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21. 发明公开

EP0571500A1 A HIGH SULFUR SEED PROTEIN GENE AND METHOD FOR INCREASING THE SULFUR AMINO ACID CONTENT OF PLANTS 失效
标题翻译：富硫蛋白基因种子和方法提高硫含AMINOSAEURENGEHALTES的抗性。
公开(公告)号：EP0571500A1
公开(公告)日：1993-12-01
申请号：EP92905689.0
申请日：1992-02-14
申请人： E.I. DU PONT DE NEMOURS AND COMPANY
发明人： CHUI, Chok-Fun, Chan , FALCO, Saverio, Carl , RICE, Janet, Ann , KNOWLTON, Susan 410 Casparus Way
IPC分类号： A01H1 , A01H5 , C07K14 , C12N15 , C12P21
CPC分类号： C12N15/8253 , C07K14/415 , C07K14/425 , C12N15/8251
摘要： On décrit un nouveau fragment d'acide nucléique utilisé pour la surexpression d'une protéine de réserve d'une graine à teneur élevée en méthionine chez les plantes. Le fragment d'acide nucléique peut transformer des plantes, en particulier des plantes de culture, de manière à surexprimer une protéine de réserve de graines de maïs dans les graines ou les feuilles. L'invention présente un intérêt particulier en ce qui concerne l'amélioration des qualités nutritives de plantes pauvres en acide aminé soufré, telles que le maïs ou le soja. On décrit aussi le produit polypeptidique des fragments d'acide nucléique ainsi que des gènes chimères, des cellules hôtes, des plantes, des graines et des micro-organismes contenant le fragment d'acide nucléique. Des procédés servant à produire la surexpression de la protéine de réserve d'une graine dans les micro-organismes sont aussi décrits.

22. 发明授权

EP1002113B1 PLANT AMINO ACID BIOSYNTHETIC ENZYMES 失效
标题翻译：植物酶氨基酸生物合成
公开(公告)号：EP1002113B1
公开(公告)日：2009-03-11
申请号：EP98926537.6
申请日：1998-06-11
申请人： E.I. DU PONT DE NEMOURS AND COMPANY
发明人： FALCO, Saverio, Carl , ALLEN, Stephen, M. , THORPE, Catherine, Jane
IPC分类号： C12N15/82 , C12N9/02 , C12N9/88 , C12N9/12 , C12Q1/68 , G01N33/50 , A01H5/00
CPC分类号： C12N9/0008 , C12N9/1205 , C12N9/88 , C12N15/8251 , C12N15/8253 , C12N15/8254

23. 发明公开

EP1151084A2 PLANT POLYPHENOL OXIDASE HOMOLOGS 审中-公开
标题翻译：多酚氧化酶同源植物
公开(公告)号：EP1151084A2
公开(公告)日：2001-11-07
申请号：EP00911722.7
申请日：2000-02-08
申请人： E.I. DU PONT DE NEMOURS AND COMPANY
发明人： CAHOON, Rebecca, E. , FALCO, Saverio, Carl , KINNEY, Anthony, J. , MIAO, Guo-Hua
IPC分类号： C12N9/02 , C12N15/53 , A01H5/00 , C12N15/82 , G01N33/50 , C12N15/10 , C12Q1/68
CPC分类号： C12N9/0059 , C12N9/0061 , C12N15/8242 , C12N15/8243
摘要： This invention relates to an isolated nucleic acid fragment encoding a polyphenol oxidase enzyme. The invention also relates to the construction of a chimeric gene encoding all or a portion of the polyphenol oxidase enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the polyphenol oxidase enzyme in a transformed host cell.

24. 发明公开

EP1127144A2 PLANT GLUTAMINE AMIDOTRANSFERASE HOMOLOGS 审中-公开
标题翻译：植物谷氨酰胺酰胺转移酶同源序列
公开(公告)号：EP1127144A2
公开(公告)日：2001-08-29
申请号：EP99956903.1
申请日：1999-11-04
申请人： E.I. DU PONT DE NEMOURS AND COMPANY
发明人： ALLEN, Stephen, M. , HUANG, Lisa, L. , FALCO, Saverio, Carl , RAFALSKI, Antoni, J.
IPC分类号： C12N15/82 , C12N15/52 , C12N9/00 , C12N5/10 , G01N33/50 , C12Q1/68
CPC分类号： C12N9/93 , C12N15/8243
摘要： This invention relates to an isolated nucleic acid fragment encoding a histidine biosynthetic enzyme. The invention also relates to the construction of a chimeric gene encoding all or a portion of the histidine biosynthetic enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the histidine biosynthetic enzyme in a transformed host cell.

