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11. 发明专利

AU2021201551A1 Quinone methide analog signal amplification 未知
公开(公告)号：AU2021201551A1
公开(公告)日：2021-04-01
申请号：AU2021201551
申请日：2021-03-11
申请人： VENTANA MED SYST INC
发明人： BIENIARZ CHRISTOPHER , ASHWORTH-SHARPE JULIA , KELLY BRIAN D , POLASKE NATHAN
IPC分类号： C07C27/00 , G01N33/58
摘要： QUINONE METHIDE ANALOG SIGNAL AMPLIFICATION Abstract 110 ~LG ' a _1__-N 1 HN 40 0'0 HN 0 ,. O IG -D aH Disclosed herein are novel quinone methide analog precursors and embodiments of a method and a kit of using the same for detecting one or more targets in a biological sample. The method of detection comprises contacting the sample with a detection probe, then contacting the sample with a labeling conjugate that comprises an enzyme. The enzyme interacts with a quinone methide analog precursor comprising a detectable label, forming a reactive quinone methide analog, which binds to the biological sample proximally to or directly on the target. The detectable label is then detected. In some embodiments, multiple targets can be detected by multiple quinone methide analog precursors interacting with different enzymes without the need for an enzyme deactivation step.

12. 发明专利

CA2631005C MOLECULAR CONJUGATE 未知
公开(公告)号：CA2631005C
公开(公告)日：2017-02-28
申请号：CA2631005
申请日：2006-11-21
申请人： VENTANA MED SYST INC
发明人： BIENIARZ CHRISTOPHER , ASHWORTH-SHARPE JULIA , KERNAG CASEY A , KOSMEDER JEROME W , LEFEVER MARK
IPC分类号： C07K16/00 , C07C323/60 , C07K16/42 , C07K16/44 , G01N33/532
摘要： A method is disclosed for making a conjugate of two molecules using a hydrazide thiol linker. In a particular working embodiment, an Fc-specifÊc antibody- enzyme conjugate is made using the method and demonstrated to provide exceptional staining sensitivity and specificity in immunohistochemical and in situ hybridization assays.

13. 发明专利

AU2011248607B2 Antibody-nanoparticle conjugates and methods for making and using such conjugates 未知
公开(公告)号：AU2011248607B2
公开(公告)日：2015-02-05
申请号：AU2011248607
申请日：2011-04-27
申请人： VENTANA MED SYST INC
发明人： ASHWORTH-SHARPE JULIA , YUN CHOL STEVEN , ZHILINA ZHANNA , MURILLO ADRIAN E , JOHNSON DONALD D , FARRELL MICHAEL , KOSMEDER JEROME W , BIENIARZ CHRISTOPHER
IPC分类号： G01N33/68 , C07F1/00 , C07F9/12 , C07F9/50 , C07F9/572 , C07F9/655 , G01N33/531 , G01N33/543 , G01N33/58
摘要： Disclosed herein are antibody-nanoparticle conjugates that include two or more nanoparticles (such as gold, palladium, platinum, silver, copper, nickel, cobalt, iridium, or an alloy of two or more thereof) directly linked to an antibody or fragment thereof through a metal-thiol bond. Methods of making the antibody- nanoparticle conjugates disclosed herein include reacting an arylphosphine-nanoparticle composite with a reduced antibody to produce an antibody-nanoparticle conjugate. Also disclosed herein are methods for detecting a target molecule in a sample that include using an antibody-nanoparticle conjugate (such as the antibody- nanoparticle conjugates described herein) and kits for detecting target molecules utilizing the methods disclosed herein.

14. 发明专利

CA2609702A1 ANTIBODY CONJUGATES 未知
公开(公告)号：CA2609702A1
公开(公告)日：2006-11-02
申请号：CA2609702
申请日：2006-04-27
申请人： VENTANA MED SYST INC
发明人： BIENIARZ CHRISTOPHER , ASHWORTH-SHARPE JULIA , WONG JENNIFER , KERNAG CASEY A , LEFEVER MARK , KOSMEDER JEROME W
IPC分类号： A61K47/48
摘要： The present invention relates to antibody-signal generating moiety conjugates, wherein the antibody is covalently linked to the signal generating moiety through a heterobifunctional polyethylene glycol (PEG) linker.

