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11. 外观设计

USD747991S1 Touch pad for destination selection controller 有权
标题翻译：触摸板用于目的地选择控制器
公开(公告)号：USD747991S1
公开(公告)日：2016-01-26
申请号：US29487386
申请日：2014-04-08
申请人： Danny Lee , Han U. Lee
设计人： Danny Lee , Han U. Lee

12. 外观设计

USD747990S1 Key pad for destination selection controller 有权
标题翻译：目的地选择控制器的键盘
公开(公告)号：USD747990S1
公开(公告)日：2016-01-26
申请号：US29487366
申请日：2014-04-08
申请人： Danny Lee , Han U. Lee
设计人： Danny Lee , Han U. Lee

13. 发明申请

US20120108440A1 REDUCING ADAPTER DIMER FORMATION 有权
标题翻译：减少适应器二次形成
公开(公告)号：US20120108440A1
公开(公告)日：2012-05-03
申请号：US12952563
申请日：2010-11-23
申请人： Patrick Lau , Danny Lee
发明人： Patrick Lau , Danny Lee
IPC分类号： C40B20/00 , C40B50/12 , C40B50/06 , C07H21/00
CPC分类号： C12N15/1068 , C12Q1/6855 , C40B50/06 , C12Q2537/163 , C12Q2533/107 , C12Q2525/301 , C12Q2525/197
摘要： Provided herein is a method of reducing adapter dimer formation comprising contacting a sample comprising target nucleic acid sequences with 5′ and 3′ adapters in the presence of one or more hairpin oligonucleotides. Also provided is a method of preparing a library of nucleic acid sequences comprising contacting first adapter oligonucleotides with a sample comprising target nucleic acid sequences under conditions to form first ligation products, contacting the sample with one or more hairpin oligonucleotides that binds to the first adapter oligonucleotides, and contacting the sample with second adapter oligonucleotides under conditions to bind to the first ligation products and form second ligation products, wherein the second ligation products form the library of nucleic acid sequences.
摘要翻译：本文提供了减少适配体二聚体形成的方法，包括在一种或多种发夹寡核苷酸存在下使包含靶核酸序列的样品与5'和3'衔接子接触。还提供了制备核酸序列文库的方法，包括在条件下使第一衔接子寡核苷酸与包含靶核酸序列的样品接触以形成第一连接产物，使样品与一种或多种结合第一衔接子寡核苷酸的发夹寡核苷酸接触并且在与第一连接产物结合的条件下使样品与第二衔接子寡核苷酸接触并形成第二连接产物，其中第二连接产物形成核酸序列文库。

14. 发明申请

US20070212704A1 COMPOSITIONS, METHODS, AND KITS FOR AMPLIFYING NUCLEIC ACIDS 审中-公开
标题翻译：用于放大核酸的组合物，方法和试剂盒
公开(公告)号：US20070212704A1
公开(公告)日：2007-09-13
申请号：US11537409
申请日：2006-09-29
申请人： Shoulian Dong , Junko Stevens , Danny Lee
发明人： Shoulian Dong , Junko Stevens , Danny Lee
IPC分类号： C12Q1/68 , C12P19/34 , C12N9/99 , C07H21/02
CPC分类号： C12Q1/6846 , C12N15/115 , C12N2310/16 , C12P19/34 , C12Q1/6848 , G01N21/6486 , C12Q2549/125 , C12Q2527/127 , C12Q2527/107
摘要： The present teachings are directed to compositions, methods, and kits for amplifying target nucleic acids while reducing non-specific fluorescence and undesired amplification products, sometimes referred to as secondary amplification products or spurious side-products. The enzyme inhibitors disclosed herein comprise a nucleotide sequence and at least one quencher. Complexes comprising an enzyme inhibitor associated with an enzyme, wherein at least one enzymatic activity of the enzyme is inhibited, are also provided. Methods for amplifying a target nucleic acid while reducing undesired amplification products are disclosed, as are methods for reducing non-specific fluorescence. Kits for expediting the performance of certain disclosed methods are also provided.
摘要翻译：本教导涉及用于扩增靶核酸的组合物，方法和试剂盒，同时减少非特异性荧光和不需要的扩增产物，有时称为二级扩增产物或假副产物。本文公开的酶抑制剂包含核苷酸序列和至少一种猝灭剂。还提供了包含与酶相关的酶抑制剂的复合物，其中所述酶的至少一种酶活性被抑制。公开了减少不期望的扩增产物同时扩增靶核酸的方法，还有用于降低非特异性荧光的方法。还提供了用于加速某些公开方法的执行的套件。

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