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21. 发明申请

US20130065831A1 METHOD FOR THE PRODUCTION OF CONJUGATES OF INSULIN-LIKE GROWTH FACTOR-1 AND POLY(ETHYLENE GLYCOL) 有权
标题翻译：产生胰岛素样生长因子-1和聚（乙二醇）的方法
公开(公告)号：US20130065831A1
公开(公告)日：2013-03-14
申请号：US13590549
申请日：2012-08-21
申请人： Stephan Fischer , Friederike Hesse , Hendrik Knoetgen , Kurt Lang , Friedrich Metzger , Joerg Thomas Regula , Christian Schantz , Andreas Schaubmar , Hans-Joachim Schoenfeld
发明人： Stephan Fischer , Friederike Hesse , Hendrik Knoetgen , Kurt Lang , Friedrich Metzger , Joerg Thomas Regula , Christian Schantz , Andreas Schaubmar , Hans-Joachim Schoenfeld
IPC分类号： A61K38/30 , A61P25/28
CPC分类号： C07K14/65 , A61K47/60 , C07K2319/21 , C07K2319/50
摘要： The present invention relates to a fusion protein comprising IGF-I or an IGF-I variant N-terminally linked to the C-terminus of a propeptide. The invention relates also to a method involving the use of the aforementioned fusion protein in the production of a lysine-PEGylated IGF-I or IGF-I variant. The method comprises the steps of cultivating a prokaryotic host cell comprising an expression vector containing a nucleic acid encoding the fusion protein and causing the cell to express the fusion protein, recovering and PEGylating said fusion protein, cleaving said PEGylated fusion protein with IgA protease, and recovering lysine-PEGylated IGF-I or IGF-I variant. The invention relates also to a lysine-PEGylated IGF-I or IGF-I variant produced using the above method. In addition, the invention relates to a method for treating a neurodegenerative disorders like Alzheimer's Disease using the lysine-PEGylated IGF-I or IGF-I variant and a composition comprising the lysine-PEGylated IGF-I or IGF-I variant.
摘要翻译：本发明涉及包含IGF-I或与前肽的C-末端N末端连接的IGF-1变体的融合蛋白。本发明还涉及涉及在制备赖氨酸 - PEG化IGF-I或IGF-I变体中使用上述融合蛋白的方法。该方法包括以下步骤：培养包含含有编码融合蛋白的核酸的表达载体的原核宿主细胞，并使细胞表达融合蛋白，回收聚乙二醇化所述融合蛋白，用IgA蛋白酶切割所述聚乙二醇化融合蛋白;以及回收赖氨酸 - PEG化IGF-I或IGF-I变体。本发明还涉及使用上述方法制备的赖氨酸 - 聚乙二醇化IGF-I或IGF-I变体。此外，本发明涉及使用赖氨酸PEG化IGF-I或IGF-1变体和包含赖氨酸 - PEG化IGF-I或IGF-I变体的组合物治疗神经变性疾病如阿尔茨海默病的方法。

22. 发明申请

US20130011394A1 COMPLEXES COMPRISING MHC CLASS I FUSION POLYPEPTIDES AND ANTIGEN-SPECIFIC ANTIBODIES AND METHODS OF USE 审中-公开
标题翻译：包含MHC类I融合多聚体和抗原特异性抗体的复合物及其使用方法
公开(公告)号：US20130011394A1
公开(公告)日：2013-01-10
申请号：US13526718
申请日：2012-06-19
申请人： Hendrik Knoetgen
发明人： Hendrik Knoetgen
IPC分类号： A61K39/395 , C07K19/00 , A61K39/42 , A61P35/00 , A61P31/12 , C12N5/10 , C12P21/02 , C12N15/62
摘要： The invention comprises a complex comprising as first part an antibody derived part that specifically binds to a target antigen, and as second part a virus-derived peptide linked to a MHC class I protein complex.
摘要翻译：本发明包括复合物，其包含与靶抗原特异性结合的抗体衍生部分的第一部分，以及作为第二部分与MHC I类蛋白复合物连接的病毒衍生肽。

