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21. 发明申请

US20110262937A1 T1R HETERO-OLIGOMERIC TASTE RECEPTORS AND CELL LINES THAT EXPRESS SAID RECEPTORS AND USE THEREOF FOR IDENTIFICATION OF TASTE COMPOUNDS 有权
公开(公告)号：US20110262937A1
公开(公告)日：2011-10-27
申请号：US13099327
申请日：2011-05-02
申请人： Mark Zoller , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Elliot Adler , Hong Xu , Fernando Echeverri
发明人： Mark Zoller , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Elliot Adler , Hong Xu , Fernando Echeverri
IPC分类号： C07K14/705 , G01N33/554 , C12N5/071
CPC分类号： G01N33/5041 , A01K2217/05 , C07K14/705 , C07K2319/00 , C12Q1/6876 , C12Q2600/124 , C12Q2600/158 , G01N33/5008 , G01N33/566 , G01N2333/4719 , G01N2333/705 , G01N2333/726 , G01N2500/04
摘要： The present invention relates to the discovery that the T1R receptors assemble to form functional taste receptors. Particularly, it has been discovered that co-expression of T1R1 and T1R3 results in a taste receptor that responds to umami taste stimuli, including monosodium glutamate. Also, it has been discovered that co-expression of the T1R2 and T1R3 receptors results in a taste receptor that responds to sweet taste stimuli including naturally occurring and artificial sweeteners.Also the present invention relates to the use of hetero-oligomeric taste receptors comprising T1R1/T1R3 and T1R2/T1R3 in assays to identify compounds that respectively respond to umami taste stimuli and sweet taste stimuli.Further, the invention relates to the constitutive of cell lines that stably or transiently co-express a combination of T1R1 and T1R3; or T1R2 and T1R3; under constitutive or inducible conditions. The use of these cells lines in cell-based assays to identify umami and sweet taste modulatory compounds is also provided, particularly high throughput screening assays that detect receptor activity by use of fluorometric imaging.Finally, the invention relates to the discovery that some compounds, e.g., lactisole, inhibit both the activities of human T1R2/T1R3 and T1R1/T1R3 receptors, and accordingly the sweet and umami taste, suggesting that these receptors may be the only sweet and umami receptors.

22. 发明授权

US07906328B2 Cell lines that express hetero oligomeric taste receptors 有权
标题翻译：表达杂低聚味感受器的细胞系
公开(公告)号：US07906328B2
公开(公告)日：2011-03-15
申请号：US12270824
申请日：2008-11-13
申请人： Mark Zoller , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Elliot Adler , Hong Xu , Fernando Echeverri
发明人： Mark Zoller , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Elliot Adler , Hong Xu , Fernando Echeverri
IPC分类号： C12N5/00 , C12N5/02 , C12N1/20 , C12N15/00 , C12N15/74 , C12P21/06 , C07K1/00 , C07K14/00 , C07K17/00
CPC分类号： G01N33/5041 , A01K2217/05 , C07K14/705 , C07K2319/00 , C12Q1/6876 , C12Q2600/124 , C12Q2600/158 , G01N33/5008 , G01N33/566 , G01N2333/4719 , G01N2333/705 , G01N2333/726 , G01N2500/04
摘要： The present invention relates to the discovery that the T1R receptors assemble to form functional taste receptors. Particularly, it has been discovered that co-expression of T1R1 and T1R3 results in a taste receptor that responds to umami taste stimuli, including monosodium glutamate. Also, it has been discovered that co-expression of the T1R2 and T1R3 receptors results in a taste receptor that responds to sweet taste stimuli including naturally occurring and artificial sweeteners. Also the present invention relates to the use of hetero-oligomeric taste receptors comprising T1R1/T1R3 and T1R2/T1R3 in assays to identify compounds that respectively respond to umami taste stimuli and sweet taste stimuli. Further, the invention relates to the constitutive of cell lines that stably or transiently co-express a combination of T1R1 and T1R3; or T1R2 and T1R3; under constitutive or inducible conditions. The use of these cells lines in cell-based assays to identify umami and sweet taste modulatory compounds is also provided, particularly high throughput screening assays that detect receptor activity by use of fluorometric imaging.
摘要翻译：本发明涉及T1R受体组装形成功能性味觉受体的发现。特别地，已经发现，T1R1和T1R3的共表达导致对鲜味刺激（包括谷氨酸钠）起反应的味觉感受器。此外，已经发现，T1R2和T1R3受体的共表达导致对包括天然存在的和人造甜味剂的甜味刺激有反应的味觉受体。本发明还涉及在测定中使用包含T1R1 / T1R3和T1R2 / T1R3的异低聚味觉感受器来鉴定分别响应于鲜味刺激和甜味刺激的化合物。此外，本发明涉及稳定或瞬时共表达T1R1和T1R3的组合的细胞系的组成; 或T1R2和T1R3; 在组成型或诱导性条件下。还提供了在基于细胞的测定中使用这些细胞系以鉴定鲜味和甜味调节化合物，特别是通过使用荧光成像检测受体活性的高通量筛选测定。

