专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

11. 发明公开

EP3362567A1 GENETICALLY ENGINEERED BACTERIUM COMPRISING ENERGY-GENERATING FERMENTATION PATHWAY 审中-公开
公开(公告)号：EP3362567A1
公开(公告)日：2018-08-22
申请号：EP16856229.6
申请日：2016-10-13
申请人： Lanzatech New Zealand Limited
发明人： KOEPKE, Michael , JENSEN, Rasmus, Overgaard , BEHRENDORFF, James Bruce Yarnton Haycock , HILL, Ryan Edward , JUMINAGA, Darmawi , MUELLER, Alexander, Paul
IPC分类号： C12N15/74 , C12N9/10 , C12N9/12 , C12P5/02 , C12P7/04 , C12P7/40 , C12P7/42 , C12P7/44
CPC分类号： C12P7/42 , C12N9/0006 , C12N9/0008 , C12N9/1029 , C12N9/1217 , C12N9/16 , C12P5/007 , C12P5/026 , C12P7/04 , C12P7/18 , C12P7/24 , C12P7/28 , C12P7/625 , C12Y101/01001 , C12Y101/01002 , C12Y102/07005 , C12Y203/01008 , C12Y203/01009 , C12Y203/01019 , C12Y207/02001 , C12Y207/02007 , C12Y301/0202 , Y02E50/343
摘要： The invention relates to a genetically engineered bacterium having an enzyme that converts 3-hydroxyisovaleryl-CoA to 3-hydroxyisovalerate and an enzyme that converts 3-hydroxyisovalerate to isobutylene. Typically, the bacterium is capable of producing isobutylene from a gaseous substrate containing CO, CO2, and/or H2, such as syngas or an industrial waste gas.

12. 发明公开

EP2602329A1 Biotechnologische Herstellung von 3-Hydroxyisobuttersäure 审中-公开
标题翻译：生物技术Herstellung von 3-Hydroxyisobuttersäure
公开(公告)号：EP2602329A1
公开(公告)日：2013-06-12
申请号：EP11191923.9
申请日：2011-12-05
申请人： Evonik Degussa GmbH
发明人： Haas, Thomas Dr. , Schaffer, Steffen Dr. , Pötter, Markus Dr. , Wessel, Mirja Dr. , Pfeffer, Jan Christoph Dr. , Gehring, Christian , Kirchner, Nicole , Wittmann, Eva-Maria
IPC分类号： C12P7/42 , C12N15/52 , C12N9/02
CPC分类号： C12P7/42 , C12N9/0004 , C12N9/001 , C12N9/1217 , C12N9/13 , C12N9/88 , C12N9/93 , C12N15/52 , C12Y103/99002 , C12Y207/02007 , C12Y402/01017 , C12Y602/01002
摘要： Die Erfindung betrifft ein Verfahren umfassend die Schritte a) Bereitstellen von Isobuttersäure, b) Kontaktieren von Isobuttersäure mit der Kombination aus Isobutyrat-Kinase und Phosphotransisobutyrylase und/oder Isobutyryl-Coenzym A-Synthetase/Ligase und/oder Isobutyrat-Coenzym A-Transferase, c) Kontaktieren des Produktes aus Schritt a) mit Isobutyryl-Coenzym A-Dehydrogenase, d) Kontaktieren des Produktes aus Schritt b) mit Methacrylyl-Coenzym A-Hydratase, und e) Hydrolyse des Produktes aus Schritt d) unter Bildung von 3-Hydroxyisobuttersäure, wobei wenigstens eines der Enzyme in Form einer Zelle verwendet wird, die eine ihrem Wildtyp gegenüber verringerte Aktivität einer 3-Hydroxyisobuttersäure-Dehydrogenase oder einer Variante davon umfasst, eine Zelle, die wenigstens ein Enzym aus der Gruppe umfassend Isobutyryl-Coenzym A-Synthetase/Ligase, Isobutyrat-Coenzym A-Transferase, Isobutyrat-Kinase, Phosphotransisobutyrylase, Isobutyryl-Coenzym A-Dehydrogenase, Methacrylyl-Coenzym A-Hydratase und 3-Hydroxisobutyryl-Coenzym A-Hydrolase und eine gegenüber ihrem Wildtyp verringerte Aktivität einer 3-Hydroxyisobuttersäure-Dehydrogenase oder einer Variante davon aufweist, wobei die Zelle bevorzugt zusätzlich eine Monooxygenase, noch bevorzugter eine Monooxygenase des AlkBGT-Typs oder eine Variante davon aufweist und die Verwendung einer solchen Zelle zur Herstellung von 3-Hydroxyisobuttersäure.
摘要翻译：生产3-羟基异丁酸包括：（a）使异丁酸与异丁酸酯激酶和磷酸转移异丁酰基酶和/或异丁酰辅酶A合成酶/连接酶和/或异丁酸酯辅酶A转移酶的组合接触; （b）使由步骤（a）获得的产物与异丁酰辅酶A脱氢酶接触; （c）使由步骤（b）获得的产物与甲基丙烯酰辅酶A水合酶接触; 和（d）水解由步骤（c）获得的产物，形成3-羟基异丁酸。生产3-羟基异丁酸包括：（a）使异丁酸与异丁酸酯激酶和磷酸转移异丁酰基酶和/或异丁酰辅酶A合成酶/连接酶和/或异丁酸酯辅酶A转移酶的组合接触; （b）使由步骤（a）获得的产物与异丁酰辅酶A脱氢酶接触; （c）使由步骤（b）获得的产物与甲基丙烯酰辅酶A水合酶接触; 和（d）水解由步骤（c）获得的产物，形成3-羟基异丁酸，其中至少一个步骤（a），（b）和（c））是使用以下形式的酶所述酶包括异丁酸酯激酶，磷酸转移异丁酰基酶，异丁酰辅酶A合成酶/连接酶，异丁酸酯辅酶A转移酶或3-羟基异丁酸脱氢酶或3-羟基异丁酸脱氢酶的变体的活性，以及活性相对于野生型细胞而减少。独立权利要求包括：（1）包含由异丁酰辅酶A合成酶/连接酶，异丁酸酯辅酶A转移酶，异丁酸酯激酶，磷酸转移异丁酰基酶，异丁酰辅酶A脱氢酶，甲基丙烯酰辅酶A 水解酶或3-羟基异丁酰辅酶A水解酶，其具有3-羟基异丁酸脱氢酶的活性或3-羟基异丁酸脱氢酶的变体，其中活性相对于野生型细胞而降低; 和（2）包含电池和异丁烷或异丁酸的反应混合物。

