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21. 发明专利

CA6752A BUGGY SPRING COUPLING AND SUPPORT 未知
公开(公告)号：CA6752A
公开(公告)日：1876-11-09
申请号：CA6752D
申请人： MCBRIDE JOHN
发明人： MCBRIDE JOHN

22. 发明申请

WO2010124000A2 MODIFIED CIPA GENE FROM CLOSTRIDIUM THERMOCELLUM FOR ENHANCED GENETIC STABILITY 审中-公开
标题翻译：用于增强热稳定性的改性CIPA基因用于增强的遗传稳定性
公开(公告)号：WO2010124000A2
公开(公告)日：2010-10-28
申请号：PCT/US2010031907
申请日：2010-04-21
申请人： DARTMOUTH COLLEGE , CURRIE DEVIN HEDLEY , MCBRIDE JOHN , GUSS ADAM
发明人： CURRIE DEVIN HEDLEY , MCBRIDE JOHN , GUSS ADAM
IPC分类号： C12N15/31 , C12N15/74 , C12R1/145
CPC分类号： C12N9/2434 , C12P19/20 , Y02E50/16
摘要： Bacteria consume a variety of biomass-derived substrates and produce ethanol. The scaffoldin gene cipA from Clostridium thermocellum is modified to generate a mutated gene with enhanced genetic stability. This mutated cipA gene can be introduced into a heterologous host, such as Thermoanaerobacterium saccharolyticum. Other cellulosome components may be introduced into the host to build a full-sized cellulosome in T. saccharolyticum. Manipulation of the scaffoldin genes provides a new approach for enhancing ethanol production by biomass-fermenting microorganisms.
摘要翻译：细菌消耗各种生物质衍生的底物并产生乙醇。来自热链霉菌的支架蛋白基因cipA被修饰以产生具有增强的遗传稳定性的突变基因。该突变的cipA基因可以被引入异源宿主，例如解糖酵母杆菌（Thermoanerobacterium saccharoticticum）。可以将其它纤维素酶组分引入宿主以在解糖酵母中构建全尺寸的纤维素体。脚手架基因的操作为生物质发酵微生物提高乙醇生产提供了新的途径。

23. 发明申请

WO2010060056A2 YEAST EXPRESSING CELLULASES FOR SIMULTANEOUS SACCHARIFICATION AND FERMENTATION USING CELLULOSE 审中-公开
标题翻译： YEAST表达细胞同时发酵和发酵使用纤维素
公开(公告)号：WO2010060056A2
公开(公告)日：2010-05-27
申请号：PCT/US2009/065571
申请日：2009-11-23
申请人： MASCOMA CORPORATION , MCBRIDE, John , BREVNOVA, Elena , GHANDI, Chhayal , MELLON, Mark , FROEHLICH, Alan , DELEAULT, Kristen , RAJGHARIA, Vineet , FLATT, Jim , VAN ZYL, Emile , DEN HAAN, Riaan , LAGRANGE, Danie , ROSE, Shaunita , PENTTILA, Merja , ILMEN, Marja , SIIKA-AHO, Matti , UUSITALO, Jaana , HAU, Heidi Hanson , RICE, Charles , VILLARI, Jeff , STONEHOUSE, Emily A. , GILBERT, Alan , KEATING, Jeffrey D. , XU, Haowen , WILLES, Deidre , SHIKHARE, Indraneel , THORNGREN, Naomi , WARNER, Anne K. , MURPHY, Dan
发明人： MCBRIDE, John , BREVNOVA, Elena , GHANDI, Chhayal , MELLON, Mark , FROEHLICH, Alan , DELEAULT, Kristen , RAJGHARIA, Vineet , FLATT, Jim , VAN ZYL, Emile , DEN HAAN, Riaan , LAGRANGE, Danie , ROSE, Shaunita , PENTTILA, Merja , ILMEN, Marja , SIIKA-AHO, Matti , UUSITALO, Jaana , HAU, Heidi Hanson , RICE, Charles , VILLARI, Jeff , STONEHOUSE, Emily A. , GILBERT, Alan , KEATING, Jeffrey D. , XU, Haowen , WILLES, Deidre , SHIKHARE, Indraneel , THORNGREN, Naomi , WARNER, Anne K. , MURPHY, Dan
IPC分类号： C12P7/06
CPC分类号： C12P7/10 , C12N9/2437 , C12N9/2445 , C12N15/80 , C12P7/14 , C12P39/00 , C12Y302/01004 , C12Y302/01021 , C12Y302/01091 , Y02E50/16 , Y02E50/17
摘要： The present invention is directed to cellulytic host cells. The host cells of the invention expressing heterologous cellulases and are able to produce ethanol from cellulose. According to the invention, host cells expressing a combination of heterologous cellulases can be used to produce ethanol from cellulose. In addition, multiple host cells expressing different heterlogous cellulases can be co-cultured together and used to produce ethanol from cellulose. Furthemore, the invention demonstrates for the first time the ability of Kluveryomyces to produce ethanol from cellulose. The yeast strains and co-cultures of yeast strains of the invention can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of saccharification and fermentation processes.
摘要翻译：本发明涉及纤维素分解宿主细胞。表达异源纤维素酶的本发明的宿主细胞能够从纤维素中产生乙醇。根据本发明，表达异源纤维素酶组合的宿主细胞可用于从纤维素生产乙醇。此外，表达不同杂种纤维素酶的多个宿主细胞可以共同共培养并用于从纤维素生产乙醇。此外，本发明首次证明了Kluveryomyces从纤维素中生产乙醇的能力。本发明的酵母菌株的酵母菌株和共培养物可以独立地用于生产乙醇，也可以与外部添加的纤维素酶组合使用，以提高糖化和发酵过程的效率。

