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11. 发明申请

US20130316932A1 GLYCOPEPTIDE ARRAY 审中-公开
标题翻译：糖蛋白阵列
公开(公告)号：US20130316932A1
公开(公告)日：2013-11-28
申请号：US13989023
申请日：2011-11-22
申请人： Shin-Ichiro Nishimura , Takahiko Matsushita , Hideyuki Shimaoka , Wataru Takada , Kohta Igarashi , Midori Abe , Hiroki Abe , Masao Fukushima
发明人： Shin-Ichiro Nishimura , Takahiko Matsushita , Hideyuki Shimaoka , Wataru Takada , Kohta Igarashi , Midori Abe , Hiroki Abe , Masao Fukushima
IPC分类号： G01N33/68
CPC分类号： G01N33/6845 , G01N33/54353 , G01N33/54393 , G01N33/6842
摘要： The present invention provides: a glycopeptide array which is useful for the detection of the binding between a substance to be detected and a glycopeptide; and an array which can inhibit the non-specific adsorption or binding of a substance to be detected without the need of coating any adsorption-inhibiting agent and which has the glycopeptide immobilized thereon. In the present invention, it was found that a glycopeptide that has a molecule having a carbonyl group bound to a peptide moiety thereof can be immobilized on a substrate that is coated with a polymer compound containing a unit having a primary amino group with high efficiency through the carbonyl group. Particularly, it was found by the present inventors that a glycopeptide can be immobilized on a substrate with high efficiency and the non-specific adsorption or binding of a substance to be detected can be inhibited effectively when a substrate that is coated with a polymer compound containing a unit having a primary amino group, a unit for retaining hydrophilicity and a unit having a hydrophobic group is used as the substrate for the glycopeptide.
摘要翻译：本发明提供：糖肽阵列，其可用于检测待检测物质与糖肽之间的结合; 以及可以抑制待检测物质的非特异性吸附或结合而不需要涂覆任何吸附抑制剂并且其上固定有糖肽的阵列。在本发明中，发现具有与其肽部分结合的具有羰基的分子的糖肽可以固定在具有高分子量的高分子化合物的基材上，所述高分子化合物通过羰基。特别是本发明人发现，可以高效率地将糖肽固定在基材上，并且当涂布有含高分子化合物的基材时，能够有效地抑制待检测物质的非特异性吸附或结合使用具有伯氨基的单元，用于保持亲水性的单元和具有疏水基团的单元作为糖肽的底物。

12. 发明申请

US20130034557A1 Cancer-Related Glycopeptide Epitopes, Antibodies And Methods Of Use 有权
标题翻译：癌症相关糖肽表位，抗体和使用方法
公开(公告)号：US20130034557A1
公开(公告)日：2013-02-07
申请号：US13641610
申请日：2011-04-15
申请人： Shin-Ichiro Nishimura , Takahiko Matsushita , Yoshiaki Miura
发明人： Shin-Ichiro Nishimura , Takahiko Matsushita , Yoshiaki Miura
IPC分类号： C07K9/00 , A61K39/00 , A61P35/00 , A61K39/395 , G01N33/566 , A61P37/04 , C07K14/47 , C07K16/18
CPC分类号： C07K14/4727 , C07K16/3092 , C07K2317/21 , C07K2317/32 , C07K2317/34
摘要： Cancer-associated O-glycopeptide combination epitopes derived from the VNTR of MUC1 are disclosed. Autoantibodies present in human sera target the combination epitopes and are reduced or absent in cancer patients. The epitopes are useful as therapeutic and immunoprophylactic cancer vaccines. Monoclonal antibodies directed against the epitopes are also useful as immunotherapeutics for treatment and prevention of cancer. Diagnostic methods using the epitopes and antibodies are also disclosed.
摘要翻译：公开了衍生自MUC1的VNTR的癌相关O-糖肽组合表位。存在于人血清中的自身抗体靶向组合表位，并且在癌症患者中减少或不存在。表位可用作治疗和免疫预防性癌症疫苗。针对表位的单克隆抗体也可用作治疗和预防癌症的免疫治疗剂。还公开了使用表位和抗体的诊断方法。

