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    • 24. 发明授权
    • Pectin lyase, pectin lyase polynucleotide, enzyme preparation, and method for producing single cells of plant tissue
    • 果胶裂解酶,果胶裂解酶多核苷酸,酶制剂和用于生产植物组织单细胞的方法
    • US08691536B2
    • 2014-04-08
    • US13502869
    • 2010-07-15
    • Yoshihisa NakanoMitsuhiro UenoyamaErina Kobayashi
    • Yoshihisa NakanoMitsuhiro UenoyamaErina Kobayashi
    • C12P19/14C12N9/88C12N15/00C07H21/04
    • C12N9/88A01N63/04C12P19/00C12Y402/0201
    • To provide a pectin lyase and a gene for a pectin lyase which can be used in an enzyme preparation for processing plant tissue, and which possesses sufficient maceration activity even under highly acidic conditions. Isolation and purification of a pectin lyase and a gene for a pectin lyase having a molecular weight of 39,500 produced by an Aspergillus filamentous fungus, and possessing a maceration activity and a pectin lyase activity. The pectin lyase has a specified amino acid sequence, an optimal pH of 3.5 for maceration activity, an optimal pH of 4.5 for pectin lyase activity, and deactivates with boiling treatment for 15 minutes at 121° C. Sterilizing heat treatment are unnecessary, because the present invention possesses sufficient maceration activity at pH 3.0-4.0, and the resulting pectin lysate possesses a bactericidal activity and a potent antibacterial activity. It is possible to inexpensively mass produce food products which retain water-soluble components within the cell, because physical treatment such as stirring or beating is also unnecessary.
    • 提供可用于加工植物组织的酶制剂中的果胶裂解酶和果胶裂解酶的基因,即使在高酸性条件下也具有足够的浸渍活性。 分离和纯化果胶裂解酶和由丝状真菌产生的分子量为39,500的果胶裂解酶的基因,并具有浸渍活性和果胶裂解酶活性。 果胶裂解酶具有特定的氨基酸序列,浸出活性的最适pH为3.5,果胶裂解酶活性的最佳pH为4.5,在121℃下沸腾处理15分钟后失活。不需要灭菌热处理,因为 本发明在pH3.0-4.0时具有足够的浸渍活性,所得果胶裂解物具有杀菌活性和强力的抗菌活性。 由于不需要诸如搅拌或打浆的物理处理,因此可以廉价地大量生产保留细胞内的水溶性成分的食品。
    • 25. 发明申请
    • Pectin Lyase, Pectin Lyase Gene, Enzyme Preparation, and Method for Producing Single Cells of Plant Tissue
    • 果胶裂解酶,果胶裂解酶基因,酶制备和生产植物组织单细胞的方法
    • US20120220016A1
    • 2012-08-30
    • US13502869
    • 2010-07-15
    • Yoshihisa NakanoMitsuhiro UenoyamaErina Kobayashi
    • Yoshihisa NakanoMitsuhiro UenoyamaErina Kobayashi
    • C12N9/88C12N5/04C07H21/04
    • C12N9/88A01N63/04C12P19/00C12Y402/0201
    • To provide a pectin lyase and a gene for a pectin lyase which can be used in an enzyme preparation for processing plant tissue, and which possesses sufficient maceration activity even under highly acidic conditions. Isolation and purification of a pectin lyase and a gene for a pectin lyase having a molecular weight of 39,500 produced by an Aspergillus filamentous fungus, and possessing a maceration activity and a pectin lyase activity. The pectin lyase has a specified amino acid sequence, an optimal pH of 3.5 for maceration activity, an optimal pH of 4.5 for pectin lyase activity, and deactivates with boiling treatment for 15 minutes at 121° C. Sterilizing heat treatment are unnecessary, because the present invention possesses sufficient maceration activity at pH 3.0-4.0, and the resulting pectin lysate possesses a bactericidal activity and a potent antibacterial activity. It is possible to inexpensively mass produce food products which retain water-soluble components within the cell, because physical treatment such as stirring or beating is also unnecessary.
    • 提供可用于加工植物组织的酶制剂中的果胶裂解酶和果胶裂解酶的基因,即使在高酸性条件下也具有足够的浸渍活性。 分离和纯化果胶裂解酶和由丝状真菌产生的分子量为39,500的果胶裂解酶的基因,并具有浸渍活性和果胶裂解酶活性。 果胶裂解酶具有特定的氨基酸序列,浸出活性的最适pH为3.5,果胶裂解酶活性的最佳pH为4.5,在121℃下沸腾处理15分钟后失活。不需要灭菌热处理,因为 本发明在pH3.0-4.0时具有足够的浸渍活性,所得果胶裂解物具有杀菌活性和强力的抗菌活性。 由于不需要诸如搅拌或打浆的物理处理,因此可以廉价地大量生产保留细胞内的水溶性成分的食品。
    • 28. 发明申请
    • DATABASE MANAGEMENT METHOD AND DATABASE MANAGEMENT APPARATUS
    • 数据库管理方法和数据库管理装置
    • US20080222209A1
    • 2008-09-11
    • US12039443
    • 2008-02-28
    • Yoshihisa NakanoTakayuki NakazawaNaohiro Ito
    • Yoshihisa NakanoTakayuki NakazawaNaohiro Ito
    • G06F17/30
    • G06F17/30315
    • According to an aspect of an embodiment, a method for managing a database for records of transactions, each of the transaction specifying an order of processing, and being associated with a value related to generation of the transaction, said database comprising a plurality of page data containing a plurality of records, each of the records comprising data and information indicative of the value of one of said transactions, the method comprising the steps of: reading out one of said page data containing a target record of request of a transaction associated with the target record from said database; evaluating each value associated with the transactions in the page data; and invaliding the record associated with a value greater than a predetermined value.
    • 根据实施例的一个方面,一种用于管理用于事务记录的数据库的方法,每个事务指定处理的顺序,并且与与事务的生成有关的值相关联,所述数据库包括多个页面数据 包含多个记录,每个记录包括指示所述交易之一的数据和信息的信息,所述方法包括以下步骤:读出包含与所述交易相关联的交易的请求的目标记录的所述页面数据之一 来自所述数据库的目标记录; 评估与页面数据中的交易相关联的每个值; 并且使得与大于预定值的值相关联的记录无效。