23. 发明申请

US20100249379A1 PROTEIN EXPRESSION FROM MULTIPLE NUCLEIC ACIDS 有权
标题翻译：来自多种核酸的蛋白质表达
公开(公告)号：US20100249379A1
公开(公告)日：2010-09-30
申请号：US12681781
申请日：2008-10-09
申请人： Ulrich Goepfert , Hendrik Knoetgen , Erhard Kopetzki , Anne Stern
发明人： Ulrich Goepfert , Hendrik Knoetgen , Erhard Kopetzki , Anne Stern
IPC分类号： C07K16/28 , C12P21/08
CPC分类号： C12N15/85 , C07K16/18 , C07K16/28 , C07K16/2812 , C07K16/2854 , C07K16/2866 , C07K16/2896 , C07K2317/14 , C07K2317/21 , C07K2317/51 , C07K2317/515 , C12P21/00
摘要： The current invention reports a method for the recombinant production of a secreted heterologous immunoglobulin in a CHO cell comprising the following steps: i) providing a CHO cell, which is adapted to growth in suspension culture, adapted to growth in serum-free medium, mycoplasma free, and virus free, ii) providing a vector comprising a prokaryotic origin of replication, a first nucleic acid conferring resistance to a prokaryotic selection agent, a second nucleic acid encoding the heavy chain of said heterologous immunoglobulin, a third nucleic acid encoding the light chain of said heterologous immunoglobulin, a fourth nucleic acid conferring resistance to a eukaryotic selection agent, iii) transfecting said CHO cell, wherein said transfecting comprises a) transfecting said CHO cell with said vector comprising a fourth nucleic acid conferring resistance to a first eukaryotic selection agent, b) selecting a CHO cell by growth in cultivation medium containing said first eukaryotic selection agent, c) transfecting said selected CHO cell with said vector comprising a fourth nucleic acid conferring resistance to a second eukaryotic selection agent different to said first eukaryotic selection agent, d) selecting a CHO cell by selected growth in cultivation medium containing said first and said second eukaryotic selection agent, iv) cultivating said transfected CHO cell in a medium in the presence of said first and second eukaryotic selection agent, under conditions suitable for the expression of said second, and third nucleic acid, and v) recovering said secreted heterologous immunoglobulin from the cultivation medium.
摘要翻译：本发明报告了在CHO细胞中重组产生分泌的异源免疫球蛋白的方法，其包括以下步骤：i）提供适于在悬浮培养物中生长的CHO细胞，适于在无血清培养基中生长，支原体免费和无病毒，ii）提供包含原核复制起点的载体，赋予原核选择剂抗性的第一核酸，编码所述异源免疫球蛋白的重链的第二核酸，编码光的第三核酸所述异源免疫球蛋白链，赋予对真核选择剂的抗性的第四核酸，iii）转染所述CHO细胞，其中所述转染包括a）用包含第四核酸的所述载体转染所述CHO细胞，所述第四核酸赋予对第一真核选择性的抗性试剂，b）通过在含有所述第一种真核生物的培养基中生长来选择CHO细胞 c）用所述载体转染所述选择的CHO细胞，所述载体包含赋予与所述第一真核选择试剂不同的第二真核选择剂的抗性的第四核酸，d）通过在含有所述第一和第二真核选择试剂的培养基中选择生长来选择CHO细胞，所述第二真核选择剂，iv）在所述第一和第二真核选择剂的存在下，在适于表达所述第二和第三核酸的条件下，在培养基中培养所述转染的CHO细胞，和v）回收所述分泌的异源免疫球蛋白。

24. 发明申请

US20100210547A1 METHOD FOR THE PRODUCTION OF CONJUGATES OF INSULIN-LIKE GROWTH FACTOR-1 AND POLY(ETHYLENE GLYCOL) 审中-公开
公开(公告)号：US20100210547A1
公开(公告)日：2010-08-19
申请号：US12767829
申请日：2010-04-27
申请人： Stephan Fischer , Friederike Hesse , Hendrik Knoetgen , Kurt Lang , Friedrich Metzger , Joerg Thomas Regula , Christian Schantz , Andreas Schaubmar , Hans-Joachim Schoenfeld
发明人： Stephan Fischer , Friederike Hesse , Hendrik Knoetgen , Kurt Lang , Friedrich Metzger , Joerg Thomas Regula , Christian Schantz , Andreas Schaubmar , Hans-Joachim Schoenfeld
IPC分类号： A61K38/18 , A61P25/28
CPC分类号： C07K14/65 , A61K47/60 , C07K2319/21 , C07K2319/50
摘要： The present invention relates to a fusion protein comprising IGF-I or an IGF-I variant N-terminally linked to the C-terminus of a propeptide. The invention relates also to a method involving the use of the aforementioned fusion protein in the production of a lysine-PEGylated IGF-I or IGF-I variant. The method comprises the steps of cultivating a prokaryotic host cell comprising an expression vector containing a nucleic acid encoding the fusion protein and causing the cell to express the fusion protein, recovering and PEGylating said fusion protein, cleaving said PEGylated fusion protein with IgA protease, and recovering lysine-PEGylated IGF-I or IGF-I variant. The invention relates also to a lysine-PEGylated IGF-I or IGF-I variant produced using the above method. In addition, the invention relates to a method for treating a neurodegenerative disorders like Alzheimer's Disease using the lysine-PEGylated IGF-I or IGF-I variant and a composition comprising the lysine-PEGylated IGF-I or IGF-I variant.

