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11. 发明申请

US20110183392A1 YEAST ORGANISM PRODUCING ISOBUTANOL AT A HIGH YIELD 审中-公开
公开(公告)号：US20110183392A1
公开(公告)日：2011-07-28
申请号：US12696645
申请日：2010-01-29
申请人： Reid M. Renny Feldman , Uvini Gunawardena , Jun Urano , Peter Meinhold , Aristos Aristidou , Catherine Asleson Dundon , Christopher Smith
发明人： Reid M. Renny Feldman , Uvini Gunawardena , Jun Urano , Peter Meinhold , Aristos Aristidou , Catherine Asleson Dundon , Christopher Smith
IPC分类号： C12P7/16 , C12N1/19
CPC分类号： C12N9/88 , C12P7/16 , C12Y401/01001 , Y02E50/10
摘要： There is disclosed a method of producing isobutanol. In an embodiment, the method includes providing a microorganism transformed with an isobutanol producing pathway containing at least one exogenous gene. The microorganism is selected to produce isobutanol from a carbon source at a yield of at least 10 percent theoretical. The method includes cultivating the microorganism in a culture medium containing a feedstock providing the carbon source, until isobutanol is produced. The method includes recovering the isobutanol. In one embodiment, the microorganism is a yeast with a Crabtree-negative phenotype. In another embodiment, the microorganism is a yeast microorganism with a Crabtree-positive phenotype. There is disclosed a microorganism for producing isobutanol. In an embodiment, the microorganism includes an isobutanol producing pathway containing at least one exogenous gene, and is selected to produce a recoverable quantity of isobutanol from a carbon source at a yield of at least 10 percent theoretical.

12. 发明申请

US20120028323A1 YEAST ORGANISM PRODUCING ISOBUTANOL AT A HIGH YIELD 审中-公开
标题翻译： YEAST ORGANISM在高产量下生产ISOBUTANOL
公开(公告)号：US20120028323A1
公开(公告)日：2012-02-02
申请号：US13267771
申请日：2011-10-06
申请人： Reid M. Renny Feldman , Uvini Gunawardena , Jun Urano , Peter Meinhold , Aristos Aristidou , Catherine Asleson Dundon , Christopher Smith
发明人： Reid M. Renny Feldman , Uvini Gunawardena , Jun Urano , Peter Meinhold , Aristos Aristidou , Catherine Asleson Dundon , Christopher Smith
IPC分类号： C12P7/16 , C12N1/19
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/0008 , C12N9/1022 , C12N9/88 , C12N9/90 , C12N15/81 , C12Y101/01001 , C12Y101/01008 , C12Y101/01086 , C12Y101/05003 , C12Y202/01006 , C12Y401/01001 , C12Y401/01074 , C12Y402/01009 , Y02E50/10
摘要： The present invention provides recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise a modification resulting in the reduction of pyruvate decarboxylase and/or glycerol-3-phosphate dehydrogenase activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
摘要翻译：本发明提供包含产生异丁醇的代谢途径的重组微生物和使用所述重组微生物产生异丁醇的方法。在本发明的各个方面，重组微生物可以包含导致丙酮酸脱羧酶和/或甘油-3-磷酸脱氢酶活性降低的修饰。在本文所述的各种实施方案中，重组微生物可以是酵母进化枝，Crabtree阴性酵母微生物，Crabtree阳性酵母微生物，WGD（全基因组复制）酵母微生物，WGD前（全基因组复制）酵母微生物，和非发酵酵母微生物。

13. 发明申请

US20110020889A1 YEAST ORGANISM PRODUCING ISOBUTANOL AT A HIGH YIELD 有权
标题翻译： YEAST ORGANISM在高产量下生产ISOBUTANOL
公开(公告)号：US20110020889A1
公开(公告)日：2011-01-27
申请号：US12820505
申请日：2010-06-22
申请人： Reid M. Renny Feldman , Uvini Gunawardena , Jun Urano , Peter Meinhold , Aristos Aristidou , Catherine Asleson Dundon , Christopher Smith
发明人： Reid M. Renny Feldman , Uvini Gunawardena , Jun Urano , Peter Meinhold , Aristos Aristidou , Catherine Asleson Dundon , Christopher Smith
IPC分类号： C12P7/16 , C12N1/19
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/0008 , C12N9/1022 , C12N9/88 , C12N9/90 , C12N15/81 , C12Y101/01001 , C12Y101/01008 , C12Y101/01086 , C12Y101/05003 , C12Y202/01006 , C12Y401/01001 , C12Y401/01074 , C12Y402/01009 , Y02E50/10
摘要： The present invention provides recombinant mircoorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise a modification resulting in the reduction of pyruvate decarboxylase and/or glycerol-3-phosphate dehydrogenase activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
摘要翻译：本发明提供包含产生异丁醇的代谢途径的重组微生物和使用所述重组微生物产生异丁醇的方法。在本发明的各个方面，重组微生物可以包含导致丙酮酸脱羧酶和/或甘油-3-磷酸脱氢酶活性降低的修饰。在本文所述的各种实施方案中，重组微生物可以是酵母进化枝，Crabtree阴性酵母微生物，Crabtree阳性酵母微生物，WGD（全基因组复制）酵母微生物，WGD前（全基因组复制）酵母微生物，和非发酵酵母微生物。

