专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

11. 发明申请

WO1993008307A1 PRESSURE CONTROL SYSTEM FOR A BIOREACTOR 审中-公开
标题翻译：一种生物反应器的压力控制系统
公开(公告)号：WO1993008307A1
公开(公告)日：1993-04-29
申请号：PCT/US1992008695
申请日：1992-10-16
申请人： ENDOTRONICS, INC. , TECNOMARA DEUTSCHLAND GMBH
发明人： ENDOTRONICS, INC. , TECNOMARA DEUTSCHLAND GMBH , WILSON, John, R. , KOWOL, Ewald
IPC分类号： C12Q03/00
CPC分类号： C12M41/40
摘要： A pressure control system for a bioreactor of the type having a gas space and a cell culture space therein is supplied with a gas such as oxygen separately from the supply of liquid nutrient media. The pressures in the gas space and the pressure in the cell culture space are controlled so that a higher pressure is maintained in the cell culture space than in the gas space to prevent displacement of the liquid nutrient media.

12. 发明申请

WO1987006610A1 METHOD OF CULTURING LEUKOCYTES 审中-公开
标题翻译：培养白藜芦醇的方法
公开(公告)号：WO1987006610A1
公开(公告)日：1987-11-05
申请号：PCT/US1987000924
申请日：1987-04-27
申请人： ENDOTRONICS, INC.
发明人： ENDOTRONICS, INC. , MELINK, Georgiann, B. , SHANKAR, Raji, A.
IPC分类号： C12N05/00
CPC分类号： C12M25/10 , C12M29/16 , C12N5/0636 , C12N2501/23
摘要： A method for culturing leukocytes includes the culturing of leukocytes with interleukin-2 in a hollow fiber cartridge perfusion system (16) for at least four days to 19 days to achieve a harvest yield of at least 100% to 5000%. The leukocytes are recovered and have a lytic activity comparable to that of cells grown in a static culturing system.
摘要翻译：培养白细胞的方法包括在中空纤维素素灌注系统（16）中培养白细胞介素-2至少4天至19天，以达到至少100％至5000％的收获产量。回收白细胞并具有与在静态培养系统中生长的细胞相当的溶解活性。

13. 发明申请

WO1986002378A1 HOLLOW FIBER CELL CULTURE DEVICE AND METHOD OF OPERATION 审中-公开
标题翻译：中空纤维细胞培养装置及其操作方法
公开(公告)号：WO1986002378A1
公开(公告)日：1986-04-24
申请号：PCT/US1985001948
申请日：1985-10-09
申请人： ENDOTRONICS, INC.
发明人： ENDOTRONICS, INC. , CRACAUER, Ray, F. , WALKER, Robert, D. , GRUENBERG, Micheal, L.
IPC分类号： C12N05/00
CPC分类号： C12M29/16 , C12M25/10 , C12M29/18 , C12M41/00 , C12M41/40
摘要： An improved cell culturing device includes a hollow fiber cartridge (11) having a shell (16) and a plurality of capillaries (12) extending through the shell with at least some of the capillaries (12) having selectively permeable walls. A cell culturing space (14) is located between the shell (16) and capillaries (12). The improvement includes a chamber (32) containing a second medium supply (33) fluidly connected to the cell culturing space. A pressurizing system (46) pressurizes the medium within the chamber to a level higher than the level of medium flowing through the lumen of the capillaries. A valving mechanism (38, 40) alternatively and selectively restricts flow of medium between the chamber and the conduits such that circulation is effected in the cell culturing space (14).
摘要翻译：改进的细胞培养装置包括具有壳体（16）的中空纤维筒（11）和延伸穿过壳体的多个毛细管（12），其中至少一些毛细管（12）具有选择性可渗透的壁。细胞培养空间（14）位于壳体（16）和毛细管（12）之间。该改进包括包含流体连接到细胞培养空间的第二介质供应（33）的室（32）。加压系统（46）将腔室内的介质加压到高于流过毛细血管内腔的介质水平的水平。阀机构（38,40）交替或选择性地限制腔室和管道之间的介质流动，使得在细胞培养空间（14）中进行循环。