13. 发明公开

EP3377639A2 METHODS FOR PRODUCING ISOBUTENE FROM 3-METHYLCROTONIC ACID 审中-公开
公开(公告)号：EP3377639A2
公开(公告)日：2018-09-26
申请号：EP16809650.1
申请日：2016-11-17
申请人： Global Bioenergies , Scientist of Fortune S.A.
发明人： ALLARD, Mathieu , ANISSIMOVA, Maria , MARLIERE, Philippe
IPC分类号： C12P5/02 , C12P7/40 , C12N15/52 , C12N9/00 , C12N9/10 , C12N9/12 , C12N9/16 , C12N9/88
CPC分类号： C12P5/026 , C12N9/1029 , C12N9/1085 , C12N9/1217 , C12N9/13 , C12N9/16 , C12N9/88 , C12N9/93 , C12N15/52 , C12P7/40 , C12Y205/01 , C12Y207/02007 , C12Y207/02014 , C12Y207/02015 , C12Y208/03001 , C12Y208/03008 , C12Y301/02001 , C12Y301/02018 , C12Y301/0202 , C12Y401/01006 , C12Y401/01063 , C12Y604/01004 , C12Y604/01005
摘要： Described are methods for the production of isobutene comprising the enzymatic conversion of 3-methylcrotonic acid into isobutene wherein said 3-methylcrotonic acid is obtained by the enzymatic conversion of 3-methylcrotonyl-CoA into 3- methylcrotonic acid or wherein said 3-methylcrotonic acid is obtained by the enzymatic conversion of 3-hydroxyisovalerate (HIV) into 3-methylcrotonic acid. It is described that the enzymatic conversion of 3-methylcrotonic acid into isobutene can, e.g., be achieved by making use of a 3-methylcrotonic acid decarboxylase, preferably an FMN-dependent decarboxylase associated with an FMN prenyl transferase, an aconitate decarboxylase (EC 4.1.1.6), a methylcrotonyl-CoA carboxylase (EC 6.4.1.4), or a geranoyl-CoA carboxylase (EC 6.4.1.5).

