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11. 发明申请

US20090012714A1 Test of amino acid sequence constituting peptide using isotopic ratio 审中-公开
标题翻译：使用同位素比测试构成肽的氨基酸序列
公开(公告)号：US20090012714A1
公开(公告)日：2009-01-08
申请号：US11659113
申请日：2005-08-04
申请人： Yasushi Ishihama , Takatoshi Kawai
发明人： Yasushi Ishihama , Takatoshi Kawai
IPC分类号： G01N33/68
CPC分类号： G16B50/00 , G16B30/00
摘要： It is an object of the present invention, when determining and identifying an amino acid sequence of a peptide using MS, to obtain additional information from the MS for evaluating validity of an amino acid sequence in a candidate list outputted from an identifying engine. The present invention provides a method of testing an amino acid sequence inferred by searching a peptide-related database based on peptide mass information and/or peptide modification information obtained through mass spectrometry on a peptide, the method comprising the steps: (1) calculating a theoretical value of an isotopic ratio for the peptide from the inferred amino acid sequence and/or the peptide modification information; (2) measuring a measured value of the isotopic ratio for the peptide from the peptide mass information; and (3) comparing the theoretical value and the measured value, and evaluating validity of the inferred amino acid sequence from differences between the theoretical value and the measured value.
摘要翻译：本发明的目的是，当使用MS确定和鉴定肽的氨基酸序列时，从MS中获得用于评估从识别引擎输出的候选列表中的氨基酸序列的有效性的附加信息。本发明提供了一种通过基于肽质量信息和/或通过质谱法获得的肽修饰信息在肽上搜索肽相关数据库来推断的氨基酸序列的方法，所述方法包括以下步骤：（1）计算来自推断的氨基酸序列和/或肽修饰信息的肽的同位素比的理论值; （2）从肽质量信息测量肽的同位素比的测定值; 和（3）比较理论值和测量值，并根据理论值和测量值之间的差异评估推断的氨基酸序列的有效性。

12. 发明申请

US20080319676A1 Absolute Quantitation of Protein Contents Based on Exponentially Modified Protein Abundance Index by Mass Spectrometry 有权
标题翻译：基于指数变化的蛋白质丰度指数的蛋白质内容的绝对定量
公开(公告)号：US20080319676A1
公开(公告)日：2008-12-25
申请号：US11658592
申请日：2005-07-04
申请人： Yasushi Ishihama
发明人： Yasushi Ishihama
IPC分类号： G01N33/68
CPC分类号： G01N33/6803 , G01N33/6848
摘要： The present inventor has established protein abundance index (PAI, π) to determine the protein contents in a protein mixture solution using nanoLC-MSMS data. Digested peptides were analyzed by nanoLC-MS/MS and the obtained results were applied to a Mascot protein identification algorism based on tandem mass spectra. PAI is defined as the number of observed peptides divided by the number of observable peptides per protein. PAI from different concentrations of serum albumin showed linear relationship to the logarithm of the protein concentration. This was also valid for 47 proteins in a mouse whole cell lysate analyzed by single run of nanoLC-MS/MS. On the other hand, Mascot protein scores as well as the number of identified peptides per protein were less correlated to the protein abundance. For absolute quantitation, PAI was converted to exponentially modified PAI (EMPAI, mπ), which is proportional to protein contents in the protein mixture. For the 47 proteins in the whole lysate, the deviation percentages of the EMPAI-based concentrations to the actual values were within 63% in average. EMPAI was successfully applied to comprehensive protein expression analysis and performed a comparison study between gene and protein expression in an HCT116 human cancer cells. Accordingly, the present invention provides a method and a computer program for quantifying the protein contents based on the protein abundance index.
摘要翻译：本发明人利用nanoLC-MSMS数据建立了蛋白质丰度指数（PAI，pi），以确定蛋白质混合溶液中的蛋白质含量。通过纳米LC-MS / MS分析消化肽，将得到的结果应用于基于串联质谱的吉祥物蛋白鉴定算法。 PAI定义为观察到的肽数除以每个蛋白质的可观察肽数。不同浓度血清白蛋白的PAI与蛋白质浓度的对数呈线性关系。这也对通过单次纳米LC-MS / MS分析的小鼠全细胞裂解物中的47种蛋白质有效。另一方面，Mascot蛋白质分数以及每个蛋白质鉴定的肽数量与蛋白质丰度较不相关。为了绝对定量，将PAI转化为指数变化的PAI（EMPAI，mpi），其与蛋白质混合物中的蛋白质含量成比例。对于整个裂解物中的47种蛋白质，EMPAI基浓度与实际值的偏差百分比在63％以内。 EMPAI成功应用于综合蛋白表达分析，并在HCT116人类癌细胞中进行基因与蛋白质表达的比较研究。因此，本发明提供了一种用于基于蛋白质丰度指数量化蛋白质含量的方法和计算机程序。

