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11. 发明授权

US06435018B1 Vehicle abnormality diagnosis system 有权
标题翻译：车辆异常诊断系统
公开(公告)号：US06435018B1
公开(公告)日：2002-08-20
申请号：US09553549
申请日：2000-04-20
申请人： Taku Murakami , Ichio Ichikawa , Haruo Hashimoto , Fumihide Satou
发明人： Taku Murakami , Ichio Ichikawa , Haruo Hashimoto , Fumihide Satou
IPC分类号： G01M1500
CPC分类号： G08G1/20 , G01C21/20 , G01M17/007
摘要： Either a specified point Q or a specified area Ar, at which an operating condition of a vehicle becomes a specified operating condition, is set beforehand from among points or areas on a travel route along which a vehicle travels. Then, an operating parameter P of a point in time ts1, ts2, ts3, ts4 . . . , tsn, at which a vehicle passes either a specified point Q or a specified area Ar, is obtained. Then, a vehicle abnormality is diagnosed on the basis of the values of these obtained operating parameters P (maximum values Pmax).
摘要翻译：从车辆行驶的行驶路线上的点或区域中预先设定指定点Q或车辆的运转状态为规定的运转状态的指定区域Ar。然后是时间点ts1，ts2，ts3，ts4的运行参数P. 。。，tsn，其中车辆通过指定点Q或指定区域Ar。然后，基于这些获得的操作参数P（最大值Pmax）的值来诊断车辆异常。

12. 发明授权

US6141629A Method and apparatus for determining machine maintenance due times 失效
标题翻译：用于确定机器维护的时间的方法和装置
公开(公告)号：US6141629A
公开(公告)日：2000-10-31
申请号：US114566
申请日：1998-07-13
申请人： Shigeru Yamamoto , Kunihiko Imanishi , Takao Nagai , Sadachika Akiyama , Jiro Akagi , Nobuki Hasegawa , Kazunori Kuromoto , Taku Murakami
发明人： Shigeru Yamamoto , Kunihiko Imanishi , Takao Nagai , Sadachika Akiyama , Jiro Akagi , Nobuki Hasegawa , Kazunori Kuromoto , Taku Murakami
IPC分类号： E02F9/26 , E02F9/20 , E02F9/24 , G06F17/40
CPC分类号： E02F9/205
摘要： Maintenance due times of components of a machine are determined according to the status of user-performed in-house maintenance and according to the occurrence of abnormalities during machine operation. A subtraction point (20 points) associated with an abnormality (overheating) detected during machine operation is subtracted from the score (80 points) of a corresponding component (engine) in the machine. An addition point (50 points) associated with the type of maintenance (overhaul performed by in-house maintenance) indicated in maintenance information is added to the score (80 points). Thus, at the time where the value resulting from subtractions from and additions to the score for that component (engine) reaches a prescribed value (10 points) indicating maintenance due time, it is determined that the maintenance due time for that component (engine) has been reached.
摘要翻译：机器部件的维护期限根据用户内部维护的状态和机器运行过程中出现的异常情况确定。在机器操作期间检测到的与异常（过热）相关联的减法点（20点）从机器中的对应部件（引擎）的得分（80分）中减去。在维护信息中指示的与维护类型（由内部维护进行的大修）相关联的附加点（50分）被添加到得分（80分）。因此，在从该部件（发动机）的得分的减法和添加得到的值达到指示维护到期时间的规定值（10分）的时刻，确定该部件（引擎）的维护到期时间，已经到达

13. 发明授权

US08916351B2 Primer generation rolling circle amplification 有权
标题翻译：引物生成滚圈放大
公开(公告)号：US08916351B2
公开(公告)日：2014-12-23
申请号：US11813142
申请日：2006-01-04
申请人： Taku Murakami
发明人： Taku Murakami
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12P19/34 , C12Q1/6844 , C12Q2531/125
摘要： A method of amplifying a nucleic acid includes combining a first linear primer with a polymerase and a circular probe. The circular probe contains at least one antisense sequence to a second nucleic acid sequence and at least one antisense sequence to the first linear primer. The method also includes producing at least one repeat of an antisense copy of the circular probe by rolling circle amplification. The antisense copy contains at least the second nucleic acid sequence. The method further includes generating more than one second linear primers from each copy of the second nucleic acid sequence; and hybridizing the second linear primers to more than one of the circular probes. A ribbon probe includes a circular probe and a lock probe. The lock probe contains at least a cleavable linker, and the circular probe and the lock probe are unable to dissociate without cleaving the cleavable linker.
摘要翻译：扩增核酸的方法包括将第一线性引物与聚合酶和圆形探针组合。圆形探针含有至少一个与第二个核酸序列的反义序列和至少一个与第一个线性引物的反义序列。该方法还包括通过滚环扩增产生圆形探针的反义拷贝的至少一个重复。反义拷贝至少含有第二个核酸序列。该方法还包括从第二核酸序列的每个拷贝产生多于一个的第二线性引物; 并将第二线性引物与多于一个的圆形探针杂交。带状探头包括圆形探针和锁定探针。锁定探针至少包含可切割的接头，圆形探针和锁定探针无法解离，而不会切割可切割的接头。