23. 发明授权

US07763431B1 Binding assays that use the T1R2/T1R3 (sweet) taste receptor to identify compounds that elicit or modulate sweet taste 有权
标题翻译：使用T1R2 / T1R3（甜味）味觉受体的结合测定法鉴定引起或调节甜味的化合物
公开(公告)号：US07763431B1
公开(公告)日：2010-07-27
申请号：US10725076
申请日：2003-12-02
申请人： Mark Zoller , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Elliot Adler , Hong Xu , Fernando Echeverri
发明人： Mark Zoller , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Elliot Adler , Hong Xu , Fernando Echeverri
IPC分类号： G01N33/53 , G01N33/567 , C12P21/06 , C12N1/20 , C12N15/74 , C12N15/00 , C12N15/02 , C07K1/00 , C07K14/00 , C07K17/00 , C07H21/04
CPC分类号： C07K14/705 , A01K2217/05 , G01N2333/726
摘要： Binding assays for identifying compounds that induce or modulate the T1R2/T1R3 (sweet) receptor associated taste are provided. These binding assays detect the specific binding or a component to a T1R2/T1R3 (sweet) taste receptor or detect modulation (inhibition or enhancement) of the binding of another compound, e.g., saccharin or another sweetener to a T1R2/T1R3 (sweet) taste receptor. Compounds that are identified in these binding assays have potential application of T1R2/T1R3 sweet taste receptor modulators and can be used as flavor additives in compositions for human or animal consumption.
摘要翻译：提供了用于鉴定诱导或调节T1R2 / T1R3（甜）受体相关味道的化合物的结合测定法。这些结合测定法检测T1R2 / T1R3（甜味）味觉受体的特异性结合或成分，或检测另一种化合物（例如糖精或另一种甜味剂）与T1R2 / T1R3（甜味）味道的结合的调节（抑制或增强）受体。在这些结合测定中鉴定的化合物具有T1R2 / T1R3甜味受体调节剂的潜在应用，并且可以用作用于人或动物消费的组合物中的风味添加剂。

24. 发明申请

US20090221067A1 Cell lines that express hetero oligomeric taste receptors 有权
标题翻译：表达杂低聚味感受器的细胞系
公开(公告)号：US20090221067A1
公开(公告)日：2009-09-03
申请号：US12270824
申请日：2008-11-13
申请人： Mark Zoller , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Elliot Adler , Hong Xu , Fernando Echeverri
发明人： Mark Zoller , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Elliot Adler , Hong Xu , Fernando Echeverri
IPC分类号： C12N5/10 , C12N1/21 , C12N1/19
CPC分类号： G01N33/5041 , A01K2217/05 , C07K14/705 , C07K2319/00 , C12Q1/6876 , C12Q2600/124 , C12Q2600/158 , G01N33/5008 , G01N33/566 , G01N2333/4719 , G01N2333/705 , G01N2333/726 , G01N2500/04
摘要： The present invention relates to the discovery that the T1R receptors assemble to form functional taste receptors. Particularly, it has been discovered that co-expression of T1R1 and T1R3 results in a taste receptor that responds to umami taste stimuli, including monosodium glutamate. Also, it has been discovered that co-expression of the T1R2 and T1R3 receptors results in a taste receptor that responds to sweet taste stimuli including naturally occurring and artificial sweeteners. Also the present invention relates to the use of hetero-oligomeric taste receptors comprising T1R1/T1R3 and T1R2/T1R3 in assays to identify compounds that respectively respond to umami taste stimuli and sweet taste stimuli. Further, the invention relates to the constitutive of cell lines that stably or transiently co-express a combination of T1R1 and T1R3; or T1R2 and T1R3; under constitutive or inducible conditions. The use of these cells lines in cell-based assays to identify umami and sweet taste modulatory compounds is also provided, particularly high throughput screening assays that detect receptor activity by use of fluorometric imaging.
摘要翻译：本发明涉及T1R受体组装形成功能性味觉受体的发现。特别地，已经发现，T1R1和T1R3的共表达导致对鲜味刺激（包括谷氨酸钠）起反应的味觉感受器。此外，已经发现，T1R2和T1R3受体的共表达导致对包括天然存在的和人造甜味剂的甜味刺激有反应的味觉受体。本发明还涉及在测定中使用包含T1R1 / T1R3和T1R2 / T1R3的异低聚味觉感受器来鉴定分别响应于鲜味刺激和甜味刺激的化合物。此外，本发明涉及稳定或瞬时共表达T1R1和T1R3的组合的细胞系的组成; 或T1R2和T1R3; 在组成型或诱导性条件下。还提供了在基于细胞的测定中使用这些细胞系以鉴定鲜味和甜味调节化合物，特别是通过使用荧光成像检测受体活性的高通量筛选测定。

