专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

21. 发明授权

US5830725A Rapid, stable high-titre production of recombing retrovirus 失效
标题翻译：快速，稳定的高滴度生产推荐逆转录病毒
公开(公告)号：US5830725A
公开(公告)日：1998-11-03
申请号：US430813
申请日：1995-04-28
申请人： Garry P. Nolan , Todd Kinsella
发明人： Garry P. Nolan , Todd Kinsella
IPC分类号： C12N15/867 , C12N15/00 , A61K48/00 , C07K14/00 , C12N5/00
CPC分类号： C12N15/86 , C12N2740/13043
摘要： High titre helper-free recombinant retrovirus are produced by (a) growing a transfected host cell, produced by transfecting a eukaryotic host cell with a recombinant vector capable of stable episomal maintenance in the host cell, in a medium under conditions whereby the recombinant vector is stably maintained as an episome in the transfected host cell and transcripts of said vector form, with retroviral gag, pol and env gene products, an infectious retrovirus; and (b) isolating from the medium helper-free infectious retrovirus formed in the transfected host cell. The recombinant vectors comprise (i) a retroviral construct comprising an exogenous gene; (ii) a eukaryotic origin of replication sequence providing a substrate for replicase activity capable of replicating the vector in the host cell; and, (iii) a copy control sequence providing a substrate for a copy control activity capable of maintaining the vector at a stable copy number in the host cell. The transfected host cell provides retroviral pol reverse transcriptase and integrase activity, the replicase activity and copy control activity, as well as retroviral gag and env gene products capable of forming, with a transcript of the retroviral construct, an infectious retrovirus.
摘要翻译：通过以下步骤制备高滴度无辅助重组逆转录病毒：（a）通过在培养基中培养转染的宿主细胞，所述转染的宿主细胞通过在宿主细胞中能够稳定的附加型维持的重组载体转染真核宿主细胞而产生，在转染的宿主细胞中稳定地维持该转录物和所述载体形式的转录物，其具有逆转录病毒gag，pol和env基因产物，感染性逆转录病毒; 和（b）从转染的宿主细胞中形成的中等无辅助感染性逆转录病毒分离。重组载体包含（i）包含外源基因的逆转录病毒构建体; （ii）提供能够复制宿主细胞中的载体的复制酶活性的底物的真核复制起点序列; 和（iii）复制控制序列，其提供能够将载体维持在宿主细胞中稳定拷贝数的拷贝控制活性的底物。转染的宿主细胞提供逆转录病毒pol逆转录酶和整合酶活性，复制酶活性和拷贝对照活性，以及能够与逆转录病毒构建体的转录物形成感染性逆转录病毒的逆转录病毒gag和env基因产物。

22. 发明申请

US20050227291A1 Heterocyclic compounds containing a nine-membered carbon-nitrogen ring 有权
标题翻译：含九元碳氮环的杂环化合物
公开(公告)号：US20050227291A1
公开(公告)日：2005-10-13
申请号：US11033416
申请日：2005-01-10
申请人： Todd Kinsella
发明人： Todd Kinsella
IPC分类号： C07H21/04 , C07K5/12 , C12N15/09 , C12P17/10 , C12P21/02 , C12P21/06 , G01N33/53
CPC分类号： C07K5/123 , C12P17/10 , C12P21/02
摘要： The present invention provides heterocyclic compounds having a nine-membered ring of three repeating C—C—N subunits covalently bound to each other through amide bonds, and variable side groups linked to a central C of each subunit. Also described herein are cells containing the cyclic compounds, methods of screening those cells to identify a cyclic compound of interest, and libraries of cyclic compounds and their encoding nucleic acids. The subject compounds may be employed in a variety of research and medical applications, including methods of treating a patient for hepatitis C infection.
摘要翻译：本发明提供具有通过酰胺键彼此共价结合的三个重复C-C-N亚基的九元环的杂环化合物，以及与各亚基的中心C连接的可变侧基。本文还描述了含有环状化合物的细胞，筛选这些细胞以鉴定感兴趣的环状化合物的方法，以及环状化合物及其编码核酸的文库。主题化合物可用于各种研究和医学应用，包括治疗患者丙型肝炎感染的方法。

23. 发明申请

US20050158820A1 In vivo production of cyclic peptides 有权
标题翻译：体内生成环肽
公开(公告)号：US20050158820A1
公开(公告)日：2005-07-21
申请号：US11011880
申请日：2004-12-13
申请人： Todd Kinsella
发明人： Todd Kinsella
IPC分类号： C12N15/10 , C12P21/06 , C07K5/12 , C12N15/867
CPC分类号： C12N15/1055 , C12N15/10
摘要： The present invention relates ti methods and compositions utilizing inteins to generated libraries of cyclic peptides in vivo.
摘要翻译：本发明涉及利用内含肽在生物体内生成环肽文库的方法和组合物。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式