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    • 11. 发明申请
    • METHOD AND KIT FOR DETECTION OF GUAIACOL-PRODUCING BACTERIUM
    • 用于检测生产GUAIACOL生产细菌的方法和试剂盒
    • US20120135456A1
    • 2012-05-31
    • US13378274
    • 2010-06-15
    • Hiroaki MurakamiMasanori Takase
    • Hiroaki MurakamiMasanori Takase
    • C12Q1/04C07C65/03
    • C12Q1/04C12Q1/045
    • Disclosed is a method for detecting guaiacol-producing bacteria in a specimen comprising culturing the specimen or a dilution thereof on a plate of a solid medium for acidophilic bacteria comprising a compound represented by the following formula, wherein R is —H, —OH, —C(O)H, —C(O)CH3, —COOH, C1-C3 alkyl or C1-C3 alkenyl, wherein alkyl and alkenyl may optionally be substituted by —OH, —C(O)H or —COOH); and detecting a colony formed on the solid medium. According to preferred embodiments, the solid medium for acidophilic bacteria comprises 50 ppm or more of vanillic acid and the plate culture is carried out at 20° C. to 55° C. The present invention also provides a solid medium for acidophilic bacteria and a kit for detection of guaiacol-producing bacteria in a specimen. According to the present invention, guaiacol-producing bacteria present in a specimen such as fruit juice raw materials can be detected rapidly in a simple manner.
    • 本发明公开了一种检测试样中愈创木酚生产菌的方法,包括将试样或其稀释液培养在含有下式表示的化合物的嗜酸细菌固体培养基板上,其中R为-H,-OH, - C(O)H,-C(O)CH 3,-COOH,C 1 -C 3烷基或C 1 -C 3烯基,其中烷基和烯基可任选被-OH,-C(O)H或-COOH取代); 并检测在固体培养基上形成的菌落。 根据优选实施方案,用于嗜酸细菌的固体培养基包含50ppm或更多的香草酸,并且板温培养在20℃至55℃下进行。本发明还提供了用于嗜酸细菌和试剂盒的固体培养基 用于在样品中检测愈创木酚生成细菌。 根据本发明,能够以简单的方式快速检测存在于果汁原料等样品中的愈创木酚生产菌。
    • 13. 发明授权
    • Method and kit for detection of guaiacol-producing bacterium
    • 用于检测愈创木酚生产菌的方法和试剂盒
    • US08921059B2
    • 2014-12-30
    • US13378274
    • 2010-06-15
    • Hiroaki MurakamiMasanori Takase
    • Hiroaki MurakamiMasanori Takase
    • G01N33/554C12Q1/04
    • C12Q1/04C12Q1/045
    • Disclosed is a method for detecting guaiacol-producing bacteria in a specimen comprising culturing the specimen or a dilution thereof on a plate of a solid medium for acidophilic bacteria comprising a compound represented by the following formula, wherein R is —H, —OH, —C(O)H, —C(O)CH3, —COOH, C1-C3 alkyl or C1-C3 alkenyl, wherein alkyl and alkenyl may optionally be substituted by —OH, —C(O)H or —COOH); and detecting a colony formed on the solid medium. According to preferred embodiments, the solid medium for acidophilic bacteria comprises 50 ppm or more of vanillic acid and the plate culture is carried out at 20° C. to 55° C. The present invention also provides a solid medium for acidophilic bacteria and a kit for detection of guaiacol-producing bacteria in a specimen. According to the present invention, guaiacol-producing bacteria present in a specimen such as fruit juice raw materials can be detected rapidly in a simple manner.
    • 本发明公开了一种检测试样中愈创木酚生产菌的方法,包括将试样或其稀释液培养在含有下式表示的化合物的嗜酸细菌固体培养基板上,其中R为-H,-OH, - C(O)H,-C(O)CH 3,-COOH,C 1 -C 3烷基或C 1 -C 3烯基,其中烷基和烯基可任选被-OH,-C(O)H或-COOH取代); 并检测在固体培养基上形成的菌落。 根据优选实施方案,用于嗜酸细菌的固体培养基包含50ppm或更多的香草酸,并且板温培养在20℃至55℃下进行。本发明还提供了用于嗜酸细菌和试剂盒的固体培养基 用于在样品中检测愈创木酚生成细菌。 根据本发明,能够以简单的方式快速检测存在于果汁原料等样品中的愈创木酚生产菌。