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21. 发明申请

WO2013085361A3 MUTANT MICROORGANISM HAVING A HIGH 4-HYDROXYBUTYRIC ACID PRODUCTION CAPACITY, AND METHOD FOR PREPARING 4-HYDROXYBUTYRIC ACID USING SAME 审中-公开
标题翻译：具有高4-羟基丁酸生产能力的突变微生物以及使用其制备4-羟基丁酸的方法
公开(公告)号：WO2013085361A3
公开(公告)日：2013-10-17
申请号：PCT/KR2012010665
申请日：2012-12-07
申请人： KOREA ADVANCED INST SCI & TECH
发明人： LEE SANG YUP , CHOI SOL , LEE JEONG WOOK , PARK SI JAE
IPC分类号： C12N1/00
CPC分类号： C12N9/0006 , C12N9/001 , C12N9/1025 , C12N9/93 , C12P7/42 , C12P7/52
摘要： The present invention relates to a mutant microorganism having a high 4-hydroxybutyric acid production capacity and to a method for preparing 4-hydroxybutyric acid using the mutant microorganism. More particularly, the present invention relates to a mutant microorganism having a high 4-hydroxybutyric acid production capacity, in which genes for synthesizing 4-hydroxybutyric acid from succinic acid are amplified, genes in an anaplerotic pathway are amplified, and competitive genes are deleted so as to increase the 4-hydroxybutyric acid production capacity. The present invention also relates to a method for preparing 4-hydroxybutyric acid with a high yield rate by culturing the mutant microorganism under microaerobic conditions. The present invention enables the preparation of a mutant microorganism having a high 4-hydroxybutyric acid production capacity and the use of the mutant microorganism, and therefore is useful in the preparation of 4-hydroxybutyric acid with a high yield rate.
摘要翻译：本发明涉及具有高4-羟基丁酸生产能力的突变微生物和使用突变微生物制备4-羟基丁酸的方法。更具体地说，本发明涉及一种具有高4-羟基丁酸生产能力的突变微生物，其中扩增来自琥珀酸的4-羟基丁酸合成基因被扩增，并且在绝经途径中的基因被扩增，并且竞争性基因被删除以提高4-羟基丁酸的生产能力。本发明还涉及通过在微需氧条件下培养突变型微生物来制备高产率的4-羟基丁酸的方法。本发明能够制备具有高4-羟基丁酸生产能力的突变微生物和突变微生物的用途，因此可用于以高产率制备4-羟基丁酸。

22. 发明公开

EP1664094A4 COMPOSITION FOR PROTECTING PROTEINS DEGRADATION COMPRISING SMALL HEAT SHOCK PROTEINS (sHSPs) AND METHOD OF TWO-DIMENSIONAL GEL ELECTROPHORESIS USING THE sHSPs 有权
标题翻译： KLEINE HITZESCHOCK蛋白质（SHSPS）ENTHALTENDE ZUSAMMENSETZUNGEN ZUM SCHUTZ VOR PROTEINABBAU UND VERFAHREN ZUR ZWEIDIMENSIONALEN GELELEKTROPHORESE UNTER ANWENDUNG DER SHSPS
公开(公告)号：EP1664094A4
公开(公告)日：2006-09-27
申请号：EP03774298
申请日：2003-11-24
申请人： KOREA ADVANCED INST SCI & TECH
发明人： LEE SANG YUP , HAN MEE JUNG , PARK SI JAE
IPC分类号： C07K14/00 , G01N33/483 , C07K14/195 , C07K14/245 , C12N15/09 , G01N27/447 , G01N33/68
CPC分类号： C07K14/195 , C07K14/00
摘要： The present invention relates to a composition containing sHSPs for prevention of protein degradation and a composition for two-dimensional gel electrophoresis. Furthermore, the present invention relates to the improved method of two-dimensional gel electrophoresis, which is characterized by using sHSPs. According to the present invention, decreasing of protein spots was prevented in the two-dimensional gel electrophoresis, thereby obtaining two-dimensional gel with much more protein spots.
摘要翻译：本发明涉及含有用于防止蛋白质降解的sHSPs和用于双向凝胶电泳的组合物的组合物。此外，本发明涉及二维凝胶电泳的改进方法，其特征在于使用sHSP。根据本发明，在二维凝胶电泳中阻止了蛋白质斑点的减少，由此获得蛋白质斑点更多的二维凝胶。

