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11. 发明申请

WO2012174271A3 SYNTHETIC GENE CLUSTERS 审中-公开
标题翻译：合成基因组
公开(公告)号：WO2012174271A3
公开(公告)日：2013-06-13
申请号：PCT/US2012042502
申请日：2012-06-14
申请人： UNIV CALIFORNIA , MIRSKY ETHAN , TEMME KARSTEN , VOIGT CHRIS , ZHAO DEHUA
发明人： MIRSKY ETHAN , TEMME KARSTEN , VOIGT CHRIS , ZHAO DEHUA
IPC分类号： C12N15/09 , C12N15/31 , C12N15/63
CPC分类号： C12N15/635 , C07K14/255 , C07K14/26 , C12N9/0095 , C12N15/52 , C12N15/63 , C12N15/67 , C12N15/74 , C12Y118/06001 , G06F19/20 , G06F19/22
摘要： Methods for making synthetic gene clusters are described.
摘要翻译：描述制备合成基因簇的方法。

12. 发明公开

EP4389917A2 FUSION PROTEINS AND METHODS FOR STIMULATING PLANT GROWTH, PROTECTING PLANTS, AND IMMOBILIZING BACILLUS SPORES ON PLANTS 审中-实审
公开(公告)号：EP4389917A2
公开(公告)日：2024-06-26
申请号：EP24155327.0
申请日：2014-03-17
申请人： Spogen Biotech Inc.
发明人： THOMPSON, Brian , THOMPSON, Katie
IPC分类号： C12R1/00
CPC分类号： A01N37/46 , C07K14/32 , C07K2319/03520130101 , C12N9/0095 , C12N9/16 , C12N9/2402 , C12Y118/06001 , C12Y301/01003 , C12Y301/03008 , C12Y302/01004 , C12N9/20 , C12N9/2437 , C12N9/48 , C12Y304/00 , A01N63/50 , A01N63/22
摘要： The present invention is generally directed to fusion proteins containing a targeting sequence that targets the fusion protein to the exosporium of a Bacillus cereus family member. The invention also relates to recombinant Bacillus cereus family members expressing such fusion proteins and formulations containing the recombinant Bacillus cereus family members expressing the fusion proteins. Methods for stimulating plant growth, for protecting plants from pathogens, and for enhancing stress resistance in a plant by applying the recombinant Bacillus cereus family members or the formulations to plants or a plant growth medium are also described. The invention also relates to methods for immobilizing spores of a recombinant Bacillus cereus family member expressing a fusion protein on plants.

13. 发明公开

EP0130047A1 Recombinant DNA molecules 失效
标题翻译： Rekombinante DNS Molekule。
公开(公告)号：EP0130047A1
公开(公告)日：1985-01-02
申请号：EP84304191.4
申请日：1984-06-21
申请人： LUBRIZOL GENETICS INC.
发明人： Shine, John , Rolfe, Barry G. , Scott, Kieran F.
IPC分类号： C12N15/74 , C12N15/90 , C12N15/53 , C12N15/32
CPC分类号： C12N9/0095 , C07K14/325 , C07K14/825 , C12N15/743 , C12N15/90 , C12Y118/06001
摘要： There is provided a bacterial strain containing and replicating therein a recombinant DNA plasmid comprising:

(a) a vector
(b) a fragment of DNA controlling expression of a nitrogenase complex structural gene, and
(c) a structural gene under control of said fragment of DNA.
摘要翻译：提供了包含其中并复制重组DNA质粒的细菌菌株，其包含：（a）载体（b）控制氮酶复合物结构基因表达的DNA片段，和（c）在所述片段的控制下的结构基因脱氧核糖核酸。

14. 发明公开

US20230227827A1 ARTIFICIAL NON-CODING RNA MODULE FOR ENHANCING NITROGEN FIXATION ABILITY OF MICROORGANISMS 审中-公开
公开(公告)号：US20230227827A1
公开(公告)日：2023-07-20
申请号：US18008674
申请日：2020-07-06
申请人： BIOTECHNOLOGY RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES
发明人： Min LIN , Yuhua ZHAN , Yongliang YAN , Xiubin KE
IPC分类号： C12N15/113 , C12N15/74 , C12N9/02
CPC分类号： C12N15/113 , C12N15/74 , C12Y118/06001 , C12N9/0004 , C12N2510/02
摘要： An artificial non-coding RNA (ncRNA) module constructed by a synthetic biology technique and the use of the artificial ncRNA module in the construction of an artificial nitrogen fixation system are disclosed. The RNA module can enhance the post-transcriptional stability of nifHDK mRNA by interacting with a nitrogenase coding gene nifHDK mRNA, thereby improving the nitrogen fixation ability of a chassis microorganism. A fusion expression vector carrying the artificial RNA module is constructed and transformed into different chassis nitrogen-fixing microorganisms. It is confirmed through experiments that, under nitrogen fixation conditions, the artificial RNA module of the present disclosure can significantly improve the nitrogenase activity of a recombinant engineering bacterial strain.

