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    • 29. 发明授权
    • Polypeptides having xylanase activity from Aspergillus fumigatus
    • 具有来自烟曲霉的木聚糖酶活性的多肽
    • US07960160B2
    • 2011-06-14
    • US11054191
    • 2005-02-09
    • Debbie YaverPaul HarrisSuzanne OtaniJanine LinHaiyan Ge
    • Debbie YaverPaul HarrisSuzanne OtaniJanine LinHaiyan Ge
    • C12N9/24C12N15/56
    • C12N9/2482C12N9/248C12Y302/01008
    • The present invention relates to an isolated polypeptide having xylanase activity from Aspergillus fumigatus with a molecular weight of approximately 50 kDa by SDS-PAGE and homologous polypeptides thereof comprising an amino acid sequence having at least 95% identity with amino adds 20 to 397 of SEQ ID NO: 6; encoded by a polynucleotide comprising a nucleotide sequence having at least 95% identity with nucleotides 107 to 1415 of SEQ ID NO: 5: or encoded by a polynucleotide that hybridizes under high stringency conditions with (i) nucleotides 107 to 1415 of SEQ ID NO: 5, (ii) the cDNA sequence of (i), or (iii) a full-length complementary strand of (i) or (ii). The present invention also relates to a method for producing such polypeptides having xylanase activity comprising: (a) cultivating a wild-type Aspergillus fumigatus cell under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide.
    • 本发明涉及通过SDS-PAGE分析重量为约50kDa的烟曲霉具有木聚糖酶活性的分离多肽,并且其同源多肽包含与氨基具有至少95%同一性的氨基酸序列,添加SEQ ID NO:20至397 NO:6; 由包含与SEQ ID NO:5的核苷酸107至1415具有至少95%同一性的核苷酸序列的多核苷酸编码,或由在高严格条件下与(i)SEQ ID NO:5的核苷酸107至1415杂交的多核苷酸编码。 5,(ii)(i)或(iii)(i)或(ii)的全长互补链的cDNA序列。 本发明还涉及生产具有木聚糖酶活性的多肽的方法,其包括:(a)在有利于产生多肽的条件下培养野生型烟曲霉细胞; 和(b)回收多肽。