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21. 发明授权

US10739245B2 Compact multi-color flow cytometer 有权
公开(公告)号：US10739245B2
公开(公告)日：2020-08-11
申请号：US15498397
申请日：2017-04-26
申请人： CYTEK BIOSCIENCES, INC.
发明人： David Vrane , Ming Yan , Eric Chase , Wenbin Jiang
IPC分类号： G01N15/14 , G01N21/64 , G01N15/10 , G01N15/00
摘要： A system, an apparatus, and a method are provided for a modular flow cytometer with a compact size. In one embodiment, the modular flow cytometry system includes the following: a laser system for emitting laser beams; a flow cell assembly positioned to receive the laser beams at an interrogation region of a fluidics stream where fluoresced cells scatter the laser beams into fluorescent light; a fiber assembly positioned to collect the fluorescent light; and a grating system including a dispersive element and a receiver assembly, wherein the dispersive element is positioned to receive the fluorescent light from the fiber assembly and to direct spectrally dispersed light toward the receiver assembly.

22. 发明申请

US20200049614A1 SMART FLOW CYTOMETERS WITH SELF MONITORING AND SELF VALIDATION 审中-公开
公开(公告)号：US20200049614A1
公开(公告)日：2020-02-13
申请号：US16101385
申请日：2018-08-10
申请人： CYTEK BIOSCIENCES, INC.
发明人： Janine Jiang , David Vrane
IPC分类号： G01N15/14
摘要： In some embodiments, a smart flow cytometer includes a monitoring system to monitor differing operational parameters of the smart flow cytometer to detect an advanced failure of components and an advanced need for maintenance. In others, a smart flow cytometer includes a quality control system including a reservoir of quality control beads to periodically run a validation test with the quality control beads in order to determine the ability of the flow cytometer to generate quality data output from a plurality of light detectors. In some embodiments, a plurality of smart flow cytometers are coupled into communication with a computer communication network; a central repair server system is coupled into communication with the computer communication network and the plurality of smart flow cytometers; wherein each of the plurality of smart flow cytometers includes a monitoring system coupled to monitor differing operational parameters of the smart flow cytometer for possible failure.

23. 发明申请

US20180156710A1 FLOW CYTOMETERY SYSTEM WITH FLUIDICS CONTROL SYSTEM 审中-公开
公开(公告)号：US20180156710A1
公开(公告)日：2018-06-07
申请号：US15817237
申请日：2017-11-19
申请人： CYTEK BIOSCIENCES, INC.
发明人： David Vrane
IPC分类号： G01N15/14
CPC分类号： G01N15/1404 , G01N15/1434 , G01N15/1436 , G01N15/147 , G01N21/49 , G01N2015/1006 , G01N2015/1075 , G01N2015/1409 , G01N2015/1438
摘要： A system, method, and apparatus are provided for flow cytometry. In one example, the flow cytometry system includes dual laser devices and dual scatter channels to measure velocity of particles in a core stream of sample fluid. The total flow rate of the sample fluid and the sheath fluid around the sample fluid is controlled, and thus held constant, by a feedback control system controlling a vacuum pump based on differential pressure across ends of a flow channel in the flow cell.

24. 发明授权

US11965813B2 Methods and apparatus for central source pressure-based cytometer fluidics system 有权
公开(公告)号：US11965813B2
公开(公告)日：2024-04-23
申请号：US17390984
申请日：2021-08-01
申请人： CYTEK BIOSCIENCES, INC.
发明人： David Vrane
IPC分类号： G01N15/14 , G01N15/1404 , G01N15/10
CPC分类号： G01N15/1404 , G01N2015/1006 , G01N2015/1409
摘要： A system, method, and apparatus are provided for cytometer fluidics. In one example, a pressure regulation system is provided for a cytometer fluidics system. The pressure regulation system includes a pressure regulator and a transducer. The pressure regulator pressurizes sheath fluid in a fluid path. The transducer senses a measured pressure in the fluid path independently of the sheath tank. The transducer converts the measured pressure into a voltage. The transducer communicates the voltage to the pressure regulator. The pressure regulator translates the voltage to a regulated pressure that substantially matches the measured pressure. The pressure regulator is the only pressure regulation source in the cytometry fluidics system.

