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21. 发明授权

US06936444B1 Process for the preparation of L-phosphinothricine by enzymatic transamination with aspartate 有权
标题翻译：用天冬氨酸酶转氨酶制备L-膦丝菌素的方法
公开(公告)号：US06936444B1
公开(公告)日：2005-08-30
申请号：US10018331
申请日：2000-06-30
申请人： Klaus Bartsch
发明人： Klaus Bartsch
IPC分类号： C12P7/46 , C07F9/38 , C12N1/20 , C12P1/04 , C12P13/04 , C12R1/00
CPC分类号： C12P13/04
摘要： The patent application describes a process for the enzymatic chiral synthesis of L-phosphinothricin by transamination from its corresponding keto acid PPO with aspartate as amino donor. It is possible by a suitable reaction procedure to achieve a quantitative conversion on use of approximately equimolar amounts of amino donor and acceptor with complete consumption of the donor amino acid aspartate. The use of thermally stable transaminases makes a higher reaction rate and correspondingly large space/time yields possible.
摘要翻译：该专利申请描述了通过其天冬氨酸作为氨基供体的相应酮酸PPO的转氨酶的酶促手性合成L-膦丝菌素的方法。通过合适的反应程序可以实现使用大约等摩尔量的氨基供体和受体的完全消耗供体氨基酸天冬氨酸的定量转化。使用热稳定的转氨酶使反应速度更高，相应地大的空间/时间产生成为可能。

22. 发明授权

US6051408A Gene and gene structure coding for an aminotransferase, and microorganisms which express this gene 失效
标题翻译：编码氨基转移酶的基因和基因结构，以及表达该基因的微生物
公开(公告)号：US6051408A
公开(公告)日：2000-04-18
申请号：US978817
申请日：1997-11-26
申请人： Klaus Bartsch , Arno Schulz , Eugen Uhlmann
发明人： Klaus Bartsch , Arno Schulz , Eugen Uhlmann
IPC分类号： C12N1/21 , C12N9/10 , C12N15/00 , C12N15/09 , C12N15/54 , C12P13/04 , C12R1/19 , C12P13/00
CPC分类号： C12P13/04 , C12N9/1096 , Y10S435/849
摘要： The preparation of L-2-amino-4-methylphosphinobutyric acid (L-PPT) by transamination of (3-carboxy-3-oxopropyl)-methylphosphinic acid with the aid of the L-PPT-specific transaminase from E. coli DH 1 is very much more efficient when the gene coding for this enzyme is isolated, incorporated into a plasmid and then a microorganism is transformed therewith.
摘要翻译：借助于大肠杆菌DH1的L-PPT特异性转氨酶，通过（3-羧基-3-氧代丙基） - 甲基次膦酸的氨基转移制备L-2-氨基-4-甲基膦酰基丁酸（L-PPT）当编码该酶的基因被分离时，并入质粒中，然后用微生物进行转化，则效率更高。

23. 发明授权

US5162212A Transaminase from E. coli DH-1 (ATCC 33849) 失效
标题翻译：来自大肠杆菌DH-1（ATCC 33849）的转氨酶
公开(公告)号：US5162212A
公开(公告)日：1992-11-10
申请号：US854516
申请日：1992-03-20
申请人： Arno Schulz , Klaus Bartsch , Dominique Tripier , Klaus Sauber
发明人： Arno Schulz , Klaus Bartsch , Dominique Tripier , Klaus Sauber
IPC分类号： C12N9/10 , C12N11/08 , C12P13/04 , C12R1/19
CPC分类号： C12N11/08 , C12N9/1096 , C12P13/04 , Y10S435/849
摘要： A new transaminase has been isolated from E. coli DH-1 (ATCC 33849). It is possible to use the transaminase with great efficiency to prepare L-phosphinothricin and gamma-aminobutyric acid from appropriate precursors by transferring an amino group from glutamate.
摘要翻译：已经从大肠杆菌DH-1（ATCC 33849）中分离出新的转氨酶。通过从谷氨酸转移氨基，可以高效地使用转氨酶从合适的前体制备L-膦丝菌素和γ-氨基丁酸。

24. 发明授权

US5077399A Phosphinothricin-resistance gene 失效
标题翻译：磷酸耐药基因
公开(公告)号：US5077399A
公开(公告)日：1991-12-31
申请号：US515671
申请日：1990-04-27
申请人： Dieter Brauer , Klaus Bartsch , Gunter Donn
发明人： Dieter Brauer , Klaus Bartsch , Gunter Donn
IPC分类号： A01H5/00 , A01N63/00 , C02F3/34 , C12N9/10 , C12N15/00 , C12N15/09 , C12N15/82 , C12R1/01 , C12R1/05 , C12R1/38 , C12R1/425
CPC分类号： C12N9/1029 , C12N15/8277 , Y10S435/829
摘要： Phosphinothricin (PTC) selection of bacteria which are not fungus-like yields PTC-resistant selectants. The DNA fragment which carries the resistance gene is obtained from the complete DNA of these selectants by constructing a gene bank and screening for chemical modification of PTC. The resistance gene can be localized to a fragment which is 2 kb in size, and selection for PTC resistance. This gene is suitable for producing PTC-resistant plants and propagation material thereof, and as a resistance marker as well. Microorganisms which contain this PTC-resistance gene can be used in sewage treatment plants.
摘要翻译：非真菌类细菌的次氯氰菊酯（PTC）选择产生耐PTC抗性选择剂。携带抗性基因的DNA片段通过构建基因库并筛选PTC的化学修饰从这些选择物的完整DNA获得。抗性基因可以定位于2kb大小的片段，并选择PTC抗性。该基因适用于生产耐PTC的植物及其繁殖材料，也可用作抗性标记。含有该PTC抗性基因的微生物可用于污水处理厂。

