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11. 发明授权

US5633143A Method for the quantitative determination of D-3-hydroxybutyric acid and acetoacetic acid, and analytical reagent therefor 失效
标题翻译：定量测定D-3-羟基丁酸和乙酰乙酸的方法及其分析试剂
公开(公告)号：US5633143A
公开(公告)日：1997-05-27
申请号：US244450
申请日：1994-05-26
申请人： Shigeru Ueda , Hideo Misaki , Shigeru Ikuta , Mamoru Takahashi
发明人： Shigeru Ueda , Hideo Misaki , Shigeru Ikuta , Mamoru Takahashi
IPC分类号： C12Q1/32 , C12Q1/52 , G01N31/00 , G01N33/53
CPC分类号： C12Q1/32 , Y10S435/966 , Y10S435/973 , Y10T436/104998
摘要： Disclosed is a method for the quantitative determination of D-3-hydroxybutyric acid and acetoacetic acid, which comprises reacting a biological sample containing D-3-hydroxybutyric acid and acetoacetic acid, with a reagent comprising: (1) a D-3-hydroxybutyrate dehydrogenase, (2) A.sub.1 and (3) B.sub.1, the components (1), (2) and (3) participating in the following cycling reaction: ##STR1## thereby effecting the enzymatic cycling reaction, and measuring a change in the amount of A.sub.2 formed or the amount of B.sub.1 consumed. Also disclosed is an analytical reagent comprising the components (1), (2) and (3) for use in the above method. The method and the analytical reagent ensure rapidness and accuracy in the determination of D-3-hydroxybutyric acid and acetoacetic acid, even with the use of a small quantity of a biological sample, so that they are very useful in application fields, such as clinical diagnosis and food testing.
摘要翻译： PCT No.PCT / JP91 / 01706 Sec。 371日期：1994年5月26日 102（e）日期1994年5月26日PCT 1991年12月12日PCT PCT。公开号WO93 / 12254 日期：1993年6月24日公开是定量测定D-3-羟基丁酸和乙酰乙酸的方法，其包括使含有D-3-羟基丁酸和乙酰乙酸的生物样品与包含以下物质的试剂反应：（1） D-3-羟基丁酸脱氢酶，（2）A1和（3）B1，参与以下循环反应的组分（1），（2）和（3）：进行酶循环反应，并测量形成的A2的量的变化或消耗的B1的量。还公开了包含用于上述方法的组分（1），（2）和（3）的分析试剂。该方法和分析试剂确保即使使用少量生物样品也能快速，准确地测定D-3-羟基丁酸和乙酰乙酸，因此它们在临床应用领域非常有用诊断和食品检测。

12. 发明授权

US5780256A Method and composition for quantitative determination of ammonia, .alpha.-amino acid, or .alpha.-keto acid 失效
标题翻译：用于定量测定氨，α-氨基酸或α-酮酸的方法和组合物
公开(公告)号：US5780256A
公开(公告)日：1998-07-14
申请号：US108736
申请日：1995-03-13
申请人： Shigeru Ueda , Mamoru Takahashi , Hideo Misaki , Shigeru Ikuta
发明人： Shigeru Ueda , Mamoru Takahashi , Hideo Misaki , Shigeru Ikuta
IPC分类号： C12Q1/32
CPC分类号： C12Q1/32
摘要： The present invention is directed to a method for the quantitative determination of ammonia, an .alpha.-amino acid and an .alpha.-keto acid corresponding to the .alpha.-amino acid, or a chemical substance producing any one of these compounds. The present invention is also directed to an analytical composition for use in the above method. The method of the present invention ensures rapidness and accuracy in the determination of ammonia, .alpha.-amino acids or .alpha.-keto acids, even with the use of a small quantity of a biological sample. This method is very useful in application fields, such as clinical diagnosis and food testing.
摘要翻译： PCT No.PCT / JP91 / 01785 Sec。 371日期1995年3月13日 102（e）1995年3月13日PCT PCT 1991年12月27日PCT公布。 WO92 / 15705 PCT公开号日期1992年9月17日本发明涉及一种定量测定对应于α-氨基酸的氨，α-氨基酸和α-酮酸的方法，或者是产生这些化合物中的任何一种的化学物质。本发明还涉及用于上述方法的分析组合物。本发明的方法即使使用少量的生物样品也能确保氨，α-氨基酸或α-酮酸的测定的快速和准确性。该方法在临床诊断和食品检测等应用领域非常有用。

