专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

21. 发明授权

US06599909B1 Molecular chemotherapy enhancement of radiotherapy 失效
标题翻译：分子化疗放疗增强
公开(公告)号：US06599909B1
公开(公告)日：2003-07-29
申请号：US09408055
申请日：1999-09-29
申请人： Donald J. Buchsbaum , David T. Curiel , Murray A. Stackhouse , Lee C. Pederson
发明人： Donald J. Buchsbaum , David T. Curiel , Murray A. Stackhouse , Lee C. Pederson
IPC分类号： A61K31505
CPC分类号： C12N15/86 , A61K38/45 , A61K38/50 , A61K41/00 , A61K48/00 , C12N2710/10343 , C12N2810/859 , Y02A50/473
摘要： The present invention provides a new approach for cancer treatment by utilizing gene therapy combined with radiation therapy to enhance cytotoxicity in malignant cells. Specifically, the present invention demonstrates that molecular chemotherapy with the cytosine deaminase gene and 5-fluorocytosine is an effective radiosensitizing strategy which may lead to substantial improvement in tumor control, with less normal tissue toxicity than conventional systemic administration of 5-fluorouracil, that would translate into improved cure rates and better survival. Also provided is a noninvasive method for continuous in vivo monitoring of 5-fluorouracil production via magnetic resonance spectroscopy.
摘要翻译：本发明通过利用与放射治疗相结合的基因治疗来增强恶性细胞中的细胞毒性提供了一种新的癌症治疗方法。具体来说，本发明表明，具有胞嘧啶脱氨酶基因和5-氟胞嘧啶的分子化学疗法是一种有效的放射增敏策略，其可以导致肿瘤控制的显着改善，具有比常规全身施用5-氟尿嘧啶更少的正常组织毒性，这将翻译改善治愈率和更好的生存。还提供了一种用于通过磁共振光谱法连续体内监测5-氟尿嘧啶生产的非侵入性方法。

22. 发明授权

US06348450B1 Noninvasive genetic immunization, expression products therefrom and uses thereof 有权
标题翻译：无创遗传免疫，其表达产物及其用途
公开(公告)号：US06348450B1
公开(公告)日：2002-02-19
申请号：US09563826
申请日：2000-05-03
申请人： De-chu C. Tang , Donald H. Marks , David T. Curiel , Zhongkai Shi , Kent Rigby van Kampen
发明人： De-chu C. Tang , Donald H. Marks , David T. Curiel , Zhongkai Shi , Kent Rigby van Kampen
IPC分类号： A61K4800
CPC分类号： A61K39/08 , A61K39/00 , A61K39/0011 , A61K39/12 , A61K39/145 , A61K48/00 , A61K2039/53 , A61K2039/54 , A61K2039/541 , A61K2039/542 , A61K2039/543 , A61K2039/55555 , A61K2039/60 , C12N2710/10343 , C12N2760/16134
摘要： Disclosed and claimed are methods of non-invasive genetic immunization in an animal and/or methods of inducing a systemic immune or therapeutic response in an animal, products therefrom and uses for the methods and products therefrom. The methods can include contacting skin of the animal with a vector in an amount effective to induce the systemic immune or therapeutic response in the animal. The vector can include and express an exogenous nucleic acid molecule encoding an epitope or gene product of interest. The systemic immune response can be to or from the epitope or gene product. The nucleic acid molecule can encode an epitope of interest and/or an antigen of interest and/or a nucleic acid molecule that stimulates and/or modulates an immunological response and/or stimulates and/or modulates expression, e.g., transcription and/or translation, such as transcription and/or translation of an endogenous and/or exogenous nucleic acid molecule; e.g., one or more of influenza hemagglutinin, influenza nuclear protein, tetanus toxin C-fragment, anthrax protective antigen, HIV gp 120, human carcinoembryonic antigen, and/or a therapeutic, an immunomodulatory gene, such as co-stimulatory gene and/or a cytokine gene. The immune response can be induced by the vector expressing the nucleic acid molecule in the animal's cells. The immune response can be against a pathogen or a neoplasm. A prophylactic vaccine or a therapeutic vaccine or an immunological composition can include the vector.
摘要翻译：公开和要求的是在动物中非侵入性遗传免疫的方法和/或在动物中诱导全身性免疫或治疗反应的方法，其产品及其用途。所述方法可以包括使动物的皮肤与有效诱导动物体内免疫或治疗反应的量的载体接触。载体可以包括并表达编码感兴趣的表位或基因产物的外源核酸分子。全身免疫应答可以来自表位或基因产物。核酸分子可以编码感兴趣的表位和/或感兴趣的抗原和/或刺激和/或调节免疫应答和/或刺激和/或调节表达的核酸分子，例如转录和/或翻译，例如内源和/或外源核酸分子的转录和/或翻译; 例如流感血凝素，流感核蛋白，破伤风毒素C片段，炭疽保护性抗原，HIV gp120，人癌胚抗原和/或治疗性免疫调节基因中的一种或多种，例如共刺激基因和/或细胞因子基因。可以通过在动物细胞中表达核酸分子的载体诱导免疫应答。免疫应答可以针对病原体或肿瘤。预防性疫苗或治疗性疫苗或免疫组合物可以包括载体。