15. 发明申请

US20160367950A1 A METHOD FOR PRODUCING NANOSTRUCTURED OR MICROSTRUCTURED MATERIALS AND A DEVICE FOR THEIR PRODUCTION 审中-公开
标题翻译：一种生产纳米结构或微结构材料的方法及其生产设备
公开(公告)号：US20160367950A1
公开(公告)日：2016-12-22
申请号：US14787599
申请日：2015-05-15
申请人： CESKE VYSOKE UCENI TECHNICKE V PRAZE
发明人： Milos BERAN , Frantisek TOMAN , Josef DRAHORAD , Jiri HOVORKA , Zdenek HUSEK
IPC分类号： B01F3/04 , B03C3/00 , B01D46/00 , B01D1/18 , F26B3/12 , B01D1/20 , C08J3/12 , C07K14/77 , A61K35/747 , A61K35/745 , A61K9/50 , C01D3/06 , C12N1/20 , C12N9/02 , B01D45/16
CPC分类号： B01F3/04021 , A61K9/5047 , A61K9/5089 , A61K35/745 , A61K35/747 , B01D1/18 , B01D1/20 , B01D45/16 , B01D46/00 , B01J2/04 , B03C3/00 , C01D3/06 , C07K14/77 , C08J3/12 , C08J2329/04 , C12N1/20 , C12N9/0095 , C12N2500/02 , C12Y118/06001 , F26B3/12
摘要： A device for producing nanostructured or microstructured with materials comprises a chamber in which a hollow shaft is assembled, at least one disc provided with an expansion gap. The hollow shaft has openings which connect the inner space of the hollow shaft with the expansion gap.A solution, emulsion or liquid suspension of substances or microorganisms optionally saturated with a gas, liquefied gas or supercritical liquid, is fed into an inner space of a disc through a hollow shaft. By means of the combination of a centrifugal force and fluid pressure occurs the outlet of the liquid through an expansion gap, to form microscopic droplets. The microscopic droplets are subsequently disintegrated by expansion of the gas to form an aerosol. The aerosol is subsequently dried by a drying gas stream to form solids.
摘要翻译：任选用气体，液化气体或超临界液体饱和的物质或微生物的溶液，乳液或液体悬浮液通过空心轴进料到盘的内部空间中。通过离心力和流体压力的组合，液体的出口通过膨胀间隙发生，形成微小液滴。随后微小的液滴通过膨胀气体而形成气溶胶而分解。随后通过干燥气流干燥气溶胶以形成固体。

16. 发明申请

US20160031948A1 FUSION PROTEINS AND METHODS FOR STIMULATING PLANT GROWTH, PROTECTING PLANTS, AND IMMOBILIZING BACILLUS SPORES ON PLANTS 有权
标题翻译：融合蛋白和刺激植物生长的方法，保护植物和植物上的苗木运动
公开(公告)号：US20160031948A1
公开(公告)日：2016-02-04
申请号：US14775892
申请日：2014-03-17
申请人： SPOGEN BIOTECH INC.
发明人： Brian Thompson , Katie Thompson
IPC分类号： C07K14/32 , A01N63/00 , C12N9/24 , C12N9/02 , C12N9/16
CPC分类号： C07K14/32 , A01N37/46 , A01N37/48 , A01N63/00 , A01N63/02 , C07K2319/035 , C12N9/0095 , C12N9/16 , C12N9/20 , C12N9/2402 , C12N9/2437 , C12N9/48 , C12Y118/06001 , C12Y301/01003 , C12Y301/03008 , C12Y302/01004 , C12Y304/00
摘要： The present invention is generally directed to fusion proteins containing a targeting sequence that targets the fusion protein to the exosporium of a Bacillus cereus family member. The invention also relates to recombinant Bacillus cereus family members expressing such fusion proteins and formulations containing the recombinant Bacillus cereus family members expressing the fusion proteins. Methods for stimulating plant growth, for protecting plants from pathogens, and for enhancing stress resistance in a plant by applying the recombinant Bacillus cereus family members or the formulations to plants or a plant growth medium are also described. The invention also relates to methods for immobilizing spores of a recombinant Bacillus cereus family member expressing a fusion protein on plants.
摘要翻译：本发明一般涉及含有将融合蛋白靶向蜡状芽孢杆菌家族成员的外植体的靶向序列的融合蛋白。本发明还涉及表达这种融合蛋白的重组蜡状芽孢杆菌家族成员和含有表达融合蛋白的重组蜡状芽孢杆菌家族成员的制剂。还描述了通过将重组蜡状芽孢杆菌家族成员或制剂施用于植物或植物生长培养基来刺激植物生长，保护植物免受病原体和增强植物胁迫抗性的方法。本发明还涉及将表达融合蛋白的重组蜡状芽孢杆菌家族成员的孢子固定在植物上的方法。