24. 发明申请

WO2005116271A2 SELECTION OF MICROORGANISMS WITH GROWTH DEPENDENT UPON EXTRACYTOPLASMIC ENZYMES 审中-公开
标题翻译：选择与增殖相关的微生物在提取物中的增殖
公开(公告)号：WO2005116271A2
公开(公告)日：2005-12-08
申请号：PCT/US2005/018430
申请日：2005-05-25
申请人： THE TRUSTEES OF DARTMOUTH COLLEGE , FAN, Zhilian , MCBRIDE, John , LYND, Lee, R.
发明人： FAN, Zhilian , MCBRIDE, John , LYND, Lee, R.
IPC分类号： C12R1/00
CPC分类号： C12N15/01 , C12N1/36
摘要： A method for selection of microorganisms with improved growth on non-native substrates is disclosed. Strains expressing tethered, enzymes on their cell surface are grown on non-native substrates and mutants with improved growth characteristics relative to the parent strain can be advantageously selected. Both soluble and insoluble substrates may be employed.
摘要翻译：公开了一种在非天然基质上选择具有改善生长的微生物的方法。在非天然底物上生长表达系统的酶，其细胞表面的酶的菌株可以有利地选择具有相对于亲本菌株具有改善的生长特性的突变体。可以使用可溶性和不溶性底物。

25. 发明申请

WO1994005026A1 IMAGING SYSTEM 审中-公开
标题翻译：成像系统
公开(公告)号：WO1994005026A1
公开(公告)日：1994-03-03
申请号：PCT/GB1993001784
申请日：1993-08-20
申请人： UNIVERSITY OF SOUTHAMPTON , McBRIDE, John, William , WEAVER, Paul, Michael
发明人： UNIVERSITY OF SOUTHAMPTON
IPC分类号： H01H11/00
CPC分类号： H01H11/0062
摘要： An imaging system permits the study of motion of the electric arc formed during the breaking operation of a miniature circuit breaker under short circuit conditions. The imaging system employs an array of up to 45 optical fibres to detect the light emitted by the arc in the arc chamber of the miniature circuit breaker. The light from the fibres is detected electronically and stored digitally then transferred to a computer. Over 4000 images can be captured at a rate of 1 image per (my)s. A computer program uses the optical information to display an image of the arc on the screen. The imaging process includes steps of: (a) identifying a group of event locations at which the event parameter sample value exceeds a predetermined threshold value; (b) identifying event locations at the boundary of an area encompassing the group; (c) responding to the identified boundary event locations to plot a line representing a contour of constant event parameter value corresponding to the threshold value; and (d) increasing the predetermined threshold and repeating steps (a), (b) and (c) for the increased threshold until a desired number of contours lines for respective event parameter threshold values have been generated.
摘要翻译：成像系统允许研究在短路条件下微型断路器的断开操作期间形成的电弧的运动。成像系统采用多达45根光纤的阵列来检测由微型断路器的电弧室中的电弧发出的光。来自光纤的光被电子地检测并数字地存储然后传送到计算机。可以以每个（我）的1个图像的速度捕获超过4000张图像。计算机程序使用光学信息在屏幕上显示弧的图像。成像处理包括以下步骤：（a）识别事件参数采样值超过预定阈值的一组事件位置; （b）识别包围该组的区域边界处的事件位置; （c）响应所识别的边界事件位置来绘制表示对应于该阈值的常数事件参数值的轮廓的线; 和（d）增加预定阈值，并且对于增加的阈值重复步骤（a），（b）和（c），直到已经产生用于各个事件参数阈值的期望数量的轮廓线为止。