25. 发明公开

EP1097222A1 TETRAHYDROFOLATE METABOLISM ENZYMES 审中-公开
标题翻译：四氢叶酸代谢酶
公开(公告)号：EP1097222A1
公开(公告)日：2001-05-09
申请号：EP99935558.9
申请日：1999-07-14
申请人： E.I. DU PONT DE NEMOURS AND COMPANY
发明人： FALCO, Saverio, Carl , FOMODU, Layo, O.
IPC分类号： C12N15/53 , C12N9/06 , C12N5/10 , C12Q1/68
CPC分类号： C12N9/0044
摘要： This invention relates to an isolated nucleic acid fragment encoding a tetrahydrofolate metabolism enzyme. The invention also relates to the construction of a chimeric gene encoding all or a portion of the tetrahydrofolate metabolism enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the tetrahydrofolate metabolism enzyme in a transformed host cell.
摘要翻译：本发明涉及编码四氢叶酸代谢酶的分离的核酸片段。本发明还涉及以正义或反义方向构建编码全部或部分四氢叶酸代谢酶的嵌合基因，其中嵌合基因的表达导致在转化的宿主细胞中产生改变水平的四氢叶酸代谢酶。

26. 发明授权

EP0571500B1 A HIGH SULFUR SEED PROTEIN GENE AND METHOD FOR INCREASING THE SULFUR AMINO ACID CONTENT OF PLANTS 失效
标题翻译：富硫蛋白基因种子和方法提高硫含AMINOSAEURENGEHALTES植物中
公开(公告)号：EP0571500B1
公开(公告)日：2000-09-13
申请号：EP92905689.3
申请日：1992-02-14
申请人： E.I. DU PONT DE NEMOURS AND COMPANY
发明人： CHUI, Chok-Fun, Chan , FALCO, Saverio, Carl , RICE, Janet, Ann , KNOWLTON, Susan 410 Casparus Way
IPC分类号： C12N15/29 , C12N15/82 , A01H5/00 , C07K14/00 , C12P21/02
CPC分类号： C12N15/8253 , C07K14/415 , C07K14/425 , C12N15/8251
摘要： There is provided a novel nucleic acid fragment for the overexpression of a high methionine seed storage protein in plants. This nucleic acid fragment is capable of transforming plants, particularly crop plants, to overexpress a corn seed storage protein in seeds or leaves. The invention is of significant interest for the nutritional improvement of sulfur amino acid-poor plants, such as corn and soybean. There is also provided the polypeptide product of the nucleic acid fragments as well as chimeric genes, host cells, plants, seeds and microorganisms containing the nucleic acid fragment. Methods for obtaining the overexpression of the seed storage protein in microorganisms are also provided.

27. 发明公开

EP0734445A1 CHIMERIC GEENS AND METHODS FOR INCREASING THE LYSINE CONTENT OF THE SEEDS OF CORN, SOYBEAN AND RAPESEED PLANTS 失效
标题翻译：嵌合基因和方法的玉米，大豆和油菜植株的种子增加赖氨酸含量
公开(公告)号：EP0734445A1
公开(公告)日：1996-10-02
申请号：EP95903539.0
申请日：1994-11-21
申请人： E.I. DU PONT DE NEMOURS AND COMPANY
发明人： FALCO, Saverio, Carl , KEELER, Sharon, Jo , RICE, Janet, Ann
IPC分类号： A01H5 , C07K14 , C12N5 , C12N9 , C12N15
CPC分类号： C07K14/415 , C07K14/00 , C12N9/12 , C12N9/88 , C12N15/8254
摘要： This invention relates to three chimeric genes, the first encoding dihydrodipicolinic acid synthase (DHDPS), which is insensitive to inhibition by lysine and operably linked to a plant chloroplast transit sequence, a second encoding a lysine-rich protein, and a third encoding a plant lysine ketoglutarate reductase, all operably linked to plant seed-specific regulatory sequences. Methods for their use to produce increased levels of lysine in the seeds of transformed plants are provided. Also provided are transformed corn, rapeseed and soybean plants wherein the seeds accumulate lysine to higher levels than untransformed plants.

