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11. 发明申请

US20090282582A1 ENVIRONMENTAL STRESS RESPONSIVE PROMOTER AND METHOD OF TISSUE-SPECIFIC GENE EXPRESSION USING THE SAME 审中-公开
标题翻译：环境应激反应促进剂及使用其组织特异性基因表达的方法
公开(公告)号：US20090282582A1
公开(公告)日：2009-11-12
申请号：US12311900
申请日：2007-10-19
申请人： Motoaki Seki , Jong-Myong Kim , Kazuo Shinozaki , Miki Fujita
发明人： Motoaki Seki , Jong-Myong Kim , Kazuo Shinozaki , Miki Fujita
IPC分类号： C12N15/82 , C07H21/04 , C12N15/63 , A01H5/00 , C12N5/10
CPC分类号： C07K14/415 , C12N15/8237
摘要： This invention concerns induction of gene expression specifically in given tissue with the use of a novel promoter that has environmental stress responsiveness and functions specifically in given tissue. It is realized by a method comprising: a step of preparing a plant having an arbitrary gene downstream of the environmental stress responsive promoter comprising DNA (a), (b), or (c) below; (a) DNA comprising the nucleotide sequence as shown in any of SEQ ID NO: 1 to 8; (b) DNA comprising a nucleotide sequence derived from the nucleotide sequence as shown in any of SEQ ID NO: 1 to 8 by deletion, substitution, or addition of one or a plurality of nucleotides and functioning as an environmental stress responsive promoter; or (c) DNA hybridizing under stringent conditions to DNA comprising the nucleotide sequence as shown in any of SEQ ID NO: 1 to 8 and functioning as an environmental stress responsive promoter; and a step of cultivating the plant in the presence of environmental stress, the method comprising inducing expression of the gene located downstream of the environmental stress responsive promoter in a tissue-specific manner.
摘要翻译：本发明涉及使用具有环境应激反应性和特定于给定组织中功能的新型启动子来特异性诱导给定组织中的基因表达。通过以下方法实现：包括下述DNA（a），（b）或（c）的环境胁迫响应启动子下游具有任意基因的植物的步骤; （a）包含SEQ ID NO：1至8中任一个所示的核苷酸序列的DNA; （b）通过缺失，取代或添加一个或多个核苷酸并起到环境应激反应启动子的作用，其包含衍生自SEQ ID NO：1至8中任一项所示核苷酸序列的核苷酸序列的DNA; 或（c）在严格条件下与包含SEQ ID NO：1至8中任一所示的核苷酸序列的DNA杂交并用作环境胁迫响应启动子的DNA的DNA; 以及在存在环境胁迫的情况下培育植物的步骤，所述方法包括以组织特异性方式诱导位于环境胁迫响应启动子下游的基因的表达。

12. 发明授权

US07608758B2 Plant system for comprehensive gene function analysis with the use of full-length cDNA 失效
标题翻译：使用全长cDNA进行综合基因功能分析的植物系统
公开(公告)号：US07608758B2
公开(公告)日：2009-10-27
申请号：US10488087
申请日：2002-08-29
申请人： Minami Matsui , Takanari Ichikawa , Miki Nakazawa , Motoaki Seki , Miki Fujita , Kazuo Shinozaki
发明人： Minami Matsui , Takanari Ichikawa , Miki Nakazawa , Motoaki Seki , Miki Fujita , Kazuo Shinozaki
IPC分类号： C12N15/82
CPC分类号： C12N15/8216
摘要： A gene causative of a phenotype is easily specified and thus gene functions are comprehensively analyzed. A method for analyzing a gene function includes (a) infecting a plant group with a full-length cDNA library containing an expression regulatory sequence to introduce the cDNA into the plants; (b) selecting a plant complying with desired selection requirements from the plants into which the cDNA has been introduced; (c) isolating the cDNA from the selected plant; and (d) re-introducing the thus isolated cDNA into a plant of the same species as the plant into which the cDNA has been introduced, to reconfirm a phenotype according to the selection requirements.
摘要翻译：一个易于指定表型的基因，从而全面分析基因功能。用于分析基因功能的方法包括（a）用含有表达调控序列的全长cDNA文库感染植物组，以将cDNA引入植物中; （b）从已经引入cDNA的植物中选择符合所需选择要求的植物; （c）从所选择的植物中分离cDNA; 和（d）将如此分离的cDNA重新引入与引入cDNA的植物相同物种的植物中，以根据选择要求重新确认表型。