14. 发明公开

EP3194605A1 METHODS FOR PRODUCING 3-HYDROXY-3-METHYLBUTYRIC ACID 审中-公开
标题翻译：生产3-羟基-3-甲基丁酸的方法
公开(公告)号：EP3194605A1
公开(公告)日：2017-07-26
申请号：EP15778881.1
申请日：2015-09-16
申请人： Global Bioenergies , Scientist of Fortune S.A.
发明人： MARLIERE, Philippe , ANISSIMOVA, Maria , ALLARD, Mathieu
IPC分类号： C12P7/52 , C12N15/70
CPC分类号： C12P7/52 , C12N9/1029 , C12N9/1217 , C12N9/88 , C12N15/52 , C12Y203/01008 , C12Y203/01019 , C12Y207/02001 , C12Y207/02007 , C12Y207/02014 , C12Y207/02015 , C12Y402/01017 , C12Y402/01018 , C12Y402/01055 , C12Y402/01059 , C12Y402/01074 , C12Y402/01116
摘要： Described is a method for the conversion of 3-methylcrotonyl-CoA into 3-hydroxy-3- methylbutyric acid comprising the steps of: (a) enzymatically converting 3-methylcrotonyl-CoA into 3-hydroxy-3-methylbutyryl-CoA; and (b) further enzymatically converting the thus produced 3-hydroxy-3-methylbutyryl-CoA into 3-hydroxy-3-methylbutyric acid wherein the enzymatic conversion of 3-hydroxy-3-methylbutyryl-CoA into 3-hydroxy-3-methylbutyric acid according to step (b) is achieved by first converting 3-hydroxy-3-methylbutyryl-CoA into 3-hydroxy-3-methylbutyryl phosphate and then subsequently converting the thus produced 3-hydroxy-3-methylbutyryl phosphate into 3-hydroxy-3-methylbutyric acid.
摘要翻译：描述了用于将3-甲基巴豆酰辅酶A转化为3-羟基-3-甲基丁酸的方法，其包括以下步骤：（a）将3-甲基巴豆酰辅酶A酶促转化为3-羟基-3-甲基丁酰辅酶A; （b）将由此产生的3-羟基-3-甲基丁酰-CoA进一步酶促转化成3-羟基-3-甲基丁酸，其中将3-羟基-3-甲基丁酰-CoA酶催化转化成3-羟基-3-甲基丁酸通过首先将3-羟基-3-甲基丁酰-CoA转化成3-羟基-3-甲基丁酰磷酸，然后随后将如此产生的3-羟基-3-甲基丁酰磷酸转化成3-羟基-3-甲基丁酰磷酸，实现根据步骤（b） 3-甲基丁酸。

15. 发明申请

US20170260548A1 METHOD FOR PRODUCING ISOBUTENE FROM 3-METHYLCROTONYL-COA 审中-公开
公开(公告)号：US20170260548A1
公开(公告)日：2017-09-14
申请号：US15511676
申请日：2015-09-16
申请人： Global Bioenergies , Scientist of Fortune, S.A.
发明人： Philippe Marlière , Mathieu Allard
IPC分类号： C12P5/02 , C12N9/10 , C12N9/12 , C12N9/02
CPC分类号： C12P5/026 , C12N9/0079 , C12N9/1029 , C12N9/1217 , C12P5/02 , C12Y114/15004 , C12Y203/01019 , C12Y207/02007
摘要： Described is a method for the production of isobutene from 3-methylcrotonyl-CoA comprising the steps of:(a) enzymatically converting 3-methylcrotonyl-CoA into 3-methylbutyric acid; and (b) further enzymatically converting the thus produced 3-methylbutyric acid into isobutene. The conversion of 3-methylcrotonyl-CoA into 3-methylbutyric acid can be achieved by first enzymatically converting 3-methylcrotonyl-CoA into 3-methylbutyryl-CoA and further enzymatically converting the thus produced 3-methylbutyryl-CoA into 3-methylbutyric acid. Alternatively, the conversion of 3-methylcrotonyl-CoA into 3-methylbutyric acid can be achieved by first enzymatically converting 3-methylcrotonyl-CoA into 3-methylcrotonic acid and then further enzymatically converting the thus produced 3-methylcrotonic acid into 3-methylbutyric acid.