13. 发明授权

US4347323A Glycerol kinase from Streptomyces canus 失效
标题翻译：来自链霉菌属的甘油激酶
公开(公告)号：US4347323A
公开(公告)日：1982-08-31
申请号：US154029
申请日：1980-05-28
申请人： Shigeyuki Imamura , Tohru Matsumoto , Naoki Muto , Hideo Misaki
发明人： Shigeyuki Imamura , Tohru Matsumoto , Naoki Muto , Hideo Misaki
IPC分类号： C12N9/12 , C12R1/465 , C12Q1/48
CPC分类号： C12N9/12 , Y10S435/886
摘要： A novel glycerol kinase is produced from the microorganism Streptomyces canus FERM-P No. 4977 and is useful as a diagnostic reagent for assay of triglycerides and glycerol in body fluids such as serum.
摘要翻译：从微生物链霉菌FERM-P No.4977生产新型甘油激酶，可用作体液如血清中甘油三酯和甘油的诊断试剂。

14. 发明授权

US4525458A Method for the production of sphingomyelinase 失效
标题翻译：生产鞘磷脂酶的方法
公开(公告)号：US4525458A
公开(公告)日：1985-06-25
申请号：US413677
申请日：1982-09-01
申请人： Shigeyuki Imamura , Hideo Misaki , Naoki Muto
发明人： Shigeyuki Imamura , Hideo Misaki , Naoki Muto
IPC分类号： C12N9/16 , C12R1/465
CPC分类号： C12N9/16 , Y10S435/886
摘要： The invention is to provide a method for the production of sphingomyelinase, by culturing in a medium a sphingomyelinase-producing strain belonging to genus Streptomyces and collecting the resulting sphingomyelinase from the cultured medium. The strain, Streptomyces sp. A 9107, newly separated from a soil, is found preferable for the method of the present invention, and has been deposited as NRRL 15100 and FERM-P 4978.
摘要翻译：本发明提供一种通过在培养基中培养属于链霉菌属的产生鞘磷脂酶的菌株并从培养基中收集得到的鞘磷脂酶来提供鞘磷脂酶的生产方法。菌株，链霉菌属对于本发明的方法，优选新的与土壤分离的9107，并且已经保藏为NRRL 15100和FERM-P 4978。

15. 发明授权

US5089393A Assay method for phosphatidyl ethanolamine 失效
标题翻译：磷酸二乙酯胺的测定方法
公开(公告)号：US5089393A
公开(公告)日：1992-02-18
申请号：US176235
申请日：1988-03-31
申请人： Shigeyuki Imamura , Hideo Misaki
发明人： Shigeyuki Imamura , Hideo Misaki
IPC分类号： G01N33/92 , C12Q1/26 , C12Q1/44
CPC分类号： C12Q1/44 , C12Q1/26 , C12Q2326/96 , G01N2333/906 , G01N2333/916 , G01N2405/04 , Y10S435/832
摘要： Ethanolamine in a sample can be assayed by treating the sample with ethanolamine oxidase, thereby to catalyze a reaction-consuming ethanolamine, oxygen and water, and forming glycolaldehyde, ammonia and hydrogen peroxide. The amount of consumed oxygen or the amount of generated ammonia or hydrogen peroxide is then determined, as a measure of the ethanolamine that was originally the sample. The ethanolamine can appear in the sample as such, or can be liberated simultaneously with or prior to the catalysis reaction, from an ethanolamine derivative, e.g. phosphatidyl ethanolamine by the action of phospholipase D. Ethanolamine oxidase can be produced from Bacillus sp. B-0783 FERM-P No. 5798 in a conventional culture medium, preferably by submerged aeration liquid culturation.

