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21. 发明申请

US20110160087A1 METHOD FOR INCREASING EFFICIENCY OF GERMPLASM SCREENING IN PLANT TRANSFORMATION 失效
标题翻译：增加植物转化筛选效率的方法
公开(公告)号：US20110160087A1
公开(公告)日：2011-06-30
申请号：US12649673
申请日：2009-12-30
申请人： ZUO-YU ZHAO , XINLI E. WU , MYOENG-JE CHO , DEPING XU
发明人： ZUO-YU ZHAO , XINLI E. WU , MYOENG-JE CHO , DEPING XU
IPC分类号： C40B30/06
CPC分类号： C12N15/8209 , A01H1/04 , C12N15/8201 , C12N15/8205
摘要： A method for increasing efficiency of germplasm screening for transformability may include providing a plurality of lines of plant target tissue to be transformed, characterizing each of the lines to provide characterization data, the characterization data comprises DNA or nucleic acid delivery technique response data and tissue culture response data, eliminating one or more of the plurality of lines based on the characterization data without performing transformation of the plurality of lines, such that a subset of the plurality of lines remains, and performing transformation experiments on the subset of the plurality of lines. The method may also include selecting a DNA or nucleic acid delivery technique protocol and a tissue culture protocol prior to the characterization.
摘要翻译：提高种质筛选效率的方法可包括提供要转化的多条植物靶组织，表征各条线以提供特征数据，表征数据包括DNA或核酸递送技术应答数据和组织培养响应数据，基于所述表征数据消除所述多行中的一个或多个，而不执行所述多条线的变换，使得保留所述多条线的子集，并对所述多条线的所述子集执行变换实验。该方法还可以包括在表征之前选择DNA或核酸递送技术方案和组织培养方案。

22. 发明申请

US20090300789A1 Highly Transformable Elite Inbred Line-PHWWE 有权
标题翻译：高度可变的精英自交系 - PHWWE
公开(公告)号：US20090300789A1
公开(公告)日：2009-12-03
申请号：US12534507
申请日：2009-08-03
申请人： Ning Wang , Lynn L. Heetland , William E. Solawetz , Zuo-Yu Zhao
发明人： Ning Wang , Lynn L. Heetland , William E. Solawetz , Zuo-Yu Zhao
IPC分类号： A01H5/00 , A01H1/02 , C12N5/04 , C12N15/82
CPC分类号： A01H5/10
摘要： A novel inbred maize line designated PHWWE and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing inbred maize line PHWWE with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PHWWE through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the inbred line PHWWE or a trait conversion of PHWWE with another maize line. Inbred maize lines derived from maize line PHWWE, methods for producing other inbred maize lines derived from inbred maize line PHWWE and the inbred maize lines and their parts derived by the use of those methods.
摘要翻译：一种新型近交玉米品系被指定为PHWWE及其种子，植物和植物部分。用于生产玉米植物的方法，其包括用另一玉米植物杂交近交玉米品系PHWWE。用于生产含有其遗传物质的玉米植物的方法通过回交转化和/或转化渗透到PHWWE中的一种或多种性状，以及由此产生的玉米种子，植物和植物部分的方法。杂交玉米种子，植物或植物部分通过交叉自交系PHWWE或PHWWE与另一种玉米品系的转化而产生。衍生自玉米系PHWWE的近交玉米品系，由近交玉米系PHWWE生产其他近交玉米品系的方法以及通过使用这些方法得到的近交玉米品系及其成分。

23. 发明申请

US20090293141A1 Highly Transformable Elite Doubled Haploid Line-PHWWD 有权
标题翻译：高度可变的精英双倍倍数单倍体PHWWD
公开(公告)号：US20090293141A1
公开(公告)日：2009-11-26
申请号：US12534487
申请日：2009-08-03
申请人： Ning Wang , Michael D. Miller , Lynn L. Heetland , William E. Solawetz , Zuo-Yu Zhao
发明人： Ning Wang , Michael D. Miller , Lynn L. Heetland , William E. Solawetz , Zuo-Yu Zhao
IPC分类号： A01H1/00 , A01H5/10 , A01H5/00 , C12N5/04
CPC分类号： A01H5/10
摘要： A novel double haploid maize line designated PHWWD and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing double haploid maize line PHWWD with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PHWWD through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the double haploid line PHWWD or a trait conversion of PHWWD with another maize line. Inbred maize lines derived from double haploid maize line PHWWD, methods for producing other inbred maize lines derived from double haploid maize line PHWWD and the inbred maize lines and their parts derived by the use of those methods.
摘要翻译：一种新颖的双倍体单倍体玉米系命名为PHWWD及其种子，植物和植物部分。用于生产玉米植物的方法，其包括用另一种玉米植物杂交双倍体单倍体玉米品系PHWWD。用于生产含有其遗传物质的玉米植物的方法通过回交转化和/或转化渗透到PHWWD中的一种或多种性状，以及由此生产的玉米种子，植物和植物部分。杂交玉米种子，植物或植物部分通过双重单倍体系PHWWD或PHWWD与另一个玉米品系的转化而产生。来自双倍体单倍体玉米品系PHWWD的近交玉米品系，由双倍体单倍体玉米品系PHWWD生产其他近交玉米品系的方法，以及通过使用这些方法得到的近交玉米品系及其成分。