16. 发明申请

US20170101631A1 GENETICALLY ENGINEERED BACTERIUM COMPRISING ENERGY-GENERATING FERMENTATION PATHWAY 有权
公开(公告)号：US20170101631A1
公开(公告)日：2017-04-13
申请号：US15293191
申请日：2016-10-13
申请人： LanzaTech New Zealand Limited
发明人： Michael Koepke , Rasmus Overgaard Jensen , James Bruce Yarnton Haycock Behrendorff , Ryan Edward Hill
IPC分类号： C12N9/12 , C12N9/02 , C12P5/00 , C12P7/04 , C12P7/42 , C12N9/10 , C12P7/28
CPC分类号： C12P7/42 , C12N9/0006 , C12N9/0008 , C12N9/1029 , C12N9/1217 , C12N9/16 , C12P5/007 , C12P5/026 , C12P7/04 , C12P7/18 , C12P7/24 , C12P7/28 , C12P7/625 , C12Y101/01001 , C12Y101/01002 , C12Y102/07005 , C12Y203/01008 , C12Y203/01009 , C12Y203/01019 , C12Y207/02001 , C12Y207/02007 , C12Y301/0202
摘要： The invention relates to a genetically engineered bacterium comprising an energy-generating fermentation pathway and methods related thereto. In particular, the invention provides a bacterium comprising a phosphate butyryltransferase (Ptb) and a butyrate kinase (Buk) (Ptb-Buk) that act on non-native substrates to produce a wide variety of products and intermediates. In certain embodiments, the invention relates to the introduction of Ptb-Buk into a C1-fixing microoorgansim capable of producing products from a gaseous substrate.

17. 发明申请

US20150299740A1 RECOMBINANT MICROORGANISM HAVING ENHANCED BUTANOL PRODUCING ABILITY AND METHOD FOR PRODUCING BUTANOL USING THE SAME 有权
标题翻译：具有增强的丁醇生产能力的重组微生物和使用其生产丁醇的方法
公开(公告)号：US20150299740A1
公开(公告)日：2015-10-22
申请号：US14418401
申请日：2013-03-11
申请人： GS CALTEX CORPORATION
发明人： Sang-Hyun LEE , Moon-Ho EOM
IPC分类号： C12P7/16 , C12N9/02 , C12N9/10 , C12N9/04
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/0008 , C12N9/1029 , C12N9/1217 , C12N9/13 , C12N15/52 , C12Y101/01001 , C12Y102/01003 , C12Y203/01008 , C12Y207/02007 , C12Y208/03008 , Y02E50/10 , Y02P20/52
摘要： The present invention relates to a microorganism having an acetyl CoA biosynthesis pathway and a butyryl CoA biosynthesis pathway; the microorganism being a recombinant microorganism having an increased ability to produce butanol, wherein a pathway for converting acetyl CoA into acetate is suppressed, and a pathway for converting acetate into acetyl CoA and a pathway for converting butyryl CoA into butanol are promoted. Also, the present invention concerns a method for producing butanol by using the recombinant microorganism.
摘要翻译：本发明涉及具有乙酰CoA生物合成途径和丁酰辅酶A生物合成途径的微生物; 所述微生物是具有增加生产丁醇能力的重组微生物，其中抑制将乙酰CoA转化为乙酸的途径，并且促进将乙酸转化为乙酰CoA的途径和将丁酰CoA转化为丁醇的途径。另外，本发明涉及使用重组微生物生产丁醇的方法。

