases. The ligated probe pairs are then released from the target polynucleotide and separated electrophoretically in a sieving matrix, or chromatographically.
摘要翻译：用于检测靶多核苷酸中的一个或多个选择的多核苷酸区域的方法和组合物。在本发明的一个实施方案中，将多个不同序列探针对加入到靶多核苷酸中，其中每个探针对包含两个多核苷酸探针元件，该多核苷酸探针元件与目标中所选择的一个目标序列的相邻部分互补多核苷酸。在每个探针对中，探针元件中的一个包含非多核苷酸聚合物链，当对中的元件被连接时，赋予相关探针对的特异性移动性。该对中的另一个元素包含可检测的报告物标记。在探针对已被允许与靶多核苷酸杂交后，杂交的多核苷酸在有效连接靶结合探针元件的末端亚基的条件下处理，当它们的末端亚基与相邻的靶基碱基配对时。然后将连接的探针对从目标多核苷酸中释放，并在筛分基质中电泳分离，或进行色谱分离。

25. 发明授权

US5366860A Spectrally resolvable rhodamine dyes for nucleic acid sequence determination 失效
标题翻译：用于核酸序列测定的光谱可分辨的罗丹明染料
公开(公告)号：US5366860A
公开(公告)日：1994-11-22
申请号：US415050
申请日：1989-09-29
申请人： B. John Bergot , Vergine Chakerian , Charles R. Connell , J. Scott Eadie , Steven Fung , N. Davis Hershey , Linda G. Lee , Steven M. Menchen , Sam L. Woo
发明人： B. John Bergot , Vergine Chakerian , Charles R. Connell , J. Scott Eadie , Steven Fung , N. Davis Hershey , Linda G. Lee , Steven M. Menchen , Sam L. Woo
IPC分类号： C09B69/10 , C07H19/10 , C07H19/20 , C07H21/00 , C09B11/24 , C12Q1/68
CPC分类号： C12Q1/6869 , C07H21/00 , C09B11/24
摘要： A spectrally resolvable set of rhodamine dyes are provided for use in the chain termination method of nucleic acid sequencing. A different rhodamine dye from the group consisting of tetramethylrhodamine, rhodamine X, rhodamine 6G, and rhodamine 110 is attached to the base of each of the dideoxynucleotides used in the sequencing method by way of an alkynylamino linker. Preferably, the labeled dideoxynucleotides are incorporated into the growing DNA chains by Taq DNA polymerase.
摘要翻译：提供光谱可分辨的罗丹明染料组合用于核酸测序的链终止方法。通过炔基氨基接头将与四氯乙酰胺，罗丹明X，罗丹明6G和罗丹明110组成的组中的不同的罗丹明染料连接到用于测序方法的每个双脱氧核苷酸的碱基上。优选地，标记的双脱氧核苷酸通过Taq DNA聚合酶掺入生长的DNA链中。

26. 发明授权

US4855225A Method of detecting electrophoretically separated oligonucleotides 失效
标题翻译：检测电泳分离的寡核苷酸的方法
公开(公告)号：US4855225A
公开(公告)日：1989-08-08
申请号：US827348
申请日：1986-02-07
申请人： Steven Fung , Sam L. Woo , Richard P. Haugland , Steven M. Menchen , Charles R. Connell
发明人： Steven Fung , Sam L. Woo , Richard P. Haugland , Steven M. Menchen , Charles R. Connell
IPC分类号： C07H21/04 , C07H1/06 , C07H21/00 , C12Q1/68 , G01N27/26 , G01N27/447 , G01N33/50 , G01N33/58
CPC分类号： G01N33/582 , C07H21/00 , C12Q1/6816 , C12Q1/6874
摘要： A method is provided for detecting up to four classes of oligonucleotides which have been separated by gel electrophoresis. The method entails labeling members of each class of oligonucleotide with dyes selected from separate sets of dyes so that members of the same class are labeled with dyes from the same set. The four sets of dyes of the invention consist of derivatives of fluorescein, 2',7'-dimethoxy-4', 5'-dichlorofluorescein, tetramethylrhodamine, and rhodamine X carboxylic or sulfonic acid, respectively. Dyes from these sets are spectrally resolvable under conditions of gel electrophoresis.
摘要翻译：提供了一种用于检测多达四类通过凝胶电泳分离的寡核苷酸的方法。该方法需要用选自单独的染料组的染料标记每一类寡核苷酸的成员，使得同一类的成员用来自同一组的染料标记。本发明的四组染料分别由荧光素，2'，7'-二甲氧基-4'，5'-二氯荧光素，四甲基罗丹明和罗丹明X羧酸或磺酸的衍生物组成。来自这些组的染料在凝胶电泳条件下是光谱可分辨的。

