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21. 发明授权

US4317882A Production of plasminogen activator 失效
标题翻译：生产纤溶酶原激活物
公开(公告)号：US4317882A
公开(公告)日：1982-03-02
申请号：US143680
申请日：1980-04-25
申请人： Sadayuki Horiguchi , Akio Hasegawa , Mitsuru Shibukawa
发明人： Sadayuki Horiguchi , Akio Hasegawa , Mitsuru Shibukawa
IPC分类号： A61K38/00 , C12N9/72 , C12N9/48
CPC分类号： C12N9/6462 , C12Y304/21073 , A61K38/00 , Y10S435/818
摘要： A plasminogen activator having a molecular weight of 45,000 to 68,000 is formed as a single fraction in the cells of human kidney or lung, and is separated and recovered with good efficiency without reducing its molecular weight. The pH of a solution to be contacted with said cells and the concentration of dissolved oxygen should be maintained within suitable ranges in order to form the plasminogen activator having a molecular weight of 45,000 to 68,000 as a single fraction. Furthermore, by properly adjusting the residence time of the solution with the cells, the activator can be formed over a long period of more than 40 days. To separate the activator, the pH of the solution in the separating step is maintained preferably at 4 to 12. By causing a metal chelating agent to be present in the solution in the separating step, the plasminogen activator can be obtained without reducing its molecular weight.
摘要翻译：在人肾或肺细胞中形成分子量为45,000至68,000的纤溶酶原激活剂作为单一部分，并且在不降低其分子量的情况下以高效率分离和回收。要与所述细胞接触的溶液的pH值和溶解氧的浓度应保持在合适的范围内，以形成分子量为45,000至68,000的纤溶酶原激活剂作为单一部分。此外，通过适当调整溶液与细胞的停留时间，可以在40天以上的长时间内形成活化剂。为了分离活化剂，分离步骤中的溶液的pH优选保持在4至12.通过在分离步骤中使溶液中存在金属螯合剂，可以在不降低其分子量的情况下获得纤溶酶原激活剂。

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