13. 发明授权

US08148112B2 Sugar chain containing 4-position halogenated galactose and application thereof 失效
标题翻译：含有4-位卤代半乳糖的糖链及其应用
公开(公告)号：US08148112B2
公开(公告)日：2012-04-03
申请号：US11815329
申请日：2006-02-14
申请人： Shin-Ichiro Nishimura , Noriko Nagahori , Tomoki Hamamoto , Kiyoshi Okuyama , Toshitada Noguchi
发明人： Shin-Ichiro Nishimura , Noriko Nagahori , Tomoki Hamamoto , Kiyoshi Okuyama , Toshitada Noguchi
IPC分类号： C12P19/50 , C12P19/52 , C12P19/26 , C07H15/00 , C07H5/04 , C07H5/06 , C08B37/00
CPC分类号： C12P19/32 , C07H15/08
摘要： The present invention is directed to, for example, an oligosaccharide having at an end thereof a 4-position halogenated galactose residue represented by formula (I): (wherein X represents a halogen atom, and R represents a monosaccharide, an oligosaccharide, or a carrier), a transferase inhibitor containing the oligosaccharide, and a method for inhibiting sugar chain elongation reaction in the presence of glycosyltransferase, the method including employing the inhibitor. The invention also provides a method for producing a 4-position halogenated galactose sugar nucleotide represented by formula (II): (wherein each of R1 to R3 represents a hydroxyl group, an acetyl group, a halogen atom, or a hydrogen atom; X represents a halogen atom; and M represents a hydrogen ion or a metal ion), wherein the method employs bacterium-derived galactokinase and bacterium-derived hexose-1-phosphate uridylyltransferase. The invention is also directed to a sugar chain containing 4-position halogenated galactose envisaged to be employed as drugs and other materials, and to applications of the compound.
摘要翻译：本发明涉及例如末端具有由式（I）表示的4位卤代半乳糖残基的寡糖：（其中X表示卤素原子，R表示单糖，寡糖或载体），含有寡糖的转移酶抑制剂，以及在糖基转移酶存在下抑制糖链延长反应的方法，该方法包括使用该抑制剂。本发明还提供了由式（II）表示的4-位卤代半乳糖糖核苷酸的制备方法：（式中，R 1〜R 3各自表示羟基，乙酰基，卤素原子或氢原子; X表示卤素原子; M表示氢离子或金属离子），其中该方法采用细菌来源的半乳糖激酶和细菌来源的己糖-1-磷酸尿苷酰转移酶。本发明还涉及设想用作药物和其它材料的4-位卤代半乳糖的糖链以及该化合物的应用。

14. 发明授权

US06602982B1 Process for the preparation of regular glycopeptides 失效
标题翻译：制备常规糖肽的方法
公开(公告)号：US06602982B1
公开(公告)日：2003-08-05
申请号：US09744802
申请日：2001-01-30
申请人： Shin-Ichiro Nishimura
发明人： Shin-Ichiro Nishimura
IPC分类号： C07K100
CPC分类号： C07K5/0806 , A61K38/00 , C07K1/006 , C07K7/64 , C07K9/001
摘要： The present invention provides a method for easily producing glycopeptides which are useful as a material for scientific studies, medicaments and foodstuffs. The glycopeptides can be produced by coupling a fluorinated glycoside of a monosaccharide or an oligosaccharide of the formula (I) with an amino acid or a peptide and then deprotecting the resultant glycopeptide by hydrogenation, and the sequential glycopeptide of the formula (IV) can be produced by polycondensation of a precursor glycopeptide.
摘要翻译：本发明提供了一种容易地制备可用作科学研究材料，药物和食品的糖肽的方法。糖肽可以通过将式（I）的单糖或寡糖的氟化糖苷与氨基酸或肽偶联制备，然后通过氢化使所得糖肽脱保护，并且式（IV）的顺序糖肽可以是通过前体糖肽的缩聚产生。

