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11. 发明申请

US20120196765A1 METHOD FOR DETECTION OR ANALYSIS OF TARGET SEQUENCE IN GENOMIC DNA 审中-公开
标题翻译：用于检测或分析基因组DNA中目标序列的方法
公开(公告)号：US20120196765A1
公开(公告)日：2012-08-02
申请号：US13382594
申请日：2010-07-09
申请人： Mitsuo Kawase , Toshikazu Hirota , Kousuke Niwa
发明人： Mitsuo Kawase , Toshikazu Hirota , Kousuke Niwa
IPC分类号： C40B30/04 , C40B40/06
CPC分类号： C12Q1/6827 , C12Q1/6837 , C12Q2565/519 , C12Q2561/125
摘要： A method of detecting or analyzing a target sequence in a genomic DNA by using a capture probe immobilized on a solid carrier includes: bringing the target nucleic acid into contact with a first query probe that has a sequence complementary to a portion of the target sequence or to a sequence adjacent to the portion and a second query probe that has a sequence complementary to another portion of the target sequence or to a sequence adjacent to the another portion and that has a recognition sequence complementary to a portion of the capture probe; acquiring a ligated molecule by ligating the first query probe and the second query probe that are hybridized to the target nucleic acid; bringing the ligated molecule into contact with the capture probe on the solid carrier and then capturing the ligated molecule on the solid carrier by hybridizing the capture probe with the recognition sequence in the ligated molecule; and detecting the captured ligated molecule.
摘要翻译：通过使用固定在固体载体上的捕获探针来检测或分析基因组DNA中的靶序列的方法包括：使靶核酸与具有与靶序列的一部分互补的序列的第一查询探针接触，或与所述部分相邻的序列和第二查询探针，其具有与所述靶序列的另一部分互补的序列或与所述另一部分相邻的序列并具有与所述捕获探针的一部分互补的识别序列; 通过连接与靶核酸杂交的第一查询探针和第二查询探针来获得连接的分子; 使连接的分子与固体载体上的捕获探针接触，然后通过将捕获探针与连接的分子中的识别序列杂交捕获固定载体上的连接分子; 并检测捕获的结扎分子。

12. 发明授权

US07052842B2 Method for highly sensitive hybridization of nucleic acids, and method for gene analysis using the same 失效
标题翻译：核酸高度杂交的方法，以及使用其的基因分析方法
公开(公告)号：US07052842B2
公开(公告)日：2006-05-30
申请号：US10258344
申请日：2002-03-01
申请人： Mitsuo Kawase , Kazunari Yamada , Isao Miyazaki , Chiho Matsuda
发明人： Mitsuo Kawase , Kazunari Yamada , Isao Miyazaki , Chiho Matsuda
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6827 , C12Q2521/301 , C12Q2527/101
摘要： A method for hybridizing nucleic acids, which includes an annealing step of preparing a first single stranded nucleic acid fragment immobilized on a surface of an immobilizing material and a second single stranded nucleic acid fragment labeled with fluorescence or radioisotope and forming a complementary double strand from the first single stranded nucleic acid fragment and the second single stranded nucleic acid fragment, and an enzyme treatment step. In the annealing step, the complementary double strand is formed by performing a temperature gradient processing performed from a high temperature area to a low temperature area, and in the enzyme treatment step, a noncomplementary nucleic acid portion contained in the complementary double stand is recognized and cleaved by adding endonuclease. This method has high measurement sensitivity and the operation is simple.
摘要翻译：一种杂交核酸的方法，其包括制备固定在固定化材料表面上的第一单链核酸片段的退火步骤和用荧光或放射性同位素标记的第二单链核酸片段，并形成互补双链第一单链核酸片段和第二单链核酸片段，以及酶处理步骤。在退火工序中，通过进行从高温区域到低温区域的温度梯度处理而形成互补双股，在酶处理工序中，识别出互补双支架中包含的非互补核酸部分，通过添加内切核酸酶切割。该方法测量灵敏度高，操作简单。

13. 发明授权

US06182659B2 Virus-removing filter 失效
标题翻译：病毒去除过滤器
公开(公告)号：US06182659B2
公开(公告)日：2001-02-06
申请号：US09093790
申请日：1998-06-09
申请人： Mitsuo Kawase , Yuji Kawase , Kazunari Yamada , Yasuo Suzuki
发明人： Mitsuo Kawase , Yuji Kawase , Kazunari Yamada , Yasuo Suzuki
IPC分类号： A62B1802
CPC分类号： B01D39/16 , A61L9/16 , B01D2239/0471 , B01J20/22
摘要： A virus-removing filter using, as a virus-capturing body, at least one kind of Streptococcus agalactiae type II culture and Type II polysaccharide.
摘要翻译：使用至少一种无乳链球菌II型培养物和II型多糖作为病毒捕捉体的病毒除去过滤器。