25. 发明申请

US20090010782A1 Fan Unit, Particularly for a Vacuum Cleaner 审中-公开
标题翻译：风机单元，特别适用于吸尘器
公开(公告)号：US20090010782A1
公开(公告)日：2009-01-08
申请号：US11909506
申请日：2006-02-23
申请人： Klaus Bartsch , Manfred Gerhards , Friedhelm Sicken
发明人： Klaus Bartsch , Manfred Gerhards , Friedhelm Sicken
IPC分类号： F04B17/03
CPC分类号： H02K7/14 , H02K5/225 , H02K11/044 , H02K11/33
摘要： A vacuum cleaner fan with an electric drive motor having a stator assembly and a rotor. The stator assembly and rotor are disposed in a bearing pot. An at least single-stage fan unit is driven by the electric motor. The fan unit includes a guide stage, a suction cap, and an impeller that is configured to generate an air stream. The bearing pot includes a receiving pocket which holds an adapter housing. The adapter housing accommodates a printed circuit board. A power semiconductor operable to control rotational speed of the fan unit is included on the printed circuit board. At least a portion of the power semiconductor protrudes from the adapter housing into the vicinity of the air stream generated by the fan unit.
摘要翻译：一种具有电驱动马达的真空吸尘器风扇，其具有定子组件和转子。定子组件和转子设置在轴承座中。至少单级风扇单元由电动机驱动。风扇单元包括引导台，吸入盖和被构造成产生空气流的叶轮。轴承座包括容纳适配器壳体的接收口袋。适配器外壳容纳印刷电路板。用于控制风扇单元的转速的功率半导体被包括在印刷电路板上。功率半导体的至少一部分从适配器壳体突出到由风扇单元产生的气流附近。

26. 发明申请

US20070265164A1 Active Substances for Increasing Pathogenic Defense in Plants and Methods for the Detection Thereof 审中-公开
标题翻译：用于增加植物病原防御的活性物质及其检测方法
公开(公告)号：US20070265164A1
公开(公告)日：2007-11-15
申请号：US11632825
申请日：2005-07-07
申请人： Klaus Bartsch , Arno Schulz
发明人： Klaus Bartsch , Arno Schulz
IPC分类号： A01N25/32 , A01N37/18 , A01N43/40 , A01N43/647 , A01P21/00 , C12Q1/68
CPC分类号： G01N33/5097 , C12Q1/6895 , C12Q2600/158
摘要： The invention relates to a method for finding compounds which induce plant pathogen defense, the enhanced expression of individual, or a plurality of, endogenous plant genes from the group consisting of jasmonic acid biosynthesis, plant proteinase inhibitors, plant xylanase inhibitors, plant PR proteins (pathogen-related proteins) and plant chitinases being regarded as a sign that induction has taken place, and to the use of these compounds alone or in combination with known compounds which act specifically and directly against phytopathogens, it being possible to carry out the application either simultaneously or staggered.
摘要翻译：本发明涉及一种从茉莉酸生物合成，植物蛋白酶抑制剂，植物木聚糖酶抑制剂，植物PR蛋白（（（（（（（（（（（（（（（（（（（（（（（（（（（（（（（（（（（（（（（（病原体相关蛋白）和植物几丁质酶被认为是诱导发生的征兆，以及单独使用这些化合物或与特异性和直接作用于植物病原体的已知化合物组合使用，可以实施同时或交错

27. 发明授权

US06686181B1 Method for the production of L-amino acids from their racemic N-aceytyl-D, L-derivatives by enzymatic racemate cleavage by means of isolated recombinant enzymes 有权
标题翻译：通过分离的重组酶通过酶促外消旋体切割从其外消旋N-乙酰基-D，L-衍生物生产L-氨基酸的方法
公开(公告)号：US06686181B1
公开(公告)日：2004-02-03
申请号：US10031356
申请日：2002-01-17
申请人： Klaus Bartsch
发明人： Klaus Bartsch
IPC分类号： C12P1304
CPC分类号： C12P41/007 , C12N9/80 , C12P13/04
摘要： Process for the preparation of L -amino acids from their racemic N-acetyl-D,L derivatives by enzymatic resolution by means of isolated, recombinant enzymes A process for the preparation of proteinogenic or nonproteinogenic L-amino acids, in particular L-phosphinothricin, from their racemic N-acetyl-D,L derivatives comprises a) selectively deacetylating N-acetyl-L derivatives of the corresponding L-amino acids by an enzymatic resolution by means of isolated, recombinant enzymes, while N-acetyl-D derivatives of the corresponding D-amino acids are not deacetylated and (b) separating the deacetylated L-amino acids obtained preparatively from the nondeacetylated N-acetyl-D derivatives and/or the incompletely deacetylated N-acetyl-L derivatives.
摘要翻译：通过分离的重组酶通过酶拆分从其外消旋的N-乙酰基-D，L衍生物制备L-氨基酸的方法用于制备蛋白质或非蛋白质的L-氨基酸，特别是L-膦丝菌素的方法，其外消旋的N-乙酰基-D，L衍生物包括a）通过分离的重组酶通过酶拆分选择性地脱乙酰相应的L-氨基酸的N-乙酰基-L衍生物，而相应的D的N-乙酰基-D衍生物 - 氨基酸不脱乙酰化，（b）从非脱乙酰基N-乙酰基-D衍生物和/或不完全脱乙酰的N-乙酰基-L衍生物中制备的脱乙酰化L-氨基酸分离。