25. 发明授权

US11913868B2 Compact detection module for flow cytometers 有权
公开(公告)号：US11913868B2
公开(公告)日：2024-02-27
申请号：US17302651
申请日：2021-05-10
申请人： CYTEK BIOSCIENCES, INC.
发明人： Ming Yan , Yung-Chieh Hsieh , David Vrane , Eric Chase
IPC分类号： G01N15/00 , G01J3/00 , G01J1/08 , G01N21/47 , G01N15/14 , G01J3/02 , G01J3/36 , G01N15/10 , G01J3/12
CPC分类号： G01N15/1436 , G01J1/08 , G01J3/021 , G01J3/0208 , G01J3/0218 , G01J3/0256 , G01J3/0291 , G01J3/36 , G01N15/1434 , G01N15/1459 , G01N21/47 , G01J2003/1213 , G01N2015/1006 , G01N2015/145
摘要： In one embodiment, a flow cytometer is disclosed having a compact light detection module. The compact light detection module includes an image array with a transparent block, a plurality of micro-mirrors in a row coupled to a first side of the transparent block, and a plurality of filters in a row coupled to a second side of the transparent block opposite the first side. Each of the plurality of filters reflects light to one of the plurality of micro-mirrors and passes light of a differing wavelength range and each of the plurality of micro-mirrors reflects light to one of the plurality of filters, such that incident light into the image array zigzags back and forth between consecutive filters of the plurality of filters and consecutive micro-mirrors of the plurality of micro-mirrors. A radius of curvature of each of the plurality of micro-mirrors images the fiber aperture onto the odd filters and collimates the light beam on the even filters.

26. 发明授权

US11879826B2 Flow cytometer communication system for smart flow cytometers with self monitoring 有权
公开(公告)号：US11879826B2
公开(公告)日：2024-01-23
申请号：US17412695
申请日：2021-08-26
申请人： Cytek Biosciences, Inc.
发明人： Janine Jiang , David Vrane
IPC分类号： G01N15/00 , G01N15/14
CPC分类号： G01N15/1431 , G01N15/1425 , G01N15/1434 , G01N2015/1438
摘要： In some embodiments, a plurality of smart flow cytometers are coupled into communication with a computer communication network. A central repair server system is coupled into communication with the computer communication network and the plurality of smart flow cytometers. Each of the plurality of smart flow cytometers includes a monitoring system coupled to monitor differing operational parameters of the smart flow cytometer for possible failure. The monitoring system can detect an advanced failure of components based on the operational parameters being monitored. The monitoring system can also detect an advanced need for repair and maintenance based on the operational parameters being monitored.

27. 发明申请

US20220371017A1 CELL SORTER WITH PIVOTAL SCUPPERS 有权
公开(公告)号：US20220371017A1
公开(公告)日：2022-11-24
申请号：US17829321
申请日：2022-05-31
申请人： CYTEK BIOSCIENCES, INC.
发明人： Glen Krueger , David Vrane , Kuncheng Wang , Qiuta Gu
IPC分类号： B01L3/00 , G01N15/14
摘要： A compact sorting flow cytometer system is disclosed. The system includes a fluidics system having a flow cell and a deflection chamber in communication with the flow cell to receive drops in a stream of a sample biological fluid with one or more biological cells or particles and selectively deflect the drops in the stream of the sample biological fluid with the one or more biological cells or particles; and a droplet deposition unit (DDU) system in communication with the deflection chamber to receive the selectively deflected drops in the stream of the sample biological fluid with the one or more biological cells or particles into one or more containers. The DDU system includes a case or a housing with an open face surround by edges of the case, the case forming a portion of a containment chamber, the case having a top side opening aligned with the deflection chamber to receive the selectively deflected drops in the stream of the sample biological fluid into one or more containers in the containment chamber, a seal mounted around edges of the case, one or more hinges coupled to a bottom portion of the case, and a door coupled to the one or more hinges to pivot the door about the one or more hinges, the door when closed to press against the seal and close off the containment chamber from an external environment.
A method for evacuation of air in a containment chamber of a flow cytometer is disclosed. The method includes turning off a return fan in a first tunnel between an air conditioning chamber and a containment chamber; turning on an evacuation fan in a second tunnel between the air conditioning chamber and the containment chamber, the evacuation fan pulling air out of the containment chamber into the air conditioning chamber, opening a valve in an evacuation vent, the evacuation fan pushing air out of the air conditioning chamber through the evacuation vent into the environment; and continuously running the evacuation fan for a predetermined period of time to evacuate air out of the containment chamber.