14. 发明申请

US20120282623A1 RAPID PATHOGEN DETECTION TECHNIQUES AND APPARATUS 审中-公开
标题翻译：快速病毒检测技术和设备
公开(公告)号：US20120282623A1
公开(公告)日：2012-11-08
申请号：US13574296
申请日：2011-01-24
申请人： Taku Murakami , Toshit Sen
发明人： Taku Murakami , Toshit Sen
IPC分类号： C12Q1/04 , C12Q1/68 , G01N33/569
CPC分类号： C12Q1/04
摘要： Methods for selectively detecting a live pathogen in a sample containing live and dead pathogens, without detecting the dead pathogen are disclosed. Such methods may include: (i) immobilizing at least a portion of the live and dead pathogens on a solid support with a physical barrier; (ii) incubating the solid support in a growth medium, where the live pathogen can multiply and the multiplied pathogen move from the solid support to a supernatant of the growth medium; and (iii) detecting the multiplied pathogen in the supernatant by a pathogen assay.
摘要翻译：公开了在不检测死亡病原体的情况下选择性检测含有活的和死亡病原体的样品中的活的病原体的方法。这样的方法可以包括：（i）将至少一部分活的和死的病原体固定在具有物理屏障的固体支持物上; （ii）将固体支持物孵育在生长培养基中，其中活病原体可以繁殖并且增殖的病原体从固体支持物移动到生长培养基的上清液; 和（iii）通过病原体测定检测上清液中繁殖的病原体。

15. 发明授权

US06778893B2 Control system for construction machines 有权
标题翻译：施工机械控制系统
公开(公告)号：US06778893B2
公开(公告)日：2004-08-17
申请号：US09951403
申请日：2001-09-14
申请人： Taku Murakami , Megumi Murase , Tadashi Taninaga
发明人： Taku Murakami , Megumi Murase , Tadashi Taninaga
IPC分类号： G06F1900
CPC分类号： G08G1/20
摘要： Abnormality can be judged accurately by considering information other than a sensor-detected value. Determination of an abnormality degree, namely an urgency degree, is enabled, and an abnormality can be processed accurately according to the determined result. Only information really useful is given to the side of an administrator, and the communications cost is suppressed. Server unit 11 of manufacturer 10 judges the abnormality degree of a construction machine on the basis of a combination of all or two of first to third construction machine information D1, D2, D3 collected. The server unit 11 of the manufacturer 10 ranks the abnormality degree of the construction machine in respective levels of Rank 1 (Normal), Rank 2 (Warning), Rank 3 (Abnormal) and Rank 4 (Emergency) on the basis of the number of occurred times of error codes collected for the unit time.
摘要翻译：可以通过考虑不同于传感器检测值的信息来准确判断异常。能够确定异常度即紧急程度，并且可以根据确定的结果精确地处理异常。只有信息真正有用，给管理员一方，通信费用被抑制。制造商10的服务器单元11基于收集的第一到第三施工机器信息D1，D2，D3中的全部或者两个的组合来判断建筑机械的异常度。制造商10的服务器单元11以等级1（正常），等级2（警告），等级3（异常）和等级4（紧急）的各个级别的等级排列施工机械的异常度出现单位时间收集的错误代码的时间。