14. 发明申请

US20110318799A1 YEAST ORGANISM PRODUCING ISOBUTANOL AT A HIGH YIELD 审中-公开
标题翻译： YEAST ORGANISM在高产量下生产ISOBUTANOL
公开(公告)号：US20110318799A1
公开(公告)日：2011-12-29
申请号：US13229438
申请日：2011-09-09
申请人： Reid M. Renny Feldman , Uvini Gunawardena , Jun Urano , Peter Meinhold , Aristos A. Aristidou , Catherine Asleson Dundon , Christopher Smith
发明人： Reid M. Renny Feldman , Uvini Gunawardena , Jun Urano , Peter Meinhold , Aristos A. Aristidou , Catherine Asleson Dundon , Christopher Smith
IPC分类号： C12P7/16
CPC分类号： C12N9/88 , C12P7/16 , C12Y401/01001 , Y02E50/10
摘要： There is disclosed a method of producing isobutanol. In an embodiment, the method includes providing a microorganism transformed with an isobutanol producing pathway containing at least one exogenous gene. The microorganism is selected to produce isobutanol from a carbon source at a yield of at least 10 percent theoretical. The method includes cultivating the microorganism in a culture medium containing a feedstock providing the carbon source, until isobutanol is produced. The method includes recovering the isobutanol. In one embodiment, the microorganism is a yeast with a Crabtree-negative phenotype. In another embodiment, the microorganism is a yeast microorganism with a Crabtree-positive phenotype. There is disclosed a microorganism for producing isobutanol. In an embodiment, the microorganism includes an isobutanol producing pathway containing at least one exogenous gene, and is selected to produce a recoverable quantity of isobutanol from a carbon source at a yield of at least 10 percent theoretical.
摘要翻译：公开了异丁醇的制造方法。在一个实施方案中，该方法包括提供转化了含有至少一种外源基因的产生异丁醇的途径的微生物。选择微生物以产生至少10％的理论值从碳源产生异丁醇。该方法包括在含有提供碳源的原料的培养基中培养微生物，直到产生异丁醇。该方法包括回收异丁醇。在一个实施方案中，微生物是具有Crabtree-阴性表型的酵母。在另一个实施方案中，微生物是具有Crabtree阳性表型的酵母微生物。公开了用于生产异丁醇的微生物。在一个实施方案中，微生物包括含有至少一种外源基因的异丁醇生产途径，并且选择其以至少10％理论值的产率从碳源产生可回收量的异丁醇。