25. 发明申请

US20090215174A1 Methods of inhibiting umami taste receptors 有权
标题翻译：抑制鲜味受体的方法
公开(公告)号：US20090215174A1
公开(公告)日：2009-08-27
申请号：US12270848
申请日：2008-11-13
申请人： Mark Zoller , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Elliot Adler , Hong Xu , Fernando Echeverri
发明人： Mark Zoller , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Elliot Adler , Hong Xu , Fernando Echeverri
IPC分类号： C12N5/06
CPC分类号： G01N33/5041 , A01K2217/05 , C07K14/705 , C07K2319/00 , C12Q1/6876 , C12Q2600/124 , C12Q2600/158 , G01N33/5008 , G01N33/566 , G01N2333/4719 , G01N2333/705 , G01N2333/726 , G01N2500/04
摘要： Methods of inhibiting umami taste receptors are provided. These methods comprise contacting T1R1/T1R3 umami taste receptors with a sweet-taste inhibitor that also inhibits both the T1R1/T1R3 umami taste receptor and the T1R2/T1R3 sweet taste receptor.
摘要翻译：提供了抑制鲜味受体的方法。这些方法包括使T1R1 / T1R3鲜味受体与也抑制T1R1 / T1R3鲜味受体和T1R2 / T1R3甜味受体的甜味抑制剂接触。

26. 发明授权

US07301009B2 Isolated (T1R1/T1R3) umami taste receptors that respond to umami taste stimuli 有权
标题翻译：隔离（T1R1 / T1R3）鲜味感受器响应鲜味刺激
公开(公告)号：US07301009B2
公开(公告)日：2007-11-27
申请号：US10725080
申请日：2003-12-02
申请人： Mark Zoller , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Elliot Adler , Hong Xu , Fernando Echeverri
发明人： Mark Zoller , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Elliot Adler , Hong Xu , Fernando Echeverri
IPC分类号： C07K1/00 , C07K14/00 , C07K17/00
CPC分类号： G01N33/5008 , A01K2217/05 , C07K14/705 , G01N33/5041 , G01N33/566 , G01N2333/726
摘要： Isolated or biologically pure taste receptors comprised of at least one T1R1 and T1R3 polypeptide and compositions containing are provided. These taste receptors specifically bind to and/or are specifically activated by umami taste stimuli such as glutamate compounds. These taste receptors are useful in screening assays for identifying compounds that elicit or modulate (enhance or inhibit) umami taste. Such compounds can be used as additives and flavorants to improve the taste of compositions for human or animal consumption. In preferred embodiments, the T1R1/T1R3 receptor will be comprised of human T1R1/human T1R3 expressed in association with a suitable G protein, e.g. Gα15.
摘要翻译：提供了由至少一种T1R1和T1R3多肽组成的分离的或生物学上纯的味觉受体和包含的组合物。这些味觉受体特异性结合于和/或由鲜味刺激物如谷氨酸酯化合物特异性活化。这些味觉受体可用于鉴定引发或调节（增强或抑制）鲜味的化合物的筛选测定。这些化合物可用作添加剂和调味剂，以改善用于人或动物消费的组合物的味道。在优选的实施方案中，T1R1 / T1R3受体将由与适合的G蛋白（例如， α15α。