28. 发明授权

US06384304B1 Conditional sterility in wheat 失效
标题翻译：小麦条件不育
公开(公告)号：US06384304B1
公开(公告)日：2002-05-07
申请号：US09418817
申请日：1999-10-15
申请人： Jürgen Quandt , Klaus Bartsch , Nathalie Knittel
发明人： Jürgen Quandt , Klaus Bartsch , Nathalie Knittel
IPC分类号： C12N1582
CPC分类号： C12N15/8213 , C12N9/14 , C12N15/8209 , C12N15/8231 , C12N15/8275 , C12N15/8289
摘要： The invention relates to the use of a deacetylase coding sequence for obtaining conditional sterility in wheat. The invention relates to vectors comprising a deacetylase coding sequence under control of promoters which direct stamen-selective expression in wheat, which are particularly suited for the production of wheat plants which can be made male-sterile upon application of an acetylated toxin.
摘要翻译：本发明涉及脱乙酰酶编码序列在小麦中获得条件不育性的用途。本发明涉及包含在小麦中直接雄蕊选择性表达的启动子控制下的脱乙酰酶编码序列的载体，其特别适用于在施用乙酰化毒素时可以制备为雄性不育的小麦植物。

29. 发明授权

US06335186B1 Process for the preparation of L-phosphinothricin by a coupled enzymatic reaction 失效
标题翻译：通过偶联酶反应制备L-膦丝菌素的方法
公开(公告)号：US06335186B1
公开(公告)日：2002-01-01
申请号：US08408178
申请日：1995-03-22
申请人： Klaus Bartsch , Gerd Fülling , Arno Schulz
发明人： Klaus Bartsch , Gerd Fülling , Arno Schulz
IPC分类号： C12P1300
CPC分类号： C12P13/14 , C12P7/46 , C12P13/04
摘要： A coupled enzyme reaction of a transaminase with GOT activity and of a transaminase with L-phosphinothricin transaminase activity produces phosphinothricin in virtually quantitative yield and virtually without any contamination whatever by a natural amino acid when the amino donor glutamate is employed in catalytic amounts and the amino donor aspartate is employed in approximately equimolar amounts relative to 4-(hydroxymethylphosphinyl)-2-oxobutyric acid.
摘要翻译：具有GOT活性的转氨酶和具有L-膦丝菌素转氨酶活性的转氨酶的偶联酶反应在氨基供体谷氨酸盐以催化量使用时产生实质上定量产率的氯氰菊酯，并且几乎不受任何天然氨基酸的任何污染，氨基供体天冬氨酸以相对于4-（羟甲基氧膦基）-2-氧代丁酸大约等摩尔量使用。

30. 发明授权

US06177616B1 Genes coding for amino acid deacetylases with specificity for N-acetyl-L-phosphinothricin, their isolation and their use 失效
标题翻译：编码具有N-乙酰基-L-膦丝菌素特异性的氨基酸脱乙酰酶的基因，其分离及其用途
公开(公告)号：US06177616B1
公开(公告)日：2001-01-23
申请号：US09319892
申请日：1999-06-14
申请人： Klaus Bartsch , Guido Kriete , Inge Broer , Alfred P{umlaut over (u)}hler
发明人： Klaus Bartsch , Guido Kriete , Inge Broer , Alfred P{umlaut over (u)}hler
IPC分类号： C12N1505
CPC分类号： C12R1/01 , C12N1/20 , C12N9/14 , C12N15/8209 , C12N15/8289
摘要： The invention relates to DNA molecules which code for deacetylases or proteins having the biological activity of a deacetylase and also transgenic plant cells which were transformed using the DNA molecules according to the invention. These molecules can be used for the production of plants having specifically destroyable parts, i.e. male- or female-sterile plants, by means of the specific expression of a deacetylase gene.
摘要翻译：本发明涉及编码具有脱乙酰酶生物活性的脱乙酰酶或蛋白质的DNA分子，以及使用根据本发明的DNA分子转化的转基因植物细胞。这些分子可以通过脱乙酰酶基因的特异性表达用于生产具有特异性可破坏部分的植物，即雄性或雌性不育植物。

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