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    • 27. 发明公开
    • MAIZE CYTOPLASMIC MALE STERILITY (CMS) S-TYPE RESTORER RF3 GENE, MOLECULAR MARKERS AND THEIR USE
    • S-TYP-WIEDERHERSTELLUNGSGEN RF3 DER CYTOPLASMISCHENMÄNNLICHENSTERILITÄT(CMS)VON MAIS,MOLEKULARE标志和DEREN VERWENDUNG
    • EP3089577A4
    • 2017-09-13
    • EP14877179
    • 2014-12-30
    • DOW AGROSCIENCES LLC
    • REN RUIHUANAGEL BRUCE AGIBSON RYANGAO YANXIN STARMAMMADOV JAFAR
    • A01H1/00
    • A01H1/04A01H1/02C07K14/415C12Q1/6895C12Q2600/13C12Q2600/156
    • The present disclosure provides a method for selecting a plant comprising a functional restorer gene for maize S-type cytoplasmic male sterility comprising the steps of (a) screening a population of plants for at least one marker nucleic acid, wherein the marker nucleic acid comprises an allele linked to the functional restorer gene for maize S-type cytoplasmic male sterility; (b) detecting the marker nucleic acid; (c) identifying a plant comprising the marker nucleic acid; and (d) selecting the plant comprising the marker nucleic acid, wherein the plant comprising the marker nucleic acid further comprises the functional restorer gene for maize S-type cytoplasmic male sterility. The present disclosure also provides methods for restoring fertility in a progeny of an S-type cytoplasmic male sterile plant and methods for transferring an Rf3 gene into a progeny plant.
    • 本公开提供了选择包含用于玉米S型细胞质雄性不育的功能性恢复基因的植物的方法,其包括以下步骤:(a)筛选植物群体中的至少一种标志物核酸,其中所述标志物核酸包含 等位基因与玉米S型细胞质雄性不育的功能恢复基因有关; (b)检测标志物核酸; (c)鉴定包含标记核酸的植物; 和(d)选择包含标记核酸的植物,其中包含标记核酸的植物还包含玉米S型细胞质雄性不育的功能恢复基因。 本公开内容还提供恢复S型细胞质雄性不育植物的后代中的可育性的方法和用于将Rf3基因转移至后代植物的方法。