23. 发明授权

US06312699B1 Ligands added to adenovirus fiber 失效
标题翻译：配体添加到腺病毒纤维中
公开(公告)号：US06312699B1
公开(公告)日：2001-11-06
申请号：US08218369
申请日：1994-03-28
申请人： David T. Curiel , Jeffrey A. Engler
发明人： David T. Curiel , Jeffrey A. Engler
IPC分类号： A61K3923
CPC分类号： C12N15/86 , A61K48/00 , C07K14/005 , C07K2319/00 , C12N15/87 , C12N2710/10322 , C12N2710/10343 , C12N2810/40
摘要： The fiber protein of adenovirus has been genetically altered via attachment at the carboxyl end of a peptide linker, preferably up to 26 amino acids in length which forms a random coil, which can be used to attach a non-adenovirus ligand altering the binding specificity of the fiber protein. Examples of ligands include peptides which are selectively bound by a targeted cell so that the modified fiber protein is internalized by receptor-mediated endocytosis, and peptides which can act as an universal coupling agent, for example, biotin or strepavidin. The linker is designed to not interfere with normal trimerization of fiber protein, to avoid steric hindrance of binding of the fiber protein to a targeted cell, and to serve as a site to introduce new peptide sequence. The modified fiber protein is prepared by genetic engineering of the nucleotide sequence encoding the fiber protein, through the addition of new sequence at the carboxyl tail-encoding region which encodes the linker and the ligand. The N-terminus of the fiber protein is not altered in the preferred embodiment, although in some embodiments it may be desirable to inhibit uptake by the nucleus of the fiber protein, by deletion of nuclear targeting signals. The modified fiber protein can be utilized as part of a recombinant adenovirus for use in gene therapy.
摘要翻译：腺病毒的纤维蛋白已经通过在肽接头的羧基末端的连接被遗传改变，优选长达26个氨基酸长度，其形成无规卷曲，其可用于附着非腺病毒配体，其改变结合特异性纤维蛋白。配体的实例包括被靶细胞选择性结合以使经修饰的纤维蛋白质被受体介导的内吞作用内化的肽，以及可以作为通用偶联剂例如生物素或链霉亲和素的肽。接头被设计成不干扰纤维蛋白质的正常三聚，以避免纤维蛋白质与靶细胞结合的空间位阻，并用作引入新肽序列的位点。通过在编码接头和配体的羧基尾编码区添加新序列，通过编码纤维蛋白的核苷酸序列的遗传工程来制备修饰的纤维蛋白。在优选的实施方案中，纤维蛋白质的N末端没有改变，尽管在一些实施方案中，可能需要抑制纤维蛋白质的细胞核的摄取，通过核靶向信号的缺失。修饰的纤维蛋白可用作用于基因治疗的重组腺病毒的一部分。