15. 发明专利

CA2824355C APPLICATION OF QUANTUM DOTS FOR NUCLEAR STAINING 未知
公开(公告)号：CA2824355C
公开(公告)日：2020-03-31
申请号：CA2824355
申请日：2012-02-27
申请人： VENTANA MED SYST INC
发明人： YUN CHOL S , KELLY BRIAN D , ASHWORTH-SHARPE JULIA , BIENIARZ CHRISTOPHER , BAMFORD PASCAL , MURILLO ADRIAN E
IPC分类号： C12Q1/68 , B82Y15/00 , C12Q1/6813 , C12Q1/6816 , G01N33/53
摘要： Embodiments of a system, method, and kit for visualizing a nucleus are disclosed. A tissue sample is pretreated with a protease to permeabilize the nucleus, and then incubated with a nanoparticle/DNA-binding moiety conjugate. The DNA-binding moiety includes at least one DNA-binding molecule. The conjugate binds to DNA within the nucleus, and the nanoparticle is visualized, thereby visualizing the nucleus. Computer and image analysis techniques are used to evaluate nuclear features such as chromosomal distribution, ploidy, shape, size, texture features, and/or contextual features. The method may be used in combination with other multiplexed tests on the tissue sample, including fluorescence in situ hybridization. Kits for performing the method include a protease enzyme composition, a nanoparticle/DNA-binding moiety conjugate, and a reaction buffer.

16. 发明专利

DK3111224T3 Quinonmethidanalog-signalforstærkning 未知
公开(公告)号：DK3111224T3
公开(公告)日：2019-03-18
申请号：DK15706439
申请日：2015-02-20
申请人： VENTANA MED SYST INC
发明人： BIENIARZ CHRISTOPHER , KELLY BRIAN D , POLASKE NATHAN , ASHWORTH-SHARPE JULIA
IPC分类号： G01N33/58 , C07C27/00

17. 发明专利

CA2792569C ANTIBODY-NANOPARTICLE CONJUGATES AND METHODS FOR MAKING AND USING SUCH CONJUGATES 未知
公开(公告)号：CA2792569C
公开(公告)日：2019-01-15
申请号：CA2792569
申请日：2011-04-27
申请人： VENTANA MED SYST INC
发明人： ASHWORTH-SHARPE JULIA , YUN CHOL STEVEN , ZHILINA ZHANNA , MURILLO ADRIAN E , JOHNSON DONALD D , FARRELL MICHAEL , KOSMEDER JEROME W , BIENIARZ CHRISTOPHER
IPC分类号： G01N33/68 , C07F1/00 , C07F9/12 , C07F9/50 , C07F9/572 , C07F9/655 , G01N33/543 , G01N33/58
摘要： Disclosed herein are antibody-nanoparticle conjugates that include two or more nanoparticles (such as gold, palladium, platinum, silver, copper, nickel, cobalt, iridium, or an alloy of two or more thereof) directly linked to an antibody or fragment thereof through a metal-thiol bond. Methods of making the antibody- nanoparticle conjugates disclosed herein include reacting an arylphosphine-nanoparticle composite with a reduced antibody to produce an antibody-nanoparticle conjugate. Also disclosed herein are methods for detecting a target molecule in a sample that include using an antibody-nanoparticle conjugate (such as the antibody- nanoparticle conjugates described herein) and kits for detecting target molecules utilizing the methods disclosed herein.

