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11. 发明专利

DE3246090A1 未知
公开(公告)号：DE3246090A1
公开(公告)日：1984-02-16
申请号：DE3246090
申请日：1982-12-13
申请人： TOYO JOZO KK
发明人： IMAMURA SHIGEYUKI , MISAKI HIDEO
IPC分类号： G01N33/92 , C12Q1/28 , C12Q1/34 , C12Q1/44 , C12Q1/00 , C12Q1/42

12. 发明专利

DE3223874A1 未知
公开(公告)号：DE3223874A1
公开(公告)日：1983-01-20
申请号：DE3223874
申请日：1982-06-25
申请人： TOYO JOZO KK
发明人： IMAMURA SHIGEYUKI , MISAKI HIDEO , ISHIKAWA HIDEHIKO , MATSUURA KAZUO
IPC分类号： C12N9/02 , C12N9/10 , C12N9/88 , C12Q1/25 , C12N9/04 , C12N1/20 , C12Q1/60 , C12Q1/00 , C12Q1/32 , C12Q1/48 , G01N33/92
摘要： An enzyme having enoyl-CoA hydratase activity, 3-hydroxyacyl-CoA dehydrogenase activity and 3-ketoacyl-CoA thiolase activity, all in the same enzyme, is produced by culturing the microorganism strain Pseudomonas fragi B-0771 FERM-P No. 5701, and isolating the enzyme thus produced from the culture medium. Such an enzyme is useful in an assay method for a fatty acid component in a sample, which fatty acid is originally present in the sample or is liberated from a fatty acid ester in the sample, comprising: (a) converting the fatty acid to acyl-CoA; (b) converting the thus-produced acyl-CoA to dehydroacyl-CoA; (c) converting the thus-produced dehydroacyl-CoA to hydroxyacyl-CoA; (d) converting the thus-produced hydroxyacyl-CoA to ketoacyl-CoA; (e) converting the thus-produced ketoacyl-CoA to acyl-CoA; and measuring the detectable changes in the reaction mixture. A composition suitable for such lipid assay comprises ATP or GTP, CoASH, NAD, acyl-CoA synthetase activity, acyl-CoA oxidase activity, enoyl-CoA hydratase activity, 3-hydroxyacyl-CoA dehydrogenase activity, and 3-ketoacyl-CoA thiolase activity, wherein the last three activities are supplied by the new multi-active enzyme.

13. 发明专利

IT8224763D0 未知
公开(公告)号：IT8224763D0
公开(公告)日：1982-12-15
申请号：IT2476382
申请日：1982-12-15
申请人： TOYO JOZO KK
发明人： IMAMURA SHIGEYUKI , MISAKI HIDEO
IPC分类号： G01N33/92 , C12Q1/28 , C12Q1/34 , C12Q1/44 , G01N

14. 发明专利

DE2412301A1 未知
公开(公告)号：DE2412301A1
公开(公告)日：1974-09-19
申请号：DE2412301
申请日：1974-03-14
申请人： TOYO JOZO KK
发明人： HORIUCHI YOSHIFUMI , IMAMURA SHIGEYUKI
IPC分类号： C12N11/12 , C12N9/16 , C12N9/18 , C12N11/10 , C12P7/64 , C07G7/02

15. 发明专利

FR2598156B1 未知
公开(公告)号：FR2598156B1
公开(公告)日：1990-08-17
申请号：FR8706182
申请日：1987-04-30
申请人： TOYO JOZO KK
发明人： TAKAHASHI MAMURO , IMAMURA SHIGEYUKI , TAKADA MASAKI
IPC分类号： C12N9/04 , C12Q1/54
摘要： Glucose dehydrogenase having the following biochemial properties: (a) enzymatic action: catalyzes a reaction which generates glucono- delta -lactone and reduced NADP from glucose and NADP; (b) substrate specificity: has substrate specificity on glucose and no substrate specificity on 2-deoxyglucose; (c) optimum pH: pH 6-8, (d) optimum temperature: approximately 55 DEG C., (e) pH-stability: stable at pH 6.0-7.5, (f) molecular weight: 11x104+/-11000, (g) Km-value: 2.6x10-3+/-2.6x10-4M(glucose) 4.2x10-635 4.2x10-7M(NADP) and (h) isoelectric point: 4.9+/-0.5, comprises culturing a glucose dehydrogenase-producing microorganism Cryptococcus uniguttulatus Y 0033 FERM P-8709, now FERM BP-1352 in a nutrient medium and isolating glucose dehydrogenase thus produced from the cultured medium.