24. 发明授权

US06284742B1 Immunomodulation by genetic modification of dendritic cells and B cells 有权
标题翻译：通过树突状细胞和B细胞的遗传修饰免疫调节
公开(公告)号：US06284742B1
公开(公告)日：2001-09-04
申请号：US09407511
申请日：1999-09-28
申请人： David T. Curiel , Bryan Walter Tillman
发明人： David T. Curiel , Bryan Walter Tillman
IPC分类号： A61K4800
CPC分类号： C12N15/86 , A61K48/00 , A61K2039/505 , A61K2039/5154 , A61K2039/5156 , C07K16/081 , C07K16/2842 , C07K16/2878 , C07K2317/31 , C07K2317/55 , C07K2319/00 , C12N5/0635 , C12N5/0639 , C12N2501/22 , C12N2501/23 , C12N2510/00 , C12N2710/10343 , C12N2710/10345 , C12N2810/855 , C12N2810/859
摘要： A gene delivery vector used to generate genetically modified dendritic cells and B-cells central to the immune system. As a result, genetic modification of cells bearing the CD40 target antigen on its surface can be used to modulate immunity. Previously, both dendritic cells and B-cells have been resistant to gene transfer. The present invention serves to mediate dramatic enhancements in gene transfer to these cell types. Simultaneous with gene transfer, the vector system described herein matures dendritic cells and B-cells to a more potent immunoregulatory status. This invention provides technology for genetic manipulation of dendritic cells and B-cells.
摘要翻译：用于产生遗传修饰的树突状细胞和B细胞的免疫系统中枢的基因递送载体。结果，可以使用表面上具有CD40靶抗原的细胞的遗传修饰来调节免疫。以前，树突状细胞和B细胞都具有抗基因转移的能力。本发明用于介导对这些细胞类型的基因转移的显着增强。与基因转移同时，本文所述的载体系统使树突状细胞和B细胞成为更有效的免疫调节状态。本发明提供树突状细胞和B细胞的遗传操作技术。

25. 发明授权

US06274322B1 Composition for introducing nucleic acid complexes into higher eucaryotic cells 有权
标题翻译：用于将核酸复合物引入高级真核细胞的组合物
公开(公告)号：US06274322B1
公开(公告)日：2001-08-14
申请号：US09465646
申请日：1999-12-16
申请人： David T. Curiel , Max L. Birnstiel , Matthew Cotten , Ernst Wagner , Kurt Zatloukal , Christian Plank , Berndt Oberhauser , Walter G. M. Schmidt
发明人： David T. Curiel , Max L. Birnstiel , Matthew Cotten , Ernst Wagner , Kurt Zatloukal , Christian Plank , Berndt Oberhauser , Walter G. M. Schmidt
IPC分类号： C12Q168
CPC分类号： C12N15/87 , A61K38/00 , A61K47/50 , A61K47/6901 , A61K48/00 , C07K14/005 , C07K14/56 , C07K14/775 , C07K14/79 , C07K16/00 , C07K16/2803 , C07K2317/77 , C07K2319/00 , C12N7/00 , C12N2710/10251 , C12N2710/10261 , C12N2710/10322 , C12N2710/10351 , C12N2710/10361 , C12N2710/10363 , C12N2740/11022 , C12N2740/15022 , C12N2740/16022 , C12N2740/16122 , C12N2760/16122 , C12N2770/32722 , C12N2770/32761 , Y10T436/143333
摘要： A composition for the transfection of higher eucaryotic cells, comprising complexes of nucleic acid, a substance having an affinity for nucleic acid and optionally an internalizing factor, contains an endosomolytic agent, e.g. a virus or virus component, which may be conjugated. The endosomolytic agent, which is optionally part of the nucleic acid complex, is internalized into the cells together with the complex and releases the contents of the endosomes into the cytoplasm, thereby increasing the gene transfer capacity. Pharmaceutical preparations, transfection kits and methods for introducing nucleic acid into higher eucaryotic cells by treating the cells with the composition are also disclosed.
摘要翻译：用于转染高等真核细胞的组合物，其包含核酸复合物，对核酸和任选的内在因子具有亲和力的物质，含有内体细胞溶解剂可以结合的病毒或病毒组分。任选地是核酸复合物的一部分的内体细胞溶解剂与复合物一起被内化到细胞中，并将内体的内容物释放到细胞质中，从而增加基因转移能力。还公开了药物制剂，转染试剂盒和通过用组合物处理细胞将核酸引入高级真核细胞的方法。