26. 发明申请

WO2011153516A3 YEAST EXPRESSING SACCHAROLYTIC ENZYMES FOR CONSOLIDATED BIOPROCESSING USING STARCH AND CELLULOSE 审中-公开
标题翻译：使用淀粉和纤维素的YEAST表达用于综合生物处理的芳香酶
公开(公告)号：WO2011153516A3
公开(公告)日：2012-03-29
申请号：PCT/US2011039192
申请日：2011-06-03
申请人： MASCOMA CORP , BREVNOVA ELENA , MCBRIDE JOHN E , WISWALL ERIN , WENGER KEVIN S , CAIAZZA NICKY , HAU HEIDI H , ARGYROS AARON , AGBOGBO FRANK , RICE CHARLES F , BARRETT TRISHA , BARDSLEY JOHN S , FOSTER ABIGAIL S , WARNER ANNE K , MELLON MARK , SKINNER RYAN , SHIKHARE INDRANEEL , DEN HAAN RIAAN , GANDHI CHHAYAL V , BELCHER ALAN , RAJGARHIA VINEET B , FROEHLICH ALLAN C , DELEAULT KRISTEN M , STONEHOUSE EMILY , TRIPATHI SHITAL A , GOSSELIN JENNIFER , CHIU YIN-YING , XU HAOWEN , UNIV STELLENBOSCH
发明人： BREVNOVA ELENA , MCBRIDE JOHN E , WISWALL ERIN , WENGER KEVIN S , CAIAZZA NICKY , HAU HEIDI H , ARGYROS AARON , AGBOGBO FRANK , RICE CHARLES F , BARRETT TRISHA , BARDSLEY JOHN S , FOSTER ABIGAIL S , WARNER ANNE K , MELLON MARK , SKINNER RYAN , SHIKHARE INDRANEEL , DEN HAAN RIAAN , GANDHI CHHAYAL V , BELCHER ALAN , RAJGARHIA VINEET B , FROEHLICH ALLAN C , DELEAULT KRISTEN M , STONEHOUSE EMILY , TRIPATHI SHITAL A , GOSSELIN JENNIFER , CHIU YIN-YING , XU HAOWEN
IPC分类号： C12N1/15 , C12N1/19
CPC分类号： C12N15/52 , C12N9/2405 , C12N9/2437 , C12N9/2445 , C12N9/248 , C12N9/2482 , C12N15/81 , C12P7/06 , C12Y302/01004 , C12Y302/01008 , C12Y302/01021 , C12Y302/01091 , Y02E50/17
摘要： The present invention is directed to a. yeast strain, or strains, secreting a full suite, or any subset of that, full suite, of enzymes to hydrolyze com starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicelhiiose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured togetherand used to produce ethanol from biomass feedstock.
摘要翻译：本发明涉及a。酵母菌株或菌株，分泌全套或全套酶的任何子集，以水解com淀粉，玉米纤维，木质纤维素（包括水解纤维素，半纤维素和木质素和碳水化合物之间的连接的酶）和利用戊糖（木糖和阿拉伯糖）。本发明还涉及在酵母中对于各种酶活性良好表达的蛋白质组。所得菌株或菌株可以同时水解淀粉和纤维素。所产生的菌株或菌株也可以代谢工程化以产生较少的甘油和吸收乙酸盐。所得的菌株或菌株也可用于从颗粒淀粉生产乙醇而不液化。所得到的菌株或菌株可以进一步用于减少在同时糖化和发酵（SSF）过程中水解生物质原料所需的外部酶的量，或者在当前蔗糖分解酶负载下在SSF期间提高乙醇的产率。此外，本发明的多种酶可以在本发明的细胞中共表达以提供对生物质原料的协同消化作用。在一些方面，表达不同异源糖分解酶的宿主细胞也可以共同共同培养并用于从生物质原料生产乙醇。