24. 发明申请

US20080216198A1 Doubled haploid cells, embryos and plants 审中-公开
标题翻译：双倍体单倍体细胞，胚胎和植物
公开(公告)号：US20080216198A1
公开(公告)日：2008-09-04
申请号：US12054556
申请日：2008-03-25
申请人： Zuo-Yu Zhao , Dennis L. Bidney , Evan D. Elsing , Michael D. Miller , Xinli E. Wu , William J. Gordon-Kamm
发明人： Zuo-Yu Zhao , Dennis L. Bidney , Evan D. Elsing , Michael D. Miller , Xinli E. Wu , William J. Gordon-Kamm
IPC分类号： C12N15/29 , C12N15/11
CPC分类号： C12N15/8241 , A01H5/10 , C12N15/8201 , C12N15/829
摘要： Methods for producing homozygous plants, seeds, and plant cells are provided. Also provided are methods of transformation.
摘要翻译：提供了用于产生纯合植物，种子和植物细胞的方法。还提供了转化的方法。

25. 发明申请

US20080216191A1 Doubling of Chromosomes in Haploid Embryos 有权
标题翻译：染色体倍倍数在单倍体胚胎
公开(公告)号：US20080216191A1
公开(公告)日：2008-09-04
申请号：US11532921
申请日：2006-09-19
申请人： Joanne E. Barton , Sheila E. Maddock , Xinli E. Wu , Zuo-Yu Zhao , Mark E. Williams , Tanveer Hussain , William J. Gordon-Kamm
发明人： Joanne E. Barton , Sheila E. Maddock , Xinli E. Wu , Zuo-Yu Zhao , Mark E. Williams , Tanveer Hussain , William J. Gordon-Kamm
IPC分类号： A01H1/08 , A01H5/00
CPC分类号： A01H1/08
摘要： Methods for producing doubled haploid corn (Zea mays) plants, seeds, and plant cells are provided.
摘要翻译：提供了生产双倍单倍体玉米（Zea mays）植物，种子和植物细胞的方法。

26. 发明授权

US06822144B1 Methods for Agrobacterium-mediated transformation 失效
标题翻译：农杆菌介导转化方法
公开(公告)号：US06822144B1
公开(公告)日：2004-11-23
申请号：US08963096
申请日：1997-11-03
申请人： Zuo-Yu Zhao , Weining Gu , Tishu Cai , Dorothy A. Pierce
发明人： Zuo-Yu Zhao , Weining Gu , Tishu Cai , Dorothy A. Pierce
IPC分类号： C12N1584
CPC分类号： C12N15/8205
摘要： The present invention relates to methods for improving the transformation frequency of Agrobacterium-mediated transformation of maize embryos. A preferred method for transforming maize using Agrobacterium comprises the steps of: contacting at least one immature embryo from a maize plant with Agrobacterium capable of transferring at least one gene to said embryo; co-cultivating the embryos with Agrobacterium; culturing the embryos in medium comprising N6 salts, an antibiotic capable of inhibiting the growth of Agrobacterium, and a selective agent to select for embryos expressing the gene; and regenerating plants expressing the gene.
摘要翻译：本发明涉及改善农杆菌介导的玉米胚转化的转化频率的方法。使用农杆菌转化玉米的优选方法包括以下步骤：使来自玉米植物的至少一种未成熟胚与能够将至少一种基因转移至所述胚的农杆菌接触; 与土壤杆菌共培养胚胎; 在包含N6盐的培养基中培养胚胎，能够抑制农杆菌生长的抗生素，以及选择表达基因的胚胎的选择剂; 和再生表达该基因的植物。

27. 发明授权

US08859846B2 Doubling of chromosomes in haploid embryos 有权
标题翻译：染色体倍增在单倍体胚胎
公开(公告)号：US08859846B2
公开(公告)日：2014-10-14
申请号：US11532921
申请日：2006-09-19
申请人： Joanne E. Barton , Sheila E. Maddock , Xinli E. Wu , Zuo-Yu Zhao , Mark E. Williams , Tanveer Hussain , William J. Gordon-Kamm
发明人： Joanne E. Barton , Sheila E. Maddock , Xinli E. Wu , Zuo-Yu Zhao , Mark E. Williams , Tanveer Hussain , William J. Gordon-Kamm
IPC分类号： A01H1/08 , A01H4/00
CPC分类号： A01H1/08
摘要： Methods for producing doubled haploid corn (Zea mays) plants, seeds, and plant cells are provided.
摘要翻译：提供了生产双倍单倍体玉米（Zea mays）植物，种子和植物细胞的方法。