15. 发明授权

US08017375B2 Yeast organism producing isobutanol at a high yield 无效
标题翻译：以高产率生产异丁醇的酵母生物
公开(公告)号：US08017375B2
公开(公告)日：2011-09-13
申请号：US12343375
申请日：2008-12-23
申请人： Reid M. Renny Feldman , Uvini Gunawardena , Jun Urano , Peter Meinhold , Aristos A. Aristidou , Catherine Asleson Dundon , Christopher Smith
发明人： Reid M. Renny Feldman , Uvini Gunawardena , Jun Urano , Peter Meinhold , Aristos A. Aristidou , Catherine Asleson Dundon , Christopher Smith
IPC分类号： C12P7/04 , C12P7/16 , C12N1/15 , C12N1/16 , C12N15/00 , C12N9/88
CPC分类号： C12N9/88 , C12P7/16 , C12Y401/01001 , Y02E50/10
摘要： There is disclosed a method of producing isobutanol. In an embodiment, the method includes providing a microorganism transformed with an isobutanol producing pathway containing at least one exogenous gene. The microorganism is selected to produce isobutanol from a carbon source at a yield of at least 10 percent theoretical. The method includes cultivating the microorganism in a culture medium containing a feedstock providing the carbon source, until isobutanol is produced. The method includes recovering the isobutanol. In one embodiment, the microorganism is a yeast with a Crabtree-negative phenotype. In another embodiment, the microorganism is a yeast microorganism with a Crabtree-positive phenotype. There is disclosed a microorganism for producing isobutanol. In an embodiment, the microorganism includes an isobutanol producing pathway containing at least one exogenous gene, and is selected to produce a recoverable quantity of isobutanol from a carbon source at a yield of at least 10 percent theoretical.
摘要翻译：公开了异丁醇的制造方法。在一个实施方案中，该方法包括提供转化了含有至少一种外源基因的产生异丁醇的途径的微生物。选择微生物以产生至少10％的理论值从碳源产生异丁醇。该方法包括在含有提供碳源的原料的培养基中培养微生物，直到产生异丁醇。该方法包括回收异丁醇。在一个实施方案中，微生物是具有Crabtree-阴性表型的酵母。在另一个实施方案中，微生物是具有Crabtree阳性表型的酵母微生物。公开了用于生产异丁醇的微生物。在一个实施方案中，微生物包括含有至少一种外源基因的异丁醇生产途径，并且选择其以至少10％理论值的产率从碳源产生可回收量的异丁醇。

16. 发明申请

US20120028324A1 ENGINEERED MICROORGANISMS CAPABLE OF PRODUCING TARGET COMPOUNDS UNDER ANAEROBIC CONDITIONS 有权
标题翻译：在无机条件下生产目标化合物的工程微生物
公开(公告)号：US20120028324A1
公开(公告)日：2012-02-02
申请号：US13269937
申请日：2011-10-10
申请人： Thomas Buelter , Peter Meinhold , Reid M. Renny Feldman , Andrew Hawkins , Sabine Bastian , Frances Arnold , Jun Urano
发明人： Thomas Buelter , Peter Meinhold , Reid M. Renny Feldman , Andrew Hawkins , Sabine Bastian , Frances Arnold , Jun Urano
IPC分类号： C12P7/16 , C12N1/00 , C12N1/19 , C07H21/04
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/0008 , C12N9/0036 , Y02E50/10 , Y02E50/17
摘要： The present invention is generlly provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention.
摘要翻译：本发明总体上提供了重组微生物，其包含能够在有氧和厌氧条件下产生C3-C5醇的工程化代谢途径。本发明还提供已被突变或修饰以提高其NADH依赖性活性或将辅因子偏好从NADPH转变为NADH并在修饰的微生物中表达的酮酸酸还原异构酶。此外，本发明提供了以修饰微生物表达的异丁醛脱氢酶。还提供了在需氧和厌氧条件下通过使合适的底物与本发明的改性微生物接触来生产有益代谢物的方法。

17. 发明申请

US20120058532A1 ENGINEERED MICROORGANISMS CAPABLE OF PRODUCING TARGET COMPOUNDS UNDER ANAEROBIC CONDITIONS 审中-公开
标题翻译：在无机条件下生产目标化合物的工程微生物
公开(公告)号：US20120058532A1
公开(公告)日：2012-03-08
申请号：US13271084
申请日：2011-10-11
申请人： Thomas Buelter , Peter Meinhold , Reid M. Renny Feldman , Andrew Hawkins , Sabine Bastian , Frances Arnold , Jun Urano
发明人： Thomas Buelter , Peter Meinhold , Reid M. Renny Feldman , Andrew Hawkins , Sabine Bastian , Frances Arnold , Jun Urano
IPC分类号： C12P7/16 , C12N1/19 , C12N15/53
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/0008 , C12N9/0036 , Y02E50/10 , Y02E50/17
摘要： The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention.
摘要翻译：本发明通常提供重组微生物，其包含能够在需氧和厌氧条件下生产C3-C5醇的工程化代谢途径。本发明还提供已被突变或修饰以提高其NADH依赖性活性或将辅因子偏好从NADPH转变为NADH并在修饰的微生物中表达的酮酸酸还原异构酶。此外，本发明提供了以修饰微生物表达的异丁醛脱氢酶。还提供了在需氧和厌氧条件下通过使合适的底物与本发明的改性微生物接触来生产有益代谢物的方法。