13. 发明授权

US07368630B2 Environmental stress-responsive promoter and a gene encoding environmental stress-responsive transcriptional factor 失效
公开(公告)号：US07368630B2
公开(公告)日：2008-05-06
申请号：US11470154
申请日：2006-09-05
申请人： Kazuo Shinozaki , Motoaki Seki , Miki Fujita
发明人： Kazuo Shinozaki , Motoaki Seki , Miki Fujita
IPC分类号： C12N15/82 , A01H1/00
CPC分类号： C12N15/8238 , C07K14/415 , C12N15/8237 , C12N15/8273
摘要： The present invention provides a method of regulating expression of a gene by (a) preparing a recombinant plant cell line, plant tissue or plant with an expression vector having an abiotic environmental stress-responsive promoter of SEQ ID NO: 27 and the gene downstream thereof; and (b) culturing and cultivating the recombinant plant cell, plant tissue or plant under an abiotic environmental stress, wherein the promoter regulates the expression of the gene under the abiotic environmental stress.

14. 发明授权

US4883780A Aluminum nitride sintered body and preparation thereof 失效
标题翻译：氮化铝烧结体及其制备方法
公开(公告)号：US4883780A
公开(公告)日：1989-11-28
申请号：US157150
申请日：1988-02-11
申请人： Mituo Kasori , Kazuo Shinozaki , Kazuo Anzai , Akihiko Tsuge , Hiroshi Imagawa , Takeshi Takano , Fumio Ueno , Akihiro Horiguchi , Hiroshi Inoue
发明人： Mituo Kasori , Kazuo Shinozaki , Kazuo Anzai , Akihiko Tsuge , Hiroshi Imagawa , Takeshi Takano , Fumio Ueno , Akihiro Horiguchi , Hiroshi Inoue
IPC分类号： C04B35/58 , C04B35/581
CPC分类号： C04B35/581
摘要： There are disclosed an aluminum sintered body comprising(a) aluminum nitride,(b) at least one compound selected from the group consisting of an aluminum compound of a rare earth metal, an aluminum compound of an alkaline earth metal, and an aluminum compound of a rare earth metal and an alkaline earth metal, and(c) at least one element selected from the transition elements consisting of Groups IVa, Va, VIa, VIIa and VIII of the periodic table, and/or at least one compound containing said element,and a process for preparing the same comprising mixing aluminum nitride with(i) at least one of compound selected from the group consisting of a rare earth metal and/or an alkaline earth metal; and(ii) at least one of element selected from the group consisting of a transition element of Groups IVa, Va, VIa, VIIa and VIII of the periodic table, and/or at least one of a compound containing said element;and then molding and sintering the mixture.
摘要翻译：公开了一种铝烧结体，其包含（a）氮化铝，（b）至少一种选自稀土金属的铝化合物，碱土金属的铝化合物和铝化合物的化合物稀土金属和碱土金属，和（c）选自元素周期表第Ⅳa，Ⅴa，Ⅵa，Ⅶa和Ⅷ族的过渡元素中的至少一种元素，和/或含有所述元素的至少一种化合物和其制备方法包括将氮化铝与（i）选自稀土金属和/或碱土金属的化合物中的至少一种化合物混合; 和（ii）选自元素周期表第Ⅳa，Ⅴa，Ⅵa，Ⅶa和Ⅷ族的过渡元素的元素中的至少一种，和/或含有所述元素的化合物中的至少一种; 然后成型和烧结混合物。

15. 发明授权

US4746637A Aluminum nitride sintered body and process for producing the same 失效
标题翻译：氮化铝烧结体及其制造方法
公开(公告)号：US4746637A
公开(公告)日：1988-05-24
申请号：US760772
申请日：1985-07-31
申请人： Mituo Kasori , Kazuo Shinozaki , Kazuo Anzai , Akihiko Tsuge
发明人： Mituo Kasori , Kazuo Shinozaki , Kazuo Anzai , Akihiko Tsuge
IPC分类号： C04B35/581 , C04B35/58
CPC分类号： C04B35/581
摘要： Disclosed are an aluminum nitride sintered body comprising aluminum nitride, rare earth element-aluminum compounds and alkaline earth metal-aluminum compounds, and a process for producing an aluminum nitride sintered body, which comprises adding to aluminum nitride powder:(a) powder of at least one compound selected from the group consisting of rare earth element oxides, rare earth element fluorides and compounds capable of being converted into these oxides or fluorides by calcination, and(b) powder of at least one compound selected from the group consisting of alkaline earth metal oxides, alkali earth metal fluorides and compounds capable of being converted into these oxides or fluorides by calcination, in a total amount of 0.01 to 20 wt % as calculated on the weight of the oxides or the fluorides, and then molding and sintering the resultant mixture.
摘要翻译：公开了一种包含氮化铝，稀土元素 - 铝化合物和碱土金属 - 铝化合物的氮化铝烧结体，以及一种制造氮化铝烧结体的方法，其包括向氮化铝粉末中加入：（a）选自稀土元素氧化物，稀土元素氟化物和能够通过煅烧转化为这些氧化物或氟化物的化合物中的至少一种化合物，和（b）至少一种选自碱土金属的化合物的粉末金属氧化物，碱土金属氟化物和能够通过煅烧转化为这些氧化物或氟化物的化合物，其总量按照氧化物或氟化物的重量计算为0.01〜20重量％，然后成型和烧结所得物混合物。