26. 发明授权

US5910486A Methods for modulating protein function in cells using, intracellular antibody homologues 失效
标题翻译：使用细胞内抗体同系物调节细胞中蛋白质功能的方法
公开(公告)号：US5910486A
公开(公告)日：1999-06-08
申请号：US468252
申请日：1995-06-06
申请人： David T. Curiel , Jessy Deshane
发明人： David T. Curiel , Jessy Deshane
IPC分类号： A61K48/00 , C07K16/32
CPC分类号： C07K16/32 , A61K2039/505 , A61K48/00 , C07K2317/622 , C12N2799/022
摘要： Methods and compositions for modulating protein function in a cell involving intracellular expression of an antibody homologue that binds to the protein within the cell are disclosed. In a preferred embodiment, an antibody homologue, such as a single chain Fv (sFv) fragment, is expressed within an intracellular compartment of a cell, such as the endoplasmic reticulum (ER), to inhibit cell surface expression of a membrane protein. Preferably, the cell is a malignant mammalian cell and the protein is a cell surface receptor oncoprotein, such as c-erbB2. Intracellular binding of the antibody homologue to the receptor oncoprotein inhibits its surface expression and, moreover, inhibits cell proliferation and cell survival. Isolated nucleic acid molecules encoding anti-c-erbB2 antibody homologues, as well as recombinant expression vectors and host cells incorporating these nucleic acid molecules, are also disclosed.
摘要翻译：公开了涉及在细胞内与蛋白质结合的抗体同系物的细胞内表达的细胞中调节蛋白质功能的方法和组合物。在优选的实施方案中，抗体同系物，例如单链Fv（sFv）片段在细胞的细胞内隔室（例如内质网（ER））内表达，以抑制膜蛋白的细胞表面表达。优选地，细胞是恶性哺乳动物细胞，并且蛋白质是细胞表面受体癌蛋白，例如c-erbB2。抗体同源物与受体癌蛋白的细胞内结合抑制其表面表达，此外抑制细胞增殖和细胞存活。还公开了编码抗c-erbB2抗体同系物的分离的核酸分子，以及重组表达载体和掺入这些核酸分子的宿主细胞。

27. 发明授权

US06841540B1 Immunomodulation by genetic modification of dendritic cells and B cells 有权
标题翻译：通过树突状细胞和B细胞的遗传修饰免疫调节
公开(公告)号：US06841540B1
公开(公告)日：2005-01-11
申请号：US09591737
申请日：2000-06-12
申请人： David T. Curiel , Bryan Walter Tillman
发明人： David T. Curiel , Bryan Walter Tillman
IPC分类号： A61K48/00 , C07K16/08 , C07K16/28 , C12N5/0781 , C12N5/0784 , C12N15/861 , A61K21/70 , C07H21/02 , C12N5/06 , C12N15/74
CPC分类号： C12N15/86 , A61K48/00 , A61K2039/505 , A61K2039/5154 , A61K2039/5156 , C07K16/081 , C07K16/2842 , C07K16/2878 , C07K2317/31 , C07K2317/55 , C07K2319/00 , C12N5/0635 , C12N5/0639 , C12N2501/22 , C12N2501/23 , C12N2510/00 , C12N2710/10343 , C12N2710/10345 , C12N2810/855 , C12N2810/859
摘要： The present invention provides a CD40-targeted gene delivery system and a CD40-targeted recombinant adenoviral vector for genetic manipulation of dendritic cells and B cells. Also provided are methods of using this enhanced gene delivery to immune system cells and therefore, enhancing dendritic cell-based immunotherapy.
摘要翻译：本发明提供CD40靶向基因递送系统和用于遗传操作树突状细胞和B细胞的CD40靶向重组腺病毒载体。还提供了使用这种增强的基因递送到免疫系统细胞并因此增强基于树突状细胞的免疫治疗的方法。

28. 发明授权

US06699473B2 Human anti-epidermal growth factor receptor single-chain antibodies 失效
公开(公告)号：US06699473B2
公开(公告)日：2004-03-02
申请号：US09976118
申请日：2001-10-12
申请人： Kevin Paul Raisch , David T. Curiel , James Alan Bonner
发明人： Kevin Paul Raisch , David T. Curiel , James Alan Bonner
IPC分类号： C07K1628
CPC分类号： A61K51/103 , A61K2039/505 , C07K16/2863 , C07K2317/21 , C07K2317/622
摘要： Human anti-epidermal growth factor receptor (EGFR) single-chain antibodies (scFvs) were isolated from a human IgM phage display library using purified epidermal growth factor receptor as antigen. Two isolates with different amino acid sequences were identified by ELISA as epidermal growth factor receptor-specific. The scFvs bind to the full length epidermal growth factor receptor and the truncated and/or mutated epidermal growth factor receptor on human cells. These anti-EGFR-scFvs are useful as therapeutic and/or diagnostic agents.