18. 发明申请

US20120190089A1 ENGINEERED MICROOGRANISMS CAPABLE OF PRODUCING TARGET COMPOUNDS UNDER ANAEROBIC CONDITIONS 审中-公开
标题翻译：在无机条件下生产目标化合物的工程化微生物
公开(公告)号：US20120190089A1
公开(公告)日：2012-07-26
申请号：US13303884
申请日：2011-11-23
申请人： Thomas Buelter , Peter Meinhold , Reid M. Renny Feldman , Andrew C. Hawkins , Sabine Bastian , Frances Arnold , Jun Urano
发明人： Thomas Buelter , Peter Meinhold , Reid M. Renny Feldman , Andrew C. Hawkins , Sabine Bastian , Frances Arnold , Jun Urano
IPC分类号： C12P7/16 , C12N1/19
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/0008 , C12N9/0036 , Y02E50/10
摘要： The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention.
摘要翻译：本发明通常提供重组微生物，其包含能够在需氧和厌氧条件下生产C3-C5醇的工程化代谢途径。本发明还提供已被突变或修饰以提高其NADH依赖性活性或将辅因子偏好从NADPH转变为NADH并在修饰的微生物中表达的酮酸酸还原异构酶。此外，本发明提供以修饰微生物表达的异丁醛脱氢酶。还提供了在需氧和厌氧条件下通过使合适的底物与本发明的改性微生物接触来生产有益代谢物的方法。

19. 发明授权

US08097440B1 Engineered microorganisms capable of producing target compounds under anaerobic conditions 有权
标题翻译：能够在厌氧条件下产生目标化合物的工程微生物
公开(公告)号：US08097440B1
公开(公告)日：2012-01-17
申请号：US13269937
申请日：2011-10-10
申请人： Thomas Buelter , Peter Meinhold , Reid M. Renny Feldman , Andrew C. Hawkins , Jun Urano , Sabine Bastian , Frances Arnold
发明人： Thomas Buelter , Peter Meinhold , Reid M. Renny Feldman , Andrew C. Hawkins , Jun Urano , Sabine Bastian , Frances Arnold
IPC分类号： C12P7/16 , C12N15/53 , C12N1/15 , C12N9/02
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/0008 , C12N9/0036 , Y02E50/10 , Y02E50/17
摘要： The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention.
摘要翻译：本发明通常提供重组微生物，其包含能够在需氧和厌氧条件下生产C3-C5醇的工程化代谢途径。本发明还提供已被突变或修饰以提高其NADH依赖性活性或将辅因子偏好从NADPH转变为NADH并在修饰的微生物中表达的酮酸酸还原异构酶。此外，本发明提供以修饰微生物表达的异丁醛脱氢酶。还提供了在需氧和厌氧条件下通过使合适的底物与本发明的改性微生物接触来生产有益代谢物的方法。

20. 发明授权

US08232089B2 Cytosolic isobutanol pathway localization for the production of isobutanol 有权
标题翻译：用于生产异丁醇的胞质异丁醇途径定位
公开(公告)号：US08232089B2
公开(公告)日：2012-07-31
申请号：US12855276
申请日：2010-08-12
申请人： Jun Urano , Catherine Asleson Dundon
发明人： Jun Urano , Catherine Asleson Dundon
IPC分类号： C12N1/00 , C07H21/02 , C12P7/16
CPC分类号： C12N15/81 , C12N9/0006 , C12N9/1022 , C12N9/88 , C12P7/16 , Y02E50/10
摘要： The present invention provides recombinant microorganisms comprising isobutanol producing metabolic pathway with at least one isobutanol pathway enzyme localized in the cytosol, wherein said recombinant microorganism is selected to produce isobutanol from a carbon source. Methods of using said recombinant microorganisms to produce isobutanol are also provided. In various aspects of the invention, the recombinant microorganisms may comprise a cytosolically active isobutanol pathway enzymes. In some embodiments, the invention provides mutated, modified, and/or chimeric isobutanol pathway enzymes with cytosolic activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
摘要翻译：本发明提供重组微生物，其包含产生异丁醇的代谢途径与位于细胞溶质中的至少一种异丁醇途径酶，其中所述重组微生物被选择以从碳源产生异丁醇。还提供了使用所述重组微生物产生异丁醇的方法。在本发明的各个方面，重组微生物可以包含胞质活性异丁醇途径酶。在一些实施方案中，本发明提供具有胞质活性的突变，修饰和/或嵌合异丁醇途径酶。在本文所述的各种实施方案中，重组微生物可以是酵母进化枝，Crabtree阴性酵母微生物，Crabtree阳性酵母微生物，WGD（全基因组复制）酵母微生物，WGD前（全基因组复制）酵母微生物，和非发酵酵母微生物。

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