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    • 27. 发明申请
    • TWO-COLOR NANOPORE SEQUENCING
    • 双色纳米孔测序
    • WO2017209891A1
    • 2017-12-07
    • PCT/US2017/031129
    • 2017-05-04
    • QUANTAPORE, INC.
    • GUEGLER, KarlSIMONS, Jan F.MACEVICZ, Stephen C.
    • C12Q1/06C12Q1/68G01N21/62G01N33/48G01N33/483
    • The invention is directed to methods for analyzing polymers comprising linear chains of at least two types of monomers, such as polynucleotides, including DNA, RNA, and the like, using nanopores and optical detection. In some embodiments, as few as two different kinds of nucleotide are labeled with different optical labels that generate distinguishable optical signals for the selected kinds of nucleotide in both sense strands and antisense strands of target polynucleotides. Labeled strands are translocated through nanopores where nucleotides of the strands are constrained to pass sequentially through an optical detection region where their labels generate a sequence of optical signals making up an optical signature. In some embodiments, information from optical signatures from both sense and antisense strands are combined to determine complete nucleotide sequences of target polynucleotides.
    • 本发明涉及使用纳米孔和光学检测来分析聚合物的方法,所述聚合物包含至少两种类型的单体(诸如多核苷酸,包括DNA,RNA等)的线性链。 在一些实施方案中,少至两种不同类型的核苷酸用不同的光学标记物标记,所述不同的光学标记物针对靶多核苷酸的有义链和反义链中的选定种类的核苷酸产生可区分的光学信号。 标记的链通过纳米孔易位,其中链的核苷酸被限制顺序地通过光学检测区域,其中它们的标记产生构成光学标记的一系列光学信号。 在一些实施方案中,来自有义和反义链的光学特征的信息被组合以确定靶多核苷酸的完整核苷酸序列。