17. 发明申请

US20150225742A1 Methods and System for Photo-activated Hydrogen Generation 审中-公开
标题翻译：光活化氢生成方法与系统
公开(公告)号：US20150225742A1
公开(公告)日：2015-08-13
申请号：US14620386
申请日：2015-02-12
申请人： BOMAX Hydrogen LLC
发明人： Deborah B. Maxwell
IPC分类号： C12P3/00
CPC分类号： C12P3/00 , B82Y5/00 , C07K14/195 , C12N9/0095 , C12Y118/06001
摘要： Systems and methods for providing alternative fuel, in particular hydrogen photocatalytically generated by a system comprising photoactive nanoparticles and a nitrogenase cofactor are provided. In one aspect, the system includes a water soluble cadmium selenide nanoparticle (CdSe) surface capped with mercaptosuccinate (CdSe-MSA); and a NafY.FeMo-co complex comprising a NafY protein and an iron-molybdenum cofactor (FeMo-co); wherein the CdSe-MSA and the NafY.FeMo-co complex are present in about 1:1 molar ratio in a CdSe-MSA.NafY.FeMo-co system. In various embodiments, when illuminated, the CdSe-MSA.NafY.FeMo-co system is capable of photocatalytically producing hydrogen gas for an extended period of, e.g., at least 5 hours, at least 10 hours, or at least 90 hours. Methods for making and using the same are also provided.
摘要翻译：提供用于提供替代燃料，特别是由包含光敏纳米颗粒和氮酶辅因子的系统光催化产生的氢的系统和方法。在一个方面，该系统包括用巯基琥珀酸盐（CdSe-MSA）封端的水溶性硒化镉纳米颗粒（CdSe）表面; 和包含NafY蛋白质和铁 - 钼辅因子（FeMo-co）的NafY.FeMo-共轭复合物; 其中CdSe-MSA和NafY.FeMo-co络合物在CdSe-MSA.NafY.FeMo-co体系中以约1:1的摩尔比存在。在各种实施方案中，当照射时，CdSe-MSA.NafY.FeMo-co体系能够光催化生产氢气，持续延长的时间例如至少5小时，至少10小时或至少90小时。还提供了制造和使用它们的方法。

18. 发明申请

US20150101373A1 NITROGEN-FIXING BACTERIAL INOCULANT FOR IMPROVEMENT OF CROP PRODUCTIVITY AND REDUCTION OF NITROUS OXIDE EMISSION 有权
标题翻译：用于改善作物生产力和减少氧化氮排放的氮固定物质
公开(公告)号：US20150101373A1
公开(公告)日：2015-04-16
申请号：US14386673
申请日：2013-03-20
申请人： TEMASEK LIFE SCIENCES LABORATORY LIMITED
发明人： Madhaiyan Munusamy , Lianghui Ji
IPC分类号： C05F11/08 , C05D9/02 , A01N63/00 , C12N1/20 , C12R1/01
CPC分类号： C05F11/08 , A01N63/00 , C05D9/02 , C12N1/20 , C12N9/0095 , C12N9/0097 , C12R1/01 , C12Y118/06001 , C12Y119/06001 , Y02P60/218
摘要： The present invention relates to methods of reducing chemical fertilizer usage and greenhouse gas nitrous oxide emission and to methods of improving plant growth rate and seed productivity in agriculture through the application of a novel artificially manufactured formula containing a nitrogen-fixing bacterium that efficiently colonizes non-legume plants in aerial parts and the root system. The bacteria inocula and methods are particularly suitable for plants in the genera Jatropha, Sorghum, Gossypium, Elaeis, Ricinus, Oryza and Manihot.
摘要翻译：本发明涉及减少化肥使用和温室气体一氧化二氮排放的方法，以及通过应用含有固氮细菌的新型人工制剂，有效地定植非化学肥料的方法，提高农业中植物生长速率和种子生产力的方法，豆类植物在地上部分和根系。细菌接种物和方法特别适用于麻风树属，高粱属，棉子属，ae属，蓖麻属，Oryza和Manihot属的植物。

