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11. 发明公开

KR1020030082708A 블루엔소마이신 생합성 효소의 유전자 염기서열 및 아미노산 서열과 그 형질전환체 无效
标题翻译：蓝霉素生物合成酶基因序列和氨基酸序列及其转化体系
公开(公告)号：KR1020030082708A
公开(公告)日：2003-10-23
申请号：KR1020020021164
申请日：2002-04-18
申请人： 주식회사 바이오홀딩스 , 서주원
发明人： 서주원 , 양영열 , 현창구 , 정용균
IPC分类号： C12N15/52
CPC分类号： C12N9/1241 , C12N9/88 , C12N15/70 , C12Y207/07024 , C12Y402/01046
摘要： PURPOSE: Gene sequence and amino acid sequence of bluensomycin biosynthesis enzyme and a transformant transformed therewith are provided. The bluensomycin biosynthesis enzyme has improved antibiotic synthesis effect. CONSTITUTION: A gene encoding dTDP-glucose synthase (BlmD) isolated from Streptomyces bluensis (ATCC 27420) has the nucleotide sequence of SEQ ID NO: 1 and the amino acid sequence of SEQ ID NO: 2. A gene encoding dTDP-glucose 4,6-dehydratase (BlmE) isolated from Streptomyces bluensis (ATCC 27420) has the nucleotide sequence of SEQ ID NO: 3 and the amino acid sequence of SEQ ID NO: 4. A recombinant cosmid vector pHCG 584 contains the genes of SEQ ID NOs: 1 and 2. A transformant is produced by transforming E. coli BL21 with pETBlmD which is prepared by inserting blmD isolated from the cosmid vector pHCG 584 to pET21a+. A transformant is produced by transforming E. coli BL21 with pETBlmE which is prepared by inserting blmE isolated from the cosmid vector pHCG 584 to pET21a+.
摘要翻译：目的：提供蓝霉素生物合成酶的基因序列和氨基酸序列及其转化的转化体。蓝霉素生物合成酶具有改善的抗生素合成效果。构成：从Streptomyces bluensis（ATCC 27420）分离的编码dTDP-葡萄糖合成酶（BlmD）的基因具有SEQ ID NO：1的核苷酸序列和SEQ ID NO：2的氨基酸序列。编码dTDP-葡萄糖4的基因，从Streptomyces bluensis（ATCC 27420）分离的6-脱水酶（BlmE）具有SEQ ID NO：3的核苷酸序列和SEQ ID NO：4的氨基酸序列。重组粘粒载体pHCG 584含有SEQ ID NO：通过用通过将从粘粒载体pHCG 584分离的blmD插入pET21a +制备的pETBlmD转化大肠杆菌BL21产生转化体。通过用pETBlmE转化大肠杆菌BL21产生转化体，其通过将从粘粒载体pHCG 584分离的blmE插入pET21a +而制备。

12. 发明公开

KR1020020089927A ｄＴＤＰ-글루코즈 4,6-디하이드라타제 유전자, 재조합벡터, 형질전환체 및 이를 이용한 내열성ｄＴＤＰ-글루코즈 4,6-디하이드라타제의 제조 방법 失效
标题翻译： DTDP-葡萄糖4,6-脱水酶基因，含有该基因的重组载体，其转化体及使用该转化体生产耐热DTDP-葡萄糖4,6-脱水酶的方法
公开(公告)号：KR1020020089927A
公开(公告)日：2002-11-30
申请号：KR1020010028973
申请日：2001-05-25
申请人： 주식회사 진켐
发明人： 우진석 , 송재경 , 심성보 , 이희찬 , 류광경 , 강선엽 , 이주호 , 김대희
IPC分类号： C12N15/53
CPC分类号： C12N9/88 , C12N15/70 , C12Y402/01046
摘要： PURPOSE: Provided are a dTDP-glucose 4,6-dehydratase gene, a recombinant vector containing the same gene, a transformant therewith and a method for producing a heat-resistant dTDP-glucose 4,6-dehydratase using the same transformant. Therefore, the heat-resistant enzyme can be useful in medical industry. CONSTITUTION: The dTDP-glucose 4,6-dehydratase gene having the nucleotide sequence of SEQ ID NO: 1 is derived from Thermus caldophilus GK24. A recombinant vector pTHE10 contains the dTDP-glucose 4,6-dehydratase gene of SEQ ID NO: 1. A transformant E. coli TDPDH(KCTC 0999BP) is produced by transforming with the vector pTHE10. The method for producing the heat-resistant dTDP-glucose 4,6-dehydratase comprises steps of: (1) inserting the dTDP-glucose 4,6-dehydratase gene of SEQ ID NO: 1 into an expression vector to prepare the recombinant vector pTHE10; transforming Escherichia coli with the vector pTHE10 to produce a transformant E. coli TDPDH(KCTC 0999BP); culturing the transformant in a medium under condition to induce the expression of the dTDP-glucose 4,6-dehydratase gene; and separating the dTDP-glucose 4,6-dehydratase from the cultured medium.
摘要翻译：目的：提供dTDP-葡萄糖4,6-脱水酶基因，含有相同基因的重组载体，其转化体和使用相同转化体的耐热性dTDP-葡萄糖4,6-脱水酶的制备方法。因此，耐热酶可用于医疗行业。构成：具有SEQ ID NO：1的核苷酸序列的dTDP-葡萄糖4,6-脱水酶基因来源于嗜热栖热菌GK24。重组载体pTHE10含有SEQ ID NO：1的dTDP-葡萄糖4,6-脱水酶基因。通过用载体pTHE10转化产生转化体大肠杆菌TDPDH（KCTC 0999BP）。制造耐热性dTDP-葡萄糖4,6-脱水酶的方法包括以下步骤：（1）将SEQ ID NO：1的dTDP-葡萄糖4,6-脱水酶基因插入表达载体中，制备重组载体pTHE10 ; 用载体pTHE10转化大肠杆菌以产生转化体大肠杆菌TDPDH（KCTC 0999BP）; 在培养基中培养转化体以诱导dTDP-葡萄糖4,6-脱水酶基因的表达; 并从培养基中分离dTDP-葡萄糖4,6-脱水酶。

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