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    • 22. 发明申请
    • System for cell culture
    • 细胞培养系统
    • US20070128718A1
    • 2007-06-07
    • US10595890
    • 2004-11-18
    • Didier CourtoisArnaud CuvierNicolas Henault
    • Didier CourtoisArnaud CuvierNicolas Henault
    • C12N5/06C12N5/04C12M3/00
    • C12M23/14C12M23/50C12M27/16
    • The present invention provides a novel apparatus to grow cells or microorganisms where the cultivation chamber is partially filled with liquid cultivation medium and cells. The wave induction mechanism lifts up between 5 and 50 % of the length of the culture chamber. Mixing and aeration is achieved by inducing intermittently waves from one extremity of the bioreactor to the other. As the design of the invention is very simple, it is possible to manufacture it with flexible plastic material and use the apparatus as a disposable system. Moreover, such a mixing/aeration principle minimizes cell damages usually due to shear stress and small bubbles. Since the waves induction mechanism is very simple, it allows easy and efficient scale-up from small scale to a larger one. Such a large-scale, efficient and disposable culture system can largely reduce production costs.
    • 本发明提供一种用于培养细胞或微生物的新型装置,其中培养室部分地装有液体培养基和细胞。 波感应机构提升培养室长度的5至50%。 通过从生物反应器的一个末端间歇地诱导波浪到另一个末端实现混合和曝气。 由于本发明的设计非常简单,所以可以用柔性塑料制造它,并将该装置用作一次性系统。 此外,这种混合/曝气原理通常由于剪切应力和小气泡而使细胞损伤最小化。 由于波感应机构非常简单,因此允许从小规模到较大尺寸的放大容易而有效。 这种大规模,高效和一次性的文化体系可以大大降低生产成本。