19. 发明专利

JP2016520525A 植物の生育を刺激し、植物を保護し、および、植物上にバチルスの芽胞を固定するための融合タンパク質および方法审中-公开
标题翻译：刺激植物生长，保护植物，和融合蛋白和方法用于保护植物的细菌芽孢
公开(公告)号：JP2016520525A
公开(公告)日：2016-07-14
申请号：JP2016502564
申请日：2014-03-17
申请人：スポジェン・バイオテック・インコーポレイテッドSpogen Biotech Inc. , スポジェン・バイオテック・インコーポレイテッドSpogen Biotech Inc.
发明人：ブライアン・トンプソン , ケイティ・トンプソン
IPC分类号： C07K19/00 , A01G7/00 , A01G7/06 , A01N25/08 , A01N63/02 , A01P21/00 , C07K14/32 , C07K14/325 , C07K14/37 , C12N1/20 , C12N1/21 , C12N15/09
CPC分类号： C07K14/32 , A01N37/46 , A01N37/48 , A01N63/00 , A01N63/02 , C07K2319/035 , C12N9/0095 , C12N9/16 , C12N9/20 , C12N9/2402 , C12N9/2437 , C12N9/48 , C12Y118/06001 , C12Y301/01003 , C12Y301/03008 , C12Y302/01004 , C12Y304/00
摘要：本発明は概して、融合タンパク質をBacillus cereusファミリーのメンバーのエキソスポリウムに向かわせる標的化配列を含有する融合タンパク質を目的とするものである。本発明はまた、そのような融合タンパク質を発現する組換えBacillus cereusファミリーのメンバー、および、当該融合タンパク質を発現する組換えBacillus cereusファミリーのメンバーを含有する製剤に関するものである。植物または植物生育環境に、当該組換えBacillus cereusファミリーのメンバー、または当該製剤を適用することによる、植物の生育を刺激する方法、病原体から植物を防御する方法、および、植物におけるストレス耐性を増強させる方法もまた記述される。本発明はまた、融合タンパク質を発現する組換えBacillus cereusファミリーのメンバーの芽胞を植物に固定する方法に関する。
摘要翻译：本发明一般是包含靶向序列的融合蛋白指导融合蛋白的蜡状芽孢杆菌家族目标外切运动钾成员。本发明还涉及重组蜡状芽孢杆菌家族表达这种融合蛋白的成员，和含有该重组蜡状芽孢杆菌家族表达融合蛋白的成员的制剂。所述植物或植物生境，通过应用重组蜡状芽孢杆菌的家庭成员或制剂中，刺激植物的生长，保护植物免受病原体的方法的方法，以及提高在植物中的胁迫耐受性方法也被描述。本发明还表达关于如何使植株固定的融合蛋白质的重组蜡状芽孢杆菌家族的孢子的成员。

20. 发明申请

WO2013141815A1 NITROGEN-FIXING BACTERIAL INOCULANT FOR IMPROVEMENT OF CROP PRODUCTIVITY AND REDUCTION OF NITROUS OXIDE EMISSION 审中-公开
标题翻译：用于改善作物生产力和减少氧化氮排放的氮固定物质
公开(公告)号：WO2013141815A1
公开(公告)日：2013-09-26
申请号：PCT/SG2013/000112
申请日：2013-03-20
申请人： TEMASEK LIFE SCIENCES LABORATORY LIMITED
发明人： MUNUSAMY, Madhaiyan , Jl, Lianghui
IPC分类号： C12N1/20 , A01N63/00
CPC分类号： C05F11/08 , A01N63/00 , C05D9/02 , C12N1/20 , C12N9/0095 , C12N9/0097 , C12R1/01 , C12Y118/06001 , C12Y119/06001 , Y02P60/218
摘要： The present invention relates to methods of reducing chemical fertilizer usage and greenhouse gas nitrous oxide emission and to methods of improving plant growth rate and seed productivity in agriculture through the application of a novel artificially manufactured formula containing a nitrogen-fixing bacterium that efficiently colonizes non-legume plants in aerial parts and the root system. The bacteria inocula and methods are particularly suitable for plants in the genera Jatropha, Sorghum, Gossypium, Elaeis, Ricinus, Oryza and Manihot.
摘要翻译：本发明涉及减少化肥使用和温室气体一氧化二氮排放的方法，以及通过应用含有固氮细菌的新型人工制剂，有效地定植非化学肥料的方法，提高农业中植物生长速率和种子生产力的方法，豆类植物在地上部分和根系。细菌接种物和方法特别适用于麻风树属，高粱属，棉子属，ae属，蓖麻属，Oryza和Manihot属的植物。

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