16. 发明授权

US5773785A Plasma cutting apparatus for concrete structures 失效
标题翻译：混凝土结构用等离子切割装置
公开(公告)号：US5773785A
公开(公告)日：1998-06-30
申请号：US659988
申请日：1996-06-07
申请人： Yuukou Takeuchi , Hitoshi Hayakawa , Taku Murakami , Tooru Fukuda , Akifumi Hoshino
发明人： Yuukou Takeuchi , Hitoshi Hayakawa , Taku Murakami , Tooru Fukuda , Akifumi Hoshino
IPC分类号： B23K10/00 , E21B7/14
CPC分类号： B23K10/00 , E21B7/146
摘要： A compact plasma cutting apparatus suitable for the thermal cutting of concrete structures includes a sealed housing to which a plasma gas is fed under pressure. A metal strip that serves as an outer hollow electrode is bent such that a widthwise section thereof is in circular form, while a metal rod or tube is continuously inserted through a hollow portion of the thus formed outer electrode, with an insulator located between both electrodes. While gas is passed between the inner and outer electrodes, an arc is produced at open ends of both electrodes to generate a plasma gas, which is in turn blown onto a concrete structure for thermal cutting. The formation of both electrodes is effected depending on consumption of the electrodes, thereby carrying out thermal cutting continuously. A high-efficient yet economical cutting method is achieved using the cutting apparatus.
摘要翻译：适用于混凝土结构的热切割的小型等离子切割装置包括密封的壳体，等离子体气体在压力下被供给到该壳体。用作外部中空电极的金属带被弯曲成宽度方向为圆形，而金属棒或管被连续插入穿过如此形成的外部电极的中空部分，绝缘体位于两个电极之间。当气体在内电极和外电极之间通过时，在两个电极的开口端处产生电弧，以产生等离子体气体，然后等离子体气体被吹到用于热切割的混凝土结构上。两电极的形成取决于电极的消耗，从而连续进行热切割。使用切割装置实现了高效且经济的切割方法。

17. 发明授权

US4541304A Connecting rod made of fiber reinforced plastic material 失效
标题翻译：由纤维增强塑料材料制成的连杆
公开(公告)号：US4541304A
公开(公告)日：1985-09-17
申请号：US478772
申请日：1983-03-25
申请人： Kazuo Chikugo , Taku Murakami , Yorihiko Inada
发明人： Kazuo Chikugo , Taku Murakami , Yorihiko Inada
IPC分类号： F16C7/00
CPC分类号： F16C7/026 , Y10T29/49288 , Y10T74/2162
摘要： A connecting rod has at one end thereof two leg portions formed in forked configuration defining therebetween an intermediate groove having a recess of semi-circular cross section on the inside thereof and having oppositely located bolt insertion holes at the leading ends thereof, respectively. In the other side of the intermediate groove is fitted a block having a semi-circular recess defining a crankshaft bearing hole in opposed relationship with said semi-circular recess of the intermediate groove and having a bolt insertion hole formed therethrough and extending coaxially with each of the bolt insertion holes so as to tighten up and fixedly secure the block between the leg portions by a bolt and nut unit.
摘要翻译：连杆在其一端具有两个脚部分，形成为叉形结构，在其间形成有分别具有在其内侧上具有半圆形横截面的凹部的中间槽，并且在其前端处具有相对定位的螺栓插入孔。在中间槽的另一侧装配有具有半圆形凹部的块，该半圆形凹部限定了与中间槽的所述半圆形凹部相对的曲轴轴承孔，并且具有穿过其形成的螺栓插入孔，并且与每个螺栓插入孔通过螺栓和螺母单元紧固并固定在腿部之间。

18. 发明申请

US20120295818A1 METHOD OF MULTI PATHOGEN DETECTION 审中-公开
标题翻译：多病原体检测方法
公开(公告)号：US20120295818A1
公开(公告)日：2012-11-22
申请号：US13574270
申请日：2011-01-24
申请人： Taku Murakami
发明人： Taku Murakami
IPC分类号： C12Q1/04 , C40B30/00 , C12Q1/68 , C40B30/04 , C12N1/20 , G01N33/569
CPC分类号： C12Q1/04
摘要： A culturing method includes culturing a sample having one or more species of target pathogens of a detection assay and competing microorganisms under oxygen-poor condition in a growth medium. The growth medium can be a non-selective medium. The oxygen-poor condition is effective to prevent competing microorganisms from suppressing growth of the target pathogens. This method can be used in combination with a sample preparation method for large volume particulate samples using highly porous filter material and porous spherical filter aid.
摘要翻译：培养方法包括在生长培养基中培养具有检测测定的一种或多种目标病原体的样品和在贫氧条件下的竞争性微生物。生长培养基可以是非选择性培养基。缺氧条件有效地防止竞争性微生物抑制靶病原体的生长。该方法可以与使用高度多孔过滤材料和多孔球形助滤剂的大体积颗粒样品的样品制备方法结合使用。

