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21. 发明授权

US07880149B2 3D biplane microscopy 失效
标题翻译： 3D双平面显微镜
公开(公告)号：US07880149B2
公开(公告)日：2011-02-01
申请号：US12826422
申请日：2010-06-29
申请人： Joerg Bewersdorf , Manuel F. Juette , Travis Gould , Sam T. Hess
发明人： Joerg Bewersdorf , Manuel F. Juette , Travis Gould , Sam T. Hess
IPC分类号： G01J1/58 , G02B21/06
CPC分类号： G02B21/367 , G02B21/16 , G02B21/361 , G02B27/58 , H04N13/218 , H04N13/254 , H04N2013/0085
摘要： A microscopy system is configured for creating 3D images from individually localized probe molecules. The microscopy system includes a sample stage, an activation light source, a readout light source, a beam splitting device, at least one camera, and a controller. The activation light source activates probes of at least one probe subset of photo-sensitive luminescent probes, and the readout light source causes luminescence light from the activated probes. The beam splitting device splits the luminescence light into at least two paths to create at least two detection planes that correspond to the same or different number of object planes of the sample. The camera detects simultaneously the at least two detection planes, the number of object planes being represented in the camera by the same number of recorded regions of interest. The controller is programmable to combine a signal from the regions of interest into a 3D data.
摘要翻译：配置显微镜系统以从单独定位的探针分子创建3D图像。显微镜系统包括样品台，激活光源，读出光源，分束装置，至少一个照相机和控制器。活化光源激活光敏发光探针的至少一个探针子集的探针，并且读出的光源引起来自激活的探针的发光。分束装置将发光分成至少两条路径，以产生对应于样品的相同或不同数目的物平面的至少两个检测平面。相机同时检测至少两个检测平面，相机中相同数量的记录感兴趣区域的对象平面数目被表示在相机中。控制器是可编程的，以将来自感兴趣区域的信号组合成3D数据。

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