27. 发明授权

US07297772B2 Isolated (T1R2/T1R3) sweet taste receptors that respond to sweet taste stimuli 有权
标题翻译：分离（T1R2 / T1R3）甜味受体，对甜味刺激有反应
公开(公告)号：US07297772B2
公开(公告)日：2007-11-20
申请号：US10725037
申请日：2003-12-02
申请人： Mark Zoller , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Elliot Adler , Hong Xu , Fernando Echeverri
发明人： Mark Zoller , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Elliot Adler , Hong Xu , Fernando Echeverri
IPC分类号： C07K1/00 , C07K14/00 , C07K17/00
CPC分类号： G01N33/5041 , A01K2217/05 , C07K14/705 , C07K2319/00 , C12Q1/6876 , C12Q2600/124 , C12Q2600/158 , G01N33/5008 , G01N33/566 , G01N2333/4719 , G01N2333/705 , G01N2333/726 , G01N2500/04
摘要： Isolated or biologically pure taste receptors comprised of at least one T1R2 and T1R3 polypeptide and compositions containing are provided. These taste receptors specifically bind and/or are specifically activated by sweet taste stimuli such as natural and artificial sweetener compounds. These taste receptors are useful in screening assays for identifying compounds that elicit or modulate (enhance or inhibit) sweet taste. These compounds can be used as additives and flavorants to improve the taste of compositions for human or animal consumption. In preferred embodiments, the T1R2/T1R3 receptor will be comprised of human T1R2/human T1R3 expressed in association with a suitable G protein, preferably Gaa15.
摘要翻译：提供了由至少一种T 1 R 2和T 1 R 3多肽组成的分离的或生物学上纯的味觉受体，以及含有的组合物。这些味觉受体特异性结合和/或由甜味刺激物例如天然和人造甜味剂化合物特异性活化。这些味觉受体可用于鉴定诱导或调节（增强或抑制）甜味的化合物的筛选测定。这些化合物可用作添加剂和调味剂，以改善用于人或动物消费的组合物的味道。在优选的实施方案中，T 1 R 2 / T 1 R 3受体将由与合适的G蛋白（优选Gaαa 15）结合表示的人T 1 R 2 /人T 1 R 3组成。

28. 发明授权

US08206938B2 In vitro methods of detecting cells sensitive to sweet tastants by detecting expression of hetero-oligomeric T1R2/T1R3 taste receptors 有权
标题翻译：通过检测异低聚T1R2 / T1R3味觉受体的表达来检测对甜味滋味物敏感的细胞的体外方法
公开(公告)号：US08206938B2
公开(公告)日：2012-06-26
申请号：US13099327
申请日：2011-05-02
申请人： Mark Zoller , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Elliot Adler , Hong Xu , Fernando Echeverri
发明人： Mark Zoller , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Elliot Adler , Hong Xu , Fernando Echeverri
IPC分类号： G01N33/53 , G01N33/567 , C12Q1/68
CPC分类号： G01N33/5041 , A01K2217/05 , C07K14/705 , C07K2319/00 , C12Q1/6876 , C12Q2600/124 , C12Q2600/158 , G01N33/5008 , G01N33/566 , G01N2333/4719 , G01N2333/705 , G01N2333/726 , G01N2500/04
摘要： The present invention relates to the discovery that the T1R receptors assemble to form functional taste receptors. Particularly, it has been discovered that co-expression of T1R1 and T1R3 results in a taste receptor that responds to umami taste stimuli, including monosodium glutamate. Also, it has been discovered that co-expression of the T1R2 and T1R3 receptors results in a taste receptor that responds to sweet taste stimuli including naturally occurring and artificial sweeteners.Also the present invention relates to the use of hetero-oligomeric taste receptors comprising T1R1/T1R3 and T1R2/T1R3 in assays to identify compounds that respectively respond to umami taste stimuli and sweet taste stimuli.Further, the invention relates to the constitutive of cell lines that stably or transiently co-express a combination of T1R1 and T1R3; or T1R2 and T1R3; under constitutive or inducible conditions. The use of these cells lines in cell-based assays to identify umami and sweet taste modulatory compounds is also provided, particularly high throughput screening assays that detect receptor activity by use of fluorometric imaging.Finally, the invention relates to the discovery that some compounds, e.g., lactisole, inhibit both the activities of human T1R2/T1R3 and T1R1/T1R3 receptors, and accordingly the sweet and umami taste, suggesting that these receptors may be the only sweet and umami receptors.
摘要翻译：本发明涉及T1R受体组装形成功能性味觉受体的发现。特别地，已经发现，T1R1和T1R3的共表达导致对鲜味刺激（包括谷氨酸钠）起反应的味觉感受器。此外，已经发现，T1R2和T1R3受体的共表达导致对包括天然存在的和人造甜味剂的甜味刺激有反应的味觉受体。本发明还涉及在测定中使用包含T1R1 / T1R3和T1R2 / T1R3的异低聚味觉感受器来鉴定分别响应于鲜味刺激和甜味刺激的化合物。此外，本发明涉及稳定或瞬时共表达T1R1和T1R3的组合的细胞系的组成; 或T1R2和T1R3; 在组成型或诱导性条件下。还提供了在基于细胞的测定中使用这些细胞系以鉴定鲜味和甜味调节化合物，特别是通过使用荧光成像检测受体活性的高通量筛选测定。最后，本发明涉及一些发现，一些化合物，例如乳酸，抑制人T1R2 / T1R3和T1R1 / T1R3受体的活性，因此抑制甜味和鲜味，这表明这些受体可能是唯一的甜味和鲜味受体。