18. 发明申请

US20150218600A1 BIOTECHNOLOGICAL PREPARATION OF 3-HYDROXYISOBUTYRIC ACID 审中-公开
标题翻译： 3-羟基丁酸的生物技术制备
公开(公告)号：US20150218600A1
公开(公告)日：2015-08-06
申请号：US14363178
申请日：2012-11-14
申请人： Thomas Haas , Steffen Schaffer , Markus Poetter , Mirja Wessel , Jan Christoph Pfeffer , Christian Gehring , Nicole Kirchner , Eva Maria Wittmann
发明人： Thomas Haas , Steffen Schaffer , Markus Poetter , Mirja Wessel , Jan Christoph Pfeffer , Christian Gehring , Nicole Kirchner , Eva Maria Wittmann
IPC分类号： C12P7/42 , C12N9/88 , C12N9/12 , C12N9/02 , C12N9/00 , C12N9/10
CPC分类号： C12P7/42 , C12N9/0004 , C12N9/001 , C12N9/1217 , C12N9/13 , C12N9/88 , C12N9/93 , C12N15/52 , C12Y103/99002 , C12Y207/02007 , C12Y402/01017 , C12Y602/01002
摘要： The invention relates to a method comprising the steps a) providing isobutyric acid, b) bringing isobutyric acid into contact with the combination of isobutyrate kinase and phosphotransisobutyrylase and/or isobutyryl-coenzyme A synthetase/ligase and/or isobutyrate-coenzyme A transferase, c) bringing the product from step a) into contact with isobutyryl-coenzyme A dehydrogenase, d) bringing the product from step b) into contact with methacrylyl-coenzyme A hydratase, and e) hydrolyzing the product from step d) to form 3-hydroxyisobutyric acid, where at least one of the enzymes is used in the form of a cell which, compared to its wildtype, comprises a reduced activity of a 3-hydroxyisobutyric acid dehydrogenase or a variant thereof, a cell which has at least one enzyme from the group comprising isobutyryl-coenzyme A synthetase/ligase, isobutyrate-coenzyme A transferase, isobutyrate kinase, phosphotransisobutyrylase, isobutyryl-coenzyme A dehydrogenase, methacrylyl-coenzyme A hydratase and 3-hydroxyisobutyryl-coenzyme A hydrolase and, compared to its wildtype, a reduced activity of a 3-hydroxyisobutyric acid dehydrogenase or a variant thereof, wherein the cell preferably has, in addition, a monooxygenase, more preferably a monooxygenase of the alkBGT type or a variant thereof and the use of such a cell for preparing 3-hydroxyisobutyric acid.
摘要翻译：本发明涉及包括以下步骤的方法：a）提供异丁酸，b）使异丁酸与异丁酸酯激酶和磷酸转移异丁酰基酶和/或异丁酰辅酶A合成酶/连接酶和/或异丁酸酯辅酶A转移酶c的组合接触）使来自步骤a）的产物与异丁酰辅酶A脱氢酶接触，d）将来自步骤b）的产物与甲基丙烯酰辅酶A水合酶接触，和e）水解步骤d）中的产物以形成3-羟基异丁酸酸，其中至少一种酶以细胞形式使用，与其野生型相比，其包含3-羟基异丁酸脱氢酶或其变体的降低的活性，具有至少一种酶的细胞异丁酰辅酶A合成酶/连接酶，异丁酸酯辅酶A转移酶，异丁酸酯激酶，磷酸转移异丁酰基酶，异丁酰辅酶A脱氢酶，甲基丙烯酰辅酶A水合酶和 3-羟基异丁酰辅酶A水解酶，与其野生型相比，3-羟基异丁酸脱氢酶或其变体的活性降低，其中细胞优选还具有单加氧酶，更优选alkBGT型的单加氧酶或其变体以及这种细胞用于制备3-羟基异丁酸的用途。

19. 发明公开

US20230372411A1 Methods and Compositions for Treatment of Microbiome Associated Disorders 审中-公开
公开(公告)号：US20230372411A1
公开(公告)日：2023-11-23
申请号：US18158047
申请日：2023-01-23
申请人： Pendulum Therapeutics, Inc.
发明人： Colleen CUTCLIFFE , John S. EID , Tomer ALTMAN , Orville G. KOLTERMAN , James H. BULLARD
IPC分类号： A61K35/74 , A61P25/22 , A61K9/28 , A61K31/733
CPC分类号： A61K35/74 , A61P25/22 , A61K9/28 , A61K31/733 , C12Y207/02007
摘要： Disclosed herein are methods and compositions for treatment of a microbiome associated disorder. Further, disclosed herein are methods and compositions for modulating short chain fatty acid production in a subject.

20. 发明授权

US09957531B1 Genetically engineered bacterium for the production of 3-hydroxybutyrate 有权
公开(公告)号：US09957531B1
公开(公告)日：2018-05-01
申请号：US15658668
申请日：2017-07-25
申请人： LanzaTech New Zealand Limited
发明人： Michael Koepke , Rasmus Overgaard Jensen , James Bruce Yarnton Haycock Behrendorff , Ryan Edward Hill , Darmawi Juminaga , Alexander Paul Mueller
IPC分类号： C12N1/20 , C12P7/42 , C12N9/10 , C12N9/02 , C12N9/04 , C12N9/12 , C12N9/16 , C12P7/24 , C12P7/18
CPC分类号： C12P7/42 , C12N9/0006 , C12N9/0008 , C12N9/1029 , C12N9/1217 , C12N9/16 , C12P5/007 , C12P5/026 , C12P7/04 , C12P7/18 , C12P7/24 , C12P7/28 , C12P7/625 , C12Y101/01001 , C12Y101/01002 , C12Y102/07005 , C12Y203/01008 , C12Y203/01009 , C12Y203/01019 , C12Y207/02001 , C12Y207/02007 , C12Y301/0202
摘要： The invention relates to a genetically engineered bacterium having an enzyme that converts acetyl-CoA to acetoacetyl-CoA, an enzyme that converts acetoacetyl-CoA to 3-hydroxybutyryl-CoA, and an enzyme that converts 3-hydroxybutyryl-CoA to 3-hydroxybutyrate. The bacterium may also have enzymes to produce other downstream products, such as 3-hydroxybutyryaldehyde, and 1,3-butanediol. Typically, the bacterium is capable of producing these products from a gaseous substrate, such as syngas or an industrial waste gas.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式