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    • 24. 发明授权
    • Method for the production of biopolymers with modified properties
    • 生产具有改性特性的生物聚合物的方法
    • US06821758B1
    • 2004-11-23
    • US09708497
    • 2000-11-09
    • Andre KoltermannUlrich KettlingManfred Eigen
    • Andre KoltermannUlrich KettlingManfred Eigen
    • C12P1934
    • C12N15/1027
    • A method for the production of biopolymers with modified properties wherein at least one cycle comprising the following steps is completed: (a) providing a population of single-stranded polynucleotide molecules, wherein individual polynucleotide molecules comprise homologous and heterologous sequence segments and wherein individual ones of said single-stranded polynucleotide molecules can form double-stranded polynucleotide molecules with other ones of said single-stranded polynucleotide molecules within said population; (b) forming double-stranded polynucleotide molecules from the population of single-stranded polynucleotide molecules provided according to step (a) comprising double-stranded polynucleotide molecules with different heterologous sequence segments; (c) partially and exonucleolytically degrading the single-strands of the double-stranded polynucleotide molecules produced according to step (b); and (d) temple-directed single-stand synthesizing the degraded ends of the partially degraded double strand produced according to step (c), wherein steps (c) and (d) may be carried out sequentially or contemporaneously.
    • 一种用于生产具有改进性质的生物聚合物的方法,其中完成包括以下步骤的至少一个循环:(a)提供一群单链多核苷酸分子,其中单个多核苷酸分子包含同源和异源序列区段, 所述单链多核苷酸分子可以与所述群体内的所述单链多核苷酸分子中的其它单链多核苷酸分子形成双链多核苷酸分子;(b)从根据步骤(I)提供的单链多核苷酸分子群体形成双链多核苷酸分子 a)包含具有不同异源序列片段的双链多核苷酸分子;(c)部分和外核解离降解根据步骤(b)制备的双链多核苷酸分子的单链; 和(d)合成根据步骤(c)制备的部分降解的双链的降解末端的神经导向单支,其中步骤(c)和(d)可以顺序或同时进行。
    • 29. 发明授权
    • Method and device for multicolour 2-photon fluorescence coincidence analysis
    • 多色双光子荧光重合分析方法与装置
    • US07507582B2
    • 2009-03-24
    • US10333034
    • 2001-07-18
    • Katrin HeinzePetra SchwilleAndre KoltermannUlrich Kettling
    • Katrin HeinzePetra SchwilleAndre KoltermannUlrich Kettling
    • G01N21/64
    • G01N21/6408B82Y10/00B82Y20/00G01J2003/2866G01N2021/6441Y10T436/13
    • A method of fluorescence measurement on analytes, marked with different fluorescent markers, which have spectrally different fluorescence emissions, in a sample is described, having the following steps: illumination of the sample in the measurement volume using a laser to excite the fluorescence emission of the at least two fluorescent markers, the illumination of the measurement volume being performed with a maximum of one single laser line, having an excitation intensity sufficiently high that the fluorescent markers are jointly excited through 2-photon absorptions, detection of the fluorescence emission using at least two detector devices which are designed for light detection in different spectral ranges in accordance with the spectral fluorescence properties of the fluorescent markers, and performance of a cross-correlation and/or coincidence analysis of detector signals of the detector devices. A measurement device for performing the method is also described.
    • 描述了在样品中具有不同荧光标记的分析物上荧光测量的方法,所述分析物具有不同的荧光发射,具有以下步骤:使用激光照射测量体积中的样品以激发荧光发射 至少两个荧光标记物,用最大量的一条单一激光线照射测量体积,具有足够高的激发强度,使得荧光标记物通过双光子吸收共同激发,至少使用至少检测荧光发射 两个检测器装置被设计用于根据荧光标记的光谱荧光特性在不同光谱范围内进行光检测,以及执行检测器装置的检测器信号的互相关和/或重合分析。 还描述了用于执行该方法的测量装置。