27. 发明申请

WO2011140386A2 DETOXIFICATION OF BIOMASS DERIVED ACETATE VIA METABOLIC CONVERSION TO ETHANOL, ACETONE, ISOPROPANOL, OR ETHYL ACETATE 审中-公开
标题翻译：通过代谢转化成乙醇，乙腈，异丙醇或乙酸乙酯的生物质衍生的乙酸脱氧
公开(公告)号：WO2011140386A2
公开(公告)日：2011-11-10
申请号：PCT/US2011/035416
申请日：2011-05-05
申请人： MASCOMA CORPORATION , SILLERS, William Ryan , VAN DIJKEN, Hans , LICHT, Steve , SHAW IV, Arthur J. , GILBERT, Alan Benjamin , ARGYROS, Aaron , FROEHLICH, Allan C. , MCBRIDE, John E. , XU, Haowen , HOGSETT, David A. , RAJGARHIA, Vineet B.
发明人： SILLERS, William Ryan , VAN DIJKEN, Hans , LICHT, Steve , SHAW IV, Arthur J. , GILBERT, Alan Benjamin , ARGYROS, Aaron , FROEHLICH, Allan C. , MCBRIDE, John E. , XU, Haowen , HOGSETT, David A. , RAJGARHIA, Vineet B.
IPC分类号： C12N1/18
CPC分类号： C12N15/81 , C12N1/16 , C12N1/18 , C12N9/0006 , C12N9/0008 , C12N9/0067 , C12N15/74 , C12P7/02 , C12P7/04 , C12P7/06 , C12P7/10 , C12P7/28 , C12P7/62 , C12R1/01 , C12R1/645 , C12R1/865 , C12Y102/01012 , C12Y112/99006 , Y02E50/16 , Y02E50/17 , Y02P20/52
摘要： One aspect of the invention relates to a genetically modified thermophilic or mesophilic microorganism, wherein a first native gene is partially, substantially, or completely deleted, silenced, inactivated, or down-regulated, which first native gene encodes a first native enzyme involved in the metabolic production of an organic acid or a salt thereof, thereby increasing the native ability of said thermophilic or mesophilic microorganism to produce lactate or acetate as a fermentation product. In certain embodiments, the aforementioned microorganism further comprises a first non-native gene, which first non-native gene encodes a first non-native enzyme involved in the metabolic production of lactate or acetate. Another aspect of the invention relates to a process for converting lignocellulosic biomass to lactate or acetate, comprising contacting lignocellulosic biomass with a genetically modified thermophilic or mesophilic microorganism.
摘要翻译：本发明的一个方面涉及经遗传修饰的嗜热或嗜温微生物，其中第一天然基因部分，基本上或完全缺失，沉默，失活或下调，所述第一天然基因编码参与代谢产生有机酸或其盐，从而增加所述嗜热或嗜温微生物产生作为发酵产物的乳酸盐或乙酸盐的天然能力。在某些实施方案中，上述微生物还包含第一非天然基因，其第一非天然基因编码参与乳酸盐或乙酸盐的代谢产生的第一非天然酶。本发明的另一方面涉及将木质纤维素生物质转化为乳酸盐或乙酸盐的方法，包括使木质纤维素生物质与遗传修饰的嗜热或嗜温微生物接触。

28. 发明申请

WO2010096562A3 YEAST CELLS EXPRESSING AN EXOGENOUS CELLULOSOME AND METHODS OF USING THE SAME 审中-公开
标题翻译：表达外源纤维素酶的酵母细胞及其使用方法
公开(公告)号：WO2010096562A3
公开(公告)日：2010-10-14
申请号：PCT/US2010024592
申请日：2010-02-18
申请人： MASCOMA CORP , MCBRIDE JOHN , MELLON MARK , RAJGHARIA VINEET , BREVNOVA ELENA E , WISWALL ERIN , HOGSETT DAVID A , LAGRANGE DANIE , ROSE SHAUNITA , VAN ZYL EMILE
发明人： MCBRIDE JOHN , MELLON MARK , RAJGHARIA VINEET , BREVNOVA ELENA E , WISWALL ERIN , HOGSETT DAVID A , LAGRANGE DANIE , ROSE SHAUNITA , VAN ZYL EMILE
IPC分类号： C12P7/10 , C07K14/33 , C12N9/42 , C12N15/56 , C12N15/62
CPC分类号： C12P7/10 , C07K14/33 , C07K2319/035 , C07K2319/70 , C12N9/2402 , C12N9/2437 , C12N9/2445 , C12N9/248 , C12N15/81 , C12Y302/01004 , C12Y302/01008 , C12Y302/01021 , C12Y302/01037 , C12Y302/01091 , Y02E50/16 , Y02P20/52
摘要： The present invention relates to the engineering and expression of heterologous cellulosomes in microorganisms in order to facilitate the conversion of biomass to useful products. In some embodiments, the invention relates to the expression of scaffoldin proteins which form the nucleus of a cellulosome. Cellulases or other biomass-degrading enzymes can be non-covalently linked to the scaffoldin protein by virtue of a dockerin domain-cohesin domain interaction.
摘要翻译：本发明涉及在微生物中工程化和表达异源纤维素体以促进生物质向有用产物的转化。在一些实施方案中，本发明涉及形成细胞体的细胞核的支架蛋白的表达。凭借dockerin结构域 - 粘连素结构域相互作用，纤维素酶或其他生物质降解酶可以非共价连接到支架蛋白。