15. 发明申请

US20130196881A1 SUGAR CHAIN ARRAY 有权
标题翻译：糖链阵列
公开(公告)号：US20130196881A1
公开(公告)日：2013-08-01
申请号：US13812787
申请日：2011-07-29
申请人： Shin-Ichiro Nishimura , Takahiko Matsushita , Hideyuki Shimaoka , Wataru Takada , Kohta Igarashi , Midori Abe , Hiroki Abe , Masao Fukushima
发明人： Shin-Ichiro Nishimura , Takahiko Matsushita , Hideyuki Shimaoka , Wataru Takada , Kohta Igarashi , Midori Abe , Hiroki Abe , Masao Fukushima
IPC分类号： G01N33/53
CPC分类号： G01N33/5308 , G01N33/54393 , G01N33/569 , G01N33/66 , G01N2333/42
摘要： A sugar chain array containing a sugar chain immobilized thereon for detecting binding between an analyte and a sugar chain, and a sugar chain array having the sugar chain immobilized thereon that is capable of inhibiting non-specific adsorption and binding of an analyte without having to coat the array with an adsorption inhibitor. A specific sugar chain is immobilized on the array, and is useful for detecting binding between the sugar chain and an analyte, such as a pathogen of an infectious disease or an excretion thereof. In addition, in the array, when a base material is coated with a polymeric compound having a unit having a primary amino group, a unit for maintaining hydrophilicity, and a unit having a hydrophobic group, the sugar chain is immobilized efficiently and non-specific adsorption and binding of the analyte can be effectively inhibited.
摘要翻译：含有固定在其上用于检测分析物和糖链之间的结合的糖链的糖链阵列和其上固定有糖链的糖链阵列，其能够抑制分析物的非特异性吸附和结合而不必涂覆该阵列带有吸附抑制剂。特定的糖链固定在阵列上，并且可用于检测糖链和分析物之间的结合，例如感染性疾病的病原体或其排泄物。此外，在阵列中，当用具有伯氨基单元的聚合化合物，保持亲水性的单元和具有疏水基团的单元涂覆基材时，糖链被有效且非特异性地固定可以有效地抑制分析物的吸附和结合。

16. 发明申请

US20130045543A1 NOVEL MUC1 ANTIBODY 有权
标题翻译：新型MUC1抗体
公开(公告)号：US20130045543A1
公开(公告)日：2013-02-21
申请号：US13643168
申请日：2011-04-28
申请人： Shin-Ichiro Nishimura , Shoichi Naito , Naoki Ohyabu , Tatsuya Takahashi , Yoshito Numata
发明人： Shin-Ichiro Nishimura , Shoichi Naito , Naoki Ohyabu , Tatsuya Takahashi , Yoshito Numata
IPC分类号： C07K16/30 , G01N33/574 , C07H21/04
CPC分类号： G01N33/57484 , A61K2039/545 , C07K16/3092 , C07K2317/34 , C07K2317/73
摘要： An objective of the present invention is to provide a MUC1 antibody having high specificity to cancer cells. The objective has been achieved by the present inventors who found that a cancer-specific sugar chain can be specifically recognized in MUC1 and a cancer cell expressing MUC1 having such a cancer cell specific sugar chain can be recognized. The present invention provides an antibody, an antigen-binding fragment thereof or a MUC1-binding molecule, having, for example, 40-fold specificity or more for a cancer-associated structure of MUC1 as compared to that of a normal tissue-associated structure of MUC1.
摘要翻译：本发明的目的是提供对癌细胞具有高特异性的MUC1抗体。本发明人已经发现，发现可以在MUC1中特异性地识别癌特异性糖链，并且可以认识到具有这样的癌细胞特异性糖链的表达MUC1的癌细胞。与正常组织相关结构相比，本发明提供了抗体，其抗原结合片段或MUC1结合分子，其与MUC1的癌症相关结构具有例如40倍特异性或更多的MUC1。