14. 发明申请

US20120178648A1 METHOD FOR DETECTION OF TARGET NUCLEIC ACID 有权
标题翻译：检测目标核酸的方法
公开(公告)号：US20120178648A1
公开(公告)日：2012-07-12
申请号：US13497000
申请日：2010-10-26
申请人： Mitsuo Kawase , Toshikazu Hirota , Kousuke Niwa
发明人： Mitsuo Kawase , Toshikazu Hirota , Kousuke Niwa
IPC分类号： C40B30/04 , C40B40/06
CPC分类号： C12Q1/689 , C12Q1/6858 , C12Q2600/158 , C12Q2531/107 , C12Q2537/143 , C12Q2563/107 , C12Q2565/514
摘要： An object of the disclosure of the present specification is to provide a method for detection of a target nucleic acid which allows construction of an effective detection system of a target nucleic acid. For this purpose, in the disclosure of the present specification, a first primer comprising an identification sequence complementary to a target sequence in a target nucleic acid and a tag addition sequence, and a second primer having a label are prepared. The first primer and the second primer are used for the target nucleic acid in a sample to amplify a chimeric DNA having a tag sequence and the label. The chimeric DNA is hybridized with a detection probe on a solid phase to obtain signal intensity information based on the label, and the target nucleic acid is detected based on the signal intensity information.
摘要翻译：本说明书的公开内容的一个目的是提供一种检测目标核酸的方法，其允许构建靶核酸的有效检测系统。为此，在本说明书的公开内容中，制备包含与靶核酸中的靶序列互补的鉴定序列和标签添加序列的第一引物和具有标记的第二引物。将第一引物和第二引物用于样品中的靶核酸以扩增具有标签序列和标记的嵌合DNA。嵌合DNA与固相上的检测探针杂交，得到基于标记的信号强度信息，根据信号强度信息检测靶核酸。

15. 发明授权

US06395717B1 Therapeutic drug for endotoxin blood symptom and multi-organ failure induced thereby 失效
标题翻译：由此引起内毒素血症和多器官功能衰竭的治疗药物
公开(公告)号：US06395717B1
公开(公告)日：2002-05-28
申请号：US08776032
申请日：1997-01-23
申请人： Michio Ohta , Takaaki Hasegawa , Masayuki Nadai , Yasuko Yoshida , Mitsuo Kawase , Tadahiko Inukai
发明人： Michio Ohta , Takaaki Hasegawa , Masayuki Nadai , Yasuko Yoshida , Mitsuo Kawase , Tadahiko Inukai
IPC分类号： A61K31726
CPC分类号： A61K31/726 , A61K31/7012 , A61K31/715 , Y10S514/921
摘要： Therapeutic drugs for endotoxin blood symptom and multi-organ failure induced by it is provided, which therapeutic during are composed of sialic acid its salt, polymers of sialic acid or a salt of the polymer as effective components and have high therapeutic effects for shock death and organ failure induced by endotoxin as well as high safety, and are effective for treatment of endotoxin-shock, and organ failure.
摘要翻译：提供了由其引起的内毒素血症和多器官功能衰竭的治疗药物，其由唾液酸盐，唾液酸聚合物或聚合物的盐作为有效成分组成，对休克死亡具有很高的治疗效果，内毒素引起的器官功能衰竭以及安全性高，对内毒素休克和器官功能衰竭有效。

16. 发明授权

US5851395A Virus-removing filter 失效
标题翻译：病毒去除过滤器
公开(公告)号：US5851395A
公开(公告)日：1998-12-22
申请号：US771762
申请日：1996-12-20
申请人： Mitsuo Kawase , Yuji Kawase , Kazunari Yamada , Kazukiyo Kobayashi , Yasuo Suzuki
发明人： Mitsuo Kawase , Yuji Kawase , Kazunari Yamada , Kazukiyo Kobayashi , Yasuo Suzuki
IPC分类号： A62B23/02 , A61L9/16 , B01D39/14 , B01J20/26 , C07H5/06 , B01D39/00
CPC分类号： C07H5/06 , A61L9/16 , B01J20/26
摘要： A virus-removing filter using, as a virus-capturing body, at least one kind of sialic acid, a sialic acid derivative, and sugars, glycoproteins and glycolipids containing sialic acid and/or the sialic acid derivative.
摘要翻译：使用作为病毒捕捉体的至少一种唾液酸，唾液酸衍生物和糖，含有唾液酸和/或唾液酸衍生物的糖蛋白和糖脂的病毒除去过滤器。

17. 发明授权

US4257623A Supporting structure for a suspension member for a vehicle 失效
标题翻译：用于车辆的悬挂构件的支撑结构
公开(公告)号：US4257623A
公开(公告)日：1981-03-24
申请号：US41881
申请日：1979-05-23
申请人： Mitsuo Kawase , Hiroyuki Watanabe
发明人： Mitsuo Kawase , Hiroyuki Watanabe
IPC分类号： B60G7/00 , B60G7/02
CPC分类号： B60G7/02
摘要： A supporting structure for a suspension member on a vehicle includes a front cushion and a rear cushion provided between the suspension member and the body of the vehicle. The front cushion is designed with a small spring rate against a load acting longitudinally on the vehicle and a vertical load on the vehicle. The rear cushion provides a large spring rate against a vertical load on the vehicle.
摘要翻译：车辆上的悬挂构件的支撑结构包括前缓冲垫和设置在悬挂构件与车身之间的后缓冲垫。前缓冲垫设计成相对于纵向作用在车辆上的载荷具有小的弹簧刚度和车辆上的垂直载荷。后缓冲垫提供相对于车辆垂直载荷的较大的弹簧刚度。

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