29. 发明授权

US08067185B2 Methods of quantifying taste of compounds for food or beverages 有权
标题翻译：量化化合物食物或饮料味道的方法
公开(公告)号：US08067185B2
公开(公告)日：2011-11-29
申请号：US12270841
申请日：2008-11-13
申请人： Mark Zoller , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Elliot Adler , Hong Xu , Fernando Echeverri
发明人： Mark Zoller , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Elliot Adler , Hong Xu , Fernando Echeverri
IPC分类号： G01N33/53 , G01N33/567 , G01N33/566
CPC分类号： G01N33/5041 , A01K2217/05 , C07K14/705 , C07K2319/00 , C12Q1/6876 , C12Q2600/124 , C12Q2600/158 , G01N33/5008 , G01N33/566 , G01N2333/4719 , G01N2333/705 , G01N2333/726 , G01N2500/04
摘要： Methods of quantifying the taste of compounds for food and beverages are provided. These methods comprise contacting the compounds with an isolated heteromeric receptor comprising at least one T1R1 polypeptide and at least one T1R3 polypeptide.
摘要翻译：提供了定量食品和饮料化合物味道的方法。这些方法包括使化合物与包含至少一种T1R1多肽和至少一种T1R3多肽的分离的异聚受体接触。

30. 发明授权

US08039590B2 Heteromeric taste receptors comprising T1R2/T1R3 domains 有权
标题翻译：含有T1R2 / T1R3结构域的异构味觉受体
公开(公告)号：US08039590B2
公开(公告)日：2011-10-18
申请号：US12270857
申请日：2008-11-14
申请人： Mark Zoller , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Elliot Adler , Hong Xu , Fernando Echeverri
发明人： Mark Zoller , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Elliot Adler , Hong Xu , Fernando Echeverri
IPC分类号： C07K1/00 , C07K14/00 , C07K17/00
CPC分类号： G01N33/5008 , A01K2217/05 , C07K14/705 , G01N33/5041 , G01N33/566 , G01N2333/726
摘要： Heteromeric taste receptors are provided. These receptors comprise a first polypeptide which comprises extracellular and transmembrane domains wherein the transmembrane domains are at least 95% identical to the transmembrane domains of specific T1R2 polypeptides, and the extracellular domains are at least 95% identical to the corresponding extracellular domains of the specific T1R2 polypeptide or a different GPCR; and a second polypeptide which comprises extracellular and transmembrane domains wherein the transmembrane domains are at least 95% identical to the transmembrane domains of specific T1R3 polypeptides, and the extracellular domains are at least 95% identical to the corresponding extracellular domains of the specific T1R3 polypeptide or that of a different GPCR.
摘要翻译：提供异味味觉受体。这些受体包含第一多肽，其包含细胞外和跨膜结构域，其中跨膜结构域与特异性T1R2多肽的跨膜结构域至少95％相同，并且细胞外结构域与特异性T1R2的相应胞外结构域至少95％相同多肽或不同的GPCR; 和包含细胞外和跨膜结构域的第二多肽，其中所述跨膜结构域与特异性T1R3多肽的跨膜结构域至少95％相同，并且细胞外结构域与特异性T1R3多肽的相应细胞外结构域至少95％相同，或不同的GPCR。

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