14. 发明授权

US5631006A Immunotherapy protocol of culturing leukocytes in the presence of interleukin-2 in a hollow fiber cartridge 失效
标题翻译：在白介素-2存在下培养白细胞的免疫疗法在中空纤维素盒中
公开(公告)号：US5631006A
公开(公告)日：1997-05-20
申请号：US483876
申请日：1995-06-07
申请人： Georgiann B. Melink , Raji A. Shankar
发明人： Georgiann B. Melink , Raji A. Shankar
IPC分类号： C12M3/00 , C12M3/06 , C12N5/0783 , A61K35/12 , A61K35/14 , A61K35/26 , C12N5/08
CPC分类号： C12M25/10 , C12M29/16 , C12N5/0636 , C12N2501/23
摘要： An immunotherapy protocol in which leukocytes are cultured in the presence of interleukin-2 in a hollow fiber cartridge perfusion system for at least four days to achieve a harvest yield of at least 100% of leukocytes with respect to the number of cells initially present in the hollow cartridge, and where the leukocytes have a lytic activity at least equal to that of cells grown in a static culturing system and where the lytic activity of the cells following the culture period in the hollow fiber is at least equal to cells grown in a static culturing system.
摘要翻译：一种免疫治疗方案，其中将白细胞在白细胞介素-2存在下在中空纤维素滤器灌注系统中培养至少四天，以获得相对于最初存在于细胞中的细胞数量至少100％的白细胞的收获产量并且其中白细胞具有至少等于在静态培养系统中生长的细胞的裂解活性，并且其中中空纤维中培养期后的细胞的裂解活性至少等于在静态培养物中生长的细胞培养体系。

15. 发明申请

WO1988001643A1 METHOD OF CULTURING CELLS 审中-公开
标题翻译：培养细胞的方法
公开(公告)号：WO1988001643A1
公开(公告)日：1988-03-10
申请号：PCT/US1987002089
申请日：1987-08-21
申请人： ENDOTRONICS, INC.
发明人： ENDOTRONICS, INC. , SOURS, David, P. , ANDERSEN, Bradley, G.
IPC分类号： C12N05/00
CPC分类号： C12N5/163
摘要： Cells are cultured in a continuous culture system wherein glucose delivery and pH maintenance are based on values determined in a static culture of the cell line of interest. To provide maximum growth of the cell line in the continuous culture system, a pH level is maintained in the continuous culturing system corresponding to a pH value occurring at a point in time of greatest percentage increase in cell number in a static culture of the cell line of interest. The glucose and lactate levels are maintained in the continuous culture system corresponding to glucose and lactate levels at a point in time of greatest quantitative increase in cell number in the static culture of the cell line of interest. If the cell line of interest is a cell line used to produce a useful biological product, then the pH set point is changed at a maximum cell count to a value corresponding to a pH value occurring at a time wherein the cell count is at a maximum in the static culture and the glucose and lactate set points are changed to values corresponding to a point in time where the production of the biological product per cell per time period is greatest in the static culture.
摘要翻译：细胞在连续培养系统中培养，其中葡萄糖递送和pH维持基于在感兴趣的细胞系的静态培养物中测定的值。为了在连续培养系统中提供细胞系的最大生长，在连续培养系统中保持pH水平，对应于在细胞系的静态培养物中细胞数量增加最大百分比增加的时间点发生的pH值出于兴趣。葡萄糖和乳酸盐水平保持在对应于葡萄糖和乳酸盐水平的连续培养系统中，在感兴趣的细胞系的静态培养物中细胞数量最大定量增加的时间点。如果感兴趣的细胞系是用于产生有用的生物产物的细胞系，则将pH设定点以最大细胞计数改变为对应于细胞计数最大值时出现的pH值的值在静态培养物中，将葡萄糖和乳酸盐的设定点改变为对应于在静态培养物中，每个细胞每个细胞每个细胞的生物体产生最大的时间点的值。

16. 发明申请

WO1986002379A1 HOLLOW FIBER CULTURE DEVICE FOR IMPROVED NUTRIENT PERFUSION AND PRODUCT CONCENTRATION AND METHOD OF OPERATION 审中-公开
标题翻译：中空纤维培养装置，用于改善营养吸收和产品浓度和操作方法
公开(公告)号：WO1986002379A1
公开(公告)日：1986-04-24
申请号：PCT/US1985001949
申请日：1985-10-09
申请人： ENDOTRONICS, INC.
发明人： ENDOTRONICS, INC. , WALKER, Robert, D. , GRUENBERG, Michael, L. , CRACAUER, Ray, F.
IPC分类号： C12N05/00
CPC分类号： C12M29/16 , C12M25/10 , C12M29/18 , C12M47/10
摘要： A hollow fiber culture device includes a first hollow fiber cartridge (12) and a second hollow fiber cartridge (14), the capillaries of which (18) are fluidly connected in series by a fluid connection (36) having a flow restriction therein (38). The flow restriction (38) provides a pressure drop between the capillaries of the first (18) and second (28) cartridges and provides back pressure to the cappilaries of the first hollow fiber cartridge so that medium is perfused under ultrafiltrative conditions. The extracapillary space (34) of the first hollow fiber cartridge is fluidly connected to the extracapillary space of the second hollow fiber cartridge (14) so that waste- and product-containing medium is conveyed to the extracapillary space (34) of the second hollow fiber cartridge wherein the product is concentrated and the waste-containing medium is diffused into the lumen of the capillaries.
摘要翻译：中空纤维培养装置包括第一中空纤维筒（12）和第二中空纤维筒（14），其毛细管（18）通过其中具有流动限制的流体连接（36）流体连接（38））。流动限制（38）在第一（18）和第二（28）盒的毛细管之间提供压降，并且向第一中空纤维筒的帽状物提供背压，使得在超滤条件下灌注介质。第一中空纤维筒的毛细血管空间（34）流体地连接到第二中空纤维盒（14）的毛细血管外空间，使得含废物和产品的培养基被输送到第二中空纤维筒（14）的毛细血管空间（34）其中产品被浓缩并且含废物的介质扩散到毛细管的内腔中。