28. 发明申请

US20220268688A1 INTEGRATED COMPACT CELL SORTER 有权
公开(公告)号：US20220268688A1
公开(公告)日：2022-08-25
申请号：US17665480
申请日：2022-02-04
申请人： CYTEK BIOSCIENCES, INC.
发明人： Glenn Krueger , David Vrane
IPC分类号： G01N15/14 , C12M1/00
摘要： A flow cytometry or cell sorting system includes a fluidics system and a flow cell. Under pressure, the fluidics system causes sheath and sample biological fluids to flow. The fluidics system can include a gas bubble to remove and eliminate gas bubbles in the sheath fluid; The flow cell communicates with the fluidics system to receive the sheath fluid, wherein a sample biological fluid flows with cells or particles through the flow cell to be surrounded by the sheath fluid; a deflection chamber under the flow cell to receive the drops of sample biological fluid and sheath fluid out of the flow cell, the deflection chamber to selectively deflect one or more of the drops along one or more deflection paths; and a droplet deposition unit (DDU) system in communication with the deflection chamber to receive selectively deflected drops in the stream of the sample biological fluid with the one or more biological cells or particles into one or more containers.
A flow cytometer or cell sorter system includes a fluidics system and a flow cell. The fluidics system is under pressure to cause a sheath fluid and a sample fluid to flow, the fluidics system including a gas bubble remover eliminating gas bubbles in the sheath fluid; a flow cell coupled in communication with the fluidics system to receive the sheath fluid, wherein a sample fluid flows with cells or particles through the flow cell to be surrounded by the sheath fluid. The flow cell includes a drop drive assembly, a flow cell body, and a cuvette coupled together. The drop drive assembly includes a sample injection tube (SIT) in communication with the fluidics system to receive sample fluid. The flow cell body receives the sample fluid from the sample injection tube and sheath fluid. The flow cell body has a charging port to charge the droplets, the flow cell body having a funnel portion to form a fluid stream of the sample fluid surrounded by the sheath fluid out of an opening; and a cuvette coupled to a base of the flow cell body, the cuvette having a channel to receive the fluid stream of the sample fluid surrounded by the sheath fluid out of the opening, the cuvette being transparent to light and allowing the sample fluid to undergo interrogation in the channel by a plurality of different lasers to determine a plurality of different types of cells or particles therein.
A flow cell body for a flow cytometer or a cell sorter is provided. The flow cell body comprises the following: a three-dimensional opaque (e.g., black) polymer body having top, bottom, left, right, front, and back sides. The opaque polymer body includes the following: a top side opening into a chamber to receive a drop drive assembly including a sample injection tube (SIT), wherein the chamber has an upper circular cylindrical portion and a lower funnel portion; two or more opposing top side openings receiving threaded inserts to engage two or more threaded bolts to hold a hub of the drop drive assembly coupled to the top side of the flow cell body; a left side port opening into the chamber adjacent a top of the lower funnel portion of the chamber, the left side port opening for fluid flow into or out of the chamber; a right side port opening into the chamber adjacent the top of the lower funnel portion of the chamber, the right side port opening for fluid flow out of or into the chamber; a bottom side opening into the chamber to allow a fluid stream to exit the chamber and the flow cell body; and wherein the lower funnel portion of the chamber forms the fluid stream out of the bottom side opening.
A subsystem for a flow cytometer or cell sorter system is provided. The subsystem comprises the following: a carriage assembly including a mount having a through hole, wherein the mount is formed to register a nozzle assembly having a nozzle insert with a center nozzle orifice, wherein a gasket is coupled around a perimeter of the nozzle insert, and wherein the mount registers the nozzle assembly such that the center nozzle orifice and the through hole of the mount are concentric along a center axis; the carriage assembly further including a carriage plate statically coupled to the mount such that the carriage plate enables the mount to have vertical movement along the center axis, wherein the mount presses the gasket on the nozzle assembly against a lower side of a cuvette, and wherein the cuvette is formed to have a lower side facing the mount and an upper side coupled to a base of a flow cell body.
A flow cytometer or cell sorter system is provided. The system comprises the following: a flow cell coupled in communication with the fluidics system to receive the sheath fluid, wherein a sample fluid flows with cells or particles through the flow cell to be surrounded by the sheath fluid, the flow cell including a flow cell body coupled around the drop drive assembly to receive the sample fluid from the sample injection tube, the flow cell body coupled in communication with the fluidics system to receive the sheath fluid, the flow cell body having charging port to charge the droplets, the flow cell body having a funnel portion to form a fluid stream of the sample fluid surrounded by the sheath fluid out of an opening; a cuvette coupled to a base of the flow cell body, the cuvette having a channel to receive the fluid stream of the sample fluid surrounded by the sheath fluid out of the opening, the cuvette being transparent to light and allowing the sample fluid to undergo interrogation in the channel by a plurality of different lasers to determine a plurality of different types of cells or particles therein; and a nozzle assembly selectively engaged with the cuvette, the nozzle assembly having a nozzle and an O-ring around the nozzle selectively pressed against a face of the cuvette around the channel, the nozzle receiving the sample stream from the cuvette and forming sample drops out of the nozzle assembly.