23. 发明公开

EP2684949A4 RECOMBINANT MICROORGANISM HAVING ABILITY TO PRODUCE [LACTATE-CO-GLYCOLATE]COPOLYMER FROM GLUCOSE, AND METHOD FOR PREPARING [LACTATE-CO-GLYCOLATE]COPOLYMER USING SAME 有权
标题翻译：需要有能力制备[LACTAT-CO-乙醇酸]葡萄糖共聚物，并产生[LACTAT-CO-乙醇酸]的方法的重组微生物共聚物SO
公开(公告)号：EP2684949A4
公开(公告)日：2014-10-15
申请号：EP12757647
申请日：2012-03-09
申请人： KOREA ADVANCED INST SCI & TECH , KOREA RES INST CHEM TECH
发明人： LEE SANG YUP , PARK SI JAE , LEE SEUNG HWAN , SONG BONG KEUN , JUNG YU KYUNG , LEE TAE WOO
IPC分类号： C12N1/21 , C12N15/31 , C12N15/52 , C12P7/40
CPC分类号： C12P7/625 , C12N9/0006 , C12N9/1029 , C12N15/70
摘要： There is provided a recombinant microorganism having producibility of poly(lactate-co-glycolate) from glucose, and more particularly, a recombinant microorganism having producibility of poly(lactate-co-glycolate) without adding an exogenous glycolate precursor, and a method of preparing [poly(preparing lactate-co-glycolate)] using the same. According to the present invention, the poly(lactate-co-glycolate) in which the concentration of the glycolate fraction is high may be prepared at a high concentration without supplying exogenous glyoxylate. Therefore, the present invention may be effectively used for treatment.

24. 发明公开

EP1604015A4 METHOD FOR PRODUCING TARGET PROTEINS BY DELETING OR AMPLIFYING ibpA AND/OR ibpB GENE CODING FOR INCLUSION BODY-ASSOCIATED PROTEINS 有权
标题翻译：方法的靶蛋白借助于距离或者是身体相关蛋白编码IBPA AND / OR ibpB基因扩增夹杂物
公开(公告)号：EP1604015A4
公开(公告)日：2006-09-20
申请号：EP03816262
申请日：2003-07-10
申请人： KOREA ADVANCED INST SCI & TECH
发明人： LEE SANG YUP , HAN MEE JUNG , PARK SI JAE
IPC分类号： C07K14/245 , C07K14/54 , C07K14/57 , C07K14/65 , C12N1/21 , C12N15/70 , C12P21/02
CPC分类号： C12P21/02 , C07K14/245 , C07K14/5434 , C07K14/57 , C07K14/65 , C12N15/70

25. 发明专利

AU2003284750A1 COMPOSITION FOR PROTECTING PROTEINS DEGRADATION COMPRISING SMALL HEAT SHOCK PROTEINS (sHSPs) AND METHOD OF TWO-DIMENSIONAL GEL ELECTROPHORESIS USING THE sHSPs 未知
公开(公告)号：AU2003284750A1
公开(公告)日：2005-03-29
申请号：AU2003284750
申请日：2003-11-24
申请人： KOREA ADVANCED INST SCI & TECH
发明人： LEE SANG YUP , HAN MEE JUNG , PARK SI JAE
IPC分类号： G01N33/483 , C07K14/00 , C07K14/195 , C07K14/245 , C12N15/09 , G01N27/447 , G01N33/68
摘要： The present invention relates to a composition containing sHSPs for prevention of protein degradation and a composition for two-dimensional (2-D) gel electrophoresis. Furthermore, the present invention relates to the improved method of 2-D gel electrophoresis, which is characterized by using sHSPs. According to the present invention, decreasing of protein spots was prevented in the 2-D gel electrophoresis, thereby obtaining 2-D gel with much more protein spots.

26. 发明专利

AU2003247177A1 METHOD FOR PRODUCING TARGET PROTEINS BY DELETING OR AMPLIFYINGibpA AND/OR ibpB GENE CODING FOR INCLUSION BODY-ASSOCIATED PROTEINS 未知
公开(公告)号：AU2003247177A1
公开(公告)日：2004-09-30
申请号：AU2003247177
申请日：2003-07-10
申请人： KOREA ADVANCED INST SCI & TECH
发明人： LEE SANG YUP , HAN MEE JUNG , PARK SI JAE
IPC分类号： C07K14/245 , C07K14/54 , C07K14/57 , C07K14/65 , C12N1/21 , C12N15/70 , C12P21/02
摘要： A method for producing target proteins by deleting or amplifying ibpA and/or ibpB genes coding for inclusion body-associated proteins. Two methods for producing target proteins using ibpA and/or ibpB genes coding for inclusion body-associated proteins of E. coli are described. The first method enhances the secretory production and activity of target proteins using ibpA and/or ibpB genes-deleted bacteria. The second method enhances the production of target proteins in the cytoplasm and also converts the target proteins from soluble form to insoluble inclusion body, using ibpA and/or ibpB gene-amplified bacteria.

