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    • 22. 发明公开
    • 대장균 유래 ptsL 프로모터를 함유하는 재조합벡터 및이를 이용한 외래 단백질의 제조방법
    • 包含PTSL促进剂的重组载体和使用其生产异构蛋白的方法
    • KR1020090018315A
    • 2009-02-20
    • KR1020070082686
    • 2007-08-17
    • 한국과학기술원
    • 이상엽박태정정순
    • C12N15/66C12N15/09C12N15/10
    • A recombinant vector containing ptsL promoter derived from Escherichia coli is provided to easily obtain the foreign protein after the stationary growth phase without the cell-crushing process by inducing cytolysis through expression of protein harmful to bacteria or antimicrobial peptide within the cell. The recombinant vector containing ptsL promoter derived from Escherichia coli which contains DNA encoding ptsL promoter activator having the nucleotide sequence of SEQ ID NO:1 and a gene encoding the foreign protein, and optionally a gene encoding the expression regulatory protein of the ptsL gene and the promoter for expressing the expression regulatory protein, wherein the gene encoding expression regulatory protein of ptsL gene is mlc encoding the expression-inhibiting protein of ptsL gene, having the nucleotide sequence of SEQ ID NO:2.
    • 提供含有源自大肠杆菌的ptsL启动子的重组载体,通过在细胞内表达对细菌或抗微生物肽有害的蛋白质进行细胞分解,在静止生长期后容易地获得外源蛋白,无需细胞破碎过程。 含有源自大肠杆菌的ptsL启动子的重组载体含有编码具有SEQ ID NO:1的核苷酸序列的ptsL启动子激活剂的DNA和编码外来蛋白质的基因,以及任选地编码ptsL基因表达调节蛋白的基因和 用于表达调控蛋白的启动子,其中编码ptsL基因的表达调节蛋白的基因是编码具有SEQ ID NO:2的核苷酸序列的ptsL基因的表达抑制蛋白的mlc。
    • 27. 发明公开
    • 초음파 융착기용 지그 어셈블리
    • 超声波焊接机用超声波焊接方法及其超声波焊接方法
    • KR1020130104292A
    • 2013-09-25
    • KR1020120025674
    • 2012-03-13
    • 한국과학기술원
    • 이태재이석재박태정이경균김병일
    • B23K20/10B29C65/08B23K20/26
    • PURPOSE: A jig assembly for a transonic welding machine and a melting method using the same are provided to accurately process materials with reducing time for melting overlapped materials. CONSTITUTION: A jig assembly for a transonic welding machine comprises a base (10), a fixing jig (20), and a driving jig (30). The base is for maintaining horizontality. The fixing jig is fixed to the base, and comprises a concave surface formed into a semicircular shape. The driving jig comprises a table with a clamp for fixing materials. The driving jig comprises a block section corresponding to the concave surface in order to improve the degree of freedom. The driving jig comprises an adsorption ball in the circumferential direction in order to fix an angle of the driving jig.
    • 目的:提供用于跨音速焊接机的夹具组件和使用该夹具的熔融方法,以在缩短重叠材料熔融时间的情况下精确地处理材料。 构成:用于跨音速焊接机的夹具组件包括基座(10),固定夹具(20)和驱动夹具(30)。 基础是保持水平。 固定夹具固定在基座上,包括形成半圆形的凹面。 驱动夹具包括具有用于固定材料的夹具的工作台。 驱动夹具包括与凹面对应的块部,以提高自由度。 驱动夹具包括沿圆周方向的吸附球,以固定驱动夹具的角度。
    • 29. 发明公开
    • 구리 결합펩타이드를 이용한 탐침단백질이 고정된 다중 스팟 구리 증착형 나노구조체 배열 바이오칩 및 이의 제조방법
    • 使用铜结合肽的多点铜复合纳米生物培养基及其制备方法
    • KR1020120033875A
    • 2012-04-09
    • KR1020100095612
    • 2010-09-30
    • 한국과학기술원
    • 이상엽박태정김도균
    • G01N33/68G01N33/52G01N21/84
    • PURPOSE: A multi-spot copper-capped nanostructure array biochip using copper binding peptides is provided to detect interaction of DNA and various bio substances at high sensitivity. CONSTITUTION: A method for manufacturing a multi-spot copper-capped nanostructure array biochip using CBP(copper binding peptide) comprises: a step of forming a metal thin film(second layer) on a substrate(first layer); a step of forming a multi-spot on the metal thin film and arranging the nanostructure in predetermined interval to form nanosturcutre arranged layer(third layer); a step of forming copper thin film(fourth layer) on the nanosturcutre arranged layer; and a step of fixing CBP-probe protein-conjugated fusion proteins on the copper thin film. The substrate includes glass, polystyrene, PET(polyethylene terephthalate), polycarbonate, silicon, or quartz.
    • 目的:提供使用铜结合肽的多点铜帽纳米结构阵列生物芯片,以检测DNA和各种生物物质在高灵敏度下的相互作用。 构成:使用CBP(铜结合肽)制造多点铜封端纳米结构阵列生物芯片的方法包括:在衬底(第一层)上形成金属薄膜(第二层)的步骤; 在金属薄膜上形成多点并以预定间隔布置纳米结构以形成纳米级刻蚀层(第三层)的步骤; 在纳米薄壁布置层上形成铜薄膜(第四层)的步骤; 以及将CBP-探针蛋白质结合的融合蛋白固定在铜薄膜上的步骤。 基板包括玻璃,聚苯乙烯,PET(聚对苯二甲酸乙二醇酯),聚碳酸酯,硅或石英。
    • 30. 发明公开
    • 형광 중공 실리카 나노입자 및 이의 제조방법
    • 荧光二氧化硅纳米微粒及其制备方法
    • KR1020120005749A
    • 2012-01-17
    • KR1020100066381
    • 2010-07-09
    • 한국과학기술원
    • 김도현이경균위린복이석재박태정
    • C09K11/02A61K9/51
    • PURPOSE: Fluorescent hollow silica nanoparticles and a producing method thereof are provided to stably mass produce a core unit with a big hollow hole inside the nanoparticles. CONSTITUTION: Fluorescent hollow silica nanoparticles comprise the following: a core unit with a hollow hole having the diameter of 1-100nm; a silica shell unit surrounding the core unit; and fluorescent materials contained in the silica shell unit. A producing method of the fluorescent hollow silica nanoparticles comprises the following steps: applying the fluorescent materials to a synthesis process of silica nanoparticle; and processing the obtained fluorescent silica nanoparticles with hydrofluoric acid for producing pores inside the nanoparticles.
    • 目的:提供荧光中空二氧化硅纳米颗粒及其制备方法,以稳定地大量生产纳米颗粒内部具有大空心孔的芯单元。 构成:荧光中空二氧化硅纳米颗粒包括以下:具有中空孔的核心单元,其直径为1-100nm; 围绕所述核心单元的二氧化硅壳单元; 和包含在二氧化硅壳单元中的荧光材料。 荧光中空二氧化硅纳米颗粒的制备方法包括以下步骤:将荧光物质施加到二氧化硅纳米颗粒的合成过程中; 并用氢氟酸处理得到的荧光二氧化硅纳米粒子,以在纳米粒子内产生孔。