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    • 24. 发明公开
    • POLYPEPTIDES WITH LIPASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME
    • 多巴胺多巴胺多巴胺多甙
    • EP2976416A1
    • 2016-01-27
    • EP14710916.9
    • 2014-03-19
    • Novozymes A/S
    • MORANT, Marc DominiqueREISER, Anna VerenaHANSEN, Carsten HoerslevBORCH, KimBAUNSGAARD, LoneBHATIA, Vikram Kjoeller
    • C11D3/386C12N9/20
    • C11D3/38627C11D3/38681C12N9/20C12P13/008
    • The present invention relates to isolated polypeptides with lipase activity, selected from the group consisting of: (a) a polypeptide having at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 4; (b) a polypeptide encoded by a polynucleotide that hybridizes under low stringency conditions, medium stringency conditions, medium-high stringency conditions, high stringency conditions, or very high stringency conditions with (i) SEQ ID NO: 3 or the full-length complement of (i); (c) a polypeptide encoded by a polynucleotide having at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 3; (d) a polypeptide which is a variant of SEQ ID NO: 4 comprising a substitution, deletion, and/or insertion at one or more (e.g. several) positions; and (e) a polypeptide which is a fragment of any of the polypeptides of (a), (b), (c) or (d). The invention also relates to polynucleotides encoding the polypeptides; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the polypeptides.
    • 本发明涉及具有脂肪酶活性的分离的多肽,其选自:(a)多肽,其具有至少75%,至少80%,至少85%,至少90%,至少91% 与SEQ ID NO:4的至少92%,至少93%,至少94%,至少95%,至少96%,至少97%,至少98%,至少99%或100%的序列同一性 ; (b)由低度严格条件,中等严格条件,中等严格条件,高严格条件或非常高严格条件与(i)SEQ ID NO:3或全长补体 的(i); (c)由多核苷酸编码的多肽,所述多核苷酸具有至少75%,至少80%,至少85%,至少90%,至少91%,至少92%,至少93%,至少94% 与SEQ ID NO:3的至少95%,至少96%,至少97%,至少98%,至少99%或100%的序列同一性; (d)在一个或多个(例如几个)位置上包含取代,缺失和/或插入的SEQ ID NO:4的变体的多肽; 和(e)作为(a),(b),(c)或(d)的多肽的片段的多肽。 本发明还涉及编码多肽的多核苷酸; 核酸构建体,载体和包含多核苷酸的宿主细胞; 和使用多肽的方法。