27. 发明授权

US4408077A 6-(Lower alkoxy)-5-(trifluoromethyl)-1-naphthalenecarboxaldehydes 失效
标题翻译： 6-（低级烷氧基）-5-（三氟甲基）-1-萘甲醛
公开(公告)号：US4408077A
公开(公告)日：1983-10-04
申请号：US321302
申请日：1981-11-13
申请人： Kazimir Sestanj , Steven Fung , Nedumparambil A. Abraham , Francesco Bellini
发明人： Kazimir Sestanj , Steven Fung , Nedumparambil A. Abraham , Francesco Bellini
IPC分类号： C07C17/26 , C07C29/58 , C07C37/06 , C07C43/225 , C07C45/00 , C07C45/42 , C07C47/575 , C07C49/223 , C07C47/115
CPC分类号： C07C17/2632 , C07C29/58 , C07C37/07 , C07C43/225 , C07C45/004 , C07C45/42 , C07C47/575 , C07C49/223
摘要： A process and intermediates for preparing 6-(lower alkoxy)-5-(trifluoromethyl)-1-naphthalenecarboxylic acid derivatives are disclosed. The derivatives are useful for preparing aldose reductase inhibitors. With reference to the process, 1,1,1-trifluoro-5-(2-methylphenyl)-2,3-pentadione 3-oxime is cyclized to give a key intermediate 3,4-dihydro-1-hydroxy-5-methyl-1-(trifluoromethyl)-2(1H)-naphthalenone oxime; and 3,4-dihydro-1-hydroxy-5-methyl-1-(trifluoromethyl)-2(1H)-naphthalenone is aromatized to 5-methyl-1-(trifluoromethyl)-2-naphthalenol with a dehydrating agent.
摘要翻译：公开了制备6-（低级烷氧基）-5-（三氟甲基）-1-萘甲酸衍生物的方法和中间体。该衍生物可用于制备醛糖还原酶抑制剂。参考该方法，将1,1,1-三氟-5-（2-甲基苯基）-2,3-戊二酮3-肟环化，得到关键中间体3,4-二氢-1-羟基-5-甲基 -1-（三氟甲基）-2（1H） - 萘酮肟; 和3,4-二氢-1-羟基-5-甲基-1-（三氟甲基）-2（1H） - 萘酮用脱水剂芳构化成5-甲基-1-（三氟甲基）-2-萘酚。

28. 发明授权

US08703497B2 Nanowire-based system for analysis of nucleic acids 有权
标题翻译：基于纳米线的核酸分析系统
公开(公告)号：US08703497B2
公开(公告)日：2014-04-22
申请号：US13487638
申请日：2012-06-04
申请人： Hongye Sun , Steven Fung , Sam Woo
发明人： Hongye Sun , Steven Fung , Sam Woo
IPC分类号： G01N27/00 , G01N27/403 , C12M1/34 , B82Y15/00 , G01N27/04 , G01N27/22
CPC分类号： C12Q1/6874 , C12Q1/6811 , C12Q1/6869 , G01N27/3276 , G01N27/3278 , Y10T436/14 , Y10T436/142222 , Y10T436/143333 , C12Q2565/607 , C12Q2533/101 , C12Q2565/133 , C12Q2563/155
摘要： A system and method for analyzing nucleic acids is presented herein. The system can include a nanowire assembly including a plurality of nanowires. In some embodiments, at least two nanowires can be coupled to a different probe corresponding to a different sequence version of a polymorphism. The system can include a reagent delivery system capable of delivering a template polymorphic nucleotide, an extension nucleic acid, and an enzyme to the nanowire assembly. The system can include an electrical detector configured to measure an electrical characteristic of the nanowire assembly and/or a controller in communication with the reagent delivery system. In some embodiments, the electrical detector can be configured to collect data corresponding to changes in the electrical characteristic in response to contact with the template polymorphic nucleotide, the extension nucleic acid, and the enzyme.
摘要翻译：本文提供了用于分析核酸的系统和方法。该系统可以包括包括多个纳米线的纳米线组件。在一些实施例中，至少两个纳米线可以耦合到对应于多态性的不同序列形式的不同探针。该系统可以包括能够将模板多核苷酸，延伸核酸和酶递送至纳米线组件的试剂递送系统。该系统可以包括被配置为测量纳米线组件和/或与试剂递送系统连通的控制器的电特性的电检测器。在一些实施方案中，电检测器可被配置为响应于与模板多核苷酸，延伸核酸和酶的接触而收集对应于电特性变化的数据。

29. 发明申请

US20120244537A1 Nanowire-Based System for Analysis of Nucleic Acids 有权
标题翻译：基于纳米线的核酸分析系统
公开(公告)号：US20120244537A1
公开(公告)日：2012-09-27
申请号：US13487638
申请日：2012-06-04
申请人： Hongye Sun , Steven Fung , Sam Lee Woo
发明人： Hongye Sun , Steven Fung , Sam Lee Woo
IPC分类号： G01N27/00 , G01N27/22 , C12M1/34 , G01N27/04 , B82Y15/00
CPC分类号： C12Q1/6874 , C12Q1/6811 , C12Q1/6869 , G01N27/3276 , G01N27/3278 , Y10T436/14 , Y10T436/142222 , Y10T436/143333 , C12Q2565/607 , C12Q2533/101 , C12Q2565/133 , C12Q2563/155
摘要： System for detection and/or analysis of nucleic acids using nanowires to detect covalent modification of nucleic acids.
摘要翻译：用于使用纳米线检测和/或分析核酸以检测核酸共价修饰的系统。

30. 发明申请

US20070238186A1 Nanowire-based system for analysis of nucleic acids 审中-公开
标题翻译：基于纳米线的核酸分析系统
公开(公告)号：US20070238186A1
公开(公告)日：2007-10-11
申请号：US11394147
申请日：2006-03-29
申请人： Hongye Sun , Steven Fung , Sam Woo
发明人： Hongye Sun , Steven Fung , Sam Woo
IPC分类号： G01N33/00 , G08B7/06
CPC分类号： C12Q1/6874 , C12Q1/6811 , C12Q1/6869 , G01N27/3276 , G01N27/3278 , Y10T436/14 , Y10T436/142222 , Y10T436/143333 , C12Q2565/607 , C12Q2533/101 , C12Q2565/133 , C12Q2563/155
摘要： System for detection and/or analysis of nucleic acids using nanowires to detect covalent modification of nucleic acids.
摘要翻译：用于使用纳米线检测和/或分析核酸以检测核酸共价修饰的系统。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式