28. 发明授权

US08404930B2 Methods for improving monocot transformation 失效
标题翻译：改善单子叶植物转化的方法
公开(公告)号：US08404930B2
公开(公告)日：2013-03-26
申请号：US12694013
申请日：2010-01-26
申请人： Xinli E. Wu , Myeong-Je Cho , Zuo-Yu Zhao
发明人： Xinli E. Wu , Myeong-Je Cho , Zuo-Yu Zhao
IPC分类号： C12N15/84 , A01H4/00
CPC分类号： C12N15/8205
摘要： The invention provides improved plant transformation methods. In particular the method provides increased transformation frequency, especially in recalcitrant plants. The method includes various transformation protocols for monocots, such as maize and sorghum, using a combination of media and light conditions to achieve increased efficiency of monocot transformation and increased callus initiation frequencies.
摘要翻译：本发明提供改进的植物转化方法。特别地，该方法提供增加的转化频率，特别是在顽固植物中。该方法包括用于单子叶植物（例如玉米和高粱）的各种转化方案，其使用培养基和光照条件的组合来实现提高单子叶植物转化的效率和增加的愈伤组织起始频率。

29. 发明授权

US08039719B2 Highly transformable elite doubled haploid line-PHWWD 有权
标题翻译：高度可变的精英双倍体单倍体PHWWD
公开(公告)号：US08039719B2
公开(公告)日：2011-10-18
申请号：US12534487
申请日：2009-08-03
申请人： Ning Wang , Michael D. Miller , Lynn L. Heetland , William E. Solawetz , Zuo-Yu Zhao
发明人： Ning Wang , Michael D. Miller , Lynn L. Heetland , William E. Solawetz , Zuo-Yu Zhao
IPC分类号： A01H5/00 , A01H5/10 , A01H1/02 , C12N5/04
CPC分类号： A01H5/10
摘要： A novel double haploid maize line designated PHWWD and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing double haploid maize line PHWWD with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PHWWD through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the double haploid line PHWWD or a trait conversion of PHWWD with another maize line. Inbred maize lines derived from double haploid maize line PHWWD, methods for producing other inbred maize lines derived from double haploid maize line PHWWD and the inbred maize lines and their parts derived by the use of those methods.
摘要翻译：一种新颖的双倍体单倍体玉米系命名为PHWWD及其种子，植物和植物部分。用于生产玉米植物的方法，其包括用另一种玉米植物杂交双倍体单倍体玉米品系PHWWD。用于生产含有其遗传物质的玉米植物的方法通过回交转化和/或转化渗透到PHWWD中的一种或多种性状，以及由此生产的玉米种子，植物和植物部分。杂交玉米种子，植物或植物部分通过双重单倍体系PHWWD或PHWWD与另一个玉米品系的转化而产生。来自双倍体单倍体玉米品系PHWWD的近交玉米品系，由双倍体单倍体玉米品系PHWWD生产其他近交玉米品系的方法，以及通过使用这些方法得到的近交玉米品系及其成分。

30. 发明申请

US20090293157A1 PRODUCTION OF PROGENITOR CEREAL CELLS 审中-公开
标题翻译：生产谷胱甘肽原
公开(公告)号：US20090293157A1
公开(公告)日：2009-11-26
申请号：US12358158
申请日：2009-01-22
申请人： Luke Mehlo , Zuo-Yu Zhao
发明人： Luke Mehlo , Zuo-Yu Zhao
IPC分类号： A01H5/00 , C12N5/04 , C12N5/10 , C12Q1/68
CPC分类号： C12N15/8205 , A01H4/005 , A01H4/008
摘要： A process for the production and maintenance of pluripotent and/or totipotent progenitor cereal cells from undifferentiated callus cells is described. Production of the progenitor cells takes place via direct organogenesis on a medium containing at least one auxin and at least one cytokinin. For example, the auxin may be 2,4-dichlorophenoxyacetic acid, indole-3-acetic acid, picloran, naphthelenacetic acid, indole-3-propionic acid, indole-3-butyric acid, phenyl acetic acid, benzofuran-3-acetic acid or phenyl butyric acid, and the cytokinin may be benzyl amino purine, benzyladenine, thidiazuron, zeatin, isopentyladenine, trans-zeatin or dimethylallyladenine. Processes for transformation of the undifferentiated callus cells and/or the progenitor cereal cells are also described. Typical cereal cells are sorghum, maize, wheat, barley, millet, rye, canola, alfalfa, triticale and rice.
摘要翻译：描述了用于从未分化愈伤组织细胞产生和维持多能和/或全能祖先谷物细胞的方法。在含有至少一种生长素和至少一种细胞分裂素的培养基上通过直接器官发生进行祖细胞的产生。例如，生长素可以是2,4-二氯苯氧基乙酸，吲哚-3-乙酸，吡咯兰，萘扁
乙酸，吲哚-3-丙酸，吲哚-3-丁酸，苯乙酸，苯并呋喃-3-乙酸或苯基丁酸，细胞分裂素可以是苄基氨基嘌呤，苄基腺嘌呤，噻替隆，玉米素，异戊二醛，反式玉米素或二甲基烯丙基。还描述了用于转化未分化愈伤组织细胞和/或祖细胞谷粒细胞的方法。典型的谷类细胞是高粱，玉米，小麦，大麦，小米，黑麦，卡诺拉，苜蓿，黑小麦和水稻。

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