13. 发明申请

US20060048367A1 System and method of manufacturing tip device 审中-公开
标题翻译：制造尖端装置的系统和方法
公开(公告)号：US20060048367A1
公开(公告)日：2006-03-09
申请号：US11212174
申请日：2005-08-26
申请人： Yasushi Ishihama , Takashi Usui
发明人： Yasushi Ishihama , Takashi Usui
IPC分类号： B23P11/00
CPC分类号： B01L3/0275 , B01L2200/12 , B01L2300/0681 , G01N2030/009 , G01N2035/1053 , Y10T29/49833 , Y10T29/5116
摘要： A filter filling apparatus serving as a system for manufacturing a tip device according to the present invention comprises, for example, on a base inside a casing, a sheet holder with a sheet-like filter carried thereon and a tip rack where a plurality of tips are held in a matrix-like configuration. Furthermore, a small-diameter filter punching-discharging mechanism having a plurality of small-diameter pipes disposed in a row and movable in the vertical direction and a filter push-in mechanism having a plurality of small-diameter rods disposed in a row and movable in the vertical direction are provided above the sheet holder and the tip rack. A cylinder connected to a nitrogen gas supply unit is connected via piping to the small-diameter pipes.
摘要翻译：作为根据本发明的用于制造尖端装置的系统的过滤器填充装置包括例如在壳体内的基部上，其上承载有片状过滤器的片保持器和其中多个尖端被保持在矩阵状配置中。此外，具有排列成行并可在垂直方向上移动的多个小直径管的小直径过滤器冲压排放机构以及具有排列成行并可移动的多个小直径杆的过滤推入机构在垂直方向上设置在片保持器和顶端架上方。连接到氮气供应单元的气缸通过管道连接到小直径管道。

14. 发明授权

US6140321A Polymorphs of donepezil hydrochloride and process for production 失效
标题翻译：盐酸多奈哌齐的多晶型物及其制备方法
公开(公告)号：US6140321A
公开(公告)日：2000-10-31
申请号：US774802
申请日：1996-12-30
申请人： Akio Imai , Hideaki Watanabe , Takashi Kajima , Yasushi Ishihama , Akiyo Ohtsuka , Tomohide Tanaka
发明人： Akio Imai , Hideaki Watanabe , Takashi Kajima , Yasushi Ishihama , Akiyo Ohtsuka , Tomohide Tanaka
IPC分类号： C07D211/32 , A61K31/445 , A61P25/28 , A61P43/00 , B01D9/02 , A01N43/46 , A01N43/36 , C07D211/20 , C07D223/00
CPC分类号： C07D211/32
摘要： Donepezil hydrochloride, 1-benzyl-4-[(5,6-dimethoxy-1-indanon)-2-yl]methylpiperidine hydrochloride, is provided here in the form of four polymorphs which are stable against heat and humidity in the pharmaceutical use. They can be industrially produced. They are specified by peaks in X-ray powder diffraction pattern and absorption peaks in infrared absorption spectra in potassium bromide.
摘要翻译：盐酸多奈哌齐，1-苄基-4 - [（5,6-二甲氧基-1-茚满酮）-2-基]甲基哌啶盐酸盐，以药物用途中耐热和湿气稳定的四种多晶型物的形式提供。他们可以在工业上生产。它们由X射线粉末衍射图中的峰和溴化钾红外吸收光谱中的吸收峰指定。

15. 发明授权

US6017973A Photocurable resin composition, method of producing photo-cured shaped object, vacuum casting mold, vacuum casting method and novel urethane acrylate 失效
标题翻译：光固化树脂组合物，光硬化成形体的制造方法，真空铸模，真空铸造法和新型聚氨酯丙烯酸酯
公开(公告)号：US6017973A
公开(公告)日：2000-01-25
申请号：US857639
申请日：1997-05-16
申请人： Yorikazu Tamura , Tsuneo Hagiwara , Yasushi Ishihama , Minoru Kayanoki , Takakuni Ueno
发明人： Yorikazu Tamura , Tsuneo Hagiwara , Yasushi Ishihama , Minoru Kayanoki , Takakuni Ueno
IPC分类号： B29C39/00 , B29C39/42 , C08F2/50 , C08F222/10 , G03F7/027 , C08G18/10 , G03C1/73 , G03C9/08
CPC分类号： C08F222/1006 , B29C39/003 , B29C39/42 , G03F7/027
摘要： A photocurable resin composition comprising (A) at least one urethane di- or triacrylate, which is a novel compound, (B) a radical polymeriable compound, and (C) a photopolymerization initiator, the weight ratio of the urethane acrylate (A) to the radical polymerizable compound (B) being 65/35 to 25/75; a method of producing a photo-cured object by photo-curing the above composition; a vacuum casting mold obtained by the above method; and a vacuum casting method using the above mold.
摘要翻译：一种光固化树脂组合物，其包含（A）至少一种作为新化合物的氨基甲酸酯二 - 或三丙烯酸酯，（B）自由基可聚合化合物和（C）光聚合引发剂，所述氨基甲酸酯丙烯酸酯（A）与自由基聚合性化合物（B）为65/35〜25/75; 通过光固化上述组合物来制造光固化物体的方法; 通过上述方法获得的真空铸模; 和使用上述模具的真空铸造方法。

16. 发明授权

US5985864A Polymorphs of donepezil hydrochloride and process for production 失效
公开(公告)号：US5985864A
公开(公告)日：1999-11-16
申请号：US870394
申请日：1997-06-06
申请人： Akio Imai , Hideaki Watanabe , Takashi Kajima , Yasushi Ishihama , Akiyo Ohtsuka , Tomohide Tanaka , Yukio Narabu
发明人： Akio Imai , Hideaki Watanabe , Takashi Kajima , Yasushi Ishihama , Akiyo Ohtsuka , Tomohide Tanaka , Yukio Narabu
IPC分类号： C07D211/32 , A01N43/40 , A01N43/46 , C07D207/00 , C07D211/20
CPC分类号： C07D211/32
摘要： Donepezil hydrochloride, 1-benzyl-4-[(5,6-dimethoxy-1-indanon)-2-yl]methylpiperidine hydrochloride, is provided here in the form of four polymorphs which are stable against heat and humidity in the pharmaceutical use. They can be industrially produced. They are specified by peaks in X-ray powder diffraction pattern and absorption peaks in infrared absorption spectra in potassium bromide.

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