16. 发明专利

GB2216528A DNA sequence coding lipase 未知
公开(公告)号：GB2216528A
公开(公告)日：1989-10-11
申请号：GB8904650
申请日：1989-03-01
申请人： TOYO JOZO KK
发明人： SAGAI HITOSHI , OHTA HARUMI , SUZUKI KOJI , IMAMURA SHIGEYUKI
IPC分类号： C12N1/20 , C12N1/21 , C12N9/20 , C12N15/00 , C12N15/09 , C12R1/01 , C12R1/19

17. 发明专利

FR2536416B1 未知
公开(公告)号：FR2536416B1
公开(公告)日：1989-09-08
申请号：FR8318477
申请日：1983-11-21
申请人： TOYO JOZO KK
发明人： IMAMURA SHIGEYUKI , MISAKI HIDEO
IPC分类号： C12Q1/44 , C12Q1/34 , C12Q1/61

18. 发明专利

FR2508487B1 未知
公开(公告)号：FR2508487B1
公开(公告)日：1986-06-27
申请号：FR8211073
申请日：1982-06-24
申请人： TOYO JOZO KK
发明人： IMAMURA SHIGEYUKI , MISAKI HIDEO , ISHIKAWA HIDEHIKO , MATSUURA KAZUO
IPC分类号： C12N9/02 , C12N9/10 , C12N9/88 , C12Q1/25 , C12Q1/26
摘要： An enzyme having enoyl-CoA hydratase activity, 3-hydroxyacyl-CoA dehydrogenase activity and 3-ketoacyl-CoA thiolase activity, all in the same enzyme, is produced by culturing the microorganism strain Pseudomonas fragi B-0771 FERM-P No. 5701, and isolating the enzyme thus produced from the culture medium. Such an enzyme is useful in an assay method for a fatty acid component in a sample, which fatty acid is originally present in the sample or is liberated from a fatty acid ester in the sample, comprising: (a) converting the fatty acid to acyl-CoA; (b) converting the thus-produced acyl-CoA to dehydroacyl-CoA; (c) converting the thus-produced dehydroacyl-CoA to hydroxyacyl-CoA; (d) converting the thus-produced hydroxyacyl-CoA to ketoacyl-CoA; (e) converting the thus-produced ketoacyl-CoA to acyl-CoA; and measuring the detectable changes in the reaction mixture. A composition suitable for such lipid assay comprises ATP or GTP, CoASH, NAD, acyl-CoA synthetase activity, acyl-CoA oxidase activity, enoyl-CoA hydratase activity, 3-hydroxyacyl-CoA dehydrogenase activity, and 3-ketoacyl-CoA thiolase activity, wherein the last three activities are supplied by the new multi-active enzyme.

19. 发明专利

GB2110822A Method for the quantitative measurement of phosphatidyl glycerol 未知
公开(公告)号：GB2110822A
公开(公告)日：1983-06-22
申请号：GB8232079
申请日：1982-11-10
申请人： TOYO JOZO KK
发明人： IMAMURA SHIGEYUKI , MISAKI HIDEO
IPC分类号： G01N33/50 , C12Q1/44
摘要： A method for quantitative measurement of phosphatidyl glycerol in the liquid such as body fluid in which the fluid is treated with an enzyme to obtain glycero-3-phosphate, said enzyme being able to play as a catalyst in a reaction to produce said glycero-3- phosphate and diglyceride from phosphatidyl glycerol and water, and the liberated glycero-3-phosphate is measured. The enzyme may be phospholipase C.

20. 发明专利

FR2518117A1 未知
公开(公告)号：FR2518117A1
公开(公告)日：1983-06-17
申请号：FR8220269
申请日：1982-12-03
申请人： TOYO JOZO KK
发明人： IMAMURA SHIGEYUKI , MISAKI HIDEO
IPC分类号： G01N33/92 , C12Q1/28 , C12Q1/34 , C12Q1/44 , G01N33/50

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