17. 发明申请

US20120309022A1 METHOD OF DETERMINATION OF RENAL CELL CARCINOMA 审中-公开
标题翻译：确定肾细胞癌的方法
公开(公告)号：US20120309022A1
公开(公告)日：2012-12-06
申请号：US13497275
申请日：2010-09-14
申请人： Shin-Ichiro Nishimura , Yoshiaki Miura , Taku Nakahara , Maho Amano , Chikara Ohyama , Shingo Hatakeyama
发明人： Shin-Ichiro Nishimura , Yoshiaki Miura , Taku Nakahara , Maho Amano , Chikara Ohyama , Shingo Hatakeyama
IPC分类号： G01N27/62
CPC分类号： G01N33/57438
摘要： The present invention provides a novel method for diagnosing renal cell carcinoma. The method of the invention allows evaluation of the presence of renal cell carcinoma or the degree of malignancy of renal cell carcinoma, by conducting comprehensive analysis of N-linked sugar chains in serum of a patient and utilizing an expression level of detected sugar chain as an index of the evaluation.
摘要翻译：本发明提供了一种诊断肾细胞癌的新方法。本发明的方法通过对患者血清中N-连接的糖链进行综合分析，并利用检测到的糖链的表达水平，作为评价肾细胞癌的存在或肾细胞癌的恶性程度评价指标。

18. 发明申请

US20090311793A1 Method of Esterifying Bio-Related Molecule for Mass Spectrometry and Method of Mass Spectrometry of Obtained Esterified Derivative 有权
标题翻译：用于质谱分析生物相关分子的方法和获得的酯化衍生物的质谱法
公开(公告)号：US20090311793A1
公开(公告)日：2009-12-17
申请号：US12224310
申请日：2007-02-22
申请人： Shin-Ichiro Nishimura , Yoshiaki Miura
发明人： Shin-Ichiro Nishimura , Yoshiaki Miura
IPC分类号： G01N33/68 , G01N24/00 , G01N33/00
CPC分类号： G01N33/6848 , C07K1/13 , Y10T436/143333 , Y10T436/24
摘要： [Problems]To provide a method for enhancing analysis sensitivity of bio-related molecules in mass spectrometry. To provide a method for rapidly and conveniently analyzing biological acid molecules using the method of enhanced analysis sensitivity in mass spectrometry. [Means for Solution] A method of preparing a bio-related molecule to be subjected to mass spectrometry, in which at least a part of acid group(s) is esterified, comprising reacting a bio-related molecule comprising an acid group(s) with a triazene compound to esterify at least a part of said acid group(s). A method of analyzing bio-related molecules comprising reacting a bio-related molecule comprising an acid group(s) with a triazene compound to esterify said acid group(s), and then subjecting said bio-related molecule having an esterified acid group(s) to mass spectrometry.
摘要翻译： [问题]提供在质谱中提高生物相关分子的分析灵敏度的方法。提供一种利用质谱法提高分析灵敏度的方法快速方便地分析生物酸分子的方法。 [解决方案]一种制备待进行质谱分析的生物相关分子的方法，其中至少一部分酸基被酯化，包括使包含酸基的生物相关分子，与三氮烯化合物酯化至少一部分所述酸基团。一种分析生物相关分子的方法，包括使包含酸基团的生物相关分子与三氮烯化合物反应以使所述酸基团酯化，然后使具有酯化酸基团的生物相关分子）进行质谱分析。