16. 发明授权

US4845028A Composition for lipase assay 失效
标题翻译：脂肪酶测定组合物
公开(公告)号：US4845028A
公开(公告)日：1989-07-04
申请号：US244513
申请日：1988-09-09
申请人： Shigeyuki Imamura , Hideo Misaki
发明人： Shigeyuki Imamura , Hideo Misaki
IPC分类号： C12Q1/44 , C12Q1/34 , C12Q1/61
CPC分类号： C12Q1/34 , G01N2333/918
摘要： A composition and method for lipase assay, comprising the use of an aqueous solution of a 1,2-diglyceride of a higher fatty acid, and a nonionic surface active agent. The higher fatty acid is a higher fatty acid of 8 or more and preferably 12 or more carbons, most preferably a higher unsaturated fatty acid of more than 16 carbons. The concentration of 1,2-diglyceride is more than 0.5 g per liter of solution. The nonionic surface active agent is a polyoxyethylene-type nonionic surface active agent, a polyhydric-alcohol-type nonionic surface active agent or a block-polymer-type nonionic surface active agent, whose concentration is more than 0.1% by weight in the composition and which has an HLB more than 10. The composition preferably contains 0.5-10 g of 1,2-diglyceride and 10-50 g of nonionic surface active agent per liter of solution.
摘要翻译：脂肪酶测定的组合物和方法，包括使用高级脂肪酸的1,2-二甘油酯的水溶液和非离子表面活性剂。高级脂肪酸是8个以上且优选12个或更多个碳的高级脂肪酸，最优选高于16个碳的较高不饱和脂肪酸。 1,2-二甘油酯的浓度高于每升溶液0.5g。非离子表面活性剂是组合物中浓度大于0.1重量％的聚氧乙烯型非离子表面活性剂，多元醇型非离子表面活性剂或嵌段聚合物型非离子表面活性剂，其HLB大于10.该组合物优选每升溶液含有0.5-10g的1,2-二甘油酯和10-50g的非离子表面活性剂。

17. 发明授权

US4788147A Method for producing ethanolamine oxidase 失效
标题翻译：生产乙醇胺氧化酶的方法
公开(公告)号：US4788147A
公开(公告)日：1988-11-29
申请号：US865584
申请日：1986-05-21
申请人： Shigeyuki Imamura , Hideo Misaki
发明人： Shigeyuki Imamura , Hideo Misaki
IPC分类号： G01N33/92 , C12Q1/26 , C12Q1/44 , C12N9/06
CPC分类号： C12Q1/44 , C12Q1/26 , C12Q2326/96 , G01N2333/906 , G01N2333/916 , G01N2405/04 , Y10S435/832
摘要： Ethanolamine in a sample can be assayed by treating the sample with ethanolamine oxidase, thereby to catalyze a reaction-consuming ethanolamine, oxygen and water, and forming glycolaldehyde, ammonia and hydrogen peroxide. The amount of consumed oxygen or the amount of generated ammonia or hydrogen peroxide is then determined, as a measure of the ethanolamine that was originally the sample. The ethanolamine can appear in the sample as such, or can be liberated simultaneously with or prior to the catalysis reaction, from an ethanolamine derivative, e.g. phosphatidyl ethanolamine by the action of phospholipase D. Ethanolamine oxidase can be produced from Bacillus sp. B-0783 FERM-P No. 5798 in a conventional culture medium, preferably by submerged aeration liquid culturation.
摘要翻译：通过用乙醇胺氧化酶处理样品可以测定样品中的乙醇胺，从而催化消耗反应的乙醇胺，氧和水，并形成乙二醇，氨和过氧化氢。然后测定消耗的氧气的量或产生的氨或过氧化氢的量，作为最初为样品的乙醇胺的量度。乙醇胺可以如此出现在样品中，或者可以与催化反应同时或之前从乙醇胺衍生物例如乙醇胺衍生物中释放出来。磷脂酰乙醇胺通过磷脂酶D的作用。乙醇胺氧化酶可以从芽孢杆菌sp。 B-0783 FERM-P No.5798，优选通过浸没曝气液体培养。