17. 发明申请

WO1984000028A1 APPARATUS FOR DELIVERING A CONTROLLED DOSAGE OF A CHEMICAL SUBSTANCE 审中-公开
标题翻译：提供化学物质控制剂量的装置
公开(公告)号：WO1984000028A1
公开(公告)日：1984-01-05
申请号：PCT/US1983000865
申请日：1983-05-31
申请人： ENDOTRONICS, INC.
发明人： ENDOTRONICS, INC. , GRUENBERG, Michael, L.
IPC分类号： B67D05/08
CPC分类号： C12M33/07 , A01N1/0247 , C12M35/08 , C12M41/26 , C12M41/34 , C12M41/48
摘要： An apparatus delivers a dosage of a chemical substance preferably to a biological tissue in such a controlled manner that the concentration affecting the biological tissue is known at each end every point in time. The apparatus includes a plurality of vessels (20) with each vessel (20) containing a different concentration of the chemical substance. A valve (34) has a plurality of inlets (36) and a single outlet (38) wherein each inlet (36) is connected to a corresponding vessel (20). The biological tissue is preferably held within a chamber (42a) for treatment with the chemical substance. A pump (40) positioned preferably between the valve (34) and the biological tissue provides a transport force for delivering the chemical substance to the tissue. The valve (34) and the pump (40) are controlled by a computer control system (12) which determines which inlet (36) of the valve (34) is fluidly connected to the outlet (38) for providing a preselected concentration of the chemical substance to the tissue such that the concentration of the chemical substance affecting the tissue is controlled at each and every point in time.
摘要翻译：一种装置以这样的受控方式将化学物质的剂量优选递送到生物组织，使得在每个时间点的每一端都知道影响生物组织的浓度。该装置包括多个容器（20），每个容器（20）含有不同浓度的化学物质。阀（34）具有多个入口（36）和单个出口（38），其中每个入口（36）连接到对应的容器（20）。生物组织优选保持在用于用化学物质处理的室（42a）内。优选位于阀（34）和生物组织之间的泵（40）提供用于将化学物质输送到组织的输送力。阀（34）和泵（40）由计算机控制系统（12）控制，计算机控制系统（12）确定阀（34）的哪个入口（36）流体地连接到出口（38），以提供预选的浓度化学物质到组织，使得影响组织的化学物质的浓度在每个时间点被控制。