A nozzle assembly for a cell sorter system is provided. The nozzle assembly comprises the following: a nozzle handle having a body with a gripping end and a nozzle end, the body having a through hole between top and bottom surfaces near the nozzle end with a partial gland in the top surface extending around the through hole, the partial gland having a slot extending out from the through hole to the nozzle end of the nozzle handle; a nozzle insert positioned in a portion of the through hole of the body of the nozzle handle, the nozzle insert having a circular body with a center nozzle orifice concentric with the through hole to flow drops of a sample fluid, and a beveled ring in a top surface extending out from the circular body; a gasket positioned in the partial gland against the beveled ring of the nozzle insert with a portion extending above the top surface of the nozzle insert and the top surface of the nozzle handle, the gasket to provide a seal around the center nozzle orifice; and wherein the slot extending out from the partial gland to the nozzle end facilitates removal of the gasket.
A flow cytometer or cell sorter system is provided. The system comprises the following: a fluidics system under pressure to cause a sheath fluid and a sample fluid to flow, the fluidics system including a gas bubble remover eliminating gas bubbles in the sheath fluid; and a flow cell coupled in communication with the fluidics system to receive the sheath fluid, wherein a sample fluid flows with cells or particles through the flow cell to be surrounded by the sheath fluid. The flow cell includes the following: a flow cell body coupled in communication with the fluidics system to receive the sheath fluid, the flow cell body having charging port to charge the droplets, the flow cell body having a chamber with a circular cylindrical portion and a funnel portion, the funnel portion to form a fluid stream of the sample fluid surrounded by the sheath fluid out of a bottom side opening; a drop drive assembly coupled to the flow cell body, the drop drive assembly including a glass sample injection tube (SIT) inserted into the chamber of the flow cell body and having a first end located in the funnel portion of the chamber, the glass sample injection tube having a second end coupled in communication with the fluidics system to receive the sample fluid and inject the sample fluid into the funnel portion of the chamber; and a cuvette coupled to a base of the flow cell body, the cuvette having a flow channel adjacent the bottom side opening of the flow cell body, the cuvette to receive the fluid stream of the sample fluid surrounded by the sheath fluid out of the bottom side opening, the cuvette being transparent to light and allowing the sample fluid to undergo interrogation in the flow channel by a plurality of different lasers to determine a plurality of different types of cells or particles in the sample fluid.
A drop drive assembly for a flow cytometer or cell sorter system is provided. The drop drive assembly comprises the following: an outer metal (piezo) hub having a center opening extending from top to bottom through an extended hollow circular plug; an insulating spacer extended over the extended hollow circular plug up to a flange in a base of the outer metal hub; a hollow piezoelectric cylindrical transducer having a first end coupled over the extended hollow circular plug up to insulating spacer, an inner terminal of the hollow piezoelectric cylindrical transducer coupled to the extended hollow circular plug to form an electrical connection to the outer metal hub; an insulated cylindrical sealing base having an extended hollow circular plug coupled into a second end of the hollow piezoelectric cylindrical transducer, the insulated cylindrical sealing base having a gland ring around a through hole opposite the extended hollow circular plug, the insulated cylindrical sealing base having a groove around an outer cylindrical surface; a hollow cylindrical glass sample injection tube (SIT) having a first end inserted into and through the center opening in the outer metal (piezo) hub, the hollow piezoelectric cylindrical transducer, and the through hole in the insulated cylindrical sealing base; a first sealing O-ring mounted in the groove around in the outer cylindrical surface of the insulated cylindrical sealing base, the first sealing O-ring to engage a cylindrical wall of a cylindrical chamber to seal fluids away from the hollow piezoelectric cylindrical transducer; and a second sealing O-ring mounted in the gland ring in the insulated cylindrical sealing base around the hollow cylindrical glass sample injection tube (SIT) to seal fluids away from the hollow piezoelectric cylindrical transducer.