19. 发明申请

US20110045470A1 PATHOGEN DETECTION IN LARGE-VOLUME PARTICULATE SAMPLES 审中-公开
标题翻译：大容量样品中的病原体检测
公开(公告)号：US20110045470A1
公开(公告)日：2011-02-24
申请号：US12671675
申请日：2008-08-01
申请人： Taku Murakami , Jifan Li , Masato Mitsuhashi , Melanie Oakes
发明人： Taku Murakami , Jifan Li , Masato Mitsuhashi , Melanie Oakes
IPC分类号： C12Q1/68 , C12Q1/04 , C12N1/02 , C12M1/34 , G01N33/53
CPC分类号： C12Q1/04 , C12Q1/24
摘要： Methods and filter systems for detecting microorganisms in a sample, such as a food sample, are disclosed. The filter is configured to attract the microorganism, for example by electrostatic charge. The filter containing the microorganism is incubated to grow the microorganism so that it may be detected. The filter may be degradable and the detection may involve extracting the microorganism or the molecular marker of the microorganism from the filter for detection. The filter system may also include a porous microbead component selected to trap dirt and other contaminants from the sample while allowing the microorganisms to pass among the microbeads. Methods are disclosed for detecting microorganisms in samples using the filter systems described. The methods may also include adding a polymer and/or a microorganism detection reagent to the filter to localize microorganism growth and prevent evaporation of reagents.
摘要翻译：公开了用于检测样品（例如食品样品）中的微生物的方法和过滤系统。过滤器被配置为吸引微生物，例如通过静电电荷。将含有微生物的过滤器温育以生长微生物，使其可以被检测。过滤器可以是可降解的，并且检测可能涉及从过滤器中提取微生物或微生物的分子标记物进行检测。过滤系统还可以包括多孔微珠组分，其被选择用于从样品中捕获污垢和其它污染物，同时允许微生物在微珠之间通过。公开了使用所述过滤系统检测样品中的微生物的方法。所述方法还可以包括向过滤器中加入聚合物和/或微生物检测试剂以定位微生物生长并防止试剂的蒸发。

20. 发明申请

US20090233277A1 PRIMER GENERATION ROLLING CIRCLE AMPLIFICATION 有权
标题翻译：主要产生滚子圆柱放大
公开(公告)号：US20090233277A1
公开(公告)日：2009-09-17
申请号：US11813142
申请日：2006-01-04
申请人： Taku Murakami
发明人： Taku Murakami
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04
CPC分类号： C12P19/34 , C12Q1/6844 , C12Q2531/125
摘要： A method of amplifying a nucleic acid is provided which comprises: generating a first nucleic acid primer from a first nucleic acid sequence; combining the first nucleic acid primer with a first polymerase and a first circular nucleic acid probe, wherein the first circular nucleic acid probe contains at least one antisense sequence to a second nucleic acid sequence and at least one antisense sequence to the first nucleic acid primer; producing at least one repeat of a sequence copy of the first circular nucleic acid probe by rolling circle amplification using the first polymerase, wherein the sequence copy contains at least the second nucleic acid sequence; generating a second nucleic acid primer from the second nucleic acid sequence; combining the second nucleic acid primer with a second polymerase and a second circular nucleic acid probe, where the second circular nucleic acid probe contains at least one antisense sequence to the second nucleic acid primer; and producing at least one repeat of a sequence copy of the second circular nucleic acid probe by rolling circle amplification using the second polymerase. The method may be employed to detect molecules of interest such as nucleic acid sequences, DNA methylation, single nucleotide polymorphisms (SNP), proteins and posttranslational modifications. Furthermore, a ribbon probe is provided that comprises a circular nucleic acid probe and a nucleic acid lock probe, wherein: the nucleic acid lock probe contains at least a cleavable linker, and the circular nucleic acid probe and the lock probe are unable to dissociate without cleaving the cleavable linker.
摘要翻译：提供了扩增核酸的方法，其包括：从第一核酸序列产生第一核酸引物; 将第一核酸引物与第一聚合酶和第一环状核酸探针组合，其中第一环状核酸探针含有至少一个与第二核酸序列的反义序列和至少一个与第一核酸引物的反义序列; 通过使用所述第一聚合酶的滚环扩增产生所述第一环状核酸探针的序列拷贝的至少一个重复序列，其中所述序列拷贝至少含有所述第二核酸序列; 从所述第二核酸序列产生第二核酸引物; 将所述第二核酸引物与第二聚合酶和第二环状核酸探针组合，其中所述第二环状核酸探针含有至少一个与所述第二核酸引物的反义序列; 以及通过使用第二聚合酶的滚环扩增产生第二环状核酸探针的序列拷贝的至少一个重复。该方法可用于检测感兴趣的分子，例如核酸序列，DNA甲基化，单核苷酸多态性（SNP），蛋白质和翻译后修饰。此外，提供了带状探针，其包括圆形核酸探针和核酸锁定探针，其中：所述核酸锁定探针至少含有可切割的接头，并且所述环状核酸探针和所述锁定探针不能在没有切割可切割的接头。

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