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    • 29. 发明申请
    • COMPOSITIONS AND METHODS FOR AUXIN-ANALOG CONJUGATION
    • 用于辅助模拟结合的组合物和方法
    • WO2014100525A3
    • 2014-09-04
    • PCT/US2013076771
    • 2013-12-20
    • PIONEER HI BRED INT
    • CASTLE LINDA ACHIU LI-WEITAO YUMIN
    • C12N15/82
    • A01N37/38A01N37/10C12N9/2402C12N15/8274C12N15/8294C12Y302/01
    • Compositions and methods are provided to detoxify an auxin-analog herbicide through the use of at least one GH3 polypeptide having amino acid/auxin analog herbicide conjugation activity. Such GH3 polypeptides in the presence of an auxin-analog herbicide will produce an amino acid/auxin-analog conjugate having reduced herbicidal activity. Various methods of employing the GH3 polypeptides and the polynucleotides encoding the same are provided. Such methods include methods to detoxify an auxin-analog herbicide comprising applying to a plant, a plant cell or a seed an auxin-analog herbicide, wherein the plant, plant cell or seed comprises a heterologous polynucleotide encoding a GH3 polypeptide, and expression of the GH3 polypeptide produces a non-herbicidal aspartate/auxin-analog conjugate or a glutamate/auxin-analog conjugate. Additional methods for controlling at least one weed in an area of cultivation comprising a crop or a seed of the crop are provided, as are methods of detoxifying a contaminated material having an auxin-analog herbicide.
    • 提供组合物和方法以通过使用至少一种具有氨基酸/生长素类似物除草剂共轭活性的GH3多肽来解毒生长素类似物除草剂。 在生长素类似物除草剂存在下,这样的GH3多肽将产生具有降低的除草活性的氨基酸/生长素 - 类似物结合物。 提供了使用GH3多肽和编码其的多核苷酸的各种方法。 这样的方法包括对生长素类似物除草剂进行解毒的方法,其包括向植物,植物细胞或种子施用生长素类似物除草剂,其中植物,植物细胞或种子包含编码GH3多肽的异源多核苷酸, GH3多肽产生非除草天冬氨酸/生长素 - 类似物结合物或谷氨酸/生长素 - 类似物缀合物。 提供了用于控制包含作物的作物或种子的栽培区域中的至少一种杂草的附加方法,以及对具有生长素类似物除草剂的污染物质进行解毒的方法。