18. 发明专利

DK2963011T3 MOLEKYLÆRT KONJUGAT 未知
公开(公告)号：DK2963011T3
公开(公告)日：2018-08-06
申请号：DK15001958
申请日：2006-11-21
申请人： VENTANA MED SYST INC
发明人： BIENIARZ CHRISTOPHER , ASHWORTH-SHARPE JULIA , KERNAG CASEY A , KOSMEDER JEROME W , LEFEVER MARK
IPC分类号： C07C319/02 , A61K47/68 , C07C323/60 , C07C327/28 , C12N9/16 , C12N9/96 , G01N33/532

19. 发明专利

ES2677555T3 Conjugado molecular 未知
公开(公告)号：ES2677555T3
公开(公告)日：2018-08-03
申请号：ES15001958
申请日：2006-11-21
申请人： VENTANA MED SYST INC
发明人： BIENIARZ CHRISTOPHER , ASHWORTH-SHARPE JULIA , KERNAG CASEY A , KOSMEDER JEROME W , LEFEVER MARK
IPC分类号： C07C319/02 , A61K47/68 , C07C323/60 , C07C327/28 , C12N9/16 , C12N9/96 , G01N33/532
摘要： Conjugado, que comprende: un anticuerpo, un marcaje detectable que comprende un enzima que incluye aproximadamente 17 a 25 grupos maleimida introducidos en el enzima mediante conectores NHS-PEG-maleimida, encontrándose cada conector unido covalentemente a un grupo amino del enzima, y una pluralidad de conectores hidrazida-tiol, comprendiendo cada conector hidrazida-tiol uno o más átomos de unión situados entre un grupo hidrazida y un grupo tiol, en el que el conector hidrazida-tiol se selecciona de entre hidrazida de ácido mercaptobutírico (MBH), carbohidrazida de ácido mercaptobutírico (MBCH), hidrazida de mecaptoacetamido-ácido mercaptobutírico (MAMBH), hidrazida de ácido tiohexanamidomercaptobutírico (THMBH), N,N'-(6-hidrazinil-6-oxohexano-1,5-diil)bis(2- mercaptoacetamida) (BTAL), bistiohexanamidohidrazidolisina (BTHL), N-(1,5-dihidrazinil-1,5- dioxopentán-2-il)-2-mercapto-acetamida (TAGD), dihidrazida de ácido tiohexamidoglutámico (THGD), un conector hidrazida-tiol basado en PEG, un conector hidrazida-tiol multifuncional, un conector hidrazidatiol multifuncional basado en PEG, un conector hidrazida-tiol de poliacrilamida y combinaciones de los mismos, el grupo hidrazida de cada conector hidrazida-tiol está unido covalentemente a una parte Fc glucosilado oxidado del anticuerpo y el grupo tiol de uno de los conectores hidrazida-tiol unidos covalentemente al marcaje detectable mediante uno de los grupos maleimida.

20. 发明专利

AU2011248607A1 Antibody-nanoparticle conjugates and methods for making and using such conjugates 未知
公开(公告)号：AU2011248607A1
公开(公告)日：2012-09-20
申请号：AU2011248607
申请日：2011-04-27
申请人： VENTANA MED SYST INC
发明人： ASHWORTH-SHARPE JULIA , YUN CHOL STEVEN , ZHILINA ZHANNA , MURILLO ADRIAN E , JOHNSON DONALD D , FARRELL MICHAEL , KOSMEDER JEROME W , BIENIARZ CHRISTOPHER
IPC分类号： G01N33/531 , C07F1/00 , C07F9/12 , C07F9/50 , C07F9/572 , C07F9/655 , G01N33/543 , G01N33/58 , G01N33/68
摘要： Disclosed herein are antibody-nanoparticle conjugates that include two or more nanoparticles (such as gold, palladium, platinum, silver, copper, nickel, cobalt, iridium, or an alloy of two or more thereof) directly linked to an antibody or fragment thereof through a metal-thiol bond. Methods of making the antibody- nanoparticle conjugates disclosed herein include reacting an arylphosphine-nanoparticle composite with a reduced antibody to produce an antibody-nanoparticle conjugate. Also disclosed herein are methods for detecting a target molecule in a sample that include using an antibody-nanoparticle conjugate (such as the antibody- nanoparticle conjugates described herein) and kits for detecting target molecules utilizing the methods disclosed herein.

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