16. 发明申请

US20130019340A1 PLANT RESISTANT TO ENVIRONMENTAL STRESS AND METHOD FOR PRODUCING THE SAME 审中-公开
标题翻译：耐环境应力的植物及其生产方法
公开(公告)号：US20130019340A1
公开(公告)日：2013-01-17
申请号：US13635390
申请日：2011-03-01
申请人： Kaoru Maruyama , Kazuo Shinozaki
发明人： Kaoru Maruyama , Kazuo Shinozaki
IPC分类号： C12N15/82
CPC分类号： C07K14/415 , C12N15/8269 , C12N15/8271 , C12N15/8273
摘要： An object of the present invention is to provide a plant having environmental stress resistance. The present invention provides a transgenic plant having environmental stress resistance, in which an exogenous DNA encoding a transcription factor selected from the group consisting of transcription factors from Arabidopsis thaliana TCP13, TCP5 and TCP17, and homologous transcription factors thereof from other plants is overexpressed, and a method for producing such a plant.
摘要翻译：本发明的目的是提供一种具有耐环境胁迫性的植物。本发明提供了具有环境胁迫性的转基因植物，其中编码选自拟南芥TCP13，TCP5和TCP17的转录因子的转录因子的外源DNA以及来自其他植物的同源转录因子被过表达，一种生产这种植物的方法。

17. 发明申请

US20080216196A1 Environmental stress-responsive promoter and a gene encoding environmental stress-responsive transcriptional factor 审中-公开
标题翻译：环境应激反应启动子和编码环境应激反应转录因子的基因
公开(公告)号：US20080216196A1
公开(公告)日：2008-09-04
申请号：US12076932
申请日：2008-03-25
申请人： Kazuo Shinozaki , Motoaki Seki , Miki Fujita
发明人： Kazuo Shinozaki , Motoaki Seki , Miki Fujita
IPC分类号： C12N15/29 , C12N15/82
CPC分类号： C12N15/8238 , C07K14/415 , C12N15/8237 , C12N15/8273
摘要： The present invention provides a method of regulating expression of a gene by (a) preparing a recombinant plant cell line, plant tissue or plant with an expression vector having an abiotic environmental stress-responsive promoter of SEQ ID NO: 27 and the gene downstream thereof; and (b) culturing and cultivating the recombinant plant cell, plant tissue or plant under an abiotic environmental stress, wherein the promoter regulates the expression of the gene under the abiotic environmental stress.
摘要翻译：本发明提供一种通过（a）用具有SEQ ID NO：27的非生物环境胁迫应答启动子和其下游基因的表达载体制备重组植物细胞系，植物组织或植物来调节基因表达的方法 ; 和（b）在非生物环境胁迫下培养和培养重组植物细胞，植物组织或植物，其中所述启动子调节所述基因在非生物环境胁迫下的表达。