29. 发明申请

US20220252501A1 MAGNETICALLY COUPLED COLLECTING SYSTEM FOR FLOW CYTOMETRY AND CELL SORTER SYSTEMS 有权
公开(公告)号：US20220252501A1
公开(公告)日：2022-08-11
申请号：US17665489
申请日：2022-02-04
申请人： CYTEK BIOSCIENCES, INC.
发明人： Babak Honaryar , David Vrane , Peter Troung , Kuncheng Wang , Max Schwartz
IPC分类号： G01N15/14 , G01D5/347 , G01D5/14 , G01D5/24
摘要： A collecting system with a magnetically coupled sample mover is provided for flow cytometry and cell sorter systems. The collecting system uses magnets in a driver carriage to control the position of other magnets in a follower carriage. The driver carriage can thereby control the position of the follower carriage without physically touching the follower carriage.

30. 发明授权

US11169076B2 Compact detection module for flow cytometers 有权
公开(公告)号：US11169076B2
公开(公告)日：2021-11-09
申请号：US15659610
申请日：2017-07-25
申请人： CYTEK BIOSCIENCES, INC.
发明人： Ming Yan , Yung-Chieh Hsieh , David Vrane , Eric Chase
IPC分类号： G01N15/00 , G01N21/00 , G01J3/00 , G01N15/14 , G01J1/08 , G01J3/02 , G01J3/36 , G01N21/47 , G01J3/12 , G01N15/10
摘要： In one embodiment, a flow cytometer is disclosed having a compact light detection module. The compact light detection module includes an image array with a transparent block, a plurality of micro-mirrors in a row coupled to a first side of the transparent block, and a plurality of filters in a row coupled to a second side of the transparent block opposite the first side. Each of the plurality of filters reflects light to one of the plurality of micro-mirrors and passes light of a differing wavelength range and each of the plurality of micro-mirrors reflects light to one of the plurality of filters, such that incident light into the image array zigzags back and forth between consecutive filters of the plurality of filters and consecutive micro-mirrors of the plurality of micro-mirrors. A radius of curvature of each of the plurality of micro-mirrors images the fiber aperture onto the odd filters and collimates the light beam on the even filters.

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