27. 发明专利

GB2425312B Method for cell surface display of target proteins using fadl of e coli 未知
公开(公告)号：GB2425312B
公开(公告)日：2008-12-17
申请号：GB0615560
申请日：2004-08-30
申请人： KOREA ADVANCED INST SCI & TECH
发明人： LEE SANG YUP , LEE SEUNG HWAN , PARK SI JAE
IPC分类号： C12N15/70 , C07K14/245 , C07K19/00 , C12N15/63 , C12N15/64 , C12N15/66
摘要： The present invention relates to an expression vector which can effectively express target proteins or peptides on the surface of cells using an outer membrane protein (FadL) of E. coli as a surface anchoring motif. Also, the present invention relates to microorganisms transformed with the expression vector, and a method for stably expressing large amounts of target proteins on the surface of cells by culturing the transformed microorganisms. Furthermore, the present invention relates to a production method of protein arrays, a production method of antibodies, and a bioconversion method, the methods being characterized by using target proteins which have been expressed on the cell surface by the inventive method. In addition, the present invention relates to a method for improving target proteins by the inventive surface expression method. The present invention allows target proteins with normal functions to be expressed on an outer cell membrane. Thus, the present invention will be useful in recombinant live vaccines, the screening of various peptides or antibodies, whole-cell adsorbents for heavy metal removal or waste water treatment, whole-cell bioconversion, and the like.

28. 发明专利

CA2537880A1 COMPOSITION FOR PROTECTING PROTEINS DEGRADATION COMPRISING SMALL HEAT SHOCK PROTEINS (SHSPS) AND METHOD OF TWO-DIMENSIONAL GEL ELECTROPHORESIS USING THE SHSPS 未知
公开(公告)号：CA2537880A1
公开(公告)日：2005-03-17
申请号：CA2537880
申请日：2003-11-24
申请人： KOREA ADVANCED INST SCI & TECH
发明人： PARK SI JAE , HAN MEE JUNG , LEE SANG YUP
IPC分类号： C07K14/00 , G01N33/483 , C07K14/195 , C07K14/245 , C12N15/09 , G01N27/447 , G01N33/68
摘要： The present invention relates to a composition containing sHSPs for preventi on of protein degradation and a composition for two-dimensional gel electrophoresis. Furthermore, the present invention relates to the improved method of two-dimensional gel electrophoresis, which is characterized by usi ng sHSPs. According to the present invention, decreasing of protein spots was prevented in the two-dimensional gel electrophoresis, thereby obtaining two- dimensional gel with much more protein spots.

29. 发明专利

CA2537880C COMPOSITION FOR PROTECTING PROTEINS DEGRADATION COMPRISING SMALL HEAT SHOCK PROTEINS (SHSPS) AND METHOD OF TWO-DIMENSIONAL GEL ELECTROPHORESIS USING THE SHSPS 未知
公开(公告)号：CA2537880C
公开(公告)日：2011-11-01
申请号：CA2537880
申请日：2003-11-24
申请人： KOREA ADVANCED INST SCI & TECH
发明人： LEE SANG YUP , HAN MEE JUNG , PARK SI JAE
IPC分类号： C07K14/00 , G01N33/483 , C07K14/195 , C07K14/245 , C12N15/09 , G01N27/447 , G01N33/68
摘要： The present invention relates to a composition containing sHSPs for prevention of protein degradation and a composition for two-dimensional gel electrophoresis. Furthermore, the present invention relates to the improved method of two-dimensional gel electrophoresis, which is characterized by using sHSPs. According to the present invention, decreasing of protein spots was prevented in the two-dimensional gel electrophoresis, thereby obtaining two-dimensional gel with much more protein spots.

30. 发明专利

DE60324712D1 未知
公开(公告)号：DE60324712D1
公开(公告)日：2008-12-24
申请号：DE60324712
申请日：2003-11-24
申请人： KOREA ADVANCED INST SCI & TECH
发明人： LEE SANG YUP , HAN MEE JUNG , PARK SI JAE
IPC分类号： C07K14/00 , G01N33/483 , C07K14/195 , C07K14/245 , C12N15/09 , G01N27/447 , G01N33/68
摘要： The present invention relates to a composition containing sHSPs for prevention of protein degradation and a composition for two-dimensional (2-D) gel electrophoresis. Furthermore, the present invention relates to the improved method of 2-D gel electrophoresis, which is characterized by using sHSPs. According to the present invention, decreasing of protein spots was prevented in the 2-D gel electrophoresis, thereby obtaining 2-D gel with much more protein spots.

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