18. 发明申请

WO1983002953A1 PROCESS AND APPARATUS FOR CONTROLLING PATTERNS OF CHEMICALS STIMULI ADMINISTRATION TO BIOLOGICAL TISSUE 审中-公开
标题翻译：用于控制化学品刺激性生物组织管理模式的方法和装置
公开(公告)号：WO1983002953A1
公开(公告)日：1983-09-01
申请号：PCT/US1983000090
申请日：1983-01-21
申请人： ENDOTRONICS, INC.
发明人： ENDOTRONICS, INC. , GRUENBERG, Micheal, L.
IPC分类号： C12N05/00
CPC分类号： C12M35/08 , A01N1/021 , A01N1/0226
摘要： A process mimics in-vivo or other conditions to a biological tissue that has been isolated from its natural nutrient supply by controlling the concentration of chemical stimuli delivered to the tissue. The process includes starting with a first known volume of the supportive media, having a known concentration of chemical stimuli affecting the tissue at an initial known point in time. Simultaneously, a first known volume and known amount of the chemical stimuli is removed from affecting the tissue and a second known amount of the chemical stimuli is added in a known volume having a known concentration different than the concentration of the chemical stimuli initially affecting the tissue. The volume of the supportive media is then determined along with the resulting concentrations of the chemical stimuli affecting the tissue. The above steps are repeated resulting in the concentration that affects the tissue being known at each and every point in time. In delivering the chemical stimuli to the tissue, the volume of the supportive media supplied to the tissue, the rate of delivery, rate of removal of the media along with the initial concentration of the chemical stimuli and the concentration of the chemical stimuli being supplied is used to determine a desired administration of the chemical stimuli such that the concentration affecting the tissue emulates the desired fluctuations of the chemical stimuli with respect to time. With these parameters defined, the concentration affecting the tissue with respect to time is known at each and every point in time. An apparatus for carrying out the above process is disclosed.
摘要翻译：过程通过控制递送到组织的化学刺激的浓度，将生物组织体内或其他条件模拟为从其天然营养供应中分离的生物组织。该方法包括从第一已知体积的支持介质开始，其在初始已知时间点具有影响组织的已知浓度的化学刺激。同时，将第一已知体积和已知量的化学刺激从影响组织中去除，并且将第二已知量的化学刺激物加入到具有不同于最初影响组织的化学刺激的浓度的已知浓度的已知体积中。然后确定支持介质的体积以及影响组织的化学刺激的所得浓度。重复上述步骤，导致在每个时间点影响已知组织的浓度。在将化学刺激递送到组织中时，提供给组织的支持介质的体积，输送速率，介质的去除速率以及化学刺激的初始浓度和所提供的化学刺激的浓度是否为用于确定化学刺激的所需施用，使得影响组织的浓度相对于时间模拟化学刺激的期望波动。在定义了这些参数的情况下，在每个时间点都知道影响组织相对于时间的浓度。公开了一种用于执行上述过程的装置。

19. 发明专利

DE59208421D1 未知
公开(公告)号：DE59208421D1
公开(公告)日：1997-06-05
申请号：DE59208421
申请日：1992-09-30
申请人： INTEGRA BIOSCIENCES GMBH , ENDOTRONICS INC
发明人： WILSON JOHN R , KOWOL EWALD C O TECNOMARA DEUT
IPC分类号： C12M1/04 , C12M3/06 , C12M3/04
摘要： The invention relates to a pressure control system for an apparatus for cell cultivation and isolation of cell products (bioreactor), having a gas chamber (32) and having a cell culture chamber (26) located therein, as well as devices for gas introduction (64) and gas removal (66) which are connected to the apparatus, furthermore having devices (78) for introducing liquid nutrients and for removing liquid waste products (76), which are attached to the apparatus separate from the devices for gas introduction and gas removal. The means (68, 69) for controlling the gas pressure in the gas chamber (32) are functionally connected to the bioreactor in order to maintain a pressure in the cell culture chamber (26) which is higher than that in the gas chamber (32). In a second variant of the invention, the means (68, 82) for controlling the gas pressure in the cell culture chamber (26) are functionally connected to the bioreactor in order to maintain a pressure in the cell culture chamber (26) which is higher than that in the gas chamber (32). The devices for removing gas have a vacuum pump (69) and the devices for introducing liquid nutrients have a nutrient source (78) and pumps. The devices for removing liquid waste materials are provided with means (76) for collecting liquid materials, which are located underneath the apparatus so that syphon outflow is present.

20. 发明专利

CA1270455A APPARATUS AND METHOD FOR CULTURING CELLS, REMOVING WASTE AND CONCENTRATING PRODUCT 未知
公开(公告)号：CA1270455A
公开(公告)日：1990-06-19
申请号：CA522096
申请日：1986-11-04
申请人： ENDOTRONICS INC
发明人： HARM WILLIAM H , HIRSCHEL MARK D , GRUENBERG MICHEAL L
IPC分类号： C12M1/12 , C12M3/00 , C12M3/06 , C12N5/00
摘要： APPARATUS AND METHOD FOR CULTURING CELLS, REMOVING WASTE AND CONCENTRATING PRODUCT An apparatus and method for culturing cells includes a waste removal and product concentrating loop in which waste is removed from the medium and product produced by the cells is concentrated for harvesting. The apparatus includes a reservoir for retaining a supply of medium. The reservoir is in fluid communication with a cell culturing loop wherein medium flows from the reservoir to the cells through gas-diffusable tubing disposed partially in a heating and gassing block and back to the reservoir. The medium waste removal and concentrating loop removes waste components produced by the cells and is in fluid communication with the reservoir such that medium is transferred from the reservoir to the waste removal and concentrating loop and back to the reservoir. The waste removal and concentrating loop includes a device having a semipermeable membrane. The medium flows along one side of the membrane for selective transfer of the waste components through the membrane. A mechanism for selectively producing a back pressure in the medium is included downstream of the membrane and aids in transfer of waste components through the membrane to remove the waste components from the medium.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式