29. 发明申请

WO2010075529A2 HETEROLOGOUS BIOMASS DEGRADING ENZYME EXPRESSION IN THERMOANAEROBACTERIUM SACCHAROLYTICUM 审中-公开
标题翻译：热酵母菌中异源生物量降解酶的表达
公开(公告)号：WO2010075529A2
公开(公告)日：2010-07-01
申请号：PCT/US2009/069443
申请日：2009-12-23
申请人： MASCOMA CORPORATION , HAU, Heidi , RICE, Charles , HERRING, Chris , MCBRIDE, John , SHAW, IV, Arthur J. , WISWALL, Erin
发明人： HAU, Heidi , RICE, Charles , HERRING, Chris , MCBRIDE, John , SHAW, IV, Arthur J. , WISWALL, Erin
IPC分类号： C12P7/06
CPC分类号： C12N15/74 , C12N9/2437 , C12P7/065 , C12P7/10 , Y02E50/16 , Y02E50/17
摘要： The present invention is directed to thermophilic gram-positive anaerobic host cells. The thermophilic gram-positive anaerobic host cells of the invention, for example Themoanaerobacterium sachharolyticum (" T. sacch "), express heterologous biomass degrading enzymes, such as cellulases, and are able to produce useful fermentation products from cellulose. Useful fermentation products include, for example, ethanol, acetic acid, lactic acid or CO 2 . In order to provide maximum expression and activity levels, biomass degrading enzymes can be expressed from codon-optimized nucleotide sequences, can be expressed under the control of a high-efficiency promoter, and/or can be fused to a signal peptide. In addition, the host cell, for example, a T. sacch host cell, can be genetically altered to further improve ethanol production, for example by disrupting the production of organic products other than ethanol.
摘要翻译：本发明涉及嗜热革兰氏阳性厌氧宿主细胞。本发明的嗜热革兰氏阳性厌氧宿主细胞，例如莎草精酵母（“茶糖酵母”）表达异源生物降解酶，例如纤维素酶，并且能够从纤维素产生有用的发酵产物。有用的发酵产物包括例如乙醇，乙酸，乳酸或二氧化碳。为了提供最大的表达和活性水平，生物降解酶可以由密码子优化的核苷酸序列表达，可以在高效启动子的控制下表达，和/或可以与信号肽融合。此外，宿主细胞，例如，可以遗传改变宿主细胞，以进一步改善乙醇生产，例如通过破坏乙醇以外的有机产物的生产。

30. 发明申请

WO2010005551A3 HETEROLOGOUS EXPRESSION OF TERMITE CELLULASES IN YEAST 审中-公开
标题翻译：酵母细胞在异育银鲫中的异源表达
公开(公告)号：WO2010005551A3
公开(公告)日：2010-03-18
申请号：PCT/US2009003970
申请日：2009-07-07
申请人： MASCOMA CORP , BREVNOVA ELENA E , RAJGARHIA VINEET , MELLON MARK , WARNER ANNE , MCBRIDE JOHN , GANDHI CHHAYAL , WISWALL ERIN
发明人： BREVNOVA ELENA E , RAJGARHIA VINEET , MELLON MARK , WARNER ANNE , MCBRIDE JOHN , GANDHI CHHAYAL , WISWALL ERIN
IPC分类号： C07K14/435 , C12N1/16 , C12N9/42 , C12N15/81
CPC分类号： C12N9/2437 , C12N15/81 , C12N15/815 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01091 , Y02E50/16
摘要： The present invention provides for heterologous expression of termite and termite- associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast Saccharomyces cerevisiae. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.
摘要翻译：本发明提供了白蚁和白蚁相关共生体纤维素酶的异源表达。例如，纤维素酶可以是密码子优化的并在酵母宿主细胞如酵母酿酒酵母中表达。纤维素酶也可以与其他纤维素酶在宿主细胞中共表达。在这种宿主细胞中表达白蚁和白蚁相关共生体纤维素酶及其变体和组合的表达导致具有改善的纤维素活性的酵母。因此，这样的基因和表达系统对于有效且成本有效的整合生物处理系统是有用的。

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