28. 发明申请

WO2010080430A1 SITE-SPECIFIC INTEGRATION AND STACKING OF TRANSGENES IN SOYBEAN VIA DNA RECOMBINASE MEDIATED CASSETTE EXCHANGE 审中-公开
标题翻译：通过DNA重组体介导的CASSETTE交换的大豆的特异性整合和转化大肠杆菌
公开(公告)号：WO2010080430A1
公开(公告)日：2010-07-15
申请号：PCT/US2009/068215
申请日：2009-12-16
申请人： E. I. DU PONT DE NEMOURS AND COMPANY , FALCO, Saverio, Carl , LI, Zhongsen
发明人： FALCO, Saverio, Carl , LI, Zhongsen
IPC分类号： C12N15/82 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8213
摘要： A targeting method is described that allows precise cassette replacement at a previously characterized genetic locus. A target DNA construct containing a pair of incompatible FRT sites flanking a target cassette was introduced into soybean by regular biolistic transformation. Transgenic events containing a single complete copy of the target site were then selected and retransformed with a donor DNA construct containing the identical pair of incompatible FRT sites flanking a donor cassette. Precise DNA cassette exchange happened between the target cassette and the donor cassette via recombinase mediated cassette exchange (RMCE) so that the donor cassette was introduced at the exact genomic site previously occupied by the target cassette. Through repeated RMCE using additional incompatible FRT sites, multiple groups of transgenes can be stacked at the same genomic locus.
摘要翻译：描述了靶向方法，其允许在先前表征的遗传基因座处进行精确的盒替换。通过常规的生物弹性转化，将含有目标盒侧翼的一对不相容的FRT位点的靶DNA构建体引入大豆。然后选择含有单个完整拷贝的靶位点的转基因事件，并用含有供体盒侧翼的相同对不对称FRT位点的供体DNA构建体重新转化。通过重组酶介导的盒式交换（RMCE）在靶盒和供体盒之间发生精确的DNA盒交换，使得供体盒被导入到由靶盒预先占据的确切基因组位点。通过反复RMCE使用额外的不相容的FRT位点，可以在相同的基因组座位堆叠多组转基因。

29. 发明申请

WO2006076717A2 A NOVEL CYTOCHROME P450 MONOOXYGENASE 审中-公开
标题翻译：新的CYTOCHROME P450 MONOOXYGENASE
公开(公告)号：WO2006076717A2
公开(公告)日：2006-07-20
申请号：PCT/US2006/001549
申请日：2006-01-13
申请人： E.I. DUPONT DE NEMOURS AND COMPANY , MCGONIGLE, Brian , FALCO, Saverio, Carl , EVERARD, John, D. , SWINSON, Nicole, A.
发明人： MCGONIGLE, Brian , FALCO, Saverio, Carl , EVERARD, John, D. , SWINSON, Nicole, A.
CPC分类号： C12N9/0077 , C12N15/8243 , C12N15/8247 , C12P7/04
摘要： This invention relates to an isolated polynucleotide encoding a cytochrome P450 monooxygenase that in the presence of linoleic acid results in production of 1-octen-3-ol. The invention also relates to the construction of a recombinant DNA construct comprising all or a portion of the polynucleotide encoding the cytochrome P450 monooxygenases of the invention, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels 1-octen-3-ol in a transformed host cell.
摘要翻译：本发明涉及编码细胞色素P450单加氧酶的分离的多核苷酸，其在亚油酸存在下导致1-辛烯-3-醇的生产。本发明还涉及在正义或反义方向上构建包含编码本发明的细胞色素P450单加氧酶的全部或部分多核苷酸的重组DNA构建体，其中重组DNA构建体的表达导致产生改变的水平1- 辛烯-3-醇在转化的宿主细胞中。

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