18. 发明授权

US4496655A Process for the production of tyramine oxidase 失效
标题翻译：酪胺氧化酶生产工艺
公开(公告)号：US4496655A
公开(公告)日：1985-01-29
申请号：US454060
申请日：1982-12-28
申请人： Eiichi Yoshino , Kazuo Matsuura , Hideo Misaki
发明人： Eiichi Yoshino , Kazuo Matsuura , Hideo Misaki
IPC分类号： C12N9/06 , C12R1/06 , C12Q1/26 , C12Q1/36
CPC分类号： C12N9/0022 , Y10S435/83
摘要： Tyramine oxidase, which is useful for the clinical diagnosis of leucine aminopeptidase activity in serum, is produced by culturing the microorganism Arthrobacter sp. B-0813 FERM-P No. 6240 in a nutrient medium, and isolating thus-produced tyramine oxidase from the cultured medium.
摘要翻译：酪氨酸氧化酶可用于血清中亮氨酸氨基肽酶活性的临床诊断，是通过培养微生物节杆菌属（Arthrobacter sp。 B-0813 FERM-P No.6240在营养培养基中，并从培养基中分离由此产生的酪胺氧化酶。

19. 发明授权

US4420562A Method for producing creatinase 失效
标题翻译：生成肌酸酐酶的方法
公开(公告)号：US4420562A
公开(公告)日：1983-12-13
申请号：US371458
申请日：1982-04-23
申请人： Shigeru Ikuta , Kazuo Matsuura , Hideo Misaki
发明人： Shigeru Ikuta , Kazuo Matsuura , Hideo Misaki
IPC分类号： C12N9/78 , C12N9/80 , C12R1/07
CPC分类号： C12R1/07 , C12N9/78 , Y10S435/815 , Y10S435/832
摘要： Isolation method of creatinase is disclosed. The method comprises culturing a microorganism belonging to Bacillus, for example, B-0618 strain (deposition No. FERM-P 4049 at Fermentation Research Institute, Agency of Industrial Science and Technology, Japan) to obtain cells from the cultured product, obtaining sarcosine oxidase and creatinase-containing solution, fractionally eluting sarcosine oxidase and creatinase by anion exchange chromatography to obtain a creatinase fraction and then collecting creatinase.
摘要翻译：公开了肌酸酐酶的分离方法。该方法包括培养属于芽孢杆菌的微生物，例如B-0618菌株（日本工业科学技术学院发酵研究所的淀粉编号FERM-P4049），从培养物中获得细胞，得到肌氨酸氧化酶和含肌酸酐溶液，通过阴离子交换色谱法分级洗脱肌氨酸氧化酶和肌酸酐酶，得到肌酸酐酶级分，然后收集肌酸酐酶。

20. 发明授权

US4683198A Novel maltose dehydrogenase, process for its production, and analytical method using the same 失效
标题翻译：新型麦芽糖脱氢酶，其生产方法和使用其的分析方法
公开(公告)号：US4683198A
公开(公告)日：1987-07-28
申请号：US674009
申请日：1984-11-23
申请人： Hidehiko Ishikawa , Kazuo Matsuura , Hideo Misaki
发明人： Hidehiko Ishikawa , Kazuo Matsuura , Hideo Misaki
IPC分类号： C12N9/04 , C12Q1/32 , C12R1/44 , C12Q1/40
CPC分类号： C12N9/0006 , C12Q1/32 , Y10S435/882 , Y10S435/883 , Y10S435/884
摘要： An enzyme which acts on a reducing terminal of a monosaccharide or oligosaccharide without requiring NAD or NADP and which catalyzes the reaction ##STR1## wherein R is a saccharide chain residue or hydrogen, A is a hydrogen acceptor other than NAD or NADP, AH or AHn is a reduced form acceptor and n is 1 or 2. This maltose dehydrogenase is produced by culturing a microorganism belonging to genus Staphylococcus, specifically, sp. B-0875 FERM BP-385, and isolating the thus-produced maltose dehydrogenase from the culture medium. An assay method for the determination of saccharide or the activity of a saccharide liberating enzyme, comprises reacting this enzyme with a substrate in the presence of a hydrogen acceptor, and measuring the amount of a detectable change.
摘要翻译：作用于单糖或寡糖的还原末端而不需要NAD或NADP并且催化反应的酶，其中R是糖链残基或氢，A是NAD以外的氢受体或 NADP，AH或AHn是还原型受体，n是1或2.这种麦芽糖脱氢酶是通过培养属于葡萄球菌属的微生物，特别是sp。 B-0875FERM BP-385，并从培养基中分离由此产生的麦芽糖脱氢酶。用于测定糖类或糖释放酶活性的测定方法包括在氢受体存在下使该酶与底物反应，并测量可检测的变化量。

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