专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

21. 发明授权

US5019270A Perfusive chromatography 失效
标题翻译：渗透色谱法
公开(公告)号：US5019270A
公开(公告)日：1991-05-28
申请号：US595661
申请日：1990-10-09
申请人： Noubar B. Afeyan , Fred E. Regnier , Robert C. Dean, Jr.
发明人： Noubar B. Afeyan , Fred E. Regnier , Robert C. Dean, Jr.
IPC分类号： B01D15/08 , B01D15/34 , G01N30/52
CPC分类号： B01J20/282 , B01D15/345 , B01J20/28095 , B01J2220/54 , G01N2030/528
摘要： Disclosed are chromatography methods and matrix geometries which permit high resolution, high productivity separation of mixtures of solutes, particularly biological materials. The method involves passing fluids through specially designed chromatography matrices at high flow rates. The matrices define first and second interconnected sets of pores and a high surface area for solute interaction in fluid communication with the members of the second set of pores. The first and second sets of pores are embodied, for example, as the interstices among particles and throughpores within the particles. The pores are dimensioned such that, at achievable high fluid flow rates, convective flow occurs in both pore sets, and the convective flow rate exceeds the rate of solute diffusion in the second pore set. This approach couples convective and diffusive mass transport to and from the active surface and permits increases in fluid velocity without the normally expected bandspreading.
摘要翻译：公开了色谱方法和基质几何形状，其允许溶质，特别是生物材料的混合物的高分辨率，高生产率分离。该方法包括以高流速将流体通过专门设计的色谱基质。这些基质定义了第一和第二相互连接的孔组，以及用于与第二组孔的构件流体连通的溶质相互作用的高表面积。第一和第二组孔体现为例如颗粒内的颗粒和透孔之间的间隙。孔的尺寸使得在可实现的高流体流速下，在两个孔组中都发生对流，并且对流流速超过第二孔组中溶质扩散的速率。这种方法将对流和扩散质量传输与活性表面相耦合，并允许流体速度的增加而没有通常预期的带扩展。

IPRDB

热门服务

关于我们

友情链接

联系方式