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    • 24. 发明公开
    • SOYBEAN TRANSFORMATION METHOD
    • SOJABOHNENTRANSFORMATIONSVERFAHREN
    • EP3051940A4
    • 2017-03-29
    • EP14850319
    • 2014-10-02
    • DOW AGROSCIENCES LLC
    • CHENNAREDDY SIVARAMA REDDYPAREDDY DAYAKARSAMUEL JAYAKUMAR PONSARRIA-MILAN RODRIGOCICAK TOBY
    • A01H5/00C12N15/82C12Q1/48
    • G01N33/68C12N15/8201
    • The present disclosure relates in part to a method for identifying a soybean germline transformant from a population of soybean transformants by incorporating a selection agent within rooting medium used in tissue culture during the soybean transformation process. The soybean germline transformants are selected from a population of soybean transformants which are comprised of a combination of non-germline and germline soybean transformants. The soybean non-germline transformants are identified and eliminated early in the transformation process. The soybean germline transformants are identified and selected for culturing into mature soybean plants. The method is readily applicable for screening and obtaining a soybean germline transformant at an early stage in the soybean transformation process.
    • 本公开内容部分地涉及通过在大豆转化过程中在组织培养中使用的生根培养基中掺入选择剂来从大豆转化体群体中鉴定大豆种系转化体的方法。 大豆种系转化体选自由非种系和种系大豆转化体的组合组成的大豆转化体群体。 在转化过程中早期鉴定和消除大豆非种系转化体。 鉴定和选择大豆种系转化体用于培养成熟大豆植物。 该方法适用于大豆转化过程早期筛选和获得大豆种系转化体。