18. 发明申请

US20050214808A1 Plant having tolerance to environmental stress 有权
标题翻译：植物对环境胁迫具有耐受性
公开(公告)号：US20050214808A1
公开(公告)日：2005-09-29
申请号：US10994235
申请日：2004-11-23
申请人： Taishi Umezawa , Kazuo Shinozaki
发明人： Taishi Umezawa , Kazuo Shinozaki
IPC分类号： A01H5/00 , C07H21/04 , C12N9/12 , C12N15/09 , C12N15/82 , C12Q1/68
CPC分类号： C12N15/8273 , C12N9/12 , C12N15/8271
摘要： This invention provides a stress tolerant transgenic plant with the utilization of a signal transduction factor located upstream of the gene group associated with environmental responses. This invention provides a gene encoding the following protein (a) or (b) and a stress tolerant transgenic plant to which such gene has been introduced: (a) a protein consisting of the amino acid sequence as shown in SEQ ID NO: 2 in the Sequence Listing; or (b) a protein consisting of an amino acid sequence derived from the amino acid sequence as shown in SEQ ID NO: 2 in the Sequence Listing by deletion, substitution, or addition of one or several amino acid residues and having activity of inducing expression of stress responsive transcription factors and activity of protein kinase.
摘要翻译：本发明提供了一种利用位于与环境反应相关的基因组上游的信号转导因子的胁迫耐受转基因植物。本发明提供了编码以下蛋白质（a）或（b）的基因和已经引入该基因的胁迫耐受转基因植物：（a）由SEQ ID NO：2所示的氨基酸序列组成的蛋白质序列表; 或（b）由序列表中SEQ ID NO：2所示的氨基酸序列的氨基酸序列组成的蛋白质，通过缺失，取代或添加一个或几个氨基酸残基并具有诱导表达的活性的应激反应转录因子和蛋白激酶的活性。

19. 发明授权

US06753461B2 Method for increasing stress-resistance to a plant 有权
公开(公告)号：US06753461B2
公开(公告)日：2004-06-22
申请号：US09810506
申请日：2001-03-19
申请人： Teruaki Taji , Kazuo Shinozaki , Chieko Ohsumi
发明人： Teruaki Taji , Kazuo Shinozaki , Chieko Ohsumi
IPC分类号： C12N1582
CPC分类号： C12N9/1051 , C12N15/8245 , C12N15/8273
摘要： The present invention enables production of a plant resistant to environmental stresses including drought and high salt concentration. The invention increases stress resistance to a plant by increasing galactinol content in the plant.

20. 发明授权

US4847221A AlN sintered body having high thermal conductivity and a method of fabricating the same 失效
标题翻译：具有高导热性的AlN烧结体及其制造方法
公开(公告)号：US4847221A
公开(公告)日：1989-07-11
申请号：US142818
申请日：1988-01-11
申请人： Akihiro Horiguchi , Fumio Ueno , Mitsuo Kasori , Yoshiko Sato , Masaru Hayashi , Hiroshi Endo , Kazuo Shinozaki , Akihiko Tsuge
发明人： Akihiro Horiguchi , Fumio Ueno , Mitsuo Kasori , Yoshiko Sato , Masaru Hayashi , Hiroshi Endo , Kazuo Shinozaki , Akihiko Tsuge
IPC分类号： C04B35/581
CPC分类号： C04B35/581
摘要： According to the present invention, there is provided an aluminum nitride sintered body having a high thermal conductivity and essentially consisting of a AlN single-phase, containing 0.01 to 8,000 ppm of rare earth elements and less than 2,000 ppm of oxygen. According to the present invention, there is provided a method of fabricating an aluminum nitride sintered body having a high thermal conductivity and essentially consisting of AlN single-phase, containing 0.01 to 8,000 ppm of rare earth elements and less than 2,000 ppm of oxygen, wherein a molded body prepared by mixing and molding an aluminum nitride power having less than 7 wt % of oxygen and an average particle size of 0.05 to 5 .mu.m and with rare earth compounds of 0.01 to 15 wt % of based on rare earth elements content, or a sintered AlN body containing oxide grain boundary phases of 0.01 to 15 wt % of rare earth elements and 0.01 to 20 wt % of oxygen and (rare earth element)--Al--O compounds phases and/or (rare earth element)--O compounds phases, is fired in a reducing atmosphere at a temperature of 1,550 to 2,050.degree. C. for 4 hours or more.
摘要翻译：根据本发明，提供了一种具有高导热性且基本上由含有0.01至8,000ppm的稀土元素和小于2,000ppm的氧的AlN单相组成的氮化铝烧结体。根据本发明，提供一种制造具有高导热性且基本由AlN单相组成的氮化铝烧结体的方法，该AlN单相含有0.01至8,000ppm的稀土元素和小于2,000ppm的氧，其中通过将少于7重量％的氧和平均粒径为0.05〜5μm的氮化铝粉末和基于稀土元素含量为0.01〜15重量％的稀土类化合物混合成型而制备的成型体，或含有0.01〜15重量％的稀土元素和0.01〜20重量％的氧和（稀土元素）-Al-O化合物相和/或（稀土元素）-Oi的氧化物晶界相的烧结AlN体化合物相在1550〜2050℃的温度下在还原气氛中烧成4小时以上。

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