19. 发明申请

US20090187011A1 Method of serum pre-treatment for glycomic analysis 失效
标题翻译：糖尿病分析血清预处理方法
公开(公告)号：US20090187011A1
公开(公告)日：2009-07-23
申请号：US12308936
申请日：2007-06-29
申请人： Shin-Ichiro Nishimura , Yasuro Shinohara , Yoshiaki Miura , Jun-ichi Furukawa , Yoko Kita , Akio Takimoto , Mika Nakano
发明人： Shin-Ichiro Nishimura , Yasuro Shinohara , Yoshiaki Miura , Jun-ichi Furukawa , Yoko Kita , Akio Takimoto , Mika Nakano
IPC分类号： C07K1/12 , C07C233/02
CPC分类号： C07C309/15 , C12P19/00 , G01N33/66
摘要： A means for pre-treatment in glycomic analysis of a glycoprotein is provided by the present invention.A salt of the general formula (I): wherein Z, X, R1, R2, M, m and n are the same as described in DESCRIPTION, is useful as a protein solubilizer, and an oligosaccharide is efficiently released from a sample if reductive alkylation and/or digestion by a proteinase are carried out under the presence of the said solubilizer at the first step in the glycomic analysis of glycoprotein derived from a living body.
摘要翻译：本发明提供了糖蛋白糖基分析中预处理的手段。通式（I）的盐：其中Z，X，R 1，R 2，M，m和n与描述中所述相同，可用作蛋白质增溶剂，并且如果还原，寡糖有效地从样品中释放通过蛋白酶的烷基化和/或消化在所述增溶剂的存在下在来自活体的糖蛋白的糖基分析中的第一步进行。

20. 发明申请

US20090018327A1 SUGAR CHAIN CONTAINING 4-POSITION HALOGENATED GALACTOSE AND APPLICATION THEREOF 失效
标题翻译：含有4-位羟基糖的糖链及其应用
公开(公告)号：US20090018327A1
公开(公告)日：2009-01-15
申请号：US11815329
申请日：2006-02-14
申请人： Shin-Ichiro Nishimura , Noriko Nagahori , Tomoki Hamamoto , Kiyoshi Okuyama , Toshitada Noguchi
发明人： Shin-Ichiro Nishimura , Noriko Nagahori , Tomoki Hamamoto , Kiyoshi Okuyama , Toshitada Noguchi
IPC分类号： C07H5/02 , C12P19/30
CPC分类号： C12P19/32 , C07H15/08
摘要： The present invention is directed to, for example, an oligosaccharide having at an end thereof a 4-position halogenated galactose residue represented by formula (I): (wherein X represents a halogen atom, and R represents a monosaccharide, an oligosaccharide, or a carrier), a transferase inhibitor containing the oligosaccharide, and a method for inhibiting sugar chain elongation reaction in the presence of glycosyltransferase, the method including employing the inhibitor.The invention also provides a method for producing a 4-position halogenated galactose sugar nucleotide represented by formula (II): (wherein each of R1 to R3 represents a hydroxyl group, an acetyl group, a halogen atom, or a hydrogen atom; X represents a halogen atom; and M represents a hydrogen ion or a metal ion), wherein the method employs bacterium-derived galactokinase and bacterium-derived hexose-1-phosphate uridylyltransferase.The invention is also directed to a sugar chain containing 4-position halogenated galactose envisaged to be employed as drugs and other materials, and to applications of the compound.
摘要翻译：本发明涉及例如末端具有由式（I）表示的4-位卤代半乳糖残基的寡糖：（其中，X表示卤素原子，R表示单糖，寡糖，或载体），含有寡糖的转移酶抑制剂，以及在糖基转移酶存在下抑制糖链延长反应的方法，该方法包括使用该抑制剂。本发明还提供了由式（II）表示的4-位卤代半乳糖糖核苷酸的制备方法：（式中，R 1〜R 3各自表示羟基，乙酰基，卤素原子或氢原子; X表示卤素原子; M表示氢离子或金属离子），其中该方法采用细菌来源的半乳糖激酶和细菌来源的己糖-1-磷酸尿苷酰转移酶。本发明还涉及设想用作药物和其它材料的4-位卤代半乳糖的糖链以及该化合物的应用。

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