25. 发明申请

US20100184144A1 IMMUNOGLOBULIN PRODUCTION 有权
标题翻译：免疫球蛋白生产
公开(公告)号：US20100184144A1
公开(公告)日：2010-07-22
申请号：US12664401
申请日：2008-06-25
申请人： Ulrich Goepfert , Silke Hansen , Hendrik Knoetgen , Erhard Kopetzki , Oliver Ploettner
发明人： Ulrich Goepfert , Silke Hansen , Hendrik Knoetgen , Erhard Kopetzki , Oliver Ploettner
IPC分类号： C12P21/00 , C07H21/00 , C12N15/74 , C12N5/10
CPC分类号： C07K16/00 , C07K2317/21 , C07K2317/52 , C12N2830/42
摘要： The current invention comprises a nucleic acid encoding the amino acid sequence of the C-terminal part of the CH3-domain of an immunoglobulin of the class IgA or IgG, or the C-terminal part of the CH4-domain of an immunoglobulin of the class IgE or IgM, wherein the glycine-lysine-dipeptide comprised in the amino acid sequence of the C-terminal part of the CH3- or CH4-domain is encoded by the nucleic acid ggaaaa, or the nucleic acid ggcaaa, or the nucleic acid gggaaa, or the nucleic acid gggaag, or the nucleic acid ggcaag, or the nucleic acid ggaaag.
摘要翻译：本发明包括编码IgA或IgG类免疫球蛋白的CH3结构域的C末端部分的氨基酸序列的核酸，或类别的免疫球蛋白的CH4结构域的C末端部分 IgE或IgM，其中包含在CH 3 - 或CH 4结构域的C-末端部分的氨基酸序列中的甘氨酸 - 赖氨酸二肽由核酸ggaaaa或核酸ggcaaa或核酸gggaaa ，或核酸gggaag，或核酸ggcaag，或核酸ggaaag。

26. 发明申请

US20100080812A1 Antibodies against human IL17 and uses thereof 有权
标题翻译：针对人IL17的抗体及其用途
公开(公告)号：US20100080812A1
公开(公告)日：2010-04-01
申请号：US12586893
申请日：2009-09-29
申请人： Johannes Auer , Nikolaos Dimoudis , Guy Georges , Petra Hanke , Hendrik Knoetgen , Ekkehard Moessner , Claire Louise Langrish
发明人： Johannes Auer , Nikolaos Dimoudis , Guy Georges , Petra Hanke , Hendrik Knoetgen , Ekkehard Moessner , Claire Louise Langrish
IPC分类号： A61K39/395 , C07K16/00 , C07H21/00 , A61P11/06 , A61P19/02 , C12N15/63 , C12P21/00 , C12N5/00
CPC分类号： C07K16/244 , C07K2317/76 , Y10S530/809
摘要： An antibody binding to IL-17 characterized by binding to the same IL-17 epitope to which monoclonal antibody 3C1 binds, and being of human IgG1 isotype modified in the hinge region at amino acid position 216-240, preferably at amino acid position 220-240, between CH1 and CH2 and/or in the second inter-domain region at amino acid position 327-331 between CH2 and CH3 has advantageous properties for the treatment of inflammatory diseases.
摘要翻译：结合IL-17的抗体，其特征在于与单克隆抗体3C1结合的相同的IL-17表位结合，并且在氨基酸位置216-240，优选氨基酸位置220-位于铰链区修饰的人IgG1同种型， 240，CH1和CH2之间和/或在CH2和CH3之间的氨基酸位置327-331的第二区域间区域具有治疗炎性疾病的有利性质。

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