29. 发明授权

US06683170B2 Ligands added to adenovirus fiber 失效
标题翻译：配体添加到腺病毒纤维中
公开(公告)号：US06683170B2
公开(公告)日：2004-01-27
申请号：US09904599
申请日：2001-07-13
申请人： David T. Curiel , Jeffrey A. Engler
发明人： David T. Curiel , Jeffrey A. Engler
IPC分类号： C07H2104
CPC分类号： C12N15/86 , A61K48/00 , C07K14/005 , C07K2319/00 , C12N15/87 , C12N2710/10322 , C12N2710/10343 , C12N2810/40
摘要： The fiber protein of adenovirus has been genetically altered via attachment at the carboxyl end of a peptide linker, preferably up to 26 amino acids in length which forms a random coil, which can be used to attach a non-adenovirus ligand altering the binding specificity of the fiber protein. Examples of ligands include peptides which are selectively bound by a targeted cell so that the modified fiber protein is internalized by receptor-mediated endocytosis, and peptides which can act as an universal coupling agent, for example, biotin or strepavidin. The linker is designed to not interfere with normal trimerization of fiber protein, to avoid steric hindrance of binding of the fiber protein to a targeted cell, and to serve as a site to introduce new peptide sequence.
摘要翻译：腺病毒的纤维蛋白已经通过在肽接头的羧基末端的连接被遗传改变，优选长达26个氨基酸长度，其形成无规卷曲，其可用于附着非腺病毒配体，其改变结合特异性纤维蛋白。配体的实例包括被靶细胞选择性结合以使经修饰的纤维蛋白质被受体介导的内吞作用内化的肽，以及可以作为通用偶联剂例如生物素或链霉亲和素的肽。接头被设计成不干扰纤维蛋白质的正常三聚，以避免纤维蛋白质与靶细胞结合的空间位阻，并用作引入新肽序列的位点。

30. 发明授权

US6077663A Composition for introducing nucleic acid complexes into higher eucaryotic cells 失效
标题翻译：用于将核酸复合物引入高级真核细胞的组合物
公开(公告)号：US6077663A
公开(公告)日：2000-06-20
申请号：US449754
申请日：1995-05-25
申请人： David T. Curiel , Max L. Birnstiel , Matthew Cotten , Ernst Wagner , Kurt Zatloukal , Christian Plank , Berndt Oberhauser , Walter G.M. Schmidt
发明人： David T. Curiel , Max L. Birnstiel , Matthew Cotten , Ernst Wagner , Kurt Zatloukal , Christian Plank , Berndt Oberhauser , Walter G.M. Schmidt
IPC分类号： C12N15/09 , A61K35/76 , A61K38/00 , A61K38/21 , A61K38/36 , A61K39/44 , A61K47/48 , A61K48/00 , A61P31/12 , A61P35/00 , C07K14/005 , C07K14/075 , C07K14/095 , C07K14/11 , C07K14/15 , C07K14/16 , C07K14/56 , C07K14/775 , C07K14/79 , C07K16/00 , C07K16/28 , C12N5/10 , C12N7/04 , C12N15/34 , C12N15/41 , C12N15/44 , C12N15/87 , G01N33/00 , C12Q1/68
CPC分类号： C12N15/87 , A61K47/48 , A61K47/48776 , A61K48/00 , C07K14/005 , C07K14/56 , C07K14/775 , C07K14/79 , C07K16/00 , C07K16/2803 , C12N7/00 , A61K38/00 , C07K2317/77 , C07K2319/00 , C12N2710/10251 , C12N2710/10261 , C12N2710/10322 , C12N2710/10351 , C12N2710/10361 , C12N2710/10363 , C12N2740/11022 , C12N2740/15022 , C12N2740/16022 , C12N2740/16122 , C12N2760/16122 , C12N2770/32722 , C12N2770/32761 , Y10T436/143333
摘要： A composition for the transfection of higher eucaryotic cells, comprising complexes of nucleic acid, a substance having an affinity for nucleic acid and optionally an internalizing factor, contains an endosomolytic agent, e.g. a virus or virus component, which may be conjugated. The endosomolytic agent, which is optionally part of the nucleic acid complex, is internalized into the cells together with the complex and releases the contents of the endosomes into the cytoplasm, thereby increasing the gene transfer capacity. Pharmaceutical preparations, transfection kits and methods for introducing nucleic acid into higher eucaryotic cells by treating the cells with the composition are also disclosed.
摘要翻译：用于转染高等真核细胞的组合物，其包含核酸复合物，对核酸和任选的内在因子具有亲和力的物质，含有内体细胞溶解剂可以结合的病毒或病毒组分。任选地是核酸复合物的一部分的内体细胞溶解剂与复合物一起被内化到细胞中，并将内体的内容物释放到细胞质中，从而增加基因转移能力。还公开了药物制剂，转染试剂盒和通过用组